Digital Chinese Medicine最新文献

{"title":"Mechanisms of Yangxin Tongmai Formula for blood stasis syndrome in coronary heart disease rats based on untargeted plasma metabolomics and intestinal flora 16S rRNA sequencing","authors":"Liu Yinxing ,&nbsp;Chen Zijun ,&nbsp;Wang Yiqin ,&nbsp;Cheng Xihua ,&nbsp;Li Jie ,&nbsp;Chen Lingli","doi":"10.1016/j.dcmed.2023.07.009","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.07.009","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the correlations between intestinal flora, plasma metabolites, and blood stasis syndrome in coronary heart disease (CHD), and the mechanisms of Yangxin Tongmai Formula (养心通脉方, YXTMF) for blood stasis syndrome in CHD rats.</p></div><div><h3>Methods</h3><p>A total of 18 specific pathogen free (SPF) male Sqrague-Dawley (SD) rats were used to establish CHD rat models with blood stasis syndrome, which were then randomized into model, YXTMF, and atorvastatin calcium (AVT) groups, with six rats in each group, and were intervened through gavage for two weeks. Subsequently, additional six rats that received normal diet were included as normal group. The pathological changes in the CHD rat models were identified by hematoxylin-eosin (HE) staining. The electrocardiogram, hemodynamics, and lipid profiles of the rats were detected as well. The untargeted plasma metabolomics of rats were analyzed by liquid chromotography-tandem mass spectrometry (LC-MS/MS), their ileal mucosal flora by 16S rRNA sequencing, and the correlation between the two results were also analyzed.</p></div><div><h3>Results</h3><p>The whole blood viscosity, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) of rats in the model group increased compared with those in the control group (<em>P</em> &lt; 0.05). In the model group, the proliferation of endothelial cells in the coronary artery of rats was damaged, with quite a few vacuolated pathological changes observed. However, the endothelial lesions in the coronary artery of rats were alleviated in the intervention groups (YXTMF and AVT groups). With the use of LC-MS/MS, a total of 33 potential endogenous metabolites were identified in plasma, among which 1-methylhistidine, N-acetylhistamine, progesterone, and deoxycorticosterone were expected to be the differential metabolites in CHD rats with blood stasis syndrome. The 16S rRNA sequencing results showed that improved diversity and abundance of intestinal flora were observed in the YXTMF group. The correlation analysis suggested that <em>Hydrogenophaga</em>, <em>Limnohabitans</em>, and <em>Polaromonas</em>, which were highly related to the formation of blood stasis syndrome in CHD patients, were positively correlated with plasma metabolites such as 5-hydroxyindole, N-acetylhistamine, and progesterone (<em>P</em> &lt; 0.01), but were negatively correlated with plasma metabolites such as L-arginine, homoarginine, and Boc-beta-cyano-L-alanine (<em>P</em> &lt; 0.01). After YXTMF intervention, <em>Lactobacillus</em>, <em>Corynebacterium</em>, and <em>Candidatus Nitrososphaera</em> were positively correlated with plasma metabolites such as Boc-<em>β</em>-cyano-L-alanine, stachydrine, and naringenin (<em>P</em> &lt; 0.05), while negatively correlated with 5-hydroxyindole, N-acetylhistamine, and oleoylethanolamide (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>YXTMF could alleviate blood stasis syndrome in CHD rats through ","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 2","pages":"Pages 198-209"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49749662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Wuyao (Linderae Radix) in treating chronic pelvic inflammatory disease based on network pharmacology and animal experiments","authors":"Xie Mingxia ,&nbsp;Yan Jing ,&nbsp;Qing Dongqin ,&nbsp;Zhu Zhengqing ,&nbsp;Du Ke ,&nbsp;Wang Xiaoye ,&nbsp;Wang Hanqing","doi":"10.1016/j.dcmed.2023.07.010","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.07.010","url":null,"abstract":"<div><h3>Objective</h3><p>To predict the active components and action targets of Wuyao (Linderae Radix) in the treatment of chronic pelvic inflammatory disease (CPID) based on network pharmacology, explore possible mechanisms of the treatment through animal experiments, and provide a scientific basis for clinical applications of Wuyao (Linderae Radix).