ISCC Indonesian Journal of Cancer Chemoprevention最新文献

{"title":"Treatment of Neuroendocrine Tumors (NETs) Using Somatostatin Analogs: Current View, Clinical Achievements and Future Perspectives","authors":"H. Wongso","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS2PP101-113","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS2PP101-113","url":null,"abstract":"Neuroendocrine tumors (NETs), previously known as carcinoid tumors, are a heterogeneous group of neoplasms which originate from cells of the endocrine or nervous system with an overall incidence of 1–5 cases per 100,000 individuals per year. Recent studies showed that their incidence has dramatically increased worldwide over the last few years. The majority of NETs overexpress the somatostatin receptors (SSTRs) in tumor cells which are further being used as the important targets for therapy purposes using somatostatin analogs (SSAs) such as octreotide and lanreotide. Like native somatostatin, SSAs bind to SSTRs and induce a range of cellular effects, including antitumor activity. Antitumor activity of SSAs and their high binding properties for the SSTRs have become valuable tools for developing advanced treatment of NETs. Consequently, SSAs have been used widely in routine clinical practice, especially for treatment of various types of NETs. Despite treatment using SSAs have made a positive contribution, recent development suggests that the used of SSAs in combination with chemotherapy or peptide receptor radionuclide therapy (PRRT) can improve clinical outcome in patients with NETs. This review provides an overview of recent trend in the treatment of NETs using SSAs, their clinical achievements in the last few years and their potential applications in the future.Keywords: neuroendocrine tumors, treatment, somatostatin analogs, chemotherapy, PRRTNeuroendocrine tumors (NETs), previously known as carcinoid tumors, are a heterogeneous group of neoplasms which originate from cells of the endocrine or nervous system with an overall incidence of 1–5 cases per 100,000 individuals per year. Recent studies showed that their incidence has dramatically increased worldwide over the last few years. The majority of NETs overexpress the somatostatin receptors (SSTRs) in tumor cells which are further being used as the important targets for therapy purposes using somatostatin analogs (SSAs) such as octreotide and lanreotide. Like native somatostatin, SSAs bind to SSTRs and induce a range of cellular effects, including antitumor activity. Antitumor activity of SSAs and their high binding properties for the SSTRs have become valuable tools for developing advanced treatment of NETs. Consequently, SSAs have been used widely in routine clinical practice, especially for treatment of various types of NETs. Despite treatment using SSAs have made a positive contribution, recent development suggests that the used of SSAs in combination with chemotherapy or peptide receptor radionuclide therapy (PRRT) can improve clinical outcome in patients with NETs. This review provides an overview of recent trend in the treatment of NETs using SSAs, their clinical achievements in the last few years and their potential applications in the future.Keywords: neuroendocrine tumors, treatment, somatostatin analogs, chemotherapy, PRRT","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85406625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of Murattal Al-Quran Audio Enhances Cisplatin Activity on Growth Inhibition and Cell Cycle Modulation on Hela Cells","authors":"R. Mutiah, Muhammad Ragib Mustofa, Yen yen Ari Indrawijaya, A. Hakim, R. Annisa, Nurlaili Susanti, Ach. Nashichuddin, Muhammad Zainuddin","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS2PP71-79","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS2PP71-79","url":null,"abstract":"Cancer is a disease characterized by abnormal cell mechanisms. The development of alternative cancer treatments is still needed. One of them is the music therapy. The music therapy uses sound vibrations to improve healing. Al-Quran, the Holy book of muslims, recites with measured recitation produces beautiful tones. Reading and recitating of Al-Quran is an important form of worship for which a Muslim can expect reward and benefit in the Hereafter. Al-Fatihah is one of surahs in Al-Qur'an that is