{"title":"Rapid Detection of Avian Influenza Virus H9 in broilers by using RT-PCR in Al-Qadisiyah Province","authors":"Ali Akbar Mahmoudi, N. Jasim","doi":"10.29079/VOL17ISS2ART508","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART508","url":null,"abstract":"This study was conducted to detect Avian Influenza Virus H9 in broilers chicken farms in Al-Qadisiyah Province. Tracheal swab from 30 infected chicken flocks located in different areas of Al-Qadisiyah Province were collected to make rapid Immunochromatography test for AIV by using rapid Immunochromatographic Influenza virus antigen One-Step Kit and tissue samples were collected from flocks which showed positive for rapid test to make rRTPCR. The revealed that twenty-one (70%) flocks were positive for AIV by rapid Immunochromatography test. The molecular detection of AIV showed 23(76.67%) out of 30 flocks were positive for AIV H9. In conclusion, the AIV was the most important cause of respiratory diseases at the time of study. Both tests were found rapid, efficient and specific to detect AIV, but rt RT-PCR was more sensitive and more specific.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44932496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the virucidal efficacy of commercial antiviral drugs against Lasota virus a surrogate for enveloped viruses","authors":"A. F. Ghanim, H. Thwiny","doi":"10.29079/VOL17ISS2ART520","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART520","url":null,"abstract":"Many antivirals are commonly used in Iraq’s poultry farms and there is controversy about effective of these commercial antiviral drugs. The aim of this study was tested individually for the effectiveness of these commercial antiviral drugs. Four kinds of commercial antiviral drugs including VIRUX®, TopAMD®, V8® and Licorice® was used in this study. Lasota virus was used as a surrogate for enveloped viruses. The following tests: Spot hemagglutination, Antigen Rapid NDV Ag Test Kit, Electron microscopy, qRTPCR and Egg inoculation in order to determine the effect of these antivirals on hemagglutinating activity, viral protein, viral morphology, virus titer and viral infectivity respectively. All antivirals had no toxic effect on the chicken embryos. All these antivirals had no effect on heamagglutination activity except Licorice. No antivirals changed nucleoprotein antigenicity of Lasota virus. All antivirals had no effect on the morphology of the virus except Licorice destroyed the viral morphology and decreased in viral spikes. Three of the four antiviral reduced the viral titer while Licorice complete degradation of viral RNA and prevent detection it by qRTPCR. The allantoic fluid harvested from inoculated eggs with the treated Lasota virus with antivirals showed a remarkable decrease in viral infectivity as following: TopAMP about 20%, V8 40%, and Virux 60% while Licorice showed a complete reduction of viral infectivity (100%). In conclusion, the Licorice revealed the best antiviral activity.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41557739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gene expression study of pathogenic hemolysin producing E. coli isolated from cattle by using reverse transcription real-time PCR","authors":"H. Nasser, G. Ismaeel, N. M. Majeed","doi":"10.29079/VOL17ISS2ART505","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART505","url":null,"abstract":"The main goal of current study is an investigation of detection and expression of hemolysin toxin in E.coli isolates in clinical veterinary infection cases included Mastitis milk, Abortion samples and diarrhea samples from cattle in Al-Diwaniyah city by using real time-polymerase chain reaction technique. Total samples that used in this study are (24) isolates, included (9) isolates from mastitis cases, (5) isolates from abortion cases, and (10) isolates have taken from Diarrhea cases in cattle. The total prevalence of hemolysin in E.coli isolates is 18/24 (75%), where the percentage of occurrence of hemolysin production E.coli isolates by using real-time PCR was 7/9 (77%), 5/5 (100%), and 6/10 (60%) in Mastitis milk, Abortion and Diarrhea cases in cattle respectively. In concluding, production of hemolysin toxin by E.coli isolates have contrast activity depend on the type of sample; the study found the abortion isolates have high gene expression followed by diarrhea isolates, then milk isolates.