Digital Chinese Medicine最新文献

{"title":"Application of Nuc-mtDNA and DNA methylation analysis in life science and medical studies","authors":"Zhang Jia , L.I. Kai , Liao Duanfang","doi":"10.1016/j.dcmed.2022.10.011","DOIUrl":"10.1016/j.dcmed.2022.10.011","url":null,"abstract":"","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 233-235"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000581/pdfft?md5=30e84bab4b7e9664a07dc10bfa3320a5&pid=1-s2.0-S2589377722000581-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77327456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual analysis of research hotspots and trends in the treatment of immune thrombocytopenia with traditional Chinese medicine","authors":"Gao Yao ,&nbsp;Wu Zhenhui ,&nbsp;Wu Min ,&nbsp;Abid Naeem ,&nbsp;Li Weizhizi ,&nbsp;Zhang Daling ,&nbsp;Liu Fan ,&nbsp;Zeng Yingjian","doi":"10.1016/j.dcmed.2022.10.009","DOIUrl":"10.1016/j.dcmed.2022.10.009","url":null,"abstract":"<div><h3>Objective</h3><p>Through bibliometrics and visual analysis of the related studies on traditional Chinese medicine (TCM) treatment of immune thrombocytopenia (ITP), this study aims to sort out the overall research progress, hotspots, and trends in this field, and provide reference for further research in ITP.</p></div><div><h3>Methods</h3><p>The articles on ITP treated by TCM were retrieved from China National Knowledge Infrastructure (CNKI), Wanfang Database, China Science and Technology Journal Database (VIP), Web of Science Core Collection (WOSCC), and PubMed. The retrieval time was from the establishment of the databases to July 31, 2022. VOSviewer, CiteSpace, Carrot2, and NoteExpress were used for data analysis of the articles in terms of their quantities, types, and journals, and for visualization of research hotspots, authors, institutions, and keywords.</p></div><div><h3>Results</h3><p>1 493 Chinese articles and 40 English articles were included. The articles in Chinese mainly focus on clinical trial research and clinical experience summary, while the English articles mainly focus on clinical trial research and animal research. The Chinese articles were published in 317 Chinese journals, while English articles were published in 29 English journals. Research hotspots include the clinical syndrome differentiation of ITP, the therapeutic effect of TCM compounds on ITP, and the mechanism of ITP treatment. Keyword analysis shows that there are many research achievements in integrated traditional Chinese and western medicine treatment, clinical research, famous doctors’ experience, TCM treatment, cellular immunity, and humoral immunity. The authors with the most articles in Chinese and English are Professor CHEN Xinyi and Professor MA Rou, respectively, and the research institutions with the most articles are Dongzhimen Hospital of Beijing University of Chinese Medicine and Xiyuan Hospital of China Academy of Chinese Medical Sciences. Chinese herbs often used to treat ITP clinically include Xianhecao (Agrimoniae Herba), Nvzhenzi (Ligustri Lucidi Fructus), Mohanlian (Ecliptae Herba), Zhongjiefeng (Sarcandrae Herba), etc., and the prescription usually used to treat ITP include Guipi Decoction (归脾汤), Xijiao Dihuang Decoction (犀角地黄汤), Bazhen Decoction (八珍汤), Erzhi Pill (二至丸), and Xiaochaihu Decoction (小柴胡汤). The main development trends toward retrospective study, TCM treatment mechanism, and data mining.</p></div><div><h3>Conclusion</h3><p>The research on TCM treatment of ITP has progressed steadily, but in-depth studies and close cooperation between research institutions are necessary for the modernization of TCM in treating ITP.