often read by Muslims and used as a prayer for healing. The purpose of this study was to determine the effect of cytotoxic activity and cell cycle modulation on Hela cells with exposure of murattal Al-Quran and cisplatin combination. Audio exposure murattal Al-Fatihah and its combination with cisplatin to HeLa cells were tested using the MTT method assay. Induction of apoptosis and modulation of cell cycle evaluated by flow cytometry method. Treatment used 30 minutes Audio Murattal (AM), Cisplatin 10 µg/mL (Cis), and the combination of AM + Cis caused a decrease in the viability of HeLa cells respectively 80.14%, 69.86%, and 64.32%. The results of flow cytometry explained that in treatment of AM there was inhibition in the G2-M phase and induction of apoptosis in the M5 phase. Whereas in treatment AM + Cis inhibition occurs in the S, G2-M phase, and induction of apoptosis in the M5 phase. Audio Murattal Al-Quran presents cytotoxic effects on HeLa cells and to provide a synergistic impact on cisplatin so that disclosure therapy murattal can be recommended for supporting therapy in the treatment of cancer (supportive therapy).Keywords: Al-Quran, Audio murottal, HeLa Cells, Cell Viability, Flow cytometry","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81010747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of High Temperature Roasted Coffea liberica on Physiological and Histological Structure of Mice’s Kidney","authors":"Ivolanesky Putri Widodo, S. Syamsurizal, Diah Tri Utami","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS1PP30-36","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS1PP30-36","url":null,"abstract":"Numerous varieties of coffee, such as robusta, arabica, gayo and etcetera, was made in order to fulfill the needs of its lovers. In Tungkal, Jambi, Liberica coffee was recently discovered and the chemical reactions during roasting process produces several components, which are caffeine, chlorogenic acid, monosaccharides and others, to create a unique flavor as the identity. Unique flavor of Liberica coffee is obtained from the high temperature roasting process and the components in coffee. This research aims to determine the effect of Liberica coffee (Coffea liberica), concentration (0, 8.000, 10.000 and 12.000 ppm/day) on the physiological and histological structure of the mice’s kidney (Mus musculus L.) for 28 days. The results was showed that kidney histological observation of fatty degeneration, tubular swelling, and pyknotic nucleus associated with percentages of renal tubular damage in the control group, P1, P2 and P3 were 0.71±0.96, 4.91±0.17, 5.82±0.21 and 9.01±0.13 indicating low damage (<50%) and liberica coffee does not affect physiological function, such as creatinine level.Keywords : Liberica coffee, kidney, histological","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"256 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73039741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"in vitro Antidiabetic, Antioxidant and Cytotoxic Activities of Syzygium cumini Fractions from Leaves Ethanol Extract","authors":"N. Artanti, Faiza Maryani, R. Dewi, S. Handayani, I. D. Dewijanti, Lia Meilawati, Euis Filaila, L. Udin","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS1PP24-29","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS1PP24-29","url":null,"abstract":"Ethanol extract of Syzygium cumini (jamblang) leaves reveal its significant α-glucosidase inhibitory activity which perform the potential activity of this plant extract as antidiabetic agent. However, other bioactivities of this plant extract have been reported. The aim of current study was to evaluate the comparison of in vitro antidiabetic, antioxidant and cytotoxic activity of S. cumini fractions. The S. cumini leaves ethanol extract was fractionated by using column chromatography with n-hexane, ethyl acetate and methanol as eluents. Seven fractions were obtained from column chromatography (F1-7). The results showed that the best antidiabetic activity was found in F5 (93% α-glucosidase inhibitory activity), the best antioxidant activity was found in F4 (83% inhibition of DPPH free radical) and the best cytotoxic activity was found in F2 (69% growth inhibition of T47D breast cancer cell line). Therefore besides in vitro antidiabetic activity, fractions of