Digital Chinese Medicine最新文献

{"title":"Acupuncture and Related Therapies for Hyperlipidemia: A Network Meta-Analysis","authors":"Wang Xue-Song ,&nbsp;Wang Yue-Shen ,&nbsp;Li Jia-Jia ,&nbsp;Yu Chao-Chao ,&nbsp;Wu Miao ,&nbsp;Kong Li-Hong","doi":"10.1016/j.dcmed.2020.12.002","DOIUrl":"https://doi.org/10.1016/j.dcmed.2020.12.002","url":null,"abstract":"<div><h3>Objective</h3><p>To compare and rank the clinical effects of different acupuncture and related therapies on hyperlipidemia patients.</p></div><div><h3>Methods</h3><p>We used network meta-analysis (NMA) to evaluate direct and indirect effects in studies of acupuncture and related therapies for hyperlipidemia. Databases PubMed, EMBASE, Cochrane Library, the China Biology Medicine (CBM), the China National Knowledge Infrastructure (CNKI), Wanfang Data and the Chinese Scientific Journal Database (VIP) were searched to collect randomized controlled trials (RCTs) of acupuncture and related therapies in the treatment of hyperlipidemia. The data were analyzed using Stata 15.0 and WinBUGS 1.4.3 software after two researchers independently screened the literature, extracted the data, and assessed the risk of bias in the included studies.</p></div><div><h3>Results</h3><p>We analyzed a total of 36 eligible studies that included 3 124 patients, involving 12 types of acupuncture and related therapies and comprehensive therapies. The results of the NMA showed that: for the total cholesterol (TC), acupoint catgut embedding (ACE), simple acupuncture (ACU), acupoint injection (AI), electroacupuncture (EA), western medicine of statins (WM), and combination of acupuncture and related therapies (combined therapies) were all more effective than placebo (<em>P</em> &lt; 0.05). For triacylglycerol (TG), ACU, EA, warming acupunc-ture (WA), WM and combined therapies were better than placebo (<em>P</em> &lt; 0.05), while WA was better than Chinese herb (CH) (<em>P</em> &lt; 0.05). For low-density lipoprotein cholesterol (LDL-C), combined therapies were more effective than lifestyle modification (LM) (<em>P</em> &lt; 0.05). For high-density lipoprotein choles-terol (HDL-C), auricular acupoint stimulation (AAS), ACE, ACU, AI, CH, EA, LM, moxibustion (MOX), WM, combined therapies and placebo were all worse than WA (<em>P</em> &lt; 0.05), while WM and combined therapies were better than ACU (<em>P</em> &lt; 0.05). Combined ranking results suggest that ACU and combined therapies may be the optimal intervention.</p></div><div><h3>Conclusions</h3><p>The efficacy of all kinds of acupuncture-related therapies in patients with hyperlipidemia is better than lifestyle changes. However, for different outcome indicators, all kinds of acupuncture-related therapies have their advantages and disadvantages, and comprehensive ranking results suggest that ACU and combined therapies may be the optimal intervention.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136473809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Biological Modeling: A Novel Approach to Interpret the Traditional Chinese Medicine Theory of Exterior-Interior Correlation Between the Lung and Large Intestine","authors":"Chen Wen-Lu ,&nbsp;Hong Jia-Na ,&nbsp;Zhang Xin-Ning ,&nbsp;Emmanuel Ibarra-Estrada ,&nbsp;Wan Li-Sheng ,&nbsp;Li Sha-Sha ,&nbsp;Yan Shi-Kai ,&nbsp;Xiao Xue","doi":"10.1016/j.dcmed.2020.12.004","DOIUrl":"10.1016/j.dcmed.2020.12.004","url":null,"abstract":"<div><h3>Objective</h3><p>To study the common pathogenesis of pneumonia and colitis using modern biological network analysis tools, and to explore the theory that the lung and large intestine are exteriorly and interiorly related.</p></div><div><h3>Methods</h3><p>The relevant target genes (hereinafter, “targets”) of pneumonia and colitis were separately queried on the GeneCards database. The main targets of the two diseases were then screened out according to their correlation scores and intersected to obtain those common to the two diseases. Metascape was used to analyze the main and common targets identified, and the Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to enrich and analyze the common targets. Cytoscape 3.7.2 software was used to build the network diagram.</p></div><div><h3>Results</h3><p>In total, 54 targets, such as TNF, IL-10, IL-6, IL-2, IL-4, TLR4, TLR2, CXCL8, IL-17A and IFNG, etc., are common to pneumonia and colitis, which are mainly enriched in these processes such as cytokine–cytokine receptor interaction, the T-cell receptor signaling pathway, the Toll-like receptor signaling pathway and the Jak-STAT signaling pathway. The Metascape modular analysis identified 11 modules for pneumonia, six modules for colitis, and two modules for the common targets.