Ege Tip Dergisi最新文献

{"title":"Combinational effects of ponatinib and some Turkish endemic plant extracts on breast cancer cells","authors":"Çağla Kayabaşı, Ç. Avcı, Sunde Yilmaz Süslüer, Tuğçe Balcı Okcanoğlu, Besra ÖZMEN YELKEN, Cansu Caliskan Kurt, Bakiye Göker Bağca, C. Durmuskahya, H. Kayalar, A. Özbilgin, Cumhur Gündüz","doi":"10.19161/etd.1126901","DOIUrl":"https://doi.org/10.19161/etd.1126901","url":null,"abstract":"Aim: Breast cancer is the most common malignancy in women worldwide. Therefore, there is a need to define new strategies that can overcome the deficiencies of existing treatments. In our study, we aimed to define new herbal combination therapies that can be used to target breast cancer cells. For this purpose, we investigated the cytotoxic, apoptotic, anti-proliferative and cell cycle regulatory effects of Centaurea calolepis (CCI), Origanum sipyleum (OSM) and Phlomis lycia (PLI) plant extracts in combination with ponatinib on MCF-7 cells. \u0000Materials and Methods: The cytotoxic effects of OSM, CCI, PLI and ponatinib on MCF-7 cells were measured in real time by xCELLigence. The median-effect equation was used for the analysis of combinations of ponatinib with CCI (p-CCI), OSM (p-OSM), PLI (p-PLI). Apoptosis, proliferation and cell cycle regulation were evaluated by flow cytometry. \u0000Results: The IC50 doses of CCI, OSM and PLI extracts in MCF-7 cells were calculated as 59.5, 57, 44.2 μg/ml at 48 hours and 51.6, 54.21, 42.52 μg/ml at 72 hours, respectively. Combination analyses revealed that p-CCI was additive, p-OSM and p-PLI showed a moderate synergistic effect at 48th hours. It was determined that apoptosis induced by ponatinib was significantly increased with the combinations of CCI and PLI. CCI and PLI treatments exhibited moderate anti-proliferative effects on MCF-7 cells, while OSM extract suppressed proliferation most significantly. Consistent with the proliferation results, the highest G0/G1 arrest was observed with OSM treatment. It was revealed that combined p-CCI and p-PLI treatments significantly increased the anti-proliferative effect of ponatinib and caused a higher level of G0/G1 accumulation. \u0000Conclusion: Combinations of ponatinib and CCI, OSM, PLI plant extracts exhibited anti-cancer activity in breast cancer with induction of apoptosis, suppression of proliferation and cell cycle arrest. In light of the high anti-cancer effects identified, extracts of these Turkish endemic plants may represent a potential strategy in the treatment of breast cancer patients.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corneal biomechanics and cornea assessment in Fuchs heterochromic iridocyclitis patients","authors":"Semir Yarımada, Cumali Değirmenci, M. Palamar, Suzan Güven Yılmaz, H. Ateş","doi":"10.19161/etd.1127207","DOIUrl":"https://doi.org/10.19161/etd.1127207","url":null,"abstract":"Aim: To evaluate cornea and corneal biomechanical properties of patients with Fuchs heterochromic iridocylitis. \u0000Materials and Methods: Fourteen FHI positive eyes (Group 1) and the contralateral healthy eyes (Group 2) were included. All patients underwent a detailed ophthalmic examination was performed. Also, Ocular Response Analyzer was used to detect corneal biomechanical properties, and specular microscopic evaluation for corneal endothelial cells count was performed. \u0000Results: The mean best corrected visual acuity and intraocular pressure were statistically similar. (p values were 0.077 and 0.557, respectively). Corneal biomechanical properties including corneal hysteresis, corneal resistance factor, IOPcc and IOPg were not statistically significant. (p values were 0.521, 0.817, 0.980 and 0.980, respectively). \u0000Mean central corneal thickness in Group 1 and 2 were 555.57±42.95 (467-626), 556.5±37.04 (480-623) micrometer, respectively. The difference was not statistically significant (p=0.959). Mean corneal endothelial cell density of Group 1 and Group 2 were 2313±420.22 (1271-2717) and 2404.42±326.75 (1566-2834) cells/mm2, respectively. The difference was not statistically significant (p=0.626). \u0000Conclusion: Conflicting with the literature, no differences were detected in corneal biomechanical properties, central corneal thickness and corneal endothelial cell density in Fuchs Heterochromic Iridocyclitis positive eyes. However, more studies with increasing number of patients are still needed.