Health and Ecology Issues最新文献

{"title":"Immunological features of acute cytomegalovirus infection in immunocompetent patients","authors":"M. L. Dotsenko, E. G. Fomina, I. Y. Hutsaliuk, E. E. Grigorieva","doi":"10.51523/2708-6011.2023-20-4-07","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-4-07","url":null,"abstract":"Objective. To identify and evaluate immunological features in immunocompetent patients with acute cytomegalovirus infection (CMVI).Materials and methods. Thirty-three immunocompetent adult patients with acute cytomegalovirus infection were included in the study; 4 of them had thrombotic complications. The control group consisted of 10 healthy donors. All 33 blood samples were tested by flow cytometry. The content of antigen-specific cells was checked in all patients. Results. All patients had positive cytomegalovirus DNA PCR tests. The content of activated T cells in patients with acute CMV infection and thrombotic complications is 7.7 times higher (p < 0.001) than in conditionally healthy patients (median values: 36.02% (31.01; 47.92) and 4.68% (3.39; 5.25), respectively). The content of granulocytic myeloid suppressor cells (G-MLSC) in patients with acute CMV infection and thrombosis exceeds the same indicator in the group of healthy volunteers by 8.3 times (p < 0.001) (median values were 0.38% (0.24; 0.54) and 0.05% (0.03; 0.07) respectively). The number of regulatory T cells in patients with acute CMV infection and thrombosis was reduced by 3.1 times (p < 0.001) compared to the same indicator in the group of healthy volunteers (median indicators: 0.79% (0.57; 1.09) and 2.45% (2.01; 3.86), respectively). Immunophenotyping of CD3+ cells showed a tendency to increase the proportion of more mature cells, namely effector memory cells (TEM) and terminally differentiated memory cells (TEM RA) with a decrease in the percentage of “naive” cells.Conclusion. A high level of antigen-specific T-cell response and a low content of T-regulatory cells may indicate insufficient control of the proliferation of T-cytotoxic lymphocytes, which may contribute to the long-term persistence of the virus and the development of chronic inflammation of the vessel wall, which requires further study. Thus, in patients with acute CMV infection, an additional risk factor for thrombosis appears, which must be taken into account when carrying out therapeutic and diagnostic measures.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"107 S2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139630262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Locoregional recurrences of breast cancer after reconstructive plastic and organ-preserving surgery","authors":"O. N. Ganusevich, T. N. Nesterovich, I. V. Fedarkevich, S. L. Achinovich, D. M. Los’, I. V. Kontsevenko","doi":"10.51523/2708-6011.2023-20-4-06","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-4-06","url":null,"abstract":"Objective. To analyze locoregional relapses (LRR) after reconstructive plastic and organ-preserving operations in breast cancer (BC)Materials and methods. A retrospective analysis of the results of the treatment of 303 patients with BC who underwent reconstructive plastic and organ-preserving operations in the period from 2014 to 2019 in the Oncological Department of General Oncology and Rehabilitation of Gomel Regional Clinical Oncological Dispensary was carried out. The median age was 44 years (25-70 years). Statistical data processing was carried out using the application software package “Statistica”, 10.0. To characterize the surveyed groups, a standard methodological approach based on the calculation of descriptive statistics data was used. In order to statistically assess the relationship between the frequency of relapses depending on the stage and the molecular biological type of neoplasm, the Spearman correlation coeffi cient was used. The accepted level of statistical signifi cance (p) was 0.05.Results. Locoregional relapses were recorded in 11 (3.6%) patients, of which local — in 8 (2.6%), regional — in 3 (1.0%). The minimum period of development of LRR was 15 months, the maximum was 74 months; the median period of occurrence of LRR was 47 months. Depending on the stage of breast cancer, relapses developed: at stage I — in 4 (3.7%) cases, II — 5 (3.5%), III — 2 (4.2%). Depending on the molecular biological subtype of the neoplasm, LRR were distributed as follows: with luminal A — 1 (1.3%) case, luminal In HER2-negative — 1 (0.9%), luminal In HER2-positive — 6 (9.7%), non-luminal HER2-positive 1 (6.3%), three times negative — 2 (5.6%). Discordance of the receptor status was detected in 8 (72.7%) patients with recurrent tumor, most often due to the loss or decrease in the number of progesterone receptors (PR). The level of tumor-infi ltrating lymphocytes (TILs) in primary neoplasm ranged from 4 to 12%, in recurrent tumors it remained low: 5-10%.Conclusion. Given the heterogeneity of BC, the risk of developing LRR depends on many factors. LRR developed in luminal HER2-positive cancer in 9.7% of cases (p 0.05), stage III breast cancer — 4.2% (p 0.05). One of the important predictive factors is the evaluation of TILs. We noted a low level of TILs in both primary and recurrent tumors. There is a high discordance in the receptor status — 72.7%, which is important to take into account when prescribing systemic therapy.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139536236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved method of hemostasis for the middle rectal artery","authors":"A. V. Kuzmenko, V. N. Zhdanovich","doi":"10.51523/2708-6011.2023-20-4-05","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-4-05","url":null,"abstract":"Objective. To obtain data on the comparative characteristics of traditional and improved methods of hemostasis for the middle rectal artery (MRA).Materials and methods. The material for the sectional study was 206 corpses of men (the age of the deceased was from 22 to 82 years, the average age was 57 years) and 113 corpses of women (the age of the deceased was from 32 to 93 years, the average age was 63 years). To achieve the purpose of the study, the following methods were used: vascular injection, dissection, statistical processing. 