Buletin Penelitian Tanaman Rempah dan Obat最新文献

{"title":"Effectiveness of Ethanol Extract of Neem, Mahogany and Surian Seeds on Tenebrio molitor Linnaeus Larva (Tenebrionidae : Coleoptera)","authors":"W. Darwiati, U. W. Darmawan, C. Syukur","doi":"10.21082/bullittro.v31n1.2020.40-47","DOIUrl":"https://doi.org/10.21082/bullittro.v31n1.2020.40-47","url":null,"abstract":"<em>Neem, mahogany, and surian seeds contain secondary metabolites that can be used as biopesticides. This study aimed to determine the effectiveness of neem, mahogany, and surian seed extracts to the larvae of the </em>Tenebrio molitor<em> L. (Tenebrionidae: Coleoptera) </em><em>at laboratory scale. The stages of the research were the preparation of seed extracts, phytochemical analysis of the extracts, and efficacy of extracts against </em>T. molitor<em> larvae. Dried seeds of neem, sur</em><em>ian, and mahogany (water content < 10%) were ground (100 mesh) and macerated with 95% ethanol solvent for seven hours at 70 ^oC. The alkaloids, flavonoids, tannins, saponins, and steroids/the triterpenoid were then analyzed. The effectiveness of the extracts was  tested</em><em>against the 3^rd larva of </em>T. molinor<em>. The experiment</em><em> designed in Randomize block design, three replications, each with ten larvae. The first factor was seed extracts (neem, surian, and mahogany), while the second factor was five levels of extract concentration (</em><em>0 mg;l^-1, 9 mg.l^-1, 15 mg.l^-1, 30 mg.l^-1 and 45 mg.l^-1). Phytochemical analysis showed that mahogany seeds contained alkaloids and triterpenoids. Meanwhile, surian seeds contained tannin, alkaloid, steroid/triterpenoid, but neem seeds contained saponin and alkaloids. The mahogany, surian, and neem seed  extracts were effective in causing significant mortality to </em>T. molitor<em> larvae. Ethanol extract of neem seeds was more toxic to larvae (LC₅₀ = 0.72 mg.l^-1), compared to mahogany (LC₅₀ = 5.2 mg.l^-1) and sur</em><em>ian (LC₅₀ = 7.02 mg.l^-1). Mimba seed extract is more prospective to be developed as a botanical insecticide.</em>","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":"71 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41324331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas In Vitro Penurun Gula Darah dari Ekstrak Buah Parijoto (Medinilla speciosa B.)","authors":"R. Vifta, Nfn Wilantika, Yustisia Dian Advistasari","doi":"10.21082/BULLITTRO.V31N1.2020.31-39","DOIUrl":"https://doi.org/10.21082/BULLITTRO.V31N1.2020.31-39","url":null,"abstract":"Diabetes mellitus is one of the highest causes of death in the world, with symptoms of increased blood glucose levels (hyperglycemia). One of the efforts made as a treatment and prevention of DM is through complementary therapy using natural ingredients. Parijoto fruit (Medinilla speciosa) contains flavonoid compounds potential to reduce glucose levels. The study aimed to analyze the effectiveness of ethanol extracts and its fractions of parijoto fruit (EEBP) to decrease glucose levels in vitro. The study was conducted at the Ngudi Waluyo University Laboratory in April to August 2018. Identification and screening of flavonoid compounds from ethanol extracts and its fractions of parijoto fruit were performed qualitatively and by thin-layer chromatography (TLC). In vitro testing for  antidiabetic activity of EEBP was performed with non-enzymatic reaction by Nelson Somogyi method (ethanol extract and its fractions). Experiment  was arranged in a randomized block design, with 12 treatments and three replications. The concentrations of parijoto fruit tested were 10 ppm, 20 ppm, 30 ppm, 40 ppm, 50 ppm, and 60 ppm. The results of qualitative identification and TLC showed that the ethanol extract and its fraction of EEBP contained flavonoid compounds. At a concentration of 30 ppm, EEBP was able to reduce glucose levels by 42.43 %, while the ethanol fraction decreased glucose levels by 83.38 %. The results showed parijoto fruit ethanol extract and its fractions were sources of antidiabetic. Hence its pharmacologically effect