</p></div><div><h3>Methods</h3><p>Possible active components and targets of Wuyao (Linderae Radix) in the treatment of CPID were obtained applying network pharmacology and molecular docking technology. CPID rat models were established using the mixed <em>Escherichia coli</em>, <em>Staphylococcus aureus,</em> and <em>Ureaplasma urealyticum</em> plus the performance of mechanical injury. Hematoxylin-eosin (HE) staining was applied to observe the pathological changes in the uterus, fallopian tube, and spleens of rat models. The contents of nitric oxide (NO), superoxide dismutase (SOD), and malondialdehyde (MDA) in the serum of rats were determined with the use of corresponding detection kits. Enzyme-linked immunosorbent assay (ELISA) test was used to measure the expression of interleukin (IL)-6 and IL-10 in the serum of rat models. Flow cytometry was used to determine the percentage of CD4⁺ and CD8a⁺ T cells as well as CD4⁺ CD25⁺ regulatory T cells (Tregs) in the spleen of rat models.</p></div><div><h3>Results</h3><p>A total of nine potential active components and four core therapeutic targets related to inflammatory response in Wuyao (Linderae Radix) were obtained. The animal experiments showed that Wuyao (Linderae Radix) markedly inhibited uterus swelling, regulated morphological changes in the fallopian tube and spleen, effectively reduced inflammatory infiltration and injuries in the uterus and fallopian tube, and improved spleen functions in CPID rats. Moreover, Wuyao (Linderae Radix) markedly reduced the levels of NO, IL-6, and MDA, and increased the levels of IL-10 and SOD in the serum of rats. Wuyao (Linderae Radix) also elevated the percentage of CD4⁺ T cells and the CD4⁺ T/CD8a⁺ T cell ratio, reduced the percentage of CD8a⁺ T cells, and raised the percentage of CD4⁺ CD25⁺ Tregs that had been abnormally decreased in rat models (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>Wuyao (Linderae Radix) could have therapeutic effects on CPID rats by relieving oxidative stress, mitigating inflammatory levels, and regulating the immuno-function of T cell subgroups to improve the pathological changes in CPID rats. It is a medicinal herb worth being further explored for its clinical values.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 2","pages":"Pages 210-220"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49749859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Schrödinger equation and its application in traditional Chinese medicine","authors":"W.A.N. Xiaohai","doi":"10.1016/j.dcmed.2023.03.002","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.03.002","url":null,"abstract":"","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 1-2"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49753123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure-based virtual screening to predict Loxosceles spider venom natural inhibitors","authors":"Haitham Ahmed Al-Madhagi","doi":"10.1016/j.dcmed.2023.03.001","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.03.001","url":null,"abstract":"<div><h3>Objective</h3><p><em>Loxosceles</em> spider bite accidents are rising in wide areas of the world which necessitates the exploration of natural inhibitors to inhibit the most significant enzymes, namely sphingomyelinase D (Smase D) and hyaluronidase.</p></div><div><h3>Methods</h3><p>Virtual screening using traditional Chinese medicine (TCM) against Smase D (PDB ID: <span>2F9R</span><svg><path></path></svg>) and hyaluronidase was performed by the DrugRep server. Absorption, distribution, metabolism, and excretion (ADME) parameters were predicted via the same server. In addition, molecular dynamics (MD) simulation was conducted using CABS-flex 2.0 tool to prioritize the best potential natural inhibitors.</p></div><div><h3>Results</h3><p>Tiliroside and Digitoxin were the best natural inhibitors from TCM to Smase D and hyaluronidase in terms of molecular docking and ADME parameters, while Digitoxin and <em>β</em>-carotene were the most potent inhibitors against hyaluronidase. MD simulations demonstrated the stability of the docked complexes.</p></div><div><h3>Conclusion</h3><p><em>In-silico</em> inhibition of <em>Loxosceles</em> spidervenom enzymes through TCM was demonstrated, which deserves wet-lab experimentation.