ISCC Indonesian Journal of Cancer Chemoprevention最新文献_第7页

Pentagamaboronon-0 Fructose Inhibited Migration and Overexpression of Matrix Metalloproteinases 9 on MCF-7/HER2 Breast Cancer Cells 五加溴腺苷-0果糖抑制MCF-7/HER2乳腺癌细胞的迁移和基质金属蛋白酶9的过表达

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-10-30 DOI: 10.14499/indonesianjcanchemoprev9iss3pp134-142

I. Hairunisa, Rohmad Yudi Utomo, Yogi Ertanto, R. Jenie, E. Meiyanto

{"title":"Pentagamaboronon-0 Fructose Inhibited Migration and Overexpression of Matrix Metalloproteinases 9 on MCF-7/HER2 Breast Cancer Cells","authors":"I. Hairunisa, Rohmad Yudi Utomo, Yogi Ertanto, R. Jenie, E. Meiyanto","doi":"10.14499/indonesianjcanchemoprev9iss3pp134-142","DOIUrl":"https://doi.org/10.14499/indonesianjcanchemoprev9iss3pp134-142","url":null,"abstract":"The incidence of Breast Cancer Metastasis (MBC) can be categorized in stage IV as well as being the leading cause of death in cases of breast cancer. MBC prognosis is known to be weak, frequent recurrent, and MBC patients have only a survival rate of about 5 years. One of the proteins that causes breast cancer metastasis is Human Epidermal Growth Factor 2 (HER2). Pentagamaboron-0 (PGB-0), a newly curcumin analogue performed cytotoxic effect on HER2-positive breast cancer cells but it is practically water-insoluble. The aims of this study are to determine anti-metastatic activity of a more soluble form of PGB-0 namely PGB-0 fructose complex (PGB-0-F) toward HER2 positive cancer (MCF-7/HER2) cells. PGB-0-F was obtained from Cancer Chemoprevention Research Centre Faculty of Pharmacy Universitas Gadjah Mada. Based on scratch wound healing assay result, PGB-0-F inhibited cell migration especially in combination with doxorubicin at the concentration 15 μM. Under gelatin zymography assay, PGB-0-F in combination with doxorubicin decreased Matrix Metalloproteinases 9 (MMP-9) expression compare to the doxorubicin. Hence, PGB-0-F has a potency to be developed as anti-metastatic agent on HER2 overexpression breast cancer.Keywords : HER2, MCF-7/HER2, PGB-0-F, Metastasis","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83038891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Curcumin Analog Pentagamaboronon-0-Sorbitol Inhibits Cell Migration Activity of Triple Negative Breast Cancer Cell Line 姜黄素类似物五加甘露酮-0-山梨醇抑制三阴性乳腺癌细胞株细胞迁移活性

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-10-30 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS3PP126-133

Ratna D. Ramadani, Rohmad Yudi Utomo, A. Hermawan, E. Meiyanto

{"title":"Curcumin Analog Pentagamaboronon-0-Sorbitol Inhibits Cell Migration Activity of Triple Negative Breast Cancer Cell Line","authors":"Ratna D. Ramadani, Rohmad Yudi Utomo, A. Hermawan, E. Meiyanto","doi":"10.14499/INDONESIANJCANCHEMOPREV9ISS3PP126-133","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV9ISS3PP126-133","url":null,"abstract":"Mortality in cancer is primarily due to failure of metastasis prevention. One strategy to target the cancerous cell is Boron Neutron Captured Therapy which showed high affinity toward cancer cells and reported to have anti-proliferative as well as antimetastatic activities. Cancer Chemoprevention Research Center Faculty of Pharmacy Universitas Gadjah Mada, has developed boron-containing substance namely pentagamaboronon-0 (PGB-0) which is known to exhibit anticancer activity towards breast cancer cell. The purposes of this research are focused to explore the anti-migratory activities of PGB-0-So against triple negative breast cancer cell. The MTT cytotoxicity assay of PGB-0-So against 4T1 breast cancer cell line were found to exert potential effect in dose-dependent manner with IC50 values of 39 μM. The study of cell migration inhibition using in vitro wound healing assays and gelatin zymography on highly metastasis breast cancer cell line 4T1, following the treatment of sub IC50 doses of PGB-0-So complex slightly inhibited cell migration through the inhibition of matrix metalloproteinase-9 expression. These findings suggest that PGB-0-So is potential as an anticancer agent.Keywords : curcumin analogue, PGB-0-So, 4T1 Cells, migration, MMP-9 ","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77953776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Effect of Liberica Coffee on Liver Physiology and Histological Appearance of White Mice 利比里亚咖啡对小白鼠肝脏生理及组织学外观的影响