</p></div><div><h3>Conclusions</h3><p>Pneumonia and colitis have the same pathogenic targets and mechanisms of action and finally interact with each other through inflammatory reactions and immune responses. This provides a probable molecular mechanism that explains the theory that the lung and large intestine are exteriorly and interiorly related.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.12.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"108307346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Mechanism of Total Flavonoids of Chrysanthemum and Medicated Serum on Castrated Dry Eye Animal and Cell Models","authors":"Shi Jian ,&nbsp;Chen Li-Hao ,&nbsp;Liu Qian-Hong ,&nbsp;Peng Jun ,&nbsp;Tang Yu ,&nbsp;Yao Xiao-Lei ,&nbsp;Liu Zu-Guo","doi":"10.1016/j.dcmed.2020.12.007","DOIUrl":"10.1016/j.dcmed.2020.12.007","url":null,"abstract":"<div><h3>Objective</h3><p>To observe the effects of total flavonoids of chrysanthemum and medicated serum on the expression of related proteins in the lacrimal tissue and dry-eye cell models of male rabbits with dry eye caused by castration.</p></div><div><h3>Methods</h3><p>(1) 150 male Japanese rabbits were randomly divided into five groups, with 30 rabbits in each group: normal control group (group A), sham group (group B), model group (group C), androgen control group (group D) and total flavonoids of chrysanthemum treatment group (group E). The androgen deficiency dry-eye model was established by bilateral castration in groups C, D and E. Normal saline was administered to groups A, B and C by gavage; androgen (testosterone propionate) was injected into muscle in group D; and group E was given total flavonoids of chrysanthemum by gavage. All white rabbits were tested the Schirmer I test (SIT) and tear break-up time (BUT). After euthanasia, tear gland tissue was harvested so that we could observe pathological changes in the expression of related inflammatory factors in the lacrimal gland tissue. The expression of interleukin-1<em>β</em> (IL-1<em>β</em>), tumor necrosis factor-<em>α</em> (TNF-<em>α</em>) and transforming growth factor-<em>β</em>1 (TGF-<em>β</em>1) was detected in the lacrimal gland tissue by immunohistochemistry. Reverse transcription PCR was used to quantitatively detect expression of TGF-<em>β</em>1 mRNA. (2) Male Wistar rat lacrimal epithelial cells were used to establish a model of eye stem cell apoptosis caused by androgen levels. The blank control group was set up without androgen culture, the control group with androgen culture, and the total flavonoids of chrysanthemum group without androgen. The MTT method was used to determine the optimal intervention dosage of drug-containing plasma. Western blot and QPCR were used to detect the expression of AR mRNA, NF-<em>κ</em>B phosphorylated protein and TGF-<em>β</em>1 in lacrimal epithelial cells, and the androgen-like effect of total flavonoids of chrysanthemum was observed.</p></div><div><h3>Results</h3><p>(1) Immunohistochemistry showed that groups A, B, D and E had significantly lower expression of IL-1<em>β</em> and TNF-<em>α</em> than group C (<em>P</em> &lt; 0.05); among these, group E had slightly higher expression than group D (<em>P</em> &gt; 0.05). RT-PCR results showed that the relative expression of TGF-<em>β</em>1 mRNA in groups A, B, D and E was significantly higher than in group C (<em>P</em> &lt; 0.05), and the relative expression of TGF-<em>β</em>1 mRNA in groups D and E was higher than that in groups A and B (<em>P</em> &lt; 0.05). (2) Using the MTT method, the final concentration of interfering cells was calculated to be 13.2%. The expression of AR protein, NF-<em>κ</em>B and TGF-<em>β</em>1 in the chrysanthemum flavonoid plasma intervention and testosterone propionate intervention groups was enhanced, and there were significant differences relative to the b","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.12.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"104094442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of A Folic Acid-Modified Arsenic Trioxide Prodrug Liposome and Assessment of its Anti-Hepatocellular Carcinoma Activity","authors":"Zhu Jing-Jing ,&nbsp;Chen Xiao-Jie ,&nbsp;Yao Wen-Dong ,&nbsp;Wei Ying-Hui ,&nbsp;Zheng Hang-Sheng ,&nbsp;Zheng Hong-Yue ,&nbsp;Zhu Zhi-Hong ,&nbsp;Wang Bin-Hui ,&nbsp;Li Fan-Zhu","doi":"10.1016/j.dcmed.2020.12.005","DOIUrl":"10.1016/j.dcmed.2020.12.005","url":null,"abstract":"<div><h3>Objective</h3><p>To reduce the toxicity and side effects of arsenic trioxide (ATO) and provide a new approach for the treatment of primary liver cancer, a folic acid-modified calcium arsenite liposomal “target-controlled” drug delivery system (FA-LP-CaAs) was fabricated using the reverse microemulsion method.