S. cumini leaves ethanol extract also showed antioxidant and cytotoxic activities. Since the results showed that the most active fractions for antidiabetic, antioxidant and cytotoxicity were differed, it could be possible to isolate the different potential active compounds for each activity.Keywords : Syzygium cumini, α-glucosidase inhibitor, DPPH, cytotoxicity","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89711882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines","authors":"P. Hasibuan, P. Sitorus, D. Satria, Rizka Damela Sibuea","doi":"10.14499/indonesianjcanchemoprev10iss1pp37-45","DOIUrl":"https://doi.org/10.14499/indonesianjcanchemoprev10iss1pp37-45","url":null,"abstract":"Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 µg/mL(I), 127 µg/mL(II), 137 µg/mL(III), 129 µg/mL(IV), and 124 µg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 µg/mL, 46 µg/mL, 93 µg/mL, 71 µg/mL and 476 µg/mL; for T47D cell were 1621 µg/mL, 111 µg/mL, 128 µg/mL, 150 µg/mL and 209 µg/mL; and for MCF7 were 259 µg/mL, 343 µg/mL, 575 µg/mL, 408 µg/mL and 250 µg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90119990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different Culture Conditions","authors":"A. Santoso, Larasati Larasati, A. Kusumawati, P. H. Wisnuwardhani, Ratih Asma Ningrum, E. P. Septisetyani","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS1PP7-15","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS1PP7-15","url":null,"abstract":"Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable.Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"6 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72485372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Brief Outlook on Pharmacogenetics (PGx): Focus in MicroRNAs (miRNAs) and Cancer Stem Cells (CSCs)","authors":"F. Sandra","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS1PP46-50","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS1PP46-50","url":null,"abstract":"It has been known that there are differences in response to medications both in terms of clinical activity and side effects. Among all influencing factors, genetic variation has been considered to play a crucial part. By genetic investigation, the differences in drug metabolism, transport and target could be disclosed. Termed as “pharmacogenetics (PGx)”, that focuses on the variants within one or more candidate genes. Genetic tests have been started for screening polymorphisms prior to drug prescription, moreover many biomarkers were developed in oncology. Recent PGx investigations have been conducted to identify mRNAs, microRNAs (miRNAs) and other downstream signals that are affected by variation in genes that might cause drug response variability. Another intriguing study related to PGx in cancer stem cells (CSCs) has recently aroused. CSC shows more resistant behavior to drug. CSCs are subpopulation of cells, which share some same markers with stem cells. CSC can induce specific signal transduction pathways. Variation in genes affect CSCs activity are generally neglected in the past PGx studies. This could be one of the explanation why past PGx studies in cancer cell do not achieve optimal clinical outcome. Keywords : pharmacogenetics, pharmacogenomics, microRNAs, cancer stem cells","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81594133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression","authors":"Fitriasih Fitriasih, S. Komariyah, F. Sandra, Nenni Pratiwi, D. Hidayati","doi":"10.14499/indonesianjcanchemoprev10iss1pp1-6","DOIUrl":"https://doi.org/10.14499/indonesianjcanchemoprev10iss1pp1-6","url":null,"abstract":"Extract of Mangifera indica L. has been investigated to have cytotoxic activity against MCF-7 cells.  