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 326-339"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000568/pdfft?md5=26ec3e3a10a8d0bb24ce4c96c76bbd17&pid=1-s2.0-S2589377722000568-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88885097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary silymarin ameliorating reproductive and lactation performance of sows via regulating body antioxidant and metabolism","authors":"Wei Liangkai ,&nbsp;Hou Gaifeng ,&nbsp;Long Cimin ,&nbsp;Chen Fengming ,&nbsp;Bai Xinliang ,&nbsp;Li Rui ,&nbsp;Yin Yulong","doi":"10.1016/j.dcmed.2022.10.005","DOIUrl":"10.1016/j.dcmed.2022.10.005","url":null,"abstract":"<div><h3>Objective</h3><p>The experiment was conducted to explore the effects of silymarin on reproductive and lactation performance, serum antioxidants, and body metabolism of sows.</p></div><div><h3>Methods</h3><p>Sixty pregnant sows (85 d, Large × Landrace) with similar genetic background, body condition, and parity were randomly divided into three groups, and each group has 20 individually housed sows. The sows in the control group (CG) were fed with basal diet, and those in the experimental group A (EGA) and B (EGB) were fed with basal diet containing 250 and 500 mg/kg silymarin, respectively. The experiment lasted 46 days from day 85 of gestation to the end of lactation (weaning on day 17 ).The milk composition and serum biochemical parameters were determined by a milk composition analyzer and a blood biochemical analyzer, respectively. Serum antioxidant indexes and plasma hormone levels were measured using the biochemical kits. The gas chromatograph was applied to detect the fecal short chain fatty acids.</p></div><div><h3>Results</h3><p>Compared with the CG, the total feed intake (TFI) and average daily feed intake (ADFI) were significantly increased (<em>P</em> &lt; 0.01), and the urea content in regular milk tended to increase (<em>P</em> = 0.095) in the EG. The serum malondialdehyde (MDA) contents were decreased on day 90 of gestation and the day of farrowing (<em>P</em> &lt; 0.01), and the serum contents of total antioxidant capacity (T-AOC) tended to increase on day 17 of lactation (<em>P</em> = 0.099) compared with the CG. Compared with the CG, the serum triglyceride (TG) concentrations in the EG tended to increase on day 90 of gestation (<em>P</em> = 0.062), and the content of serum total protein (TP) and albumin (ALB) reduced on day 17 of lactation (<em>P</em> &lt; 0.01). Compared with the CG, plasm D-lactic acid content was decreased (<em>P</em> &lt; 0.05), and the plasma prostaglandin (PG) level tended to increase (<em>P</em> = 0.088) in the EG on the day of farrowing and day 17 of lactation. Compared with the CG, fecal isobutyric acid concentration in the EG significantly decreased on the day of farrowing ( <em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>Adding 250 – 500 mg/kg silymarin to diets from the late gestation to the end of lactation could improve reproductive and lactation performance of sows via the regulation of nutrient metabolism and serum antioxidant.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 286-294"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000520/pdfft?md5=f9c3c5cce0e23351bb103e327c36a23b&pid=1-s2.0-S2589377722000520-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85699500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compound Chaijin Jieyu Tablets ameliorating insomnia complicated with depression by improving synaptic plasticity via regulating orexin A, melatonin, and acetylcholine contents","authors":"H.A.N. Yuanshan ,&nbsp;L.I.A.O. Xiaolin ,&nbsp;R.E.N. Tingting ,&nbsp;W.A.N.G. Yeqing ,&nbsp;L.I. Zirong ,&nbsp;Z.O.U. Manshu ,&nbsp;W.A.N.G. Yuhong","doi":"10.1016/j.dcmed.2022.10.007","DOIUrl":"10.1016/j.dcmed.2022.10.007","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the efficacy and mechanism of action of Compound Chaijin Jieyu Tablets (复方柴金解郁片, CCJJYT) in rats with insomnia complicated with depression.