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45656589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toxic pathological effect of mitomycin-c on male reproductive organs in rat","authors":"H. Naji, K. G. Chelab, A. Rhyaf","doi":"10.29079/VOL17ISS2ART517","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART517","url":null,"abstract":"The aim of present study was detected the effect of Mitomycin-c on the histological structure on male reproductive organ. In this study used (24) healthy adult male rats were divided into three groups, each group includes (8) animals: the 1 toxic group was injected (6.5mg/kg) of Mitomycin-c subcutaneous (s/c) once daily, the 2ed therapeutic or low dose group was injected (3.25 mg/kg) of Mitomycin-c subcutaneous (s/c) once daily, while the 3 control group was injected physiological normal saline (0.9% NaCl2). The experimental period was (10) weeks. Animal, which were treated with Mitomycin-c along the period of experiment showed, sever pathological changes, particularly in testes and epididymis.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44261363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comparative study among using of Ketamine, Tramadol, and Paracetamol in three injectable anesthetic protocols for induction of general anesthesia in sheep","authors":"A. Jassim, T. A. Abid","doi":"10.29079/VOL17ISS2ART498","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART498","url":null,"abstract":"The study was designed to compare the efficacy of using three injectable drugs; Ketamine, Paracetamol, and Tramadol in three anesthetic protocols. Fifteen apparently healthy adult sheep of either sex weighing (25-30 kg) were utilized, and divided into three equal groups; G1; (K4P10) was given Ketamine 4mg/kg B.W + Paracetamol 10mg/kg B.W, G2; (K4T2) was given Ketamine 4mg/kg B.W + Tramadol 2mg/kg B.W, and G3; (K4T2P10) was given Ketamine 4mg/kg B.W + Tramadol 2mg/kg + Paracetamol 10mg/kg B.W. Drugs were given to the animal by IV injection for creating induction of anesthesia. The induction time, length of surgical anesthesia, recovery time, and degree of analgesia, degree of muscle relaxation, respiratory rate, heart rate and rectal temperature were recorded at 5 minutes before injection of drugs and consider as control readings. Readings were repeated at 5, 10, and 15 minutes after administration of anesthetic drugs. Results were displayed that anesthesia with (K4T2P10) protocol was better compared with the two other protocols as it produced reliable surgical anesthesia, good analgesia, muscle relaxation and short recovery time.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42369412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histological evaluation of camel colostrum extracts effects as a new biological therapy on auricular cartilage defect healing.","authors":"Amir I. Towfik","doi":"10.29079/VOL17ISS2ART497","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART497","url":null,"abstract":"The aim of the present study was to demonstrate the effect of camel colostrum extracts on auricular cartilage defect healing. Iraqi camel colostrum were collected post-parturition at 0, 6 hours, double centrifuged, filtrated and refrigerated until use. Fifteen albino rats were divided randomly into 3 groups;(TG1) treated with colostrum collected directly after birth, (TG2) treated with colostrum collected 6 hours post-parturition, and (TG3) treated with normal saline. Under routine surgical approach, holes were done in their ear cartilages. The diameter of the holes were measured daily for one week. Biopsies were taken at 1, 2, 3 weeks for histological evaluation of cartilages healing. (TG1) showed the superiority of histological evaluation by complete healing between the two edges of chondrocytes in the site of defect, profuse fibrosis with formation of new blood vessels and mild infiltration of inflammatory cells. The results demonstrated that, the camel colostrum extracts had a beneficial therapeutic effects on cartilage defects, which might regarded as a new biological therapy.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46091240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"General Control Nonderepressible 2 and ATF4 Direct Liver Genes during Asparaginase Treatment in Mice","authors":"Rana Jaber Tarish","doi":"10.29079/VOL17ISS2ART516","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART516","url":null,"abstract":"Asparaginase (ASNase) treatment results in synthesis of some factors such as ATF4. The eIF2-ATF4 pathway is essential for cell survival during amino acid starvation conditions. Activation of the AAR in liver requires the eIF2 kinase called general control nonderepressible 2 kinase (GCN2). To what extent activation of the GCN2-eIF2-AAR is mediated by ATF4 is unknown. Our objective and hypothesis are addressed in our aim to describe the liver response to ASNase in mice deleted