</p></div><div><h3>Methods</h3><p>A Malvern particle size analyzer and a transmission electron microscope were employed to determine the particle size, distribution, zeta potential and morphology of FA-LP-CaAs. Further, inductively coupled plasma emission spectrometry was employed to determine the drug loading capacity, entrapment efficiency, and <em>in vitro</em> release behavior of FA-LP-CaAs. To determine its toxicity in human hepatoma cells (HepG2) and human normal hepatocytes (LO2) and its effect on HepG2 cell cycle and apoptosis, the MTT method was used. Laser confocal and flow cytometry were also employed to determine the uptake of FA-LP-CaAs by cells. After establishing a mouse liver cancer model, the <em>in vivo</em> distribution of the drug included in the formulation was investigated using <em>in vivo</em> fluorescence. To evaluate the liver cancer targeting and anti-tumor effects of FA-LP-CaAs <em>in vivo</em>, the distribution of ATO in tissues and changes in tumor volume and body weight after liposomal administration were investigated using hematoxylin-eosin (HE)-stained tumor sections.</p></div><div><h3>Results</h3><p>The particle size, zeta potential and PDI of FA-LP-CaAs were (122.67 ± 2.18) nm, (12.81 ± 0.75) mV and 0.22 ± 0.01, respectively, while its drug loading capacity was 18.49% ± 1.14%. <em>In vitro</em> experimental results revealed that FA-LP-CaAs had a strong killing effect on HepG2 cells. Further, the cell uptake capacity of this formulation was found to improve. Based on <em>in vivo</em> assessments, FA-LP-CaAs could significantly increase the distribution of ATO in tumor sites and inhibit tumor growth.</p></div><div><h3>Conclusions</h3><p>Herein, an FA-LP-CaAs formulation was successfully fabricated. This liposomal drug delivery system had a round appearance, uniform particle size, good polydispersity coeffi-cient, evident “core-shell” structure, high drug loading capacity and pH response, tumor targeted drug delivery and sustained drug release. These findings support further research and the application of ATO as an anti-liver cancer prodrug and provide a new method for the treatment of liver cancer.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.12.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92938613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Potential Flavonoid Inhibitors of the SARS-CoV-2 Main Protease 6YNQ: A Molecular Docking Study","authors":"Sumit Arora ,&nbsp;Govind Lohiya ,&nbsp;Keshav Moharir ,&nbsp;Sapan Shah ,&nbsp;Subhash Yende","doi":"10.1016/j.dcmed.2020.12.003","DOIUrl":"10.1016/j.dcmed.2020.12.003","url":null,"abstract":"<div><h3>Objective</h3><p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent for coronavirus disease 2019 (COVID-19), is responsible for the recent global pandemic. As there are no effective drugs or vaccines available for SARS-CoV-2, we investigated the potential of flavonoids against SARS-CoV-2 main protease 6YNQ.</p></div><div><h3>Methods</h3><p><em>In silico</em> molecular simulation study against SARS-CoV-2 main protease 6YNQ.</p></div><div><h3>Results</h3><p>Among the 21 selected flavonoids, rutin demonstrated the highest binding energy (− 8.7 kcal/mol) and displayed perfect binding with the catalytic sites.</p></div><div><h3>Conclusions</h3><p>Our study demonstrates the inhibitory potential of flavonoids against SARS-CoV-2 main protease 6YNQ. These computational simulation studies support the hypothesis that flavonoids might be helpful for the treatment of COVID-19.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82790900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yi Qi Jie Du Formula and Salinomycin Combination Treatment Mediates Nasopharyngeal Carcinoma Stem Cell Proliferation, Migration and Apoptosis via CD44/Ras Signaling Pathway","authors":"He Lan ,&nbsp;Zhou Fang-Liang ,&nbsp;Zou Pan ,&nbsp;Wang Xian-Wen ,&nbsp;Jiang Yi-Lan ,&nbsp;He Ying-Chun ,&nbsp;Liao Duan-Fang ,&nbsp;Cao De-Liang","doi":"10.1016/j.dcmed.2020.12.008","DOIUrl":"10.1016/j.dcmed.2020.12.008","url":null,"abstract":"<div><h3>Objective</h3><p>To assess the effects of Yi Qi Jie Du Formula (YQJDF) combined with salinomycin (SAL) on nasopharyngeal carcinoma stem cells (NPC-SCs) and investigate the underlying molecular mechanisms.</p></div><div><h3>Methods</h3><p>Cell counting methods, the CCK-8 assay, transwell migration assay and JC-1 staining, were used to observe the effects of the combination on the proliferation, migration and apoptosis of NPC stem cells, respectively. Western blot was used to detect the levels of protein in NPC-SCs.