fSlnmh dnshgh `lwm pzshkhy jhrm最新文献_第4页

Methicillin and Mupirocin resistance genes in Staphylococcus aureus isolated from hospital staff in Darab by using of Multiplex 多普勒法分离达拉市医务人员金黄色葡萄球菌耐甲氧西林和莫匹罗星基因

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-11-01 DOI: 10.52547/jmj.17.3.35

H. jamali

引用次数: 0

The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line 螺旋藻微藻提取物对脑癌症细胞Bcl-2抗凋亡基因表达的影响

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-11-01 DOI: 10.52547/jmj.17.3.17

M. Kavousi, delnaz fatemi

{"title":"The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line","authors":"M. Kavousi, delnaz fatemi","doi":"10.52547/jmj.17.3.17","DOIUrl":"https://doi.org/10.52547/jmj.17.3.17","url":null,"abstract":": U87 cell line contains glioblastoma cancer cells as the most acute type of human brain tumor. Increased expression of Bcl-2 gene in cancer cells has anti-apoptotic effect. In the present study the effect of Spirulina extract on gene expression in cancer cell line was investigated. In this cell The brain cancer cell line was procured and cultivated Iranian Biological Cells were divided into two treatment and control groups. Spirulina alga extract was affected on the treatment group and its toxicity was measured using MTT assay (compound 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor). The reference gene in this study was GAPDH. RNA was extracted from control and control cells and cDNA was made. The expression of genes was evaluated using ∆∆Ct in Real-time PCR. The result of MTT assay showed that the IC50 (Median Inhibition Concentration) value was 10.01 mg/ml for the 48 hour treatment. The result of Real-time PCR showed that the expression of Bcl-2 and the anti-apoptotic gene significantly decreased 3 folds in treatment group. Conclusion: According to the results of MTT assay and Real-time PCR, the expression of Bcl-2 significantly decreased 3 times in treatment group.","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41399813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Integrative Psychotherapy on Body Image, Mindful Eating and Satisfaction of Therapy and Therapist in Obese women 综合心理治疗对肥胖妇女身体形象、正念饮食及治疗师满意度的影响

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-11-01 DOI: 10.52547/jmj.17.3.7

Khadijeh Babakhani, S. Vaziri, Bouk Tajeri, F. Lotfi-Kashani, tahereh ranjbarpour

引用次数: 0

Identification of Campylobacter species in Jahrom slaughterhouse chickens in 2016-17 2016- 2017年贾罗姆屠宰场鸡弯曲杆菌种类鉴定

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-11-01 DOI: 10.52547/jmj.17.3.1

M. Pourahmadi, reyhaneh rouhijahromi, Mohsen Zargar, B. Haghighi, F. Moradi, Z. Faraji

引用次数: 0

Evaluation of intima-media thickness of common carotid artery in thalassemia patients compared to healthy individuals 地中海贫血患者与健康人颈总动脉内膜-中膜厚度的比较

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-05-01 DOI: 10.52547/jmj.17.2.1

O. Zekavat, F. Mehrabipour, N. Shakibazad, Z. Fattah, S. Haghpanah

引用次数: 0

Cloning, Optimization of Expression Condition and Purification of the Recombinant Nef- MPER-V3 Protein of HIV-1 in Prokaryotic Expression System HIV-1重组Nef- MPER-V3蛋白原核表达系统的克隆、表达条件优化及纯化

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-05-01 DOI: 10.52547/jmj.17.2.46

A. Faghih, S. M. Sadat, A. Bolhassani, S. Irani

引用次数: 0

Protective effect of melatonin on pituitary- gonadal axis hormones and spermatogenesis in sertraline-induced adult Balb/c mice 褪黑素对舍曲林诱导成年Balb/c小鼠垂体-性腺轴激素和精子发生的保护作用

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-05-01 DOI: 10.52547/jmj.17.2.54

M. Goudarzi, A. Pilehvarian, A. Karimi

引用次数: 0

Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer PCR-RFLP技术检测乳腺癌患者脂联素基因G276T多态性

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-05-01 DOI: 10.52547/jmj.17.2.8

Zahra Tahmasebi Fard

引用次数: 0

Frequency of bacterial contamination of platelet products in blood transfusion organization of Jahrom in 2016-17 2016- 2017年嘉龙输血机构血小板产品细菌污染频次分析