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-10-30 DOI: 10.14499/indonesianjcanchemoprev9iss3pp152-159

Cindra Ayuning Kirana, S. Syamsurizal, Diah Tri Utami

{"title":"The Effect of Liberica Coffee on Liver Physiology and Histological Appearance of White Mice","authors":"Cindra Ayuning Kirana, S. Syamsurizal, Diah Tri Utami","doi":"10.14499/indonesianjcanchemoprev9iss3pp152-159","DOIUrl":"https://doi.org/10.14499/indonesianjcanchemoprev9iss3pp152-159","url":null,"abstract":"Indonesia is one of the world's top coffee producing and exporting countries. Liberica coffee was one of the most widely cultivated types of coffee in Indonesia, especially in Tanjung Jabung Barat Jambi Province. Liberica coffee known as the coffee with highest levels of caffeine if compared with Arabica and Robusta coffee but the research about Liberica coffee was still very little if compared to the other two types of coffee. In the previous research, caffeine shown to be increasing alanine aminotransferase enzyme. Otherwise, coffee also contain chlorogenic acid which is a potent antioxidant and play a role in the protection of liver. The aim of this study are to investigate the effect of dark roasted Liberica coffee consumption on liver histological appearance, macroscopic anatomy and physiological function (serum glutamic-pyruvic transaminase (SGPT) level). This was an experimental study with posttest only control group design, using 45 male white mice and divided into 4 groups: control (with no coffee treatment), P1, P2 and P3 which were given coffee treatment consecutively 8000 ppm; 10.000 ppm and 12.000 ppm for 28 days. The result showed that liver histological damage level associated with the extent coffee intake. Although, the damage shown is still in a low level and has no impact in increasing SGPT level.Keywords : Liberica coffee, Liver, Histological appearance, SGPT level","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81041765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trigona sp. Propolis Ethanolic Extract Decreased Chloramphenicol-induced Serum Glutamic Oxaloacetic Transaminase and Alkaline Phosphatase Levels of Rats (Rattus novergicus) 蜂胶乙醇提取物降低氯霉素诱导大鼠血清谷草转氨酶和碱性磷酸酶水平

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-10-30 DOI: 10.14499/indonesianjcanchemoprev9iss3pp110-117