85 patients of both sexes aged from 57 to 75 years were selected to conduct the clinical part of the study at Gomel Regional Specialized Clinical Hospital. Among them, 50 people were in the comparison group and 35 people were in the main group. All patients were diagnosed with stage I or II rectal cancer. The operative technique for performing hemostasis on the MRA during anterior rectal resection in the comparison group included sequential ligation and stitching between the clamps first of the distal third of the length of this artery, and then its proximal third. In the main group, the method of hemostasis for MRA was supplemented by ligation and stitching of this vessel in the middle third of its length. Statistical data processing included parametric and nonparametric methods of comparative analysis.Results. On cadaveric material, it was found that most of the extraorgan anastomoses of the MRA both in men and women have been localized in the middle third of this artery. The results obtained during the clinical study demonstrate the superiority of the improved hemostasis method for MRA over the traditional method. The proposed method includes additional ligation and stitching of the middle third of the MRA.Conclusion. The improved hemostasis method for MRA in anterior rectal resection is highly effective, which makes it possible to recommend it as the method of choice in the surgical treatment of upper ampullary rectal cancer of stage I or II. ","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"16 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139536292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the structure of congenital malformations in newborns","authors":"O. А. Zaitseva, L. S. Sergeychik","doi":"10.51523/2708-6011.2023-20-4-04","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-4-04","url":null,"abstract":"Objective. To conduct an analytical assessment of the pattern of congenital malformations in newborns born in 2017 and 2021 in a hospital-based healthcare organization, taking into account the anamnesis of mothers as a possible cause of the formation of a malformation in the fetus.Materials and methods. A retrospective analysis of 90 developmental histories of newborns delivered between January and December 2017 (first group) and 100 histories of newborns born between January and December 2021 (second group) in the maternity hospital of the Gomel Regional Clinical Hospital was conducted.Results. The leading place among congenital malformations (CM) in the studied two groups is occupied by congenital heart defects (CHD) and defects of the urinary system. In the group of newborns with CM delivered in 2017, boys (60%) prevailed, in 2021 — girls (58%). Mothers of the children from the first comparison group were more likely to live in cities (77.7%). When analyzing the extragenital pathology of mothers, data were obtained that the mothers of the children of the first study group most often suffered from cardiovascular, endocrine diseases, diseases of the urinary system. In mothers of the children of the second comparison group, concomitant chronic diseases came out on top. The structure of maternal diseases during pregnancy is diverse, but the only statistically significant indicator was COVID-19 infection, which affected the mothers during pregnancy in 2021. The data on the diagnosis of CM in the fetus by ultrasound examination of a pregnant woman are statistically insignificant, it is impossible to draw a conclusion about the improvement or deterioration of the quality of early diagnosis of CM. The percentage of diagnosis of CM prenatally in 2017 and 2021 was 33.3 and 40%, respectively.Among CHD in percentage, the leading place in both groups is occupied by a defect of the interventricular septum ― 67.7 and 51%. In the structure of defects of the urinary system, a statistically significant defect is kidney dystopia in 2021 (28%). When analyzing malformations of the musculoskeletal system, all indicators were statistically insignificant. Conclusion. As a result of the analysis, it was found that the stable leadership among the CM belongs to the CHD and the CM of the urinary system. The frequency of diagnosis of CM in the fetus by ultrasound examination of pregnant women remains at a fairly low level. Chronic diseases of mother, diseases of the cardiovascular system, endocrine and urinary system, COVID-19 suffered by mother during pregnancy may be a possible cause of the formation of CM in the fetus.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"15 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139630664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels of interleukins-1β, -2, -6, -8 in the blood of patients with local wounds at various stages of the infectious process","authors":"Y. Yarets","doi":"10.51523/2708-6011.2023-20-4-02","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-4-02","url":null,"abstract":"Objective. To evaluate the levels of interleukins (IL) -1β, -2, -6, -8 in the blood serum of patients with wounds, depending on the clinical and microbiological characteristics of the wound process.Materials and methods. The evaluation of IL-1ß, IL-2, IL-6, IL-8 indices in the blood serum of 206 patients with wounds was carried out. When analyzing changes in IL, the clinical and microbiological characteristics of the wound process were taken into account.Results. Changes in the levels of IL-1ß, IL-2, IL-6 and IL-8 in the blood of patients with wounds are determined by the duration of the wound process, the presence of clinical signs of inflammation and the presence of microorganisms in the wound. The absence of a systemic response to the presence of potential pathogens (S. aureus) in an acute wound, when IL levels do not exceed 10 pg/ml, can serve as an additional criterion for contamination with minimal wound life (up to 4 days). Preservation of high values of IL-8 from 38.16 pg/ml (33.31; 42.11), no changes in IL-2 (no more than 2.23 pg/ml (1.41; 4.01)) in the blood of patients with wounds which duration exceeds 22 days is a sign of a violation of the healing process and the formation of a chronic wound. The level of IL-6 is recommended as an additional criterion determining the progression of the infectious process in patients with wounds, when an increase in IL-6 values is recorded from 18.79 pg/ml (15.71; 23.01) at critical colonization to 51.65 pg/ml (35.19; 51.95) at the stage of wound infection. IL-6 values of no more than 10 pg/ml indicate the absence of an active inflammatory process.Conclusion. The levels of proinfl ammatory interleukins in the blood of patients can serve as additional laboratory criteria determining the disruption of wound healing and progression of the wound infection process.