was necessary to be further studied.","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47600071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Potential Isolates of Rhizosphere Bacteria to Contro Budok Disease in Patchouli Plant","authors":"N. Sukamto, Novi Listiana, R. Indrayanti, D. Wahyuno","doi":"10.21082/bullittro.v30n1.2019.35-46","DOIUrl":"https://doi.org/10.21082/bullittro.v30n1.2019.35-46","url":null,"abstract":"Synchytrium pogostemonis, the causal agent of budok disease, is one of the major limiting factors in patchouli production in Indonesia. Patchouli farmers usually control budok disease with chemical fungicides. Chemical control pollutes environment and disrupts agricultural ecosystem. Therefore, an environmentally friendly pest control should be conducted to control the disease. The objective of the study was to isolate and evaluate some rhizobacteria from the rhizosphere of patchouli and black pepper plants against Fusarium oxysporum f.sp. vanillae, F. solani, Sclerotium rolfsii. Potential rhizobacterial isolates were tested to control budok disease on a pot scale. The results showed that 26 rhizobacterial isolates from 100 tested were antagonistic to F. oxysporum, F. solani and S. rolfsii. Four rhizobacteria isolates (RL13-A, RL31-A, RL35-A, RL32-A) showed strong inhibition (>40 %) against the 3 pathogens. In polibag experiment, RL35-A, PS9, RL13-A, RL32-B, RL31-A isolates were able to suppress budok disease significantly by 84.01; 76.00; 65.99; 43.99; and 21.98 % respectively. These results indicated that RL35-A isolates have strong antagonistic effect compared to other isolates. Based on 16S rDNA analysis, RL35-A isolates possessed close relationship (99 %) with all species of Enterobacter sp. The antibiotic compound extracted from RL35-A culture broth using GC-MS analysis was identified as phenol, 2,6-dimethoxy-(canola). These results suggested that Enterobacter sp. was potential to be developed as biological agent for controlling budok disease in patchouli plants.","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48931251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Synergy Effect of Azadirachtin and Minor Components of Neem Seed Oil on Antifeedant Activity of Spodoptera litura","authors":"A. H. Prianto, Nfn Budiawan, Y. Yulizar, P. Simanjuntak","doi":"10.21082/bullittro.v30n1.2019.27-34","DOIUrl":"https://doi.org/10.21082/bullittro.v30n1.2019.27-34","url":null,"abstract":"Azadirachtin in neem oil ( Azadirachta indica A.Juss ) might have potential synergism effect with a minor compound in the neem oil as an antifeedant. This study aimed to determine the content of minor components that have the most significant contribution to the effect of synergy with the main component (azadirachtin) in antifeedant activity against Spodoptera litura . Neem oil was fractionated with n-hexane, ethyl acetate, and water. The antifeedant activity of three neem oil fractions was evaluated using the 4 th instar of S. litura larvae. The bioassay was conducted using the choice leaf disc test with 2.5; 5; 10; and 20 % (v/v) concentrations of each fraction. The concentration of the fractions showed equal azadirachtin content, hence the antifeedant activity represented the effect of the minor component in the fraction. The best activity was shown by water fraction (84-100 %). Probit analysis of the antifeedant activity of all fractions showed that water fraction has the best effective inhibitory (EI 50 ) at 1.0 %.  The minor component in the water fraction was dominated by phenol compounds (48.5 %) which were suspected to synergize with the main component (azadirachtin) in increasing the antifeedant activity on S. litura . These results indicated the phenol compound in this fraction has the role in increasing azadirachtin antifeedant activity against S. litura .","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48733087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Pyrethrum, Neem, Beauveria bassiana and Metarhizium anisopliae Against Brown Plant hopper (Nilaparvata lugens Stal.)","authors":"A. Kardinan, T. Wahyono, N. Tarigan","doi":"10.21082/bullittro.v30n1.2019.1-10","DOIUrl":"https://doi.org/10.21082/bullittro.v30n1.2019.1-10","url":null,"abstract":"Brown plant hopper (Nilaparvata lugens Stal) is the main pest on rice cultivation.  