Cloning, Optimization of Expression Condition and Purification of the Recombinant Nef- MPER-V3 Protein of HIV-1 in Prokaryotic Expression System

Abstract

a clear band of around 35 kDa in SDS-PAGE and was detectable using anti-Neff antibody in western blotting. Conclusion: Our results showed that the Nef-MPER-V3 protein was successfully expressed in prokaryotic system and purified protein may provide the antigen for future experiments for immunogenicity evaluation in mice is currently undertaken.