</p></div><div><h3>Methods</h3><p>Seventy-two Sprague-Dawley rats were randomly assigned into eight groups: the control, chronic unpredictable mild stress (CUMS), sleep deprivation (SD), CUMS + SD, positive drug (venlafaxine hydrochloride + diazepam), CCJJYT high-dose (CCJJYT˗2×), medium-dose (CCJJYT˗1×), and low-dose (CCJJYT˗0.5×) groups, with nine rats in each group. Depression-like behavior was evaluated by body weight, food intake, and behavioral tests such as the sucrose preference test (SPT), open field test (OFT), forced swimming test (FST), and pentobarbital-induced sleep test (PST). Hematoxylin-eosin (HE) staining and Golgi-Cox staining were used to observe changes in pathological tissue and synaptic morphology, respectively. Enzyme-linked immunosorbent assay (ELISA) was used to detect the contents of orexin-A and acetylcholine. The expression levels of orexin receptor 1 (OXR1), melatonin receptor 1 (MT1A), melatonin receptor 2 (MT1B), acetylcholinesterase (AChE), and choline acetyltransferase (ChAT) were detected by immunohistochemistry and Western blot.</p></div><div><h3>Results</h3><p>In the present study, rats in the model group showed significant behavioral changes as well as a reduction in hippocampal dendritic branch length and synaptic number, along with increasing the content of orexin A and acetylcholine (<em>P</em>&lt; 0.05), and altered expression levels of OX1R, MT1A, MT1B, ChAT, and AChE in the hippocampus and prefrontal cortex after modeling (<em>P</em> &lt; 0.05). CCJJYT can improve depressive insomnia behavior and synaptic plasticity of rats (<em>P</em> &lt; 0.05), which is similar to that of the positive drug group. It can also decrease the content of orexin A and acetylcholine, and reduce the expression levels of OXR1 and ChAT in hippocampus and prefrontal cortex (<em>P</em> &lt; 0.05), and increase the expression levels of MT1A, MT1B, and AChE proteins (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>CCJJYT has good antidepressant and insomnia effects, probably through the regu-lation of orexin-A, melatonin, and acetylcholine content in hippocampus and prefrontal cortex of rats, improving synaptic plasticity and thus exerting antidepressant and insomnia effects.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 305-316"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000544/pdfft?md5=fc28a738cd59e1136d5aed602a693902&pid=1-s2.0-S2589377722000544-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88219253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Living fossils unearthed by blasting human chromosomes with Neanderthal mtDNA","authors":"Jia Zhang ,&nbsp;Cuilan Zhou ,&nbsp;Li Xiao ,&nbsp;Qinhui Tuo ,&nbsp;Cuiying Peng ,&nbsp;Zifen Guo ,&nbsp;Duanfang Liao ,&nbsp;Kai Li","doi":"10.1016/j.dcmed.2022.10.012","DOIUrl":"10.1016/j.dcmed.2022.10.012","url":null,"abstract":"<div><p>The successful retrieval of ancient mitochondrial DNA (mtDNA) from Neanderthals provides powerful experimental evidence that clarifies the arguments between the out-of-Africa and multiregional models of evolution. However, the lack of nuclear DNA from Neanderthal fossils and mtDNA of early modern human fossils dating back to approximately the same time in the Pleistocene constitutes a limitation that may compromise the significance of mtDNA phylogenetic analysis. In this report, we introduce a mitochromic analysis using Neanderthal mtDNA as a foreign transgene and humans as a naturally occurring transgenic species. Forty Neanderthal mtDNA retrievable nuclear fragments were identified by blasting human genome data with Neanderthal mtDNA. Five of the 40 fragments exhibited higher correlation with Neanderthal mtDNA than those with modern human mtDNA. Furthermore, these five nuclear fragments harbor Neanderthal mtDNA-unique haplotypes. Based on the 98%+ identity between Neanderthal and modern human mtDNA when compared by groups, we suggest that some of the modern human nuclear fragments retrieved using Neanderthal mtDNA may aid in decoding Neanderthal genetic information, and also may simultaneously demonstrate a close genetic evolutionary relationship between modern humans and Neanderthals.