{"title":"Molecular and microscopic study of Entamoeba histolytica in Camels in Al-Qadisiyah & Al-Najaf Al-Ashraf Provinces","authors":"H. A. Bayati, G. A. Jasim","doi":"10.29079/VOL17ISS2ART512","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART512","url":null,"abstract":"Using a PCR technique, the detection of Entamoeba histolytica in camels was the main reason for performing of this study. Two hundred fecal samples of the camels from the slaughterhouses & nomadic areas of Al-Qadisiyah and Al-Najaf Al-Ashraf provinces. Some of camels suffered from diarrhea & other asymptomatic animals, their ages range from less than 1 year to more than 1 year. Whereby showed the prevalence of Entamoeba histolytica infection was (30.5%) by Lougal iodine stain method according to using microscopic examination for description of E. histolytica cysts. While the prevalence rate depending the PCR technique there was (61%).","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47342350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of some environmental stresses on nutrients concentration of Phragmites australis and use it as fodder","authors":"M. Guda","doi":"10.29079/VOL17ISS2ART509","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART509","url":null,"abstract":"This study has been conducted to assess and analyze the nature and environmental characteristics of Baher Al-Najaf depression (BAND) and their effect on nutrients Concentration of Phragmites australis as responses to Environmental Stresses and use it as fodder, has been studied for 12 month in four different sites. Samples were collected monthly for the period February, 2015 to January 2016, taken from four selected sites in (BAND), Iraq.The environmental factors of water studied include: Temperature, Electrical Conductivity (EC), Salinity, Water pH, Total Dissolved Solids (TDS), Turbidity, Total Suspended Solids (TSS), Dissolved Oxygen (DO), Total Hardness (TH), ions, Total Nitrogen (TN), Total Phosphorus (TP), Bicarbonate (HCO3 ), Sulfate (SO4 ), Chloride (Cl), and sodium (Na) ions. Results of water quality have proved the highest values of environmental factors were in autumn and summer while, least environmental factors were in winter and spring. Environmental factors were gradients significantly high at site as follows S2 > S3 > S4 > S1.The studied factors of climate are : Air Temperature, Relative Humidity, Intensity of Solar Radiation , Precipitation Rates, Day Length and Evaporation Rate. Results have shown that climatic factors affected water resources and salinity level in (BAND). The changes in the nutrient cycling and/or accumulation are in most cases unpredictable, and that is why detailed description of the local structure and state of within-stand development are necessary. The ability and translocation are important, especially in changing environments with varying supply of resources. Ionic contents Na and Cl significantly increased, whereas K, TP (Total Phosphor) and TN (Total Nitrogen) decreased with increasing levels of environmental stresses in each site and season. In general it can be use Phragmites australis as fodder in suitable environmental factors and it cannot be used as fodder in stress environmental factors due to decrease in nutrients concentration of Phragmites australis.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46529852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of beta vulgaris (beetroot) in hemoglobin (Hb) elevating in patients with Anemia due to iron deficiency","authors":"W. M. Kadhem, H. Enaya, Arshed S. kadhom","doi":"10.29079/VOL17ISS2ART518","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART518","url":null,"abstract":"The human life interesting is the main important of world health care. The plant family has vigorous of natural element and chemical compounds that can use greatly in medicine and other applications. This study involved used the beetroots for elevated the hemoglobin percentage in adult patients suffering with anemia that resulting from iron deficiency .Because the Beta vulgaris provides B-complex vitamins and minerals ex. potassium, copper and magnesium. This study sample composed of (34) patients who randomly distributed to three unequal groups (man – women – control).The results showed significant (P< 0.05) with RBC, Hb and PCV elevating in all patients and woman have increasing more than men.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42304406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. M. Karim, M. A. A. Rodhan, Khalefa Ali Mansou, Nawras Kadhum Mahdee, Saad Hashim Al-Husseiny
{"title":"Phylogenic analysis of the sheep Orf virus in Iraq","authors":"S. M. Karim, M. A. A. Rodhan, Khalefa Ali Mansou, Nawras Kadhum Mahdee, Saad Hashim Al-Husseiny","doi":"10.29079/VOL17ISS2ART507","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART507","url":null,"abstract":"Orf disease is a highly contagious, zoonotic viral skin disease affects sheep, goats and some other domesticated and wild ruminants, therefor this study is designed to evaluate predominant genotypes circulating Orf virus of sheep in Al-Qadisiyah Province in Iraq as the first time by using the sequencing and phylogenic analysis of isolates Orf virus strains. The study was carried out on (94) skin samples of affected sheep animals diagnosed previously by RT-PCR technique in different ages and sexes between September /2013– March/ 2014. PCR assay was performed for amplification of 408bp GM_CSF/IL-2 inhibition factor (GIF) gene Orf virus in positive samples of real-time PCR assay. Eight purified DNA products isolated from different