Infection Prevention in Practice最新文献

{"title":"Contamination dynamics of personal protective equipment (PPE) by SARS-CoV-2 RNA in a makeshift hospital with COVID-19 positive occupants","authors":"Tingting Xia ,&nbsp;Shi Shi ,&nbsp;Jinyan Yang ,&nbsp;Dan Sun ,&nbsp;Jijiang Suo ,&nbsp;Huihui Kuang ,&nbsp;Nana Sun ,&nbsp;Hongyan Hu ,&nbsp;Jinhan Xiao ,&nbsp;Zhongqiang Yan","doi":"10.1016/j.infpip.2023.100309","DOIUrl":"10.1016/j.infpip.2023.100309","url":null,"abstract":"<div><h3>Background</h3><p>Personal protective equipment (PPE) helps protect healthcare workers (HCWs) from infection and prevents cross-contamination. Knowledge of the contamination dynamics of PPE during the management of COVID-19 patients in a makeshift hospital is limited.</p></div><div><h3>Aim</h3><p>To describe the rate of SARS-CoV-2 contamination in PPE and to assess the change of contamination at different time points.</p></div><div><h3>Methods</h3><p>HCWs were followed up for up to 4 hours with hourly collection of swab samples from PPE surfaces in a makeshift COVID-19 hospital setting. Swabs were tested using quantitative reverse transcription polymerase chain reaction (RT-qPCR) for SARS-CoV-2 RNA.</p></div><div><h3>Results</h3><p>SARS-CoV-2 was detected on 50.9% of the 1620 swabbed samples from 9 different sites of full-body PPE worn by HCWs. The proportion of sites contaminated with SARS-CoV-2 RNA varied from 10.6% to 95.6%. Viral RNA was most frequently detected from the sole of the outer foot cover (95.6%) and least frequently on the face shield (10.6%). The median Ct values among positive samples were 34.20 (IQR, 32.61–35.22) and 34.05 (IQR, 32.20–35.39) for ORF1ab and N genes, respectively. The highest rate of contamination with SARS-CoV-2 RNA for the PPE swab samples was found after 3 hours of use. The positive rate of outer surface of HEPA filters from air supply device was 82.1% during the full capacity period of the makeshift hospital.</p></div><div><h3>Conclusion</h3><p>A higher rate of contamination was identified at 3 hours after the entrance to the COVID-19 patient care area. Virus-containing aerosols were trapped in the HEPA filter of air supply equipment, representing a potential protective factor against infection to HCWs.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/09/3d/main.PMC10514403.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41104124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, microbiological characteristics and predictors of mortality in patients with carbapenemase-producing Enterobacterales bloodstream infections: a multicentre study","authors":"Vanesa Anton-Vazquez ,&nbsp;Terry John Evans ,&nbsp;Samitha Fernando ,&nbsp;Donald Somasunderam ,&nbsp;Kate David ,&nbsp;Mark Melzer ,&nbsp;Lois Hawkins ,&nbsp;Stephen Morris-Jones ,&nbsp;Mauricio Arias ,&nbsp;Borana Drazho ,&nbsp;Martino Dall’Antonia ,&nbsp;Timothy Planche","doi":"10.1016/j.infpip.2023.100298","DOIUrl":"10.1016/j.infpip.2023.100298","url":null,"abstract":"<div><h3>Objectives</h3><p>To investigate the clinical, microbiological characteristics and outcomes of patients with bloodstream infections (BSI) due to carbapenemase-producing Enterobacterales (CPE).</p></div><div><h3>Methods</h3><p>A multicentre retrospective observational study of patients with BSIs due to CPE admitted to six UK hospitals was conducted between 2011 and 2021. Multivariate analysis was used to identify factors predicting 30-day case fatality rate (CFR).</p></div><div><h3>Results</h3><p>There were 84 episodes of CPE-BSIs, 37 (44%) due to OXA-48, 35 (42%) to metallo-betalactamases (MBL) and 12 (14%) to KPC. 63% of patients were male with a median age of 64 years. Common organisms included <em>Klebsiella</em> spp. (61%), <em>Escherichia coli</em> (20%) and <em>Enterobacter</em> spp. (13%). Urinary devices were more often involved in OXA-48 BSIs (12/37; 32%) compared to infections caused by MBL and KPC (4/35; 11% and 1/12; 8%; <em>P</em> = 0.046). In contrast, central venous catheters were more frequently present in KPC-BSIs (10/12; 92%) compared with OXA-48 and MBL (11/37; 30% and 20/35; 57%; <em>P</em> = 0.002). Effective definitive antimicrobials were received by 72/84 (86%) patients, comprising monotherapy (32/72; 44%) or combination therapy (40/72; 56%). 30-day case fatality rate (CFR) was 38%. Sepsis or septic shock was associated with death [OR 3.81 (CI 1.19–12.14), <em>P</em> = 0.024].</p></div><div><h3>Conclusion</h3><p>Strategies targeting high-risk patients and adherence to infection prevention bundles for urinary devices and central venous catheters can reduce OXA-48 and KPC-BSIs. Early recognition and management of severe sepsis, prompt initiation of appropriate antimicrobial therapy and development of novel antimicrobials are crucial to mitigate the high CFR associated with CPE-BSIs<strong>.