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 236-241"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000593/pdfft?md5=0e362df515cd1180c58ebd31b26f71d2&pid=1-s2.0-S2589377722000593-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72862177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application regularity of medicinal and dietary substances for patients during COVID-19 rehabilitation","authors":"L.U.O. Binfei ,&nbsp;H.E. Qingying ,&nbsp;Y.I. Xingqian ,&nbsp;L.I.U. Hongning ,&nbsp;Z.H.U. Weifeng ,&nbsp;W.U. Diyao ,&nbsp;Z.H.A.N.G. Anran ,&nbsp;Z.H.A.N.G. Xiaoping ,&nbsp;C.H.E.N. Xiaofan","doi":"10.1016/j.dcmed.2022.10.001","DOIUrl":"10.1016/j.dcmed.2022.10.001","url":null,"abstract":"<div><h3>Objective</h3><p>This study focused on the application regularity of medicinal and dietary substances (MDS) of traditional Chinese medicine (TCM) diet therapy during rehabilitation, in order to help patients with Corona Virus Disease 2019 (COVID-19) reduce sequelae and improve their life quality.</p></div><div><h3>Methods</h3><p>The official websites of the national and provincial health committees, the website of the National Administration of Traditional Chinese Medicine, the China BioMedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP), and Wanfang Database were used to search the keywords, such as “coronavirus” “novel coronavirus pneumonia” “COVID-19” “protocol” “guideline” “consensus” and “rehabilitation period”. The search time was from the establishment of databases to July 31, 2022. The prevention and control protocols of various provinces and cities were manually supplemented and screened out. The information on the frequency, property, flavor, meridian tropism, and efficacy of MDS was collected for association rule analysis through the Apriori algorithm. Hierarchical cluster analysis was performed using the Euclidean distance and longest distance.</p></div><div><h3>Results</h3><p>A total of 18 protocols were screened out, including 56 lists of TCM diet therapy, and 47 kinds of MDS with a frequency of 132 times during the rehabilitation of COVID-19. Among them, six lists of diet therapy were collected from national websites, 26 from local government websites, and 24 from social and academic institution websites. The intended population can be divided into seven categories including normal recovery, lung-spleen Qi deficiency, deficiency of both Qi and Yin, spleen-stomach weakness, deficiency of Yang Qi, kidney Qi deficiency, and blood deficiency. Shanyao (Dioscoreae Rhizoma) and Lianzi (Nelumbinis Semen), followed by Dazao (Jujubae Fructus) were used most commonly in MDS, with mainly flat property, sweet flavor, and spleen and lung meridians in meridian tropism. Besides, deficiency-tonifying drugs were commonly used in MDS. Through association rule analysis, 12 groups of association MDS pairs were obtained. The pair of Yiyiren (Coicis Semen) and Chenpi (Citri Reticulatae Pericarpium) had the highest Lift value, and Yiyiren (Coicis Semen) was used most frequently in the MDS category for eliminating pathogenic factors. The results of complex network analysis showed that the core MDS were Yiyiren (Coicis Semen), Shanyao (Dioscoreae Rhizoma), Huangqi (Astragali Radix), Fuling (Poria), and Dazao (Jujubae Fructus). Three core categories were classified by cluster analysis, including the category of strengthening spleen, nourishing kidney, and grasping Qi, the category of removing phlegm, abating panting, and regulating Qi, and the category of strengthening the middle-energizer and reinforcing Qi.</p></div><div><h3>Conclusion</h3><p>Based on the TCM theory","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 242-252"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000489/pdfft?md5=41f613d7d19b207729768144c1943b36&pid=1-s2.0-S2589377722000489-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76547707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Qingguang’an Granules on mitochondrial autophagy of retinal ganglion cells in rats with chronic ocular hypertension","authors":"Tang Yu ,&nbsp;Zhu Bingyao ,&nbsp;Shi Jian ,&nbsp;Liu Qianhong ,&nbsp;Chen Lihao ,&nbsp;Peng Qinghua ,&nbsp;Peng Jun ,&nbsp;Yao Xiaolei","doi":"10.1016/j.dcmed.2022.10.006","DOIUrl":"10.1016/j.dcmed.2022.10.006","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the effect and underlying mechanism of Qingguang’an Granules (青光安颗粒剂, QGAG) on mitochondrial autophagy (mitophagy) of retinal ganglion cells (RGCs) in rats with chronic ocular hypertension (COH).