Synthetic insecticides application had negative impact to the human health and environment. The research objective was aimed to examine the effectiveness of botanical (pyrethrum and neem) and bio-insecticides (Beauveria bassiana/Bb and Metarhizium anisopliae/Ma) against brown plant hopper. Research was conducted at Entomology Laboratory of Indonesian Spices and Medicinal Crops Research Institute, Bogor in 2017. Trial consisted of two activities: the effectiveness of botanical and bio-insecticides to brown plant hopper mortality and horizontal transmission of bio-insecticides on brown plant hoppers. Botanical pesticide tested was (1) pyrethrum I (5 ml.l-1 water ), (2) pyrethrum II (5 ml.l-1 water), (3) neem I (20 ml.l-1 water), (4) neem II (20 ml.l-1 water), (5) synthetic insecticide (2 ml.l-1 water) and (6) control.  Bio-insecticide treatments were (1) Bb (spraying, 2.5 ml/plant), (2) Bb (granule, 5 g/pot), (3) Ma (spraying, 2.5 ml/plant), (4) Ma (granule, 5 g/ pot) and (5) control.  Treatments of horizontal transmission was the ratio of infected : healthy brown plant hopper 1 : 10; 2 : 10; 3 : 10 and 4 : 10. Botanical insecticides were prospective to suppressing brown plant hopper population of 85-87 % (pyrethrum) and   60-70 % (neem).   B.  bassiana was able  to   suppress   brown   plant     hopper population (18.2%), better than   M. anisopliae (5.6 %). Biological insecticide application by contact (spraying) was better than applied in granules form.  Botanical insecticide application showed no horizontal transmission from infected to healthy insect. Botanical insecticide (pyrethrum and neem) was more prospective than bio-insecticide (B. bassiana and M. anisopliae) in controlling brown plant hopper. ","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42971301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANALISA KELAYAKAN FINANSIAL PRODUKSI BIOPESTISIDA DARI BIJI MIMBA","authors":"Dwi Ajias Pramasari, A. Prianto","doi":"10.21082/bullittro.v30n1.2019.11-26","DOIUrl":"https://doi.org/10.21082/bullittro.v30n1.2019.11-26","url":null,"abstract":"Neem seed is a potential material for biopesticides, due to its limnoid content in the oil-seed. One of the methods for extracting neem seed oil is using screw press. The study aimed to determine the financial feasibility of neem seed based-biopesticides production for industrial-scale using flow chart of production approach. The tools used in this research was the financial feasibility approach such as NPV, IRR, Payback Period and Profitability Index. The financial feasibility study showed that biopesticides production was obtained at NPV Rp 3,026,193,872.00, IRR 46.90 %, Payback Period at 2 years 1 month, and Profitability Index at 2.40. The result of financial feasibility indicated that the biopesticides production using a screw press method at an industrial scale was feasible to be done. Sensitivity analysis showed that fluctuation of raw material cost and price of biopesticides product, affected the feasibility of neem seed based-biopesticides production using screw press method. This feasibility study is expected to be used as an initial reference for developing the potential of neem seed as biopesticides.","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67796789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Variability of Crossbreed-cashew Accession Numbers at Two Years Age Based on Morphological Characters","authors":"W. Haryudin, O. Rostiana, J. Darajat","doi":"10.21082/BULLITTRO.V29N2.2018.79-92","DOIUrl":"https://doi.org/10.21082/BULLITTRO.V29N2.2018.79-92","url":null,"abstract":"Cashew in cross-pollinated plants, one effort to improve the diversity gentik in germplasm of cashew nut was carried out by a cross between high-producing elders  and the elderly tolerant of helopeltis sp. The