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oldukça nadir bir koroner arter anomalisi: Non-koroner sinüsten çıkan sol ana koroner arter","authors":"Cihat Pinarci, Selen Bayraktaroğlu","doi":"10.19161/etd.1127945","DOIUrl":"https://doi.org/10.19161/etd.1127945","url":null,"abstract":"Although they are rare, left coronary artery anomalies can cause complications with high mortality, including arrhythmia and cardiac arrest. Left main coronary artery originates from the non-coronary sinus abnormality is rarely observed. This abnormality has been defined as a benign pathology in the past, although it is described in the literature to cause morbidity and mortality in some cases. We present very rare case of the left main coronary artery arising from non-coronary sinus detected on CCTA.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Çocuklarda ani sensörinöral işitme kaybı: etiyoloji, prognostik faktörler ve tedavi sonuçları","authors":"Abdulhalim Aysel, A. Dalgic, Togay Müderri̇s, Fatih Yılmaz, Görkem Atsal, Hayal Boyacioğlu, Özgür ÖZDEMİR ŞİMŞEK, Enver Altaş","doi":"10.19161/etd.1126855","DOIUrl":"https://doi.org/10.19161/etd.1126855","url":null,"abstract":"Aim: Sudden sensorineural hearing loss (SSNHL) may have a negative impact on the language and psychological development of children, especially if it is not diagnosed early and treated promptly. This study were aimed to determine and compare the etiological factors, treatment outcomes and prognostic factors in the pediatric patients who were followed up with the diagnosis of SSNHL. \u0000Materials and Methods: The files of 28 children were analyzed retrospectively. In pure tone audiometry, the average of pure tone thresholds of 500 Hz, 1000 Hz, 2000 Hz and 4000 Hz were accepted as pure tone averages (PTAV). Patients' recovery status was determined according to Siegel criteria. Audiometric curve types were evaluated as ascending, descending, and flat. \u0000Results: The ages of patients with SSNHL was mean ± SD 14.89 ± 3.24 (min-max: 7 and 18). The pre-treatment PTAV was mean ± SD 55.27 ± 12.39 dB HL (min-max: 38.5 and 85.25 dB HL) and the PTAV after treatment was mean ± SD 23.13 ± 18.22 dB HL (min-max: 5 and 72.5 dB HL). Audiometric curve types were detected as descending curve (n: 11, 39.2%), ascending curve (n: 5, 17.9%), flat curve (n: 12, 44.9%). Eighteen (64.3%) patients had complete recovery, 8 (28.6%) patients had partial recovery, and 2 (7.1%) patients had no recovery after the treatment. \u0000Conclusion: The response to treatment was found to be high. Descending audiometric curve type was found as a positive prognostic factor. Although the presence of tinnitus was a better prognostic factor than the presence of vertigo.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of tumoral glypican 3 mRNA level as a diagnostic and prognostic biomarker for hepatitis-b virus-associated hepatocellular carcinoma by an integrative transcriptomic meta-analysis and bioinformatics","authors":"S. Atay","doi":"10.19161/etd.1127225","DOIUrl":"https://doi.org/10.19161/etd.1127225","url":null,"abstract":"Aim: The aim of this study is to evaluate the potential of GPC3 mRNA level as a diagnostic and prognostic biomarker for HBV-associated HCC. Materials and Methods: GPC3 mRNA expression in HBV-associated HCC tumor tissues compared to matched adjacent tissues was evaluated by integrative transcriptomic meta-analysis. The results were validated in a different patient cohort and the possible associations between GPC3 mRNA level and the clinical variables were evaluated. \u0000Results: Transcriptomic data of HBV-associated HCC tissues (n=61) and matched adjacent tissues (n=61) from four datasets (GSE19665;GSE84402;GSE121248;GSE55092) were included in the meta-analysis. GPC3 mRNA level was found to be higher in tumors than adjacent tissues (fold change=12.88; p= 0;FDR=0). The result was validated in GSE14520, (HBV-associated HCC(n)=203; matched adjacent tissue(n)=203), (log-fold-change= 4.82; adj.p=1.43E-79). It was found that GPC3 mRNA level could distinguish HCC from adjacent tissues with high specificity and sensitivity (AUC=0.9108;95%CI=0.08792-0.9424;p","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67799579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective features of cases with cranial magnetic resonance imaging","authors":"Hilal Aydin, S. Yazıcı, Ezgi Özden, G. Demirpolat","doi":"10.19161/etd.1126762","DOIUrl":"https://doi.org/10.19161/etd.1126762","url":null,"abstract":"Aim: In this study, we aimed to reveal the diagnostic profiles of 0-18 years aged patients, for whom cranial magnetic resonance imaging is requested in outpatient settings, and to evaluate the cranial imaging results according to