Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression

ISCC Indonesian Journal of Cancer Chemoprevention Pub Date : 2019-02-01 DOI:10.14499/indonesianjcanchemoprev10iss1pp1-6

Fitriasih Fitriasih, S. Komariyah, F. Sandra, Nenni Pratiwi, D. Hidayati

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引用次数: 2

Abstract

Extract of Mangifera indica L. has been investigated to have cytotoxic activity against MCF-7 cells. Current research was conducted to determine the cytotoxic activity of methanol extract of M. indica leaves (MEML) on MCF-7 breast cancer cells and to investigate on the MEML's effect on Bcl-2 and Bax expressions. M. indica leaves were extracted using soxhletation with methanol. The cytotoxic test was perfomed using MTT assay with various MEML concentrations: 15.625, 31.25, 62.5, 125, 250 and 1000 μg/mL. Meanwhile, Bax and Bcl-2 expressions of MCF-7 breast cancer cells were detected with immunocytochemical staining after treatment of MEML with concentrations of ¼ IC50, ½ IC50 and IC50. The results showed that IC50 of cytotoxic activity was 308.12 µg/mL. MEML could influence Bax and Bcl-2 protein expressions. After treatment of MEML, Bcl-2 expression was decreased while Bax expression was increased.Keywords : Mangifera indica L., Cytotoxic, Bax, Bcl-2, MCF-7

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芒果叶提取物通过降低Bcl-2表达和诱导Bax表达诱导MCF-7细胞内在凋亡通路

研究了芒果提取物对MCF-7细胞的细胞毒活性。本研究旨在检测籼米叶甲醇提取物(MEML)对MCF-7乳腺癌细胞的细胞毒活性，并探讨MEML对Bcl-2和Bax表达的影响。采用甲醇蒸馏法提取籼稻叶片。采用MTT法对不同浓度的MEML(15.625、31.25、62.5、125、250、1000 μg/mL)进行细胞毒试验。同时免疫细胞化学染色检测MEML在¼IC50、½IC50和IC50浓度下作用MCF-7乳腺癌细胞后Bax和Bcl-2的表达。结果表明，其细胞毒活性IC50为308.12µg/mL。MEML可影响Bax和Bcl-2蛋白的表达。经MEML处理后，Bcl-2表达降低，Bax表达升高。关键词:芒果，细胞毒性，Bax, Bcl-2, MCF-7

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来源期刊

ISCC Indonesian Journal of Cancer Chemoprevention

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审稿时长

20 weeks