Rapid Detection of Avian Influenza Virus H9 in broilers by using RT-PCR in Al-Qadisiyah Province

This study was conducted to detect Avian Influenza Virus H9 in broilers chicken farms in Al-Qadisiyah Province. Tracheal swab from 30 infected chicken flocks located in different areas of Al-Qadisiyah Province were collected to make rapid Immunochromatography test for AIV by using rapid Immunochromatographic Influenza virus antigen One-Step Kit and tissue samples were collected from flocks which showed positive for rapid test to make rRTPCR. The revealed that twenty-one (70%) flocks were positive for AIV by rapid Immunochromatography test. The molecular detection of AIV showed 23(76.67%) out of 30 flocks were positive for AIV H9. In conclusion, the AIV was the most important cause of respiratory diseases at the time of study. Both tests were found rapid, efficient and specific to detect AIV, but rt RT-PCR was more sensitive and more specific.