</p></div><div><h3>Methods</h3><p>Sixty Sprague Dawley (SD) rats, half males and half females, were randomly assigned to three groups: the control, model, and QGAG (2.5 g/kg) groups, with 20 rats in each group. Rats’ model of COH was established by cauterizing episcleral veins in the model group and QGAG group. Three weeks after successful modeling, rats in the QGAG group were intragastrically administered with QGAG, while rats in the control group and the model group received an equal dose of normal saline. After three months of intragastric administration, intraocular pressure (IOP) of all rats was measured. The mitophagy was monitored by the immunofluorescence method, the mitochondrial membrane potential was measured using the JC-1 method, and the morphological changes of mitophagy in RGCs were observed by transmission electron microscopy. Meanwhile, rat RGCs were labeled using the fluorescent gold method, and RGCs density in each group was calculated. Moreover, RGCs apoptosis was observed by TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. Finally, the expression levels of Parkin, optineurin, microtubule-associated protein 1 light chain 3-II/microtubule-associated protein 1 light chain 3-I (LC3-II/LC3-I), recombinant lysosomal associated membrane protein 1 (LAMP1), and B-cell lymphoma-2 (Bcl-2) in RGCs were determined by Western blot assay. The corresponding mRNAs were detected through quantitative real-time polymerase chain reaction (qRT-PCR).</p></div><div><h3>Results</h3><p>The QGAG reduced IOP in COH rats, and inhibited mitophagy and apoptosis of RGCs (<em>P</em> &lt; 0.05). Besides, the QGAG significantly increased the expression levels of Parkin and Bcl-2 (<em>P</em> &lt; 0.05), and inhibited the expression levels of optineurin, LAMP1, and LC3-II/LC3-I (<em>P</em> &lt; 0.05) in RGCs of COH rats.</p></div><div><h3>Conclusion</h3><p>The QGAG can inhibit mitophagy in RGCs of COH rats and show a protective effect against optic nerve damage caused by glaucoma, which may be mediated through the mitophagy ubiquitination via the Parkin/PINK1-related pathway.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 295-304"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000532/pdfft?md5=9fc0c445d7e5ea09568df060c6b95e71&pid=1-s2.0-S2589377722000532-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75797775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of the efficacy and safety of Huanglian Wendan Decoction alone or combined with western medicine in treating insomnia caused by phlegm-heat internal disturbance","authors":"Y.A.N.G. Lei ,&nbsp;H.E. Liyun ,&nbsp;C.H.E.N. Xiao ,&nbsp;W.A.N.G. Kaiyue ,&nbsp;F.A.N.G. Yongjun ,&nbsp;L.I.A.N.G. Zhuang ,&nbsp;Y.A.N.G. Zhao","doi":"10.1016/j.dcmed.2022.10.010","DOIUrl":"10.1016/j.dcmed.2022.10.010","url":null,"abstract":"<div><h3>Objective</h3><p>To evaluate the efficacy and safety of Huanglian Wendan Decoction (黄连温胆汤, HLWDD) alone or combined with western medicine in treating insomnia caused by phlegm-heat internal disturbance in recent 10 years.</p></div><div><h3>Methods</h3><p>The randomized controlled trials of HLWDD alone or combined with western medicine in treating insomnia caused by phlegm-heat internal disturbance from January 1, 2012 to April 1, 2022 were searched in China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP), Wanfang Database, China BioMedical Literature Database (CBM), PubMed, Web of Science, Embase, and Cochrane Library databases. After being screening, the included literature was analyzed to evaluate the effective rate, Pittsburgh Sleep Quality Index (PSQI) score, traditional Chinese medicine (TCM) syndrome score, and adverse reactions of HLWDD on insomnia caused by phlegm-heat internal disturbance. The subgroup analyzed the effect of HLWDD after different treatment courses, and compared the therapeutic effects of HLWDD alone and HLWDD combined with western medicine.