Food Control最新文献

{"title":"Gold nanoparticles loaded filter paper: A recyclable nanozyme for colorimetric determination of Hg2+ in tap water and beef","authors":"Melisew Tadele Alula ,&nbsp;Peter Lemmens ,&nbsp;Nicolette R. Hendricks-Leukes ,&nbsp;Tiny Motalaote ,&nbsp;Markus Etzkorn ,&nbsp;Lindsay Robin Wilson ,&nbsp;Felicity Babuseng","doi":"10.1016/j.foodcont.2025.111349","DOIUrl":"10.1016/j.foodcont.2025.111349","url":null,"abstract":"<div><div>Recently, gold nanoparticles (AuNPs) have been used as a potential nanozymes for the colorimetric determination of various target analytes. High cost as well as a stability problem of AuNPs, however, limit their application despite their high signal producing features to allow nanozymes-based detection. Strategizing a method to produce stable and recoverable AuNPs for repeated use reduces the cost and carbon footprint of these analyses. In this work, we used abundantly available cellulosic polymer, filter paper, as a support to anchor and stabilize the colloidal AuNPs. The deposition of AuNPs on the porous structure of the filter paper was facilitated by the hydrophilic and absorbing properties of cellulosic filter papers (circular punched). The resulting device consisting of gold nanoparticles deposited filter paper (FP@AuNPs) showed peroxidase-like activity and remained stable with no decreasing peroxidase-like activity for an extended period. The peroxidase-like activity was further enhanced by adding Hg²⁺ to the reaction system and hence a sensitive and selective colorimetric method for the determination of Hg²⁺ was developed. The LOD for analysis of Hg²⁺ was found to be 19.4 nM in a linear concentration range of 0.025–60 μM. The applicability of this method in analyses of real samples was evaluated using tap water and commercial beef samples. Excellent recovery rates showed the reliability of this method in the determination of Hg²⁺ in real samples. The nanozyme was recovered from a reaction mixture and reused for five cycles to detect Hg²⁺ without losing its nanozyme activity. The recurring nanozyme activity after repeated use shows the potential of this system to be an inexpensive recyclable detection system with low environmental carbon footprint. This is rather uncommon for recently developed nanozymes.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111349"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A versatile triple-channel fluorescent probe for quantitative detection of three preservative residues in mushroom products (bisulfite, daminozide and hypochlorite)","authors":"Jiawen Liu ,&nbsp;Wanqi Liu ,&nbsp;Zhiwei Sun ,&nbsp;Di Wu ,&nbsp;Yongning Wu ,&nbsp;Guoliang Li","doi":"10.1016/j.foodcont.2025.111346","DOIUrl":"10.1016/j.foodcont.2025.111346","url":null,"abstract":"<div><div>A triple-channel fluorescent probe (MPIM) was developed for real-time detection of bisulfite (HSO₃⁻), hypochlorite (ClO⁻) and unsym-dimethylhydrazine ((CH₃)₂NNH₂) residue in mushroom products with different preservative processing (sulfur dioxide fumigation, spray with daminozide, or chlorinated disinfection and canning). In an optimized solvent environment, MPIM exhibits distinguishable fluorescent responses toward HSO₃⁻, (CH₃)₂NNH₂ and ClO⁻. The probe MPIM itself emit bright orange-red fluorescence (λ_em = 626 nm), while it will change to purple fluorescent emission (λ_em = 389 nm) when exposed to HSO₃⁻, turn to blue fluorescence (λ_em = 436 nm) when exposed to (CH₃)₂NNH₂, and covert to green emission (λ_em = 490 nm) when in contact with ClO⁻. These transformation of fluorescent signals can be directly observed by the naked eye. At the same time, MPIM showed excellent low detection limit for HSO₃⁻, (CH₃)₂NNH₂ and ClO⁻ (the detection limits were 19 nM, 17.3 nM and 7.9 nM, respectively). The probe MPIM has been successfully applied for detecting HSO₃⁻, (CH₃)₂NNH₂ or ClO⁻ in three kinds of actual mushroom products. Moreover, an intelligent detection sensor platform is constructed by combining the MPIM loaded strips with smart phone RGB analysis software. The intelligent detection platform shows satisfactory chroma change results. Therefore, the successful construction of MPIM is expected to develop into an effective tool for assessing illegal or excessive addition of these preservatives in mushroom samples, which has a exciting prospect in the field of food safety detection in the future.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111346"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative food safety strategy: Eugenol nanoemulsion with lactobacillus derived post-biotic biopolymer for biofilm inhibition on food and contact surfaces","authors":"Sangeeta Balyan ,&nbsp;Vikas Dadwal ,&nbsp;Deepak Kumar Jha ,&nbsp;Bhimanagouda S. Patil","doi":"10.1016/j.foodcont.2025.111348","DOIUrl":"10.1016/j.foodcont.2025.111348","url":null,"abstract":"<div><div>Foodborne pathogens such as <em>Listeria monocytogenes</em> and <em>Salmonella</em> spp. form resilient biofilms on food-contact surfaces and fresh produce, posing a significant challenge to food safety. This study is the first of its kind to investigate a postbiotic biopolymer-based eugenol nanoemulsion for biofilm control in food safety applications. The biopolymer, an exopolysaccharide (EPS) derived from <em>Lactobacillus plantarum</em>, was utilized as a natural emulsifier to encapsulate eugenol, enhancing its stability and antimicrobial efficacy. The Eugenol -EPS (Eu-EPS) nanoemulsion exhibited an average droplet size