The Synergy Effect of Azadirachtin and Minor Components of Neem Seed Oil on Antifeedant Activity of Spodoptera litura

Buletin Penelitian Tanaman Rempah dan Obat Pub Date : 2019-10-28 DOI:10.21082/bullittro.v30n1.2019.27-34

A. H. Prianto, Nfn Budiawan, Y. Yulizar, P. Simanjuntak

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引用次数: 6

Abstract

Azadirachtin in neem oil ( Azadirachta indica A.Juss ) might have potential synergism effect with a minor compound in the neem oil as an antifeedant. This study aimed to determine the content of minor components that have the most significant contribution to the effect of synergy with the main component (azadirachtin) in antifeedant activity against Spodoptera litura . Neem oil was fractionated with n-hexane, ethyl acetate, and water. The antifeedant activity of three neem oil fractions was evaluated using the 4 th instar of S. litura larvae. The bioassay was conducted using the choice leaf disc test with 2.5; 5; 10; and 20 % (v/v) concentrations of each fraction. The concentration of the fractions showed equal azadirachtin content, hence the antifeedant activity represented the effect of the minor component in the fraction. The best activity was shown by water fraction (84-100 %). Probit analysis of the antifeedant activity of all fractions showed that water fraction has the best effective inhibitory (EI 50 ) at 1.0 %. The minor component in the water fraction was dominated by phenol compounds (48.5 %) which were suspected to synergize with the main component (azadirachtin) in increasing the antifeedant activity on S. litura . These results indicated the phenol compound in this fraction has the role in increasing azadirachtin antifeedant activity against S. litura .

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印楝素与印楝籽油微量成分对斜纹夜蛾拒食活性的协同作用

印楝油中的印楝素（Azadirachta indica A.Juss）可能与印楝油的一种微量化合物具有潜在的增效作用。本研究旨在确定在对斜纹夜蛾的拒食活性中，对与主要成分（印楝素）的协同作用贡献最大的次要成分的含量。用正己烷、乙酸乙酯和水对印楝油进行分馏。以斜纹夜蛾4龄幼虫为试材，对三种印楝油组分的拒食活性进行了评价。生物测定采用2.5；5.10；以及每个级分的20%（v/v）浓度。组分的浓度显示出相等的印楝素含量，因此拒食活性代表了组分中次要成分的影响。活性最好的是水含量（84-100%）。对所有组分的拒食活性进行了探针分析，结果表明，水组分的有效抑制率（EI 50）为1.0%。水分中的次要成分主要是酚类化合物（48.5%），这些化合物被怀疑与主要成分（印楝素）协同提高了对斜纹夜蛾的拒食活性。这些结果表明，该馏分中的酚类化合物具有提高印楝素对斜纹夜蛾拒食活性的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Buletin Penelitian Tanaman Rempah dan Obat

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12 weeks