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 67-72"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49767506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual analysis of research hotspots and trends of external therapies in traditional Chinese medicine for insomnia","authors":"C.H.E.N. Shupeng ,&nbsp;T.A.N.G. Nana ,&nbsp;W.A.N.G. Simeng ,&nbsp;L.I.U. Yinghua ,&nbsp;Z.H.A.N.G. Zhiyong ,&nbsp;C.H.E.N. Shiyu","doi":"10.1016/j.dcmed.2023.02.003","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.02.003","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the current status and development trend of research on external therapies in traditional Chinese medicine (TCM) for insomnia over the past 10 years through bibliometrics and visual analysis, to provide references for further research on the topic.</p></div><div><h3>Methods</h3><p>Literature relating to TCM external therapies for insomnia from January 1, 2012 to December 31, 2021 was retrieved from Chinese databases, including China National Knowledge Infrastructure (CNKI), Wanfang Database, China Science and Technology Journal Database (VIP), and from the Web of Science Core Collection (WOSCC) for English articles. CiteSpace, VOSviewer, Scimago Graphica, and NoteExpress software were used to analyze publication volumes of the papers and how they were distributed in different journals, as well as to visualize the data of the countries, authors, institutions, and keywords.</p></div><div><h3>Results</h3><p>A total of 6 085 papers were obtained, of which 5 592 were from the Chinese databases and 493 were from the English database, with their publication volumes growing steadily year on year. Approximately 45 countries and regions were found to have published research on the topic. In terms of Chinese publications, the author with the most papers published was CHEN Yunfei from Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine. The closest collaboration was between LIU Chengyong from the Affiliated Hospital of Nanjing University of Chinese Medicine and YUE Zenghui from Hunan University of Chinese Medicine. In terms of English publications, the author with the most papers published was MAO Junj from Sloan-Kettering Cancer Research Center, USA, and LAO Lixing from the University of Hong Kong was his closest partner in collaboration. Heilongjiang University of Chinese Medicine was the institution with the most Chinese publications, and Shanghai University of Traditional Chinese Medicine was the one with the most English papers published. Studies on the topic were published in 386 Chinese journals and 205 English journals, respectively. Nowadays, the clinical application of TCM external treatments for insomnia, the selection of meridians and acupoints, therapies for insomnia and its related diseases are research hotspots. The combined use of different TCM external therapies is a trend in the treatment of insomnia and its concomitant diseases, especially in the fields of oncology, nursing, and psychiatric disorders. The exploration of mechanisms of TCM external therapies for insomnia is also a key direction for future research. In clinical practice, the commonly used external therapies for insomnia include acupuncture, ear-acupressure with beans, acupoint application, etc. The commonly selected acupoints are auricular points, Sishencong (EX-HN1), Shenmen (HT7), etc. The frequently studied meridians are Ren, Du, Qiao, etc. The insomnia concomitant","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 41-54"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49767505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of basic principles and methods of acupuncture standardization in traditional Chinese medicine","authors":"Guo Yi ,&nbsp;L.I. Zhenji ,&nbsp;Liu Baoyan ,&nbsp;Sang Binsheng ,&nbsp;F.U. Qiang ,&nbsp;Zhao Xue ,&nbsp;Chen Bo ,&nbsp;Chen Zelin ,&nbsp;Yang Huayuan ,&nbsp;H.E. Liyun ,&nbsp;Yang Yi ,&nbsp;L.V. Zhongqian ,&nbsp;Zhao Tianyi ,&nbsp;L.I. Dan ,&nbsp;F.U. Hua ,&nbsp;Yuan Xinru","doi":"10.1016/j.dcmed.2023.04.001","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.04.001","url":null,"abstract":"<div><p>Standardization is the universal language of the world, and standardization of traditional Chinese medicine (TCM) is essential for its communication in China and globally. However, the