</p></div><div><h3>Results</h3><p>Twenty-seven randomized controlled trials were finally included, with a total of 2 395 patients. The results of the meta-analysis showed that the curative effect of HLWDD alone or combined with the western medicine group was better than that of the western medicine group [RR = 1.14, 95% CI (1.06, 1.22), <em>P</em> = 0.000]. The PSQI score [SMD = – 0.31, 95% CI (– 0.42, – 0.20), <em>P</em> = 0.000], TCM syndrome score [SMD = – 0.40, 95% CI (– 0.67, – 0.12), <em>P</em> = 0.005], and adverse reaction rate [RR = 0.21, 95% CI (0.15, 0.29), <em>P</em> = 0.000] of HLWDD alone or combined with western medicine group were significantly reduced compared with the western medicine group. The subgroup’s analysis showed that the curative effect of HLWDD alone or combined with western medicine group of 4 weeks treatment course was better than that of the western medicine group [RR = 1.14, 95% CI (1.03, 1.26), <em>P</em> &lt; 0.05]. The TCM syndrome score of HLWDD alone or combined with the western medicine group of 4 weeks treatment course decreased more obviously than that of the western medicine group [SMD = – 0.60, 95% CI (– 0.96, – 0.25), <em>P</em> &lt; 0.05]. There were no significant differences between HLWDD alone or combined with western medicine group and western medicine group with different treatment courses based on PSQI score and adverse reaction rate. Based on the effective rate, the comparison between the HLWDD alone group and the western medicine group [RR = 1.09, 95% CI (1.00, – 1.20) <em>P</em> &lt; 0.05], and between the HLWDD combined with western medicine group and the western medicine group [RR = 1.15, 95% CI (1.03, 1.29), <em>P</em> &lt; 0.05] was the same. PSQI score [SMD = – 0.44, 95% CI (– 0.59, – 0.30), <em>P</em> &lt; 0.05] and TCM syndrome score [SMD = – 1.10, 95% CI (– 1.59, – 0.61)","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 340-352"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S258937772200057X/pdfft?md5=b4037fcab80192ff0f895d592daddcb4&pid=1-s2.0-S258937772200057X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91103785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the phytoconstituents targeting TNF-α as potential lead compounds to treat inflammatory diseases: an in-silico approach","authors":"Sumit Arora (Assistant Professor) ,&nbsp;Pallavi Rushiya ,&nbsp;Kalpana Tirpude ,&nbsp;Nidhi Sapkal ,&nbsp;Subhash Yende ,&nbsp;Abhay Ittadwar ,&nbsp;Sapan Shah","doi":"10.1016/j.dcmed.2022.10.003","DOIUrl":"10.1016/j.dcmed.2022.10.003","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the anti-inflammatory phytoconstituents from various plant sources as tumour necrosis factor-<em>α</em> (TNF-<em>α</em>)-inhibitor, a mediator involved in the inflammatory disorder, by <em>in silico</em> molecular docking.</p></div><div><h3>Methods</h3><p>Based on previous findings, we performed the <em>in silico</em> assessment of anti-inflammatory phytoconstituents from different medicinal plants to understand their binding patterns against TNF-<em>α</em> (PDB ID: 6OP0) using AutoDock Vina. Molecular docking was performed by setting a grid box (25 × 25 × 25) Å centered at [– 12.817 × (– 1.618) × 19.009] Å with 0.375 Å of grid spacing. Furthermore, Discovery Studio Client 2020 program was utilized to assess two- and three-dimensional (2D and 3D) hydrogen-bond interactions concerning an amino acid of target and ligand. Physicochemical properties were reported using the Lipinski’s rule and SwissADME database to support the <em>in silico</em> findings. <strong>Results</strong> From the selected medicinal plants, more than 200 phytocompounds were screened against TNF-<em>α</em> protein with binding scores in the range of – 12.3 to – 2.5 kcal/mol. Amongst them, emodin, aloe-emodin, pongamol, purpuritenin, semiglabrin, ellagic acid, imperatorin, <em>α</em>-tocopherol, and octanorcucurbitacin A showed good binding affinity as – 10.6, – 10.0, – 10.5, – 10.1, – 11.2, – 10.3, – 10.1, – 10.1, and – 10.0 kcal/mol, respectively. Also, the absorption, distribution, metabolism, excretion, and toxicology (ADMET) profiles were well within acceptable limits.