C. Mahdi, Anna Zukiaturrahmah, D. A. Pratama, Putranty Widha Nugraheni

{"title":"Trigona sp. Propolis Ethanolic Extract Decreased Chloramphenicol-induced Serum Glutamic Oxaloacetic Transaminase and Alkaline Phosphatase Levels of Rats (Rattus novergicus)","authors":"C. Mahdi, Anna Zukiaturrahmah, D. A. Pratama, Putranty Widha Nugraheni","doi":"10.14499/indonesianjcanchemoprev9iss3pp110-117","DOIUrl":"https://doi.org/10.14499/indonesianjcanchemoprev9iss3pp110-117","url":null,"abstract":"Liver has an important role in detoxification of toxins such as xenobiotic which could interfere the function of liver. Chloramphenicol is an antibiotic widely used,despite of its toxicity potentials. The enhancement of free radicals in the body could suppress antioxidant activity. Propolis of Trigona sp. has been known to contain very high amount of antioxidants. The enhanced serum glutamic oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) levels in serum is used as marker of liver damage due to the increase of free radicals. The purpose of this study was to observe the effect of Trigona sp. propolis ethanolic extract on SGOT and ALP levels in rats (Rattus novergicus) pretreated by chloramphenicol to induce liver damage. Test animals used for this research were male rats aged 8-12 weeks divided into five treatment groups: negative controlgroup (normal), positive control group (induced by 400 mg/kgBW chloramphenicol), first therapy group, second therapy group, third therapy group induced by chloramphenicol with and propolis extract with the dose of 8 mg, 16 mg, and 24 mg, respectively. Chloramphenicol was injected subcutaneously for 14 days, whereas propolis extract were administered orally for 21 days. The level of SGOT and ALP was determined using spectophotometry. The results showed that propolis extract could reduce levels of SGOT and ALP. Dose of 24 mg/kg was the effective dose to decrease levels of SGOT and ALP significantly (p<0.01). Hence, it may be concluded that the ethanol extract of propolis could be used as herbal therapy in rats model of liver damage.Keywords : ALP, liver, chloramphenicol, propolis, SGOT","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90695706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Anti-metastatic Activity of Curcumin Analog Pentagamaboronon-0-Sorbitol Against HER2-overexpressed MCF-7 Breast Cancer Cells 姜黄素类似物山梨醇对her2过表达的MCF-7乳腺癌细胞的抗转移活性

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-10-30 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS3PP118-125

Lailatul Qodria, I. Hairunisa, Rohmad Yudi Utomo, A. Hermawan, E. Meiyanto

{"title":"Anti-metastatic Activity of Curcumin Analog Pentagamaboronon-0-Sorbitol Against HER2-overexpressed MCF-7 Breast Cancer Cells","authors":"Lailatul Qodria, I. Hairunisa, Rohmad Yudi Utomo, A. Hermawan, E. Meiyanto","doi":"10.14499/INDONESIANJCANCHEMOPREV9ISS3PP118-125","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV9ISS3PP118-125","url":null,"abstract":"Breast cancer with Human Epidermal Growth Factor Receptor (HER)2 overexpression increases tumor progession and lead to metastasis, which is primarily cause of mortality in breast cancer. Pentagamaboronon-0 Sorbitol (PGB-0-So) is an aquoeous formulation of curcumin analog, PGB-0, with sorbitol. This compound has been developed as an anti-cancer chemotherapeutic agent and a boron carrying pharmaceutical for boron neutron capture therapy (BNCT). The aims of this study are to investigate antimetastatic activities of PGB-0-So against HER2-overexpressed MCF-7 breast cancer (MCF-7/HER2) cells. The MTT cytotoxicity assay of PGB-0-So exhibited cytotoxic effect with an IC50 value of 35 μM. The testing of anti-migration activity using the scratch wound healing assay demonstrated that PGB-0-So inhibited the closure of the wound on MCF-7/HER2 cells compare to the control. Furthermore, PGB-0-So was able to suppress matrix metalloproteinase (MMP)-9 activities, based on the gelatin zymography assay. In conclusion, PGB-0-So has potency to be developed as an anti-cancer agent against metastatic breast cancer.Keywords : PGB-0-So, anti-metastatsis, cell migration, MMP-9, MCF-7/HER2","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81986302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A Review: The Emerging Nutraceutical Potential of Pumpkin Seeds 南瓜籽的潜在营养价值

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-07-03 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS2PP92-101