principles and methods of TCM acupuncture standardization have been unclear and inadequate in the early stages. Based on an investigative approach to understanding the current status, identifying problems, and finding solutions, our team has established basic principles of TCM acupuncture that embody Chinese wisdom, evaluated the international strategic environment systematically, proposed the principle of “importance of harmony and exercise of impartiality”, and established basic working principles. A series of methods for TCM acupuncture standard development and evaluation have been constructed, including general standards for the revision of TCM acupuncture standards, the first TCM acupuncture clinical research management specification, a shared full chain technology platform, a data center, and an evaluation research base for TCM acupuncture clinical research. Evaluation criteria for ancient literature and expert experience, a recommendation method for the “three main and three auxiliaries” TCM guideline for prevention were established, and quantifiable assessment methods of TCM standard applicability were proposed. These findings provide methodological guidance for TCM acupuncture standardization.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 3-8"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49753124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressant-like active ingredients and their related mechanisms of functional foods or medicine and food homologous products","authors":"Y.E. Tian ,&nbsp;X.U. Mengtao ,&nbsp;F.A.N.G. Jingpeng ,&nbsp;W.U. Qinxuan ,&nbsp;Z.O.U. Xiaoyan ,&nbsp;Y.A.N. Fangqin ,&nbsp;Q.I.N.G. Zhixing","doi":"10.1016/j.dcmed.2023.02.001","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.02.001","url":null,"abstract":"<div><h3>Objective</h3><p>To provide a new idea for the treatment of depression by summarizing the antidepressant effect and mechanism of active ingredients in functional food, and medicine and food homologous products.</p></div><div><h3>Methods</h3><p>The literature related to the antidepressant of functional food or medicine and food homologous products from September 25, 1996 to September 5, 2022 was collected through PubMed, Google Academic, Web of Science, and China National Knowledge Infrastructure (CNKI) databases. After that, their antidepressant active ingredients and mechanism of action were systematically summarized and analyzed.</p></div><div><h3>Results</h3><p>A total of 146 pieces of literature were involved in the study, including 67 plant-derived functional foods or medicine and food homologous products, 32 antidepressant extracts (including 8 flavonoid extracts), and 87 antidepressant active ingredients. The 87 antidepressant active ingredients include 7 terpenes, 22 saponins, 15 flavonoids, 11 phenylpropanoids, 7 phenols, 6 sugars, 8 alkaloids, and 11 others.</p></div><div><h3>Conclusion</h3><p>The study summarized and analyzed the active ingredients and mechanisms of antidepressants in functional foods and medicine and food homologous products, which provides a new vision for the development of new antidepressants and a potential alternative treatment for patients with depression.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 9-27"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49767503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative polypharmacokinetics of nine anti-inflammatory components of Jinyinhua (Lonicerae Japonicae Flos) and Shanyinhua (Lonicerae Flos) in mice with p-xylene-induced ear edema","authors":"L.I. Haiying ,&nbsp;X.I.A.O. Meifeng ,&nbsp;P.A.N. Xue ,&nbsp;L.I. Wenjiao ,&nbsp;Z.H.O.U. Yiqun ,&nbsp;L.I.U. Wenlong ,&nbsp;H.E. Fuyuan","doi":"10.1016/j.dcmed.2023.02.009","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.02.009","url":null,"abstract":"<div><h3>Objective</h3><p>To reveal the integral <em>in vivo</em> polypharmacokinetics (PPK) similarity or difference between Jinyinhua (Lonicerae Japonicae Flos, LJF) and Shanyinhua (Lonicerae Flos, LF), and provide reference for their clinical application.