</p></div><div><h3>Conclusion</h3><p>Based on our preliminary findings, we conclude that the selected phytoconstituents have the potential to be good anti-inflammatory candidates by inhibiting the TNF-<em>α</em> target. These compounds can be further optimized and validated as new therapeutic components to develop more effective and safe anti-inflammatory drugs.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 264-275"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000507/pdfft?md5=bed53cdb18a06e3212cb381db71f90a5&pid=1-s2.0-S2589377722000507-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84494860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tongue image segmentation and tongue color classification based on deep learning","authors":"L.I.U. Wei,&nbsp;C.H.E.N. Jinming,&nbsp;L.I.U. Bo,&nbsp;H.U. Wei,&nbsp;W.U. Xingjin,&nbsp;Z.H.O.U. Hui","doi":"10.1016/j.dcmed.2022.10.002","DOIUrl":"10.1016/j.dcmed.2022.10.002","url":null,"abstract":"<div><h3>Objective</h3><p>To propose two novel methods based on deep learning for computer-aided tongue diagnosis, including tongue image segmentation and tongue color classification, improving their diagnostic accuracy.</p></div><div><h3>Methods</h3><p>LabelMe was used to label the tongue mask and Snake model to optimize the labeling results. A new dataset was constructed for tongue image segmentation. Tongue color was marked to build a classified dataset for network training. In this research, the Inception + Atrous Spatial Pyramid Pooling (ASPP) + UNet (IAUNet) method was proposed for tongue image segmentation, based on the existing UNet, Inception, and atrous convolution. Moreover, the Tongue Color Classification Net (TCCNet) was constructed with reference to ResNet, Inception, and Triple-Loss. Several important measurement indexes were selected to evaluate and compare the effects of the novel and existing methods for tongue segmentation and tongue color classification. IAUNet was compared with existing mainstream methods such as UNet and DeepLabV3+ for tongue segmentation. TCCNet for tongue color classification was compared with VGG16 and GoogLeNet.</p></div><div><h3>Results</h3><p>IAUNet can accurately segment the tongue from original images. The results showed that the Mean Intersection over Union (MIoU) of IAUNet reached 96.30%, and its Mean Pixel Accuracy (MPA), mean Average Precision (mAP), F1-Score, G-Score, and Area Under Curve (AUC) reached 97.86%, 99.18%, 96.71%, 96.82%, and 99.71%, respectively, suggesting IAUNet produced better segmentation than other methods, with fewer parameters. Triplet-Loss was applied in the proposed TCCNet to separate different embedded colors. The experiment yielded ideal results, with F1-Score and mAP of the TCCNet reached 88.86% and 93.49%, respectively.</p></div><div><h3>Conclusion</h3><p>IAUNet based on deep learning for tongue segmentation is better than traditional ones. IAUNet can not only produce ideal tongue segmentation, but have better effects than those of PSPNet, SegNet, UNet, and DeepLabV3+, the traditional networks. As for tongue color classification, the proposed network, TCCNet, had better F1-Score and mAP values as compared with other neural networks such as VGG16 and GoogLeNet.</p></div>","PeriodicalId":33578,"journal":{"name":"Digital Chinese Medicine","volume":"5 3","pages":"Pages 253-263"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589377722000490/pdfft?md5=e87638a6b039111b86fa36dba4769d4f&pid=1-s2.0-S2589377722000490-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90909801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}