areas of Al-Qadisiyah Province (one sample for each area) were analyzed by using sequencing method to obtain the nucleotide sets of GIF gene. DNA sequencing technique was performed for Phylogenic relationship analysis study of local Orf virus positive samples isolates with NCBI-GenBank Global Orf virus isolates. The results of endpoint polymerase chain reaction for detection of specific GIF gene (408 bp) of Orf virus showed positive results for all collected examined samples. Sequencing analysis of our isolates in the present study recorded and published under accession numbers were (KJ508895.1, KJ634611.1, KJ634842.1, KJ648451.1, KJ653445.1, KJ653446.1, KJ659373.1 and KJ697772.1). The results of phylogeny tree construction showed that five of our published isolates of this study (KJ508895.1, KJ648451.1, KJ653445.1, KJ653446.1, and KJ697772.1) are located in the same first tree branch, which shared highest and closed relationship with AF192803.1, DQ184476.1 Orf virus strain NZ2 isolated from New Zealand. The KJ659373.1 our published Iraqi Orf virus isolates of this study showed high homology and closed relationship with AY605973.1 Orf virus isolated from reindeer in Norway. Phylogenic analysis of KJ634611.1 and KJ634842.1 our published Iraqi Orf isolates of the present study showed high homology with AY605977.1 Orf virus isolated from goat in Norway. Sequencing study and phylogenic analysis have been considered useful in understanding Orf virus scenario in Iraqi endemic area and which important in application of control measures and selective efficient vaccines to use in vaccination programs in Iraq.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42946642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aflatoxins B1 and M1 residues in chicken's livers in Al Diwaniyah city","authors":"M. A. Molaghi, K. N. Taher, Falah H Abd Allatif","doi":"10.29079/VOL17ISS2ART510","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART510","url":null,"abstract":"In this study, (60) samples of local and imported chicken liver were collected with (30) samples of each type from markets of Al Diwaniyah city. High Performance Liquid Chromatography (HPLC) technique used to detect the amount and type of aflatoxin residues. The results of the local chicken liver test showed that B1 and M1 residues was observed in (17) samples of (30) samples tested (56.7%) and (13) (43.3%).Total of the two toxins was between (1.9 72.2) ppb at the rate of (21.15) ppb, and (5) samples (29.41%) of the local chicken liver is not suitable for human consumption to overcome the toxins in the alfatoxins where the limits allowed in human food. The results of the imported chicken liver examination showed that (15) samples of B1 and M1 were infected (30) samples (50%). The range of toxin amount of the total of the two types of toxins ranged between (0.5 52.2) ppb at rate (16.71) ppb, and the amount of toxins in (5) samples of the allowed limits, and the local chicken liver more contain the foals of aflatoxins chicken liver, but there were no significant differences between the two types.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43749727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative study of some indicator bacteria of sheep and goats raw milk in Al-Qadisiyah Province","authors":"K. H. Abbas, K. Taher, Falah H. Abidallatef","doi":"10.29079/VOL17ISS2ART499","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART499","url":null,"abstract":"The present study was carried out in Al-Diwaniyah city and its different districts, which includes (city center, Afak district and their regions, Al-Hamza district and their regions, AlShamiya district and their regions, Al-Daghra regions and its villages) during a period November 2009 to July 2010. A total of samples used in this study were 120 raw milk samples collected includes 60 samples for each species for (sheep and goats) for two different season of year (Winter and Summer), first start from (beginning November 2009 to end of the January 2010),and the second start from (beginning May to July 2010) The results of this study revealed the rising the averages of microbes contaminated raw milk samples in each species of animals included in this study where raw milk samples for sheep recorded (0.16×10 CFU/ml) and goats (0.13×10 CFU/ml). The highest averages of total Coliform bacterial count were found in raw milk samples of sheep and goats (0.15×10 CFU/ml). While averages of Staphylococcus aureas were high in raw milk samples of sheep (0.20×10 CFU/ml) and less in raw milk samples of goats (0.14×10 CFU/ml). While the average of Streptococcus spp. Were (0.30×10 CFU/ml) and (0.27×10 CFU/ml) for sheep and goats respectively. Seasons of the year had effect on average of total bacterial count studied for each samples of raw milk for all species of animals included in this study. Where shown rising averages of all microbes numbers during summer period while there are decreasing in average of microbes during winter period which variances significantly (P≤0.05).","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49129852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR","authors":"Jameela Radi Esmaeel, J. Sadeq","doi":"10.29079/VOL17ISS2ART504","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART504","url":null,"abstract":"Hemolysin protein is exotoxin produce by organisms that cause lysis of blood cells. This study was conducted to screen the presence of hemolysin gene from 20 isolates of Klebsiella pneumonia based 16S rRNA genes by using specific primer. This gene potent the pathogenesis of Klebsiella pneumonia. The