aimed of this study is to know the variability of 25 accession of cashew nut hybrid based on leaf morphology characters. The study was conducted at Cikampek Experimental Station, from January to December 2016, using direct observation method  the qualitative and quantitative morphological characters of the cashew at the age of two years. Observations were done on eight plants per plot, each observed as many as 50 leaves per plant. The result showed that the morphology character of cashew nut varied. Character of leaf ovatus, obovatus, and oblong The trunk and rounded ends, pointed and blunt. Form the edge of the leaf, form the bottom and top surfaces smooth leaves. The color of the adult leaves was dark green, and young leaves were reddish brown and  yellowish  green.  The  diversity  18.35-100 %  and  the level the closeness of 0.10-0.38, divided into two groups. Group one separated by characters a leaf shape  oblongus,  leaf  base  form  of the rotundatus, leaf tip shape rotundatus and obtusus and young leaves color BGB N199 A, group two separated by the characters of the leaf shape obovate, leaf base form of the obtusus, leaf tip shape acuminatus and retusus and young leaves color GB 200 B. The lenght of the leaf characters, the width of the leaf, the leaf thickness and the length of the petiole vary with the degree of diversity 47.67-96.94 % and the proximity distance 0.19-6.19 which is divided into teo groups. One group was separated by the the highest leaf length character 17.6-20.6 cm, whereas the two group was separated by the smallest leaf length character 14.6-17.1 cm.","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44616826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Rhizome Type to Viability, Growth, and Productivity of Three Turmeric Varieties","authors":"N. L. W. Meilawati, Nfn Melati, D. Rusmin","doi":"10.21082/bullittro.v29n2.2018.101-109","DOIUrl":"https://doi.org/10.21082/bullittro.v29n2.2018.101-109","url":null,"abstract":"Turmeric development to fulfil market demand as raw material for herbal industry requires qualified planting material in adequate quantities. This study aimed to evaluate the effect of varieties and seeds type of turmeric to the seeds efficiency, seeds viability, plant performance and rhizome yield. This experiment was conducted at the greenhouse of Indonesian Spices and Medicinal Crops Research Institute (ISMCRI), Bogor and Cicurug Research Installation, Sukabumi from November 2016 to July 2017. The study was arranged in split plot with 3 replications. The main plot was three varieties of turmeric: Curdonia, Turina 2 and Turina 3. The subplot was eight types of seeds-rhizome: (a) single finger rhizome, (b) finger rhizome with one branch, (c) finger rhizome with >1 branches, (d) mother rhizome split 2 horizontally, (e) mother rhizome split 2 vertically, (f) mother rhizome split 4 horizontally, (g) mother rhizome split 4 vertically and (h) whole mother rhizome. Parameters measured were seeds viability, growth and rhizomes yield. Seeds efficiency of Curdonia and Turina 2 can be achieved by using finger rhizome of about 0.49 t.ha-1 for both varieties,  rhizome yields of 6.63 t.ha-1 and 11.45 t.ha-1 respectively. Another alternative were the use of 0.47 t.ha-1 mother rhizome split 4 vertically, yielding 9.65 t.ha-1 and 14.20 t.ha-1 fresh rhizome for Curdonia and Turina 2 varieties, consecutively. However, for Turina 3 variety, the seeds efficiency was shown by using mother rhizome split 4 horizontally of 0.66 t.ha1 and yielding fresh rhizome  of 17.44 t.ha-1. ","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42850621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Response of Citronella Grass on Several NPKMg Fertlization Levels in Latosol Soil Type","authors":"Nfn Setiawan, Nfn Gusmaini, H. Nurhayati","doi":"10.21082/BULLITTRO.V29N2.2018.69-78","DOIUrl":"https://doi.org/10.21082/BULLITTRO.V29N2.2018.69-78","url":null,"abstract":"<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\"><tbody><tr><td valign=\"top\" width=\"456\"><p><em>Citronella grass (</em>Cymbopogon nardus var genuinus<em> L) is an essential oil-producing plant known as \"Citronella Oil of Java\". Oil is </em><em>resulted</em><em> from leaf distillation. The fertilization of N, P, K and Mg was expected to enhance herb productivity and oil yield of citronella grass. The research was conducted at Cicurug Experimental Garden, Sukabumi from August 2016 - February 2017. The objective of this research was to determine the proper dosage of NPKMg fertilizer on citronella grass in Latosol</em><em> soil type</em><em>. The study was arranged in a Randomized Block Design with 6 fertilization treatments and 4 replications. Fertilizer treatments were (1) without fertilizer (control), (2) NPKMg (12:12:17:2): (2) at the dosage of 141 kg.ha^-1,(3) 281 kg.ha^-1, (4) 421 kg.ha^-1, (5) 526 kg.ha^-1, and (6) Urea 150 kg.ha^-1, SP-36         50 kg.ha^-1 and KCl 125 kg.ha^-1 (as comparison). The NPKMg fertilization on citronella grass enhanced plant growth and increased the productivity of biomass and oil yield.  The application of NPKMg fertilization (12:12:17:2) 141 kg.ha^-1 produced 23 tons of herbs, increased by 30.8 % compared to NPK fertilization without Mg. The dosage of NPKMg fertilizer (12-12-17-2) at 281 kg.ha^-1 indicated the best growth and the highest herb production (24 ton.ha^-1</em><em>),</em><em> increased by 39 %, while the highest oil content (1.4 %) was shown by 421 kg.ha^-1NPKMg (12-12-17-2) <em>treatment, although the oil yield was insignificant compared to 281 kg.ha^-1. Thus, the dosage of 281 kg.ha^-1NPKMg (12:12:17:2) can be recommended for citronella <ins cite=\"mailto:ASUS\" datetime=\"2019-06-17T09:10\"> grass</ins> cultivated at Latosol<ins cite=\"mailto:ASUS\" datetime=\"2019-06-17T09:10\"> soil type</ins></em></em></p></td></tr></tbody></table>","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41873695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity of Stem Bark Ethanolic Extracts of Calophyllum pulcherrimum, C. soulattri, and C. teysmannii","authors":"E. Septiana, P. Simanjuntak","doi":"10.21082/BULLITTRO.V29N2.2018.59-68","DOIUrl":"https://doi.org/10.21082/BULLITTRO.V29N2.2018.59-68","url":null,"abstract":"The decreased air quality due to severe pollution causes the formation of free radicals in the body. Antioxidants counteract free radicals compounds and are beneficial to health. Calophyllum plants is one of the natural antioxidants source. The study was aimed to determine the best antioxidant activity of ethanolic extracts of stem bark both single and combinations of three species of Calophyllum . The study was conducted from September to December 2017 at the Natural Product Chemistry Laboratory, Research Center for Biotechnology LIPI, Cibinong, West Java. The experiment consisted of seven treatments, repeated three times.  The treatments consisted of ethanolic stem bark extracts of a) C. pulcherrimum b) C. soulattri (CS), c) C. teysmannii (CT), d) combination of C. pulcherrimum and C. soulattri (CP+CS), e) combination of C. pulcherrimum and C. teysmannii (CP+CT), f) combination of C. soulattri and C. teysmannii (CS+CT), and g) combination of C. pulcherrimum , C. soulattri and C. teysmannii (CP+CS+CT) with the ratio of mixed extract was 1 : 1 for treatments with two combinations and 1 : 1 : 1 for three combinations. The antioxidant activity was evaluated using DPPH free radical scavenging  methods.  The  total  phenolic   and   flavonoids   content   assays   were conducted base on the reaction of follin-ciocalteu and aluminum chloride reagents respectively.  The results showed that combination of Calophyllum stem bark ethanolic extracts revealed higher antioxidant activity, total phenol and flavonoid content compared to its sole extract. CP+CS+CT treatment has the lowest IC 50 value at 3.12 mg.l -1 so that it has the potential as a source of natural antioxidants.","PeriodicalId":32447,"journal":{"name":"Buletin Penelitian Tanaman Rempah dan Obat","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42151747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}