gender and age groups. \u0000Materials and Methods: The files of patients aged 0-18 years who were requested cranial magnetic resonance imaging for various indications, between August 2019 and March 2021, in Balikesir University, Faculty of Medicine pediatric neurology and pediatric health and diseases outpatient clinics were reviewed retrospectively. \u0000Age, gender, main complaint and neuroradiological imaging results were obtained from hospital records. Data were divided for three different age groups (0-6, 7-12, 13-18). \u0000Results: Cranial magnetic resonance imaging of 313 cases were analyzed. The mean age of the patients was 9.35±4.89 (4 months-17 years) years. There were 164 (52.4%) boys and 149 (47.6%) girls. There were 82 (26.2%) cases in the 0-6 years age group, 104 (33.2%) in the 7-12 years age group and 127 (40.6%) in the 13-18 years age group. The most common reasons for requesting cranial magnetic resonance imaging were as; seizure/epilepsy in 106 (33.9%) cases, headache in 65 (20.8%) cases, and neuromotor retardation in 28 (8.9%) cases. While the cranial imaging of 200 (63.9%) cases was normal, the results of 113 (36.1%) cases were evaluated as abnormal. The most common abnormal findings were intracranial mass (2.5%), nonspecific white matter lesion (5.1%), intracranial cyst (5.7%), sinusitis (9.2%) and hydrocephalus/hydrancephaly (2.6%). When age groups were compared in terms of showing normal or abnormal cranial imaging findings, no statistically significant difference was found (p=0.73), but a statistically significant difference was found between the genders in the same respect (p=0.007). \u0000Conclusion: Our study is important for including cranial MRI request indications and results in pediatric practice and it creating a diagnostic profile in these patients.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67798631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 Pandemisi tıp öğrencilerinin ve uzmanlık öğrencilerinin el hijyenine uyumunu değiştiriyor mu? Bir üniversite hastanesinden karşılaştırmalı çalışma","authors":"Özlem Coşkun, I. Budakoğlu, Yavuz Selim Kiyak","doi":"10.19161/etd.1126614","DOIUrl":"https://doi.org/10.19161/etd.1126614","url":null,"abstract":"Aim: We aimed to find out if there is statistically significant difference in self-reported hand hygiene compliance among Year-5 medical students, Year-6 medical students, and residents in Gazi University Faculty of Medicine between right before and after the first confirmed case of COVID-19 in Turkiye. \u0000Materials and Methods: Two cross-sectional surveys were carried out to reveal self-reported hand hygiene compliance among participants right before and after the first confirmed case of COVID-19 in Turkiye. Participants were Year-5 and Year-6 medical students and residents in Gazi University Faculty of Medicine. Convenience sampling was used. There were 833 participants in total. The survey form consists of 10 items that were constituted by examining World Health Organization guidelines on hand hygiene. The compliance scores before and after the first confirmed case were compared by performing T-Test. \u0000Results: Out of 10 items, there was significant difference in Year-5 medical students’, Year-6 medical students’ and residents’ self-reported hand hygiene compliance scores between before and after the first confirmed case of COVID-19 in 6 items, 10 items, and 8 items, respectively (p","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49072174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hastanede yatan yeni Coronavirus 2019 hastalarının gözyaşı örneklerinde Severe Acute Respiratory Syndrome Coronavirus-2 RNA’larının değerlendirilmesi","authors":"Nilüfer ZORLUTUNA KAYMAK, Gizem DOĞAN GÖKÇE, M. Tezcan, Serap DEMİR TEKOL","doi":"10.19161/etd.1125211","DOIUrl":"https://doi.org/10.19161/etd.1125211","url":null,"abstract":"Aim: We aimed to detect the presence of Severe Acute Respiratory Syndrome Coronavirus-2 RNA in the tears of patients with confirmed novel Coronavirus disease 2019. \u0000Materials and Methods: This prospective study was performed at Health Science University Kartal Dr. Lutfi Kırdar City Hospital between 13-21 May 2020. Nasopharyngeal and tear samples of 15 patients with novel Coronavirus disease 2019 was performed for real-time polymerase chain reaction assay. Ocular and systemic signs and symptoms, chest computed tomographic scans, and results of laboratory blood tests as well as the drugs used for the treatment were noted and analyzed. \u0000Results: Of the 15 patients nine (60%) were male and six (40%) were female. Mean age of the patients was 53.86±20.20 (21-89) years. Among them nine patients (60%) were positive for novel coronavirus disease 2019 on real-time polymerase chain reaction from nasopharyngeal specimens. None of the patients was positive for real-time polymerase chain reaction for Severe Acute Respiratory Syndrome Coronavirus-2 in tear specimens. Only two of 15 patients had bilateral conjunctivitis. These two patients had positive results for Severe Acute Respiratory Syndrome Coronavirus-2 on real-time polymerase chain reaction from nasopharyngeal swabs. \u0000Conclusion: In this study Severe Acute Respiratory Syndrome Coronavirus-2 RNA could not be detected in the tear samples of the patients with confirmed novel Coronavirus disease 2019 with or without ocular symptoms and signs.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47498055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperatif tam kan sayımı belirteçleri ve De-Ritis oranının testis tümörünün tanısındaki prediktif yeri","authors":"Hakan Bahadır Haberal, Kubilay Sarikaya, Fahri Erkan Sadioğlu, Muhammed Arif İbiş, Çağrı Şenocak, Ö. Bozkurt","doi":"10.19161/etd.1125252","DOIUrl":"https://doi.org/10.19161/etd.1125252","url":null,"abstract":"Aim: To determine the value of neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), lymphocyte-monocyte ratio (LMR), systemic immune-inflammation (SII) index and De-Ritis ratio, which are among preoperative whole blood and biochemical parameters, in the diagnosis of testicular tumor. \u0000Materials and Methods: The data of patients who underwent inguinal orchiectomy for testicular tumor in our clinic between October 2010 and December 2019 and patients who underwent varicocelectomy, as a control group, were retrospectively analyzed. Patients with missing data, under 18 years of age or with additional morbidity were excluded from the present study. Prediction values for NLR, PLR, LMR, SII index and De-Ritis ratio were determined and the value of these parameters in the diagnosis of testicular tumor was examined. \u0000Results: Thirty-four (14.1%) patients underwent inguinal orchiectomy while 207 (85.9%) patients underwent varicocelectomy in the present study. The median age of the patients during surgery was 27 (23-32) years. The number of patients with seminoma, mixt germ cell tumor, yolk sac tumor and embryonal carcinoma was 18 (52.9%), 12 (35.3%), 3 (8.8%) and 1 (2.9%), respectively. It was determined that 61.8% of the patients with testicular tumors were in the T2 stage and 35.3% had metastasis. The cut-off values for NLR, PLR, LMR and SII index were determined as 1.76, 133.43, 7.81 and 571.63, respectively. There was no statistically significant cut-off value for De-Ritis ratio (p = 0.183). The only significant factor for predicting testicular tumor was SII index in multivariate analysis (p","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67798550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yanık ünitesinde dört yıllık tecrübe ile literatür analizi","authors":"A. Şahin, Murat Sabri Yilmaz","doi":"10.19161/etd.1125591","DOIUrl":"https://doi.org/10.19161/etd.1125591","url":null,"abstract":"Aim: Burn is a particular form of trauma that can occur with physical and/or chemical factors, affect all systems of organism, and be seen in all ages and genders. In our study, a retrospective analysis was made on the data of children treated in our burn unit over 4 years. Materials and Methods: The data of 266 patients hospitalized in the Pediatric Surgery Clinic's burn unit between January 2017 and December 2020 were obtained retrospectively from the hospital information management system. Results: In four years, the number of patients who were evaluated was 266. Ninety-seven of these patients are girls, and 169 of them are boys. The average age of all patients is 2.86 years. (Average: 2.92 for girls, 2.81 for boys). Conclusion: Patient treatments are customized according to the depth and width of the burn. With an approach specific to each child, individualized treatment plans, which consider the quality of life of the child and parents, will increase the effectiveness of the treatment. Individualized treatment plans, with specific approaches towards each child in consideration of the quality of life of the child and/or their parents, would increase the effectiveness of the treatments.","PeriodicalId":32499,"journal":{"name":"Ege Tip Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67798946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}