Beni Lestari, E. Meiyanto

{"title":"A Review: The Emerging Nutraceutical Potential of Pumpkin Seeds","authors":"Beni Lestari, E. Meiyanto","doi":"10.14499/INDONESIANJCANCHEMOPREV9ISS2PP92-101","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV9ISS2PP92-101","url":null,"abstract":"The pumpkin, belongs to the family of Cucurbitaceae, is a well-known edible plant that has been frequently used as functional food or herbal medicine. Pumpkins contain rich unsaturated fatty acids, phytoestrogens and vitamins E in their seeds that have potential pharmaceutical, nutraceutical, and cosmeceutical properties. Information regarding their nutritional components and therapeutic properties of pumpkin seeds has expanded dynamically in the recent years and this review focus on the three main components of pumpkin seeds that described before. Several types of unsaturated fatty acids are the dominant composition in pumpkin seeds which can play a role in the disease prevention and promote health. Pumpkin seeds also contain the important phytoestrogen compounds, i.e., secoisolariciresinol and lariciresinol that have estrogenic-like effect such as preventing hyperlipidemia and osteoporosis for menopausal women. Phytoestrogens in pumpkin seeds also could be related to a reduced hormone-dependent tumor. Pumpkin seeds are rich in vitamin E contents as an emerging free radical scavenger, antiaging and antioxidant such as a-tocopherol and g-tocopherol. Findings of these studies prove that patents field for the innovation product of pumpkin seeds holds promise for the future along with their immense nutraceutical properties.","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"163 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73300974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Fingerroot (Boesenbergia pandurata) : A Prospective Anticancer Therapy 指根:一种有前景的抗癌疗法

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-07-03 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS2PP102-109

Marsya Yonna Nurrachma, Hilyatul Fadliyah, E. Meiyanto

引用次数: 8

Anti-metastatis Profile of Boesenbergia Pandurata towards MCF-7/HER2 Cells 潘杜拉草对MCF-7/HER2细胞的抗转移特征

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-07-03 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS2PP68-77

Hilyatul Fadliyah, Nindya Budiana Putri, Ziana Walidah, Ika Putri Nurhayati, Muthi’ Ikawati, E. Meiyanto

{"title":"Anti-metastatis Profile of Boesenbergia Pandurata towards MCF-7/HER2 Cells","authors":"Hilyatul Fadliyah, Nindya Budiana Putri, Ziana Walidah, Ika Putri Nurhayati, Muthi’ Ikawati, E. Meiyanto","doi":"10.14499/INDONESIANJCANCHEMOPREV9ISS2PP68-77","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV9ISS2PP68-77","url":null,"abstract":" The development of cancer at an advanced stage is signed with metastasis that trigger the high of mortality caused breast cancer, possitive HER2 type mainly. Boesenbergia pandurata known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory of migration of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin (Dox) on MCF-7/HER2 cells. BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23 ± 3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that BPEE (5 μg/mL) and its combination with doxorubicin (10 nM) inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of MMP2 and MMP9 proteins, but single treatment of BPEE was more able to decrease the expression of both MMP. The combination with those concentrations inhibited the cells migration but not with the cells viability. Thus, BPEE is potential to be developed as antimetastatic agent. The mechanism itself needs to be explored further.","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76448997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Estrogenic Activity of Ethanolic Extract of Papaya Peels (Carica Papaya L.) on Uterine Weight and Mammae Gland Proliferation on Ovariectomy Rats 番木瓜皮乙醇提取物对卵巢切除大鼠子宫重量和乳腺增生的雌激素活性

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-07-03 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS2PP86-91

Dhania Novitasari, Devyanto Hadi Triutomo, F. Arifah, Anselma Ivanawati, Zahrotul Ulum, R. Murwanti