</p></div><div><h3>Methods</h3><p>The PPK model and its total quantum statistical moment similarity (TQSMS) method were used to compare the integral PPK profiles of nine components with anti-inflammatory efficacy (rutin, caffeic acid, chlorogenic acid, cryptochlorogenic acid, dispsacoside B, macranthoidin B, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C) of LJF and LF. A total of 54 Specific Pathogen Free (SPF) grade Kunming (KM) mice were randomized into LJF group and LF group (<em>n</em> = 27), and each group was divided into nine subgroups (<em>n</em> = 3) according to different time points. Subsequently, mice model of p-xylene-induced ear edema was constructed by oral administration of LJF and LF. The concentrations of the nine anti-inflammatory components in plasma samples of the mice were determined by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). And the pharmacokinetics (PK) parameters of single component and the integral PPK parameters [total quantum statistical moment (TQSM) and TQSMS] of multiple components were calculated by Drug And Statistics (DAS) software and home-brew programs with Excel, respectively.</p></div><div><h3>Results</h3><p>There were significant differences in single-component PK parameters between LJF and LF (<em>P</em> &lt; 0.05). Whereas, no significant differences were found in multi-component TQSM parameters, including total quantum zero moment (<em>AUC</em>_{<em>T</em>0<em>-t</em>}, <em>AUC</em>_{<em>T</em>0<em>-∞</em>}) and total quantum first moment (<em>MRT</em>_{<em>T</em>0<em>-t</em>}, <em>MRT</em>_{<em>T</em>0<em>-∞</em>}) for the total quanta (<em>P</em> &gt; 0.05). Accordingly, single-component TQSMS varied from 0.220 4 to 0.968 9, and that for the total quanta was 0.828 4, suggesting no significant differences in the speed and extent of bioavailability between LJF and LF. Furthermore, in light of high TQSMS (0.828 4), the integral PPK profiles of the nine anti-inflammatory components of LJF and LF were similar under 90% confidence intervals.</p></div><div><h3>Conclusion</h3><p>The PPK model and its TQSMS method are appropriate and efficient to compare the similarity or difference of integral PPK profiles of multi-component herbal medicines. It is suggested in this research that LJF can be replaced with LF or vice versa for anti-inflammatory treatment.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 73-85"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49753266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hotspots and comparative analysis of international acupuncture research over the past decade via Web of Science","authors":"S.U.N. Meiling ,&nbsp;Z.H.A.O. Fanghua ,&nbsp;G.A.O. Man ,&nbsp;Z.H.E.N. Siyuan ,&nbsp;C.H.E.N. Yueling ,&nbsp;Ramon Maria Calduch ,&nbsp;L.I. Haiyan","doi":"10.1016/j.dcmed.2023.02.002","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.02.002","url":null,"abstract":"<div><h3>Objective</h3><p>This study aims to summarize the hotspots of acupuncture research in China and abroad in recent 10 years.</p></div><div><h3>Methods</h3><p>The core collection of Web of Science was retrieved, and the literature on experimental and clinical research of acupuncture in China and abroad was collected from January 1, 2012 to December 31, 2021. The included literature was visually analyzed using CiteSpace 6.1.R3. Statistical analysis was carried out in the annual number of papers, authors, countries, and journals. According to the author's country, the literature was divided into Chinese and global categories for keywords clustering analysis and co-citation references analysis independently to obtain hotspots and trends in acupuncture research.</p></div><div><h3>Results</h3><p>There are 1 215 articles in acupuncture experimental medicine, 3 805 articles in acupuncture clinical medicine in China; 551 articles in acupuncture experimental medicine, and 4 538 articles in acupuncture clinical medicine abroad. The number of articles on acupuncture abroad remains stable and flat, whereas the annual number of domestic papers shows a continuously growth trend. LIU Cunzhi from Beijing Hospital of Traditional Chinese Medicine affiliated to Capital Medical University in China is the author who has published the most articles in the field of acupuncture in the recent decade. China is the most productive country in publishing such research articles. <em>Evidence-based Comprehensive and Alternative Medicine</em> is the journal with the most published articles. The mechanisms of acupuncture on nerve regeneration or nerve recovery and research on the mechanisms of acupuncture analgesia are hotspots in experimental acupuncture research, and the treatment of neuropathic pain and dysfunctional diseases with acupuncture are hotspots in clinical acupuncture research in China. Research on the mechanism of protection of the cardiovascular system by acupuncture and research on the mechanism of analgesia by acupuncture are hotspots in experimental research on acupuncture abroad. Studies on the amelioration of inflammatory pain and psychological or psychiatric disorders by acupuncture also serve as hotspots in clinical research on acupuncture abroad.