</p></div><div><h3>Results</h3><p>YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPC-SC apoptosis. Mechanistically, YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins, including cleaved Caspase-3, cleaved Caspase-7 and cleaved Caspase-9. Moreover, YQJDF combined with SAL synergistically decreased the levels of CD44, p-c-Src, Ras, p-PKCδ, p-MEK, p-c-Raf, p-ERK1/2 and p-AKT proteins.</p></div><div><h3>Conclusions</h3><p>The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis, which may be closely related to the downregulation of the CD44/Ras signaling pathway.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.12.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"98972383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant, Antimicrobial and Wound Healing Potential of Helicteres isora Linn. Leaf Extracts","authors":"Renuka Mahajan,&nbsp;Prakash Itankar","doi":"10.1016/j.dcmed.2020.09.005","DOIUrl":"https://doi.org/10.1016/j.dcmed.2020.09.005","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the antioxidant, antimicrobial and wound healing potential of <em>Helicteres isora</em> Linn. leaf extracts.</p></div><div><h3>Methods</h3><p>The petroleum ether, chloroform, acetone, ethanol and hydroalcoholic extracts of leaves were screened for phytochemicals. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO) radical scavenging tests and reducing power assays were performed to measure antioxidant activity; disc diffusion methods were used to evaluate antimicrobial potential. Wound healing activity was evaluated by incision and excision wound rat models.</p></div><div><h3>Results</h3><p>The extracts contained mainly sterols and flavonoids. The hydroalcoholic extract showed remarkable antioxidant and antimicrobial potential and significant (<em>P</em> &lt; 0.05) wound healing activity.</p></div><div><h3>Conclusions</h3><p>The identified activities of the hydroalcoholic extract may be attributable to its constituent phytochemicals.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.09.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91779134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation Production of Ganoderma lucidum by Bacillus subtilis Ameliorated Ceftriaxone-induced Intestinal Dysbiosis and Improved Intestinal Mucosal Barrier Function in Mice","authors":"Liu He,&nbsp;Fan Qing-Jie,&nbsp;Liu Yin-Hui,&nbsp;Li Ming,&nbsp;Yuan Jie-Li","doi":"10.1016/j.dcmed.2020.09.006","DOIUrl":"https://doi.org/10.1016/j.dcmed.2020.09.006","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to develop a type of <em>Ganoderma lucidum</em> (<em>G. lucidum</em>)-probiotic fermentation broth that can effectively improve the intestinal mucosal barrier function of mice with ceftriaxone-induced intestinal dysbiosis.</p></div><div><h3>Methods</h3><p>By means of absorbance of optical density (OD) value and phenol-concentrated sulfuric acid measurement of polysaccharide content, the probiotic species can grow on the medium of <em>G. lucidum</em> were screened out, and the concentration of the medium of <em>G. lucidum</em> was determined, and the fermentation broth was prepared for subsequent experiments. Thirty-two SPF grade male BALB/c mice were randomly divided into four groups (eight mice in each group), namely control group (CON), intestinal mucosal barrier damage model group (CS), fermentation broth intervention group (FT) and <em>G. lucidum</em> medium intervention group (GL), respectively. The intestinal dysregulation model was induced by intra-gastric administration of 0.2 mL ceftriaxone sodium (twice a day for seven consecutive days). From day 8, the FT group and GL group were gavage with 0.2 mL fermentation broth and <em>G. lucidum</em> medium, respectively. On day 15, all mice were sacrificed. To draw the weight curve and measure the cecal index; pathological examination of colon tissues with HE staining; serum levels of LPS, IL-10, TNF and IL-6 were detected by ELISA. Flow cytometry was used to analyze the changes of CD3 ⁺ T cells, CD4 ⁺ T cells, CD8 ⁺ T cells and macro- phages in spleen. 16S rRNA sequencing was performed to detect the intestinal microbiota structure of mice.</p></div><div><h3>Results</h3><p><em>Bacillus subtilis</em> can decompose and utilize <em>G. lucidum</em> fruiting body medium, and the suitable concentration of <em>G. lucidum</em> fruiting body medium is 33.2 mg/mL. The effect of <em>Bacillus subtilis</em>-<em>G. lucidum</em> fermentation broth on the damage of intestinal mucosal barrier caused by ceftriaxone sodium was reduced, the body weight of mice recovered and colon swellingimproved, colon histopathological injury was alleviated, inflammatory cell infiltration was alleviated, serum IL-10 increased significantly, LPS 、 TNF-<em>α</em> and IL-6 decreased significantly compared with model group, and the proportion of T cells and intestinal dysbiosis was improved.