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-03-01 DOI: 10.52547/jmj.17.1.52

A. Kazemi, Fatemeh Atashi, Reyhneh Rouhi jahromi, D. Javeshghani, Pejman Hamedi-Asl, Fatemeh Vahidinezhad, Abdolrahman Mohtashemifar, Zahra Hooshyar, Z. Rezaee, Nazila Shiravani, Pegah Eidizadeh, Farnoosh Hatami

{"title":"Frequency of bacterial contamination of platelet products in blood transfusion organization of Jahrom in 2016-17","authors":"A. Kazemi, Fatemeh Atashi, Reyhneh Rouhi jahromi, D. Javeshghani, Pejman Hamedi-Asl, Fatemeh Vahidinezhad, Abdolrahman Mohtashemifar, Zahra Hooshyar, Z. Rezaee, Nazila Shiravani, Pegah Eidizadeh, Farnoosh Hatami","doi":"10.52547/jmj.17.1.52","DOIUrl":"https://doi.org/10.52547/jmj.17.1.52","url":null,"abstract":": Introduction: Most cases of blood transfusion-associated bacterial sepsis are caused by platelet concentrates (PCs). Screening of PCs is carried out in the Iranian quality control plans as it is essential for detection of bacterial contamination. With regard to quality control, this study investigated the frequency of bacterial contamination of PCs through Gram staining and microbial culture methods. In per for in agar and agar under aerobic and microaerophilic for detection of both Gram positive and negative cocci and Gram positive bacteria. Cultures were also made in MacConkey medium under aerobic conditions to identify Gram negative bacilli. In cases of bacterial growth and colony formation, differential tests were performed to detect the bacteria. bags collected from donors in Jahrom Blood Transfusion Organization, from which two platelet cord (880 samples) had been isolated on the first and third days. Accordingly, a contamination frequency of 0.23% was detected for platelet products in Jahrom city. Performing staining and culture from all of the platelet cords before are is a good to ensure safety and contamination quality","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46184489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm formation and antimicrobial resistance of uropathogenic Escherichia coli and Klebsiella pneumonia strains isolated from pregnant women in Kerman, Iran 伊朗克尔曼孕妇尿路致病性大肠埃希菌和肺炎克雷伯菌分离株的生物膜形成和耐药性

fSlnmh dnshgh `lwm pzshkhy jhrm Pub Date : 2019-03-01 DOI: 10.52547/jmj.17.1.15

S. Pooyan, N. Kazemi-Pour, Farokh Rokhbakhsh-Zamin

{"title":"Biofilm formation and antimicrobial resistance of uropathogenic Escherichia coli and Klebsiella pneumonia strains isolated from pregnant women in Kerman, Iran","authors":"S. Pooyan, N. Kazemi-Pour, Farokh Rokhbakhsh-Zamin","doi":"10.52547/jmj.17.1.15","DOIUrl":"https://doi.org/10.52547/jmj.17.1.15","url":null,"abstract":"Urinary tract infections are the common medical problems in pregnant women and associated bacteria produce biofilm which protects them from antibiotics. Aim of this study was to determine the pattern of antibiotic resistance and biofilm formation by Escherichia coli and Klebsiellapneumoniae isolated from urine of pregnant women in Kerman, Iran. 107 urine samples were collected from pregnant women. Identification was performed by biochemical tests. Antibiotic sensitivity by disc diffusion method and minimum inhibitory concentration (MIC) by agar dilution method were performed. The cell surface hydrophobicity (CSH) behavior of those strains and their ability to form biofilm on glass and propylene surfaces was evaluated in static and shake conditions. The effect of antibiotics on biofilm formation ability was also determined on the catheter surface. From 80 positive cases, 35 isolates of Escherichia coli and 15 isolates of Klebsiella pneumoniae were selected for this research. The Maximum resistance and sensitivity of isolates were observed about amoxicillin and imipenem, respectively. All isolates with CSH higher than 70% showed better biofilm formation even though on propylene in shaking state. Treatment of culture with 0.5 MIC (µg/ml) and 0.25 MIC (µg/ml) of selected antibiotics significantly reduced the adhesion ability of isolates. way against UTI in pregnancy.","PeriodicalId":33852,"journal":{"name":"fSlnmh dnshgh `lwm pzshkhy jhrm","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41779783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1