</p></div><div><h3>Conclusion</h3><p>Acupuncture research in China focuses on the treatment of internal diseases and visceral pain, while acupuncture research abroad concentrates on the alleviation and improvement of cancer and psychological and mental diseases. Treatment of internal diseases and mental diseases with acupuncture is the trend of future research.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 28-40"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49755804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential targets and mechanisms of Guarana in the treatment of Alzheimer’s disease based on network pharmacology","authors":"J.I.A.O. Zhilin,&nbsp;G.A.O. Xuemei","doi":"10.1016/j.dcmed.2023.02.005","DOIUrl":"https://doi.org/10.1016/j.dcmed.2023.02.005","url":null,"abstract":"<div><h3>Objective</h3><p>To dig the main active components and predict potential mechanisms of Guarana in the treatment of Alzheimer’s disease (AD) by network pharmacology method and molecular docking.</p></div><div><h3>Methods</h3><p>By digging into papers relating to this topic, chemical components in Guarana were obtained and used for drug-likeness analysis. Databases including HERB and Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) were used to obtain potential targets that the active components in Guarana might have effects on, and to find out diseases in association with the potential targets. Other databases such as GeneCards, a human gene database, and DisGeNET were used to identify the genes relating to AD, and a Wayne diagram was drawn to get the intersected targets in Guarana and AD. Subsequently, CytoScape software was adopted for the construction of a Guarana-intersected targets-AD map. After that, the intersected targets were uploaded to the Search Tool for the Retrieval of Interaction Gene/Proteins (STRING) database to acquire a protein-protein interaction (PPI) network diagram. Then, the key target proteins were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). In terms of molecular docking verification, AutoDock software was used to verify whether the crucial active compounds of Guarana’s components could bind to the key targets.</p></div><div><h3>Results</h3><p>A total of 140 potential targets for Guarana to treat AD were obtained. The results of PPI network analysis showed that interleukin 6 (IL-6), tumor necrosis factor (TNF), insulin (INS), mitogen-activated protein kinase 3 (MAPK3), transcription factor (JUN), cell tumor antigen p53 (TP53), caspase3 (CASP3), protein c-Fos (FOS), catalase (CAT), and Catenin beta-1 (CTNNB1) might be the key targets of Guarana in the treatment of AD. It was found by GO and KEGG analyses that the mechanism of Guarana in the treatment of AD might be the bindings between Guarana compounds and protease outside the cell membranes. The molecular docking results showed that the small molecules of various components in Guarana binding to target proteins such as TNF, IL-6, MAPK3, and FOS needed relatively less energy.</p></div><div><h3>Conclusion</h3><p>The treatment of AD with Guarana involves the participation of multiple targets, among which IL-6, TNF, and MAPK3 might be the key ones. These key targets might take effects through the biological process in their bindings to <em>β</em>-amyloid and involving signaling pathways in cancer. Hopefully, our research could offer some scientific foundations as well as references for in-depth studies on the treatment of AD with Guarana.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"6 1","pages":"Pages 55-66"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49753304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}