通过综合转录组荟萃分析和生物信息学评估肿瘤glypican 3mrna水平作为乙型肝炎病毒相关肝细胞癌的诊断和预后生物标志物

Ege Tip Dergisi Pub Date : 2022-06-13 DOI:10.19161/etd.1127225

S. Atay

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引用次数: 0

摘要

目的:本研究的目的是评估GPC3 mRNA水平作为hbv相关HCC的诊断和预后生物标志物的潜力。材料和方法:通过整合转录组meta分析评估hbv相关HCC肿瘤组织与匹配邻近组织中GPC3 mRNA的表达。在不同的患者队列中验证了结果，并评估了GPC3 mRNA水平与临床变量之间可能存在的关联。结果:来自四个数据集(GSE19665;GSE84402;GSE121248;GSE55092)的hbv相关HCC组织(n=61)和匹配的邻近组织(n=61)的转录组学数据被纳入meta分析。肿瘤组织中GPC3 mRNA水平高于癌旁组织(倍数变化=12.88;p = 0;罗斯福= 0)。结果在GSE14520中得到验证，(hbv相关HCC(n)=203;匹配的邻近组织(n)=203)， (log-fold-change= 4.82;adj.p = 1.43 e - 79)。结果发现，GPC3 mRNA水平可区分HCC与邻近组织，具有较高的特异性和敏感性(AUC=0.9108;95%CI=0.08792-0.9424

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Evaluation of tumoral glypican 3 mRNA level as a diagnostic and prognostic biomarker for hepatitis-b virus-associated hepatocellular carcinoma by an integrative transcriptomic meta-analysis and bioinformatics

Aim: The aim of this study is to evaluate the potential of GPC3 mRNA level as a diagnostic and prognostic biomarker for HBV-associated HCC. Materials and Methods: GPC3 mRNA expression in HBV-associated HCC tumor tissues compared to matched adjacent tissues was evaluated by integrative transcriptomic meta-analysis. The results were validated in a different patient cohort and the possible associations between GPC3 mRNA level and the clinical variables were evaluated. Results: Transcriptomic data of HBV-associated HCC tissues (n=61) and matched adjacent tissues (n=61) from four datasets (GSE19665;GSE84402;GSE121248;GSE55092) were included in the meta-analysis. GPC3 mRNA level was found to be higher in tumors than adjacent tissues (fold change=12.88; p= 0;FDR=0). The result was validated in GSE14520, (HBV-associated HCC(n)=203; matched adjacent tissue(n)=203), (log-fold-change= 4.82; adj.p=1.43E-79). It was found that GPC3 mRNA level could distinguish HCC from adjacent tissues with high specificity and sensitivity (AUC=0.9108;95%CI=0.08792-0.9424;p

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Ege Tip Dergisi

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审稿时长

7 weeks