{"title":"Estrogenic Activity of Ethanolic Extract of Papaya Peels (Carica Papaya L.) on Uterine Weight and Mammae Gland Proliferation on Ovariectomy Rats","authors":"Dhania Novitasari, Devyanto Hadi Triutomo, F. Arifah, Anselma Ivanawati, Zahrotul Ulum, R. Murwanti","doi":"10.14499/INDONESIANJCANCHEMOPREV9ISS2PP86-91","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV9ISS2PP86-91","url":null,"abstract":"Papaya bark is one of Indonesia's natural wealth that contains flavonoid compounds such as myricetin and kaempferol that included in the phytoestrogen compounds. The aim of this study is to examine the estrogenic effects of ethanolic extract of papaya peels (EEPP), on the development of mammae gland and the increasing of uterine weight. The in vivo test was performed with ovariectomy in Sprague Dawley female rats that caused the rats to be in an estrogen deficiency state. After 30 days of treatment, animals are sacrificed to take the uterus and mammae glands. Measurement of uterine weight and mammae gland are observed by hematoxylin-eosin staining method to know the lobulus development and AgNOR staining to determine the proliferation level of mammae gland epithelial cells. The test results showed that EEPP concentration of 500 and 1000 mg/kgBW were able to increase uterine weight and proliferation of mammae gland. From the results of this study, papaya bark has the potential to be one of the phytoestrogens compound to maintain female reproductive health and woman beauty.Keyword: ethanolic extract of papaya peels (EEPP), phytoestrogen, ovariectomized rats, uterine weight, mammae proliferation","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85464400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

RANKL and TNF-α-induced JNK/SAPK Osteoclastogenic Signaling Pathway was Inhibited by Caffeic Acid in RAW-D Cells 咖啡酸对rawd细胞中RANKL和TNF-α诱导的JNK/SAPK破骨信号通路的抑制作用

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2018-07-03 DOI: 10.14499/INDONESIANJCANCHEMOPREV9ISS2PP63-67

F. Sandra, Junita Briskila, Ketherin Ketherin

{"title":"RANKL and TNF-α-induced JNK/SAPK Osteoclastogenic Signaling Pathway was Inhibited by Caffeic Acid in RAW-D Cells","authors":"F. Sandra, Junita Briskila, Ketherin Ketherin","doi":"10.14499/INDONESIANJCANCHEMOPREV9ISS2PP63-67","DOIUrl":"https://doi.org/10.14499/INDONESIANJCANCHEMOPREV9ISS2PP63-67","url":null,"abstract":"Caffeic acid, a natural substance found majorly in fruits, grains, and herbs, is known to have therapeutic benefits. One of which is to inhibit bone resorption by targeting osteoclastogenesis through inhibition of the Cathepsin K, p38 Mitogen-activated Protein Kinase (MAPK), Nuclear Factor of Activated T-cells c1 (NFATc1) and Nuclear Factor kB (NFkB). Besides p38 MAPK, the c-Jun N-terminal kinase (JNK) / stress-activated protein kinases (SAPK), another member of MAPK family, has been reported to play important roles in osteoclastogenesis. Hence, current study was undertaken in order to investigate mechanism of Caffeic Acid towards JNK/SAPK pathway. Tartrate Resistant Acid Phosphatase (TRAP) staining was performed on caffeic acid-treated and RANKL-TNFα-induced RAW-D cells. Western blot analysis was performed to detect JNK/SAPK and phosphorylated-JNK/SAPK. Protein bands were quantified and statistically analyzed. Treatment of 10 μg/mL Caffeic Acid inhibited 20 ng/mL RANKL and 1 ng/mL TNFα-induced RAW-D differentiation into TRAP+ osteoclast-like polynuclear cells. Induction of 20 ng/mL of RANKL and 1 ng/mL of TNFα for 0.2 or 1 hour, significantly increase phosphorylation of JNK/SAPK as compared with control. Treatment of 10 µg/mL Caffeic Acid significantly inhibited the 20 ng/mL of RANKL and 1 ng/mL of TNFα-induced phosphorylation of JNK/SAPK. Taken together, Caffeic Acid could inhibit the RANKL and TNFα-induced osteoclastogenesis through JNK/SAPK.","PeriodicalId":32620,"journal":{"name":"ISCC Indonesian Journal of Cancer Chemoprevention","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91007248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6