of 192 ± 1.89 nm and a zeta potential of −32 ± 1.90 mV, ensuring nanoscale dispersion and colloidal stability. The encapsulation efficiency was measured as 65.23 ± 0.45 %. The nanoemulsion demonstrated a bacteriostatic effect, achieving up to 3.17 log CFU/cm² biofilm reduction on stainless steel and plastic surfaces (<em>p &lt; 0.05</em>). On fresh produce, it led up to a 3-log reduction of <em>Salmonella</em> and <em>Escherichia. coli</em> on tomatoes and blueberries, and a 4-log reduction in <em>L. monocytogenes</em> on queso fresco cheese, highlighting its potential as a natural and food-safe antimicrobial solution. By utilizing lactic acid bacteria EPS as a functional carrier for eugenol nanoemulsions, this study introduces a sustainable biofilm control strategy as an alternative to conventional chemical sanitizers in food processing environments.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111348"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-bacterial and anti-biofilm effect of punicalagin against Pseudomonas fluorescens and its application in reducing spoilage of tilapia fillets induced by Pseudomonas fluorescens","authors":"Chanchiang Liang,&nbsp;Siyang Chen,&nbsp;Wenxiu Zhu,&nbsp;Hanzheng Dou,&nbsp;Xiaodong Xia","doi":"10.1016/j.foodcont.2025.111345","DOIUrl":"10.1016/j.foodcont.2025.111345","url":null,"abstract":"<div><div>The antimicrobial efficacy of punicalagin against foodborne pathogens has been extensively studied, yet its impact on spoilage microorganisms remains poorly characterized. This study aimed to evaluate punicalagin's inhibitory effects on <em>Pseudomonas fluorescens</em> and explore its potential application in tilapia preservation. Growth curve analysis showend that punicalagin effetively inhibited the growth of <em>Pseudomonas fluorescens</em>. In addition, punicalagin triggered intracellular potassium ion efflux and leakage of nucleic acids and protein. Scanning electron microscopy revealed compromised cell membrane integrity after punicalagin treatment. Furthermore, punicalagin exposure reduced intracellular ATP levels and elevated reactive oxygen species accumulation. Concurrently, it effectively suppressed biofilm formation and destroyed preformed biofilms. In tilapia spiked with <em>Pseudomonas fluorescens</em>, punicalagin markedly attenuated spoilage progression, as evidenced by diminished protein degradation and lipid oxidation. These collective findings indicate that punicalagin exhibit antimicrobial and anti-biofilm activities against <em>Pseudomonas fluorescens</em>, highlighting its potential as a natural preservative for aquatic products.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111345"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hollow calcium carbonate microspheres prepared from waste eggshells as pH-responsive controlled-release carvacrol for efficient and safe antimicrobial agents in pork preservation","authors":"Xiaomiao Chen ,&nbsp;Dankui Liao ,&nbsp;Qian Zhou,&nbsp;Xiaofang Li,&nbsp;Jianhua Sun,&nbsp;Zhangfa Tong,&nbsp;Liting Lai,&nbsp;Lixia Sun,&nbsp;Guangzhi Zhou","doi":"10.1016/j.foodcont.2025.111347","DOIUrl":"10.1016/j.foodcont.2025.111347","url":null,"abstract":"<div><div>The application of safe controlled-release antimicrobial agents can significantly enhance the storage and preservation of meat, providing substantial commercial value. This study utilized waste eggshells to prepare CaCO₃ hollow microspheres loaded with carvacrol (CA) to enhance the bactericidal effect of carvacrol (microSCaCA), forming a pH-responsive controlled-release antimicrobial agent. The release performance and antimicrobial effects were investigated, and after ensuring cellular non-toxicity, the preservative effects on refrigerated pork were examined. Results showed that the CaCO₃ hollow microspheres could load up to 86.20 mg/g of CA, with improved release performance at lower pH values. In a PBS solution at pH 5.0, the cumulative release rate of carvacrol reached 65.73 % within 60 h, approximately 4.65 times higher than in neutral pH conditions. MicroSCaCA significantly inhibited <em>E</em>. <em>coli</em> and <em>S</em>. <em>aureus</em> and demonstrated good biosafety in cytotoxicity tests. When applied to the preservation of pork at 4 °C, microSCaCA significantly improved preservation indicators and extended the shelf life of fresh meat by 4 days. This study not only enhanced the application effectiveness of carvacrol through waste utilization but also provided a new pathway for the antimicrobial use of plant essential oils, demonstrating the potential application value of CaCO₃ hollow microspheres in antimicrobial and food preservation applications.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111347"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel analytical data on cadmium, mercury, lead, arsenic and selenium content in fish from Tanzania and Mozambique: Implications for food safety","authors":"Talhiya Maulid Ali ,&nbsp;Daniel Shilla ,&nbsp;Haji Mwevura ,&nbsp;Mackrina Patrick Nombo ,&nbsp;Edel O. Elvevoll ,&nbsp;Maria Wik Markhus ,&nbsp;Quang Tri Ho ,&nbsp;Henriques Bustani ,&nbsp;Marian Kjellevold","doi":"10.1016/j.foodcont.2025.111350","DOIUrl":"10.1016/j.foodcont.2025.111350","url":null,"abstract":"<div><div>Fish are important for food security and nutrition in many coastal communities; however, fish consumption is also a major source of metal and metalloid exposure to humans. Data on metal/metalloid content in fish from Tanzania and