for Atf4. RNA sequencing alongside complementary biochemical approaches were performed in the livers of mice treated with eight daily injections of ASNase or saline excipient. Cellular pathways examined in detail included the AAR. We discovered that global hepatic gene expression patterns in Atf4 knockout mice overlapped with Gcn2 knockout mice. Shared hepatic pathways or processes altered during ASNase included mTOR signaling, and xenobiotic metabolism. On the other hand, loss of Atf4 during ASNase uniquely altered gene expression signatures reflecting signaling via eIF2 and ER stress. This research provides insight into the importance of genetic background of patients in choosing ASNase as a treatment.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":"758 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41282121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of Leishmania donovani in blood of experimentally infected rats by quantitative real-time PCR","authors":"N. I. Jarad, G. A. Jasim","doi":"10.29079/VOL17ISS2ART511","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART511","url":null,"abstract":"","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47309051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histological and morphometric study of the skin appendages of local breed sheep ovis ovis","authors":"M. Abdul, N. M., A. B.","doi":"10.29079/VOL17ISS2ART513","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART513","url":null,"abstract":"The aim of this study is provide histological and morphometric data information to the skin appendages of the local breed sheep regarding to the individual variation in different animal species to use in another sciences. The study carried out on ten healthy ram skin and the samples were taken during autumn season from fourth different anatomical sites (ventral ear surfaces, neck, middle dorsum, tail.). The study revealed that there were a number of skin appendages including( wool follicles and skin glands Sebaceous and sweat glands), two types of wool follicles were observed in the dermis (reticular layer) of the skin of local breed sheep in the form of primary and secondary wool follicles, the wool follicles in sheep were considered as a compound type in which there were more than one wool fiber emerged from one pore on the skin surface. The follicles was arranged as groups consist mainly of three follicles (Trio), in addition to (Dio) and (Mono) arrangement, in this study we noticed the greater diameter of the primary and secondary wool follicles in the neck region and the deeper primary wool follicles present in neck region ,while the deeper secondary wool follicles present in middle dorsum. In respect to the sebaceous glands, the present results indicate that they attached with the both types of wool follicles in which they opened at the upper part of the wool follicles via short duct the larger sebaceous gland found in neck region all the sweat glands of the skin of sheep were of Apocrine type, these glands which have large secretory parts and narrow duct were accompanied the primary wool follicle but the bigger sweat gland present in middle dorsum.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44760158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pharmaceutical allergy pattern for antibacterial of some specified species of bacterial which diagnosed by vitek-2 technology isolated from the infection of the yolk sac in the meat breeders in Al-Diwaniyah","authors":"K. Fahed, A. Neamah","doi":"10.29079/VOL17ISS2ART495","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART495","url":null,"abstract":"For the first time in Iraq, the study was used electronic vitek-2 apparatus for detection some bacteria that were isolated from omphalitis cases of broiler chicks of meat breeders in AL-Diwaniyah city. Thirty swaps were taken from thirty of meat broiler chicks infected by yolk sac infection or omphalitis then the samples tested biochemical tests by use electronic vitek-2 apparatus after culture all the isolates on media. E.coli, Staphylococcus aureus, and Enterococcus faceuim were recorded by our study from the samples with percentages (60%), (23.3%) and (16.6%) respectively; then the antibiotics sensitivity test has done for all the isolates where the antibiotics that have more effect were trimethoprim(TR)(5MG), Amikacin(AK)(30mg) and Tetracycline(TE)(30mg) respectively. E.coli was prevalent from other bacteria, the subject was required more studies about all aspects like pathological, molecular, and immunological.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44995786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}