</strong></p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/98/main.PMC10393540.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9924420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of supervised implementation of an oral health care protocol on ventilator-associated pneumonia patients in intensive care units: a double-blind multicenter randomized controlled trial","authors":"Sharare Karimi ,&nbsp;Ensi Kolyaei ,&nbsp;Pooya Karimi ,&nbsp;Khaled Rahmani","doi":"10.1016/j.infpip.2023.100295","DOIUrl":"10.1016/j.infpip.2023.100295","url":null,"abstract":"<div><h3>Background</h3><p>The available scientific evidence suggests a significant association between oral bacteria and the incidence of ventilator-associated pneumonia (VAP).</p></div><div><h3>Aims</h3><p>The aim of this study was to determine the effectiveness of an oral health protocol in the prevention of ventilator-associated pneumonia.</p></div><div><h3>Methods</h3><p>In this multi-center RCT, conducted in the intensive care units of neurological patients in three general teaching hospitals of two provinces located in the west of the Iran, a consecutive sample of 200 intubated patients were initially recruited. Patients were randomly allocated to the intervention (received oral health care based on evidence-based oral health protocol) or control (routine oral health care) groups. Both groups received their prescribed treatment regimen for seven consecutive days. The percentage of VAP diagnoses as main outcome assessed using clinical pulmonary infection score (CPIS).</p></div><div><h3>Findings</h3><p>The rate of VAP in the intervention and control groups was 5% and 64%, respectively. The intervention reduced the risk of VAP by 97% and this difference was statistically significant (<em>P</em> &lt;0.001). The chance of VAP occurrence in patients with lower levels of consciousness in univariate and multivariate analysis was significantly higher (OR: 2.38; 95%CI: 1.11–5.26) <em>P</em> &lt;0.05).</p></div><div><h3>Conclusion</h3><p>The results of our study suggest that the use of a dynamic supervised oral health care guideline is more effective than the routinely used protocols in the intensive care units of hospitals.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2a/69/main.PMC10338200.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9881486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Operating room disinfection: operator-driven ultraviolet ‘C’ vs. chemical treatment","authors":"Marie-Claire Fickenscher ,&nbsp;Madeline Stewart ,&nbsp;Ryan Helber ,&nbsp;Edward J. Quilligan ,&nbsp;Arthur Kreitenberg ,&nbsp;Carlos A. Prietto ,&nbsp;Vance O. Gardner","doi":"10.1016/j.infpip.2023.100301","DOIUrl":"10.1016/j.infpip.2023.100301","url":null,"abstract":"<div><h3>Background</h3><p>In operating room (OR) surfaces, Nosocomial pathogens can persist on inanimate surfaces for long intervals and are highly resistant to traditional surface cleaning.</p></div><div><h3>Aim</h3><p>This study compares traditional chemical operating room terminal disinfection to a unique operator-driven device that emits germicidal UV light at short distance onto vertical and horizontal surfaces.</p></div><div><h3>Methods</h3><p>A randomized crossover analogous protocol assigned 40 end-of-day operating rooms into either group A (chemical then UVC treatments) or group B (UVC then chemical treatments). Initial Staphylococcal cultures were obtained prior to disinfection treatment, after the first treatment, and after the second treatment at 16 most commonly contaminated sites to represent overall room contamination. Success was defined as no growth and failure as 1 or more colony forming units. Thoroughness of chemical treatment vs UVC treatment was compared and used to determine if the second treatment was additive to the first treatment within each group.</p></div><div><h3>Findings</h3><p>The operator driven UVC device outperformed chemical treatment in reducing the number of contaminated sites in the OR by more than half (<em>P</em>&lt;0.001). Operator-driven UVC reduced contaminated sites after chemical treatment by nearly half (<em>P</em>&lt;0.001). In contrast, chemical treatment after operator-driven UVC did not significantly reduce the number of contaminated sites. The mean employee time of disinfection for chemical treatment was 49 minutes and for the operator-driven UVC emitter 7.9 minutes (<em>P</em>&lt;0.001).