Current research was conducted to determine the cytotoxic activity of methanol extract of M. indica leaves (MEML) on MCF-7 breast cancer cells and to investigate on the MEML's effect on Bcl-2 and Bax expressions. M. indica leaves were extracted using soxhletation with methanol. The cytotoxic test was perfomed using MTT assay with various MEML concentrations: 15.625, 31.25, 62.5, 125, 250 and 1000 μg/mL. Meanwhile, Bax and Bcl-2 expressions of MCF-7 breast cancer cells were detected with immunocytochemical staining after treatment of MEML with concentrations of ¼ IC50, ½ IC50 and IC50. The results showed that IC50 of cytotoxic activity was 308.12 µg/mL. MEML could influence Bax and Bcl-2 protein expressions. After treatment of MEML, Bcl-2 expression was decreased while Bax expression was increased.Keywords : Mangifera indica L., Cytotoxic, Bax, Bcl-2, MCF-7","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72531355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caesalpinia sappan L. Ethanolic Extract Decrease Intracellular ROS Level and Senescence of 4T1 Breast Cancer Cells","authors":"Naufa Hanif, A. Hermawan, E. Meiyanto","doi":"10.14499/INDONESIANJCANCHEMOPREV10ISS1PP16-23","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV10ISS1PP16-23","url":null,"abstract":"Highly and uncontrolled cell proliferation on cancer cells may boost ROS level intracellular accumulation up significantly. Sappan heartwood (Caesalpinia sappan L.) have been known to have cytotoxicity effect toward several cancer cells. This research was conducted to develop Caesalpinia sappan L. heartwood ethanolic extract (CSE) as chemopreventive agent which can be used as cancer therapy, looked from antioxidant and anti-senescence activity toward 4T1 breast cancer cell. CSE was obtained through maceration using ethanol 70% as solvent. Cytotoxicity activity of CSE was done by using MTT assay with IC50 as parameter. This IC50 was used as basic to the next assay, ROS assay by using DCFDA staining flowcytometry to look ROS level intracellular of CSE toward 4T1 cell and senescence assay by using senescence associated β-galactosidase (SA β-gal) assay with % cell senescence as their parameter. CSE toxic to 4T1 cell that proved from IC50 value of 25 µg/mL. Single treatment of CSE on concentration 12,5; 25; and 37.5 µg/mL able to suppress ROS level intracellular. If compared with untreated cell, single treatment of CSE on concentration 6 and 12 µg/mL showed % cell senescence not significantly different. Based on this result, CSE is cytotoxic, has antioxidant and antisenescence activity, so it has great potential to developed as chemoprevention agent on cancer therapy.Keywords : Caesalpinia sappan L., 4T1 breast cancer line, Reactive Oxygen Species (ROS), anti-senescence","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"139 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89025153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic Activity of Ethyl Acetate Fraction of Aglaia elliptica Blume Leaves Extract on HepG2 Hepatocarcinoma Cells","authors":"Churiyah Churiyah, Ekclesia Yuliarni, A. E. Wibowo, Firdayani Firdayani","doi":"10.14499/indonesianjcanchemoprev9iss3pp143-151","DOIUrl":"https://doi.org/10.14499/indonesianjcanchemoprev9iss3pp143-151","url":null,"abstract":"Aglaia elliptica Blume belongs to Meliaceae family which contain an active compound of rocaglamid as anticancer. The research was conducted to evaluate the biological activities of ethyl acetate fraction of Aglaia elliptica Blume leaves. The dry powder of Aglaia elliptica Blume leaves was extracted with methanol using maceration method, then was fractionated using n-hexane and ethyl acetate, guided by brine shrimp lethality test (BSLT). The most toxic fraction then processed by column chromatography and resulted in eight subfractions. Three of them showed the most toxic effect on BSLT namely FEA 3.3, FEA 3.4 and FEA 3.5 with LC50 of 40.81, 18.56 and 13.40 ppm respectively. The cytotoxic activities of those three active subfractions were assayed on HepG2 hepatocarcinoma cells using enzymatic reaction of 3-(4,5-dimethylthiazoyl-2-yl) 2,5 diphenyltetrazolium bromide (MTT), and the results showed that the IC50 were 35.10, 14.36 and 14.09 ppm respectively. The most active sub fraction (FEA 3.5) was then performed further analyzed using preparative high performance liquid chromatography (HPLC). The HPLC results indicated that there were three active compounds, which were suspected as derivatives of rocaglamid. The molecular docking simulation indicated that rocaglamide formed complex with Toll-like Receptor 4 in HepG2 hepatocarcinoma cells and affected the inhibition of proliferation of its cell. Keywords : Aglaia elliptica Blume leaves extract, fractionation, BSLT, MTT assay,hepatocarsinoma cell lines","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85745374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}