primer was designed in this study by NCBI-GenBank and primer3 plus. (Bioneer Company provided the primers. Korea). Molecular detection of isolates, which give away specific PCR products of 505bp for hly gene, hemolysin gene, was detected in 70% (14/20).","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46154711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transcriptional profiling of livers from different strain of mice treated with Asparaginase","authors":"Rana Jaber Tarish","doi":"10.29079/VOL17ISS2ART515","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART515","url":null,"abstract":"Asparaginase (ASNase) is widely used to treat acute lymphoblastic leukemia (ALL) in children but it causes metabolic complications related to liver toxicity. ASNase results in synthesis of some factors such as ATF4. The eIF2-ATF4 pathway is essential for cell survival during amino acid starvation conditions. Activation of the AAR in liver requires the eIF2 kinase called general control nonderepressible 2 kinase (GCN2). To what extent activation of the GCN2-eIF2-AAR is mediated by ATF4 is unknown. Our objective and hypothesis are addressed in our aim to describe the liver response to ASNase in mice deleted for Atf4. RNA sequencing alongside complementary biochemical approaches were performed in the livers of mice treated with eight daily injections of ASNase or saline excipient. Differences in gene expression were evaluated. We also explored the relationship between the different treatment groups and strains. This research provides insight into the importance of genetic background of patients in choosing ASNase as a treatment.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44908478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rafid H. Farman, Saad Hashim Al-Husseiny, Ali Nair Abd Al Ameer
{"title":"Surgical treatment of hernia in cattle: A review","authors":"Rafid H. Farman, Saad Hashim Al-Husseiny, Ali Nair Abd Al Ameer","doi":"10.29079/VOL17ISS2ART506","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART506","url":null,"abstract":"This study aimed to highlight on hernial types that effect animals specially in cattle, the ways of diagnosis and treatment by using different techniques, which way is more suitable either the highlight on advantage and disadvantage of these techniques. There are many type of hernia may happen in cattle's but the most frequently noted in cattle was ventral and umbilical hernia. The researches confirmed that the umbilical hernia usually accrued in calves due to failure to close of the umbilical cord, while ventral hernia is usually present in cattle due to trauma, heavy weight. Hernia was easy to diagnoses by case history, inspection and palpation. There are several techniques to treat hernia, surgical or non-surgical depending on the size and the type of hernia. In case of surgical techniques, there are twotype open and closed surgical operation. The recent searches commend that in case of simple and small hernia in size they treat by suturing the edges of ring by absorbable suture (Hernioraphy), but in case of large hernia must be used mesh to support the muscle in technique named (Hernioplasty). Mesh may cause irritation in its site, cause post-operative pain, and delay the time of healing so the recent searches to make solution to this problems mesh must be covert by special material to make barrier between it and muscle and lubricated the site hernia.","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44845629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdularazzaq Baqer Kadhim, Eman I. Dali, H. A. Sharoot
{"title":"Histomorphological study of duodenum of goose (Anser anser)","authors":"Abdularazzaq Baqer Kadhim, Eman I. Dali, H. A. Sharoot","doi":"10.29079/VOL17ISS2ART503","DOIUrl":"https://doi.org/10.29079/VOL17ISS2ART503","url":null,"abstract":"The present work includes anatomical and histological studied of different part of the duodenum in the Goose (Anser anser). It was taken (10) birds were anesthesia and work of a longitudinal incision in the abdomen and the eradication of the gut complete manner and then duodenum measurements, Anatomical study shown duodenum divided into three parts ascending, descending and middle part. Histological study after dyeing in the form of hematoxylin and eosin represent wall of duodenum in all parts consist of four layer tunica mucosa, submucosa, muscularis and serosa. The tunica mucosa of all parts of duodenum was studied with folded villi of different shape and size, which were lined by simple columnar epithelium having more goblet cells in proximal part in contrast middle and distal part. The goblet cells are distributed between enterocytes and secrete mucus that covers the mucosa. The apical part of villi of proximal duodenum were slightly pointed and the basal parts of villi were thicker than middle and distal duodenum that’s come from the proximal part of duodenum plays major role in net nutrient absorption Lamina muscularis mucosa is various in thickness in all parts of duodenum and was made up of smooth muscle fibers. Tunica submucosa in duodenum of goose in this study consist of loose connective tissue rich with lymphoid fibers and duodenal glands. The musculosa layer consists of two smooth muscle layers outer longitudinal layer and a thick circular muscle layer that allow mixing and propulsion of the digesta through the intestinal tract","PeriodicalId":32194,"journal":{"name":"AlQadisiyah Journal of Veterinary Medicine Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49221417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}