</p></div><div><h3>Conclusions</h3><p>This study demonstrates that addition of an operator-driven UVC emitter to OR rooms between cases could be helpful in overall decreasing the number of contaminated sites.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/30/main.PMC10412461.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10052317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inactivation kinetics of benzalkonium chloride and ethanol-based hand sanitizers against a betacoronavirus and an alphacoronavirus","authors":"Brandon L. Herdt ,&nbsp;Luisa A. Ikner","doi":"10.1016/j.infpip.2023.100293","DOIUrl":"10.1016/j.infpip.2023.100293","url":null,"abstract":"<div><h3>Background</h3><p>Hand hygiene is critical to lower the potential for the spread of SARS-CoV-2 and other infectious agents by direct contact. When running water and soap are not available for hand hygiene, ethanol-based hand sanitizers are currently the recommended standard of care [1–3]. Though recently published data showed comparable <em>in vitro</em> effectiveness of benzalkonium chloride (BAK)-based and ethanol-based hand sanitizers against SARS-CoV-2 virus, a paucity of peer-reviewed data on the effectiveness of these formulations against other types of infective coronaviruses remains. This work assessed human coronavirus HCoV-229E (genus <em>Alphacoronavirus</em>) concurrently with SARS-CoV-2, Isolate USA-WA1/2020 (genus <em>Betacoronavirus</em>) to fill this gap.</p></div><div><h3>Methods</h3><p>The test was conducted according to EN14476:2013-A2:2019 [EN14476] Quantitative Suspension Test for the Evaluation of Virucidal Activity in the Medical Area [4]. Two BAK-based hand sanitizers, five ethanol-based hand sanitizers, and an 80% ethanol reference formulation were tested for antiviral activity against SARS-CoV-2 and HCoV-229E at 15- and 30- second contact times.</p></div><div><h3>Results</h3><p>Both SARS-CoV-2 and HCoV-229E were reduced by greater than 4.00-log₁₀ within 15 seconds of contact. Virus decay constants (<em>k</em>) following first-order kinetics were similar for BAK and ethanol-based formulations against both test viruses. The SARS-CoV-2 results reported herein mirrored previous data reported by Herdt <em>et al.</em> (2021).</p></div><div><h3>Conclusion</h3><p>BAK and ethanol hand sanitizer formulations inactivate SARS-CoV-2 and HCoV-229E at similar rates. This data supports previously published effectiveness data for both chemistries and indicates that additional coronavirus strains and variants would demonstrate similar inactivation trends.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3c/e4/main.PMC10266983.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9738855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the COVID-19 pandemic on the incidence of healthcare-associated Clostridioides difficile infection in a tertiary healthcare facility in the Republic of Ireland","authors":"Saied Ali,&nbsp;Sinead McDermott","doi":"10.1016/j.infpip.2023.100300","DOIUrl":"https://doi.org/10.1016/j.infpip.2023.100300","url":null,"abstract":"<div><h3>Background</h3><p><em>Clostridioides difficile</em> is the foremost cause of nosocomial infectious diarrhoea and one of the most prevalent healthcare associated infections (HAIs).</p></div><div><h3>Aims</h3><p>To investigate the impact of the coronavirus disease 2019 (COVID-19) pandemic on the incidence of healthcare associated <em>C. difficile</em> infection (HA-CDI).</p></div><div><h3>Methods</h3><p>A retrospective study was conducted from January 2019–December 2022 inclusive at a tertiary University Hospital in Dublin, Ireland. The study period was divided into COVID-19 and non-COVID-19 periods determined in tangent with the then national incidences of COVID-19 and number of hospitalized patients with COVID-19. Analyses looked at quantity of testing performed, incidence rates and antimicrobial consumption. An independent samples t-test was used to determine significance between groups.</p></div><div><h3>Results</h3><p>Between COVID-19 and non-COVID-19 periods, no statistically significant difference was observed among HA-CDI rates per 10,000 bed-days (2.1 cases vs 1.76 cases; <em>P</em>=0.34), consumption of defined daily doses per 100 bed-days of antimicrobials – all antimicrobials (83.36 vs 89.5; <em>P</em>=0.091), fluoroquinolones only (3.71 vs 4.46; <em>P</em>=0.067), third-generation cephalosporins only (4.17 vs 4.43; <em>P</em>=0.449), carbapenems only (3.28 vs 3.26; <em>P</em>=0.944) – or the number of <em>C. difficile</em> tests performed per 10,000 bed-days (321.81 tests vs 326.63 tests; <em>P</em>=0.696).</p></div><div><h3>Conclusions</h3><p>There was no difference in the incidence rates of HA-CDI between COVID-19 and non-COVID-19 periods at our institution.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49855819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk of COVID-19 in different groups of healthcare professionals between February 2020 and June 2021 in Finland: a register-based cohort study","authors":"Sohvi Kääriäinen ,&nbsp;Ulla Harjunmaa ,&nbsp;Tuula Hannila-Handelberg ,&nbsp;Jukka Ollgren ,&nbsp;Outi Lyytikäinen","doi":"10.1016/j.infpip.2023.100297","DOIUrl":"10.1016/j.infpip.2023.100297","url":null,"abstract":"<div><h3>Background</h3><p>During the Coronavirus Disease 2019 (COVID-19) pandemic, healthcare workers (HCWs) have been a risk group for COVID-19.</p></div><div><h3>Aim</h3><p>To assess the cumulative incidence in different groups of HCWs and the risk factors and outcomes of COVID-19 in HCWs between February 2020 and June 2021 in Finland.</p></div><div><h3>Methods</h3><p>We linked two national registers, National Infectious Diseases Register (NIDR) and Register of Social Welfare and Healthcare Professionals (Terhikki), using national identity codes. COVID-19 cases were identified from NIDR notifications made by laboratories and physicians, and their healthcare professions from Terhikki. We categorized healthcare professions into seven groups and calculated cumulative incidences using Kaplan-Meier estimate during three periods (1/2/2020−30/6/2020, 1/7/2020−31/12/2020, 1/1/2021−30/6/2021). We identified risk factors in a multivariable model using Cox's regression.</p></div><div><h3>Findings</h3><p>We identified 8,009 COVID-19-cases among HCWs, with cumulative incidence of 1.79%; 83% were female, median age was 40.9 years (interquartile range, 31.2−51.6). Most COVID-19-cases occurred in nursing assistants (53%) and nurses (17%), with the highest cumulative incidences 2.07% (95%CI, 2.01−2.13%) and 1.82% (95%CI, 1.73−1.91%), respectively. Risk factors were male sex (hazard ratio (HR) 1.2; 95%CI, 1.1−1.3), foreign native language (HR 2.5; 95%CI, 2.2−2.9) and foreign country of birth (HR 1.2; 95%CI, 1.1−1.4). Physician notification data was available for 6,113/8,009 cases (76.3%); 244/6,113 (4.0%) were hospitalized and 37/6,113 (0.6%) in intensive care.</p></div><div><h3>Conclusion</h3><p>Nurses and nursing assistant, especially men and professionals with foreign background, were at higher risk of COVID-19. This should be specifically addressed during training and implementing infection control measures to protect themselves and patients.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c6/8a/main.PMC10406617.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-drug resistant Pseudomonas aeruginosa: a 2019–2020 single center retrospective case control study","authors":"Ann Fan Yang ,&nbsp;Vivian Huang ,&nbsp;Jevon Samaroo-Campbell ,&nbsp;Michael Augenbraun","doi":"10.1016/j.infpip.2023.100296","DOIUrl":"https://doi.org/10.1016/j.infpip.2023.100296","url":null,"abstract":"<div><p>Multi-drug resistance in the post COVID-19 world is a growing concern. The objective of this study was to describe temporal trends and explore independent risk factors for the isolation of multi-drug resistant (MDR) <em>P. aeruginosa.</em></p></div><div><h3>Methods</h3><p>This was a retrospective case-control study of patients with <em>P. aeruginosa</em> isolates recovered from January 2019 to December 2020. MDR <em>P. aeruginosa</em> was defined as non-susceptibility to at least one agent in three or more anti-pseudomonal antimicrobial categories.</p></div><div><h3>Results</h3><p>In total, 258 unique isolates were identified. Prolonged hospitalization (<em>P</em>&lt;0.001), prior antibiotic use (<em>P</em>&lt;0.001), and respiratory sources (<em>P</em>&lt;0.001) were strongly associated with the presence of MDR <em>P. aeruginosa</em>. From 2019 to 2020, there was a decrease in the total number of <em>P. aeruginosa</em> isolates but a significant increase in the proportion of MDR <em>P. aeruginosa</em> isolates (<em>P</em>=0.015).</p></div><div><h3>Conclusions</h3><p>Over a period that coincided with the COVID-19 pandemic, there was an increased proportion of MDR <em>P. aeruginosa</em> isolates from hospitalized patients. Improved identification of patients at risk for MDR <em>P. aeruginosa</em> could facilitate appropriate empiric antibiotic decisions like dual anti-pseudomonal therapy. The features of the COVID-19 outbreak that had a severe impact on patient care and that may have affected drug resistance in other respiratory pathogens should be explored.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49855818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudo-outbreak of haemodiafiltration dialysis fluid contamination: results of a detailed epidemiologic investigation","authors":"Renée Lévesque ,&nbsp;Patrice Savard ,&nbsp;Bernard Canaud","doi":"10.1016/j.infpip.2023.100292","DOIUrl":"10.1016/j.infpip.2023.100292","url":null,"abstract":"<div><h3>Background</h3><p>Compliance with dialysis fluid ultrapurity standards is a paramount for online modalities. More than 200 dialysis fluid samples have been analyzed monthly for years in our two dialysis units, with compliant microbiological results until mid-2020.</p></div><div><h3>Aim</h3><p>In mid-2020, an unusual occurrence (30%) of contaminated dialysis fluids in dialysis units led us to investigate to determine the source.</p></div><div><h3>Methods</h3><p>Microbiological methods for aquaphilic bacteria culturing and endotoxin detection in dialysis fluids were routinely performed on a monthly basis for all dialysis machines. As the contamination appeared randomly and almost simultaneously in our two units without any routine change or febrile syndrome, we searched for a common cause. Supplier's sampling kits as well as microbiological laboratory procedures were scrupulously investigated.</p></div><div><h3>Findings</h3><p>21 out of 30 sampling bags filled with sterile water brought back numerous fungi and bacteria. Laboratory's investigation, through the negative control tests performed routinely, exonerated the lab. All batches of bags analyzed later showed variable levels of contamination according to their transport/storage mode or date of manufacturing. Analyses performed by the supplier – methods complying with the medical device's standards but different from those recommended for dialysis fluids purity – remained negative.</p></div><div><h3>Conclusion</h3><p>Our investigation revealed that the contamination of our sampling kits came presumably from the manufacturer's supplying chain. Such false-positive results findings, created serious safety issues and disturbed clinical activities since positive machines were quarantined. Furthermore, it raised a serious concern about manufacturing, microbiological checking and shipping methods for the medical device industry that deserve further attention.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/eb/main.PMC10485658.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10224591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of SARS-CoV-2 RNA in exhaled breath and its potential for prevention measures","authors":"Madiha Malik,&nbsp;Thomas Kunze","doi":"10.1016/j.infpip.2023.100299","DOIUrl":"10.1016/j.infpip.2023.100299","url":null,"abstract":"<div><h3>Background</h3><p>To propose infection prevention measures it is essential to understand the dynamics of SARS-CoV-2 shedding, particularly in asymptomatic patients. This report compares the viral load progression in exhaled breath (EB) with the symptom severity. We aim to evaluate the adequacy of symptom assessment regarding the infectivity level of individuals.</p></div><div><h3>Methods</h3><p>We observed infected patients since their first positive test during hospitalization. EB samples were collected on days 1, 3, 5, 7, 10, 12 and 14 of hospitalization using a filter-based device. After extraction, viral loads were quantified with qRT-PCR. The infection trajectory was documented after symptom onset.</p></div><div><h3>Case Presentation and Discussion</h3><p>A 34-year old patient showed mild symptoms, e.g. fever, cough, headache, muscle pain and loss of taste and smell across trajectory of infection (Case 1). The viral loads emitted via exhaling were nearly constant and ranged from 8.6 x 10³ and 4.1 x 10⁴ RNA copies per hour. After the infection, the patient developed a pneumonia. The second case of a 65-year old patient depicted an asymptomatic infection trajectory for 14 days after the first diagnosis (Case 2). Nevertheless, the patient exhaled up to 2 x 10⁵ SARS-CoV-2 virus copies hourly, approximately 10 fold higher than measured for Case 1.</p></div><div><h3>Conclusion</h3><p>Symptomatic and asymptomatic COVID-19 patients exhale distinctive amounts of SARS-CoV-2 not necessarily correlating with symptom severity. Particularly, asymptomatic patients might show higher EB viral shedding. Therefore, EB testing should be included in infection prevention measures as it has high potential to reveal the most infectious individuals regardless of their symptoms during infection.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/26/7c/main.PMC10374965.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10267843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}