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"41 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139536518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation-induced changes in the properties and functions of fibroblasts","authors":"T. D. Matveyenkava, I. Nazarenko, А. М. Yurkovskiy, M. Starodubtseva","doi":"10.51523/2708-6011.2023-20-4-01","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-4-01","url":null,"abstract":"This review is devoted to the analysis of the literature containing experimental and clinical data on radiation-induced changes in connective tissue and its cellular component to create an overall picture of the leading mechanisms of radiation fibrosis development.The review analyzed publications for the period 1995-2022, presented in three academic databases: Scopus, PubMed and Web of Science. In the search process, various combinations of logical operators (or, and, not) were used to combine search keywords (ionizing radiation, connective tissue, fibroblasts) to find relevant studies in academic databases. The development of radiation fibrosis is determined by radiation-induced changes in the properties and functions of fibroblasts. The article discusses the main biological effects of irradiation of fibroblastic cells with X-ray, gamma and alpha radiation, presents the mechanisms of radiation-induced differentiation in the fibroblast/myofibroblast system, and also notes the role of connective tissue microenvironment and oxidative stress associated with mitochondria in this process. The main experimentally established effects presented in the review and the general scheme of the mechanisms of non-lethal action of ionizing radiation on fibroblasts contribute to a better understanding of the mechanisms of radiation-induced fibrosis development","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139536830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Dmitry V. Cherednichenko «Ethical recommendations and legal standards for the registration of consent and refusal of the patient in relation to medical intervention»","authors":"A. Editorial","doi":"10.51523/2708-6011.2023-20-3-18","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-3-18","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139335301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the level of endogenous respiration of the small intestine mucosa at various times after irradiation","authors":"N. S. Myshkavets","doi":"10.51523/2708-6011.2023-20-2-10","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-2-10","url":null,"abstract":"Objective. To study the level of endogenous respiration of the small intestine mucosa of experimental animals after exposure to external irradiation at a dose of 0.5 and 1.0 Gy.Materials and methods. The study was conducted on white male outbred rats weighing 180-200 g. The animals were irradiated once in the “IGUR-1” unit, 137Cs source, dose rate 0.92 Gy/min. The parameters of tissue respiration were studied by polarographic method using Record-4 device (RF) in a 2 ml cell with a closed platinum Clark electrode at 25°C.Results. It was shown that changes in respiratory activity over time are non-monotonous. This confirms the high sensitivity of the small intestine to radiation exposure.Conclusion. The level of endogenous respiration of the small intestine mucosa can be one of the criteria for assessing the energy status of the tissue after irradiation.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116523687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction of cervical insufficiency during prolapse of the fetal bladder and intrauterine infection","authors":"T. N. Zakharenkova, A. V. Kruglikova, E. A. Voronkova","doi":"10.51523/2708-6011.2023-20-2-16","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-2-16","url":null,"abstract":"This article describes a clinical case of the course and management of pregnancy complicated by cervical insufficiency with prolapse of the fetal bladder and formation of “sludge” in the amniotic fluid at 20–21 weeks of pregnancy. Gradual use of antibacterial therapy and hormonal support with micronized progesterone, subsequent surgical serclage and the installation of an unloading obstetric pessary allowed to prolong pregnancy to 284 days and to deliver through the natural birth canal with a newborn without signs of intrauterine infection.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124194892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential diagnosis of acromegaly and pachydermoperiostosis","authors":"N. V. Kholupko, E. Vashchenko, A. Makhlina, Yana Naumenava, A. Razhko","doi":"10.51523/2708-6011.2023-20-2-19","DOIUrl":"https://doi.org/10.51523/2708-6011.2023-20-2-19","url":null,"abstract":"Acromegaly is a neuroendocrine disease with diverse clinical manifestations that develop over a long period. In the process of examining a patient, it may be necessary to conduct a differential diagnosis of acromegaly with various syndromes and conditions, including such a rare disease as pachydermoperiostosis. Features of the clinical presentation and diagnosis are given in the description of a clinical case from our practice.","PeriodicalId":336889,"journal":{"name":"Health and Ecology Issues","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127887906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}