</p></div><div><h3>Conclusions</h3><p>The experimental results suggest that <em>Bacillus subtilis</em>-<em>G. lucidum</em> fermentation broth can effectively improve the intestinal barrier function damage, immune dysfunction and intestinal dysbiosis caused by antibiotic overdose, and has a certain regulatory effect on intestinal mucosal barrier function.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.09.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91779135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Network Pharmacology Study on the Effects of Ma Xing Shi Gan Decoction on Influenza","authors":"Long Xi ,&nbsp;Liu Le-Ping ,&nbsp;Xu Xin-Yi ,&nbsp;L.I. Ling ,&nbsp;Zhang Guo-Min","doi":"10.1016/j.dcmed.2020.09.003","DOIUrl":"https://doi.org/10.1016/j.dcmed.2020.09.003","url":null,"abstract":"<div><h3>Objective</h3><p>Pharmacological methods were used to screen targets and signaling pathways of Ma Xing Shi Gan Decoction (MXSGD) during influenza treatments, and mechanisms underlying anti-influenza effects were elucidated.</p></div><div><h3>Methods</h3><p>The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and relevant literature were searched under predefined conditions to identify the main compounds and their targets. Interactions between the target proteins were predicted using the STRING database. Gene Ontology (GO) functional enrichment analyses and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed on the core targets involved in the influenza protein-protein interaction (PPI) network, using WebGestalt and the reactome database. iGEMDOCK was used for molecular docking of receptors and ligands to produce docking scores, and the results were visualized using Autodock and PyMOL.</p></div><div><h3>Results</h3><p>In total, 126 major compounds and their respective targets were screened. 355 influenza target proteins and 1 221 influenza protein interactions were predicted using the STRING database. Influenza-related signaling pathways were strongly enriched in pharmacodynamic targets of MXSGD such as cytokine signaling in immune system and signaling by inter-leukin. The main biological process was response to the stimulates. Molecular docking results showed that RELA-Licochalcone A docking elicited by MXSGD, was superior to that of other target proteins and active compounds, suggesting that the docking site is also the main effector site of MXSGD during influenza treatments. <strong>Conclusions</strong> The results showed that MXSGD exerts anti-influenza effects by interfering with virus adsorption, inhibiting virus proliferation, influencing immune functions and protecting host cells, which may prevent inflammation-induced tissue damage.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.09.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91779131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Metabolomics Study of the Volatile Oil from Prunella vulgaris L. on Pelvic Inflammatory Disease","authors":"Deng Jing,&nbsp;Su Qian,&nbsp;Lin Xiu-Lian,&nbsp;Lin Yan,&nbsp;Li Ya-Mei,&nbsp;Lin Li-Mei,&nbsp;Liao Duan-Fang,&nbsp;Xia Bo-Hou","doi":"10.1016/j.dcmed.2020.09.007","DOIUrl":"https://doi.org/10.1016/j.dcmed.2020.09.007","url":null,"abstract":"<div><h3>Objective</h3><p>Pelvic inflammatory disease (PID) is one of the most common gynaecological diseases. Here, this thesis aims to investigate the therapeutic effects of <em>Prunella vulgaris</em> L. oil on the PID by using metabolomics based on gas chromatography- mass spectrometry (GC-MS) to address this challenge.</p></div><div><h3>Methods</h3><p>First, measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model. Furthermore, the volatile oil from <em>Prunella vulgaris</em> L. was administered to treat PID rats. Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample. The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.</p></div><div><h3>Results</h3><p>Some metabolites, such as acetic acid, succinic acid, glyceric acid, (R*,S*)-3,4-dihydroxybutanoic acid, 3-hydroxy- phenylacetic acid, D-ribose and myo-inositol showed a higher contribution in the classification model; thus, they can be considered as potential biomarkers. Furthermore, the therapeutic effect of the volatile oil extracted from <em>Prunella vulgaris</em> L. could also be visualized using GC-MS-based metabolomics.</p></div><div><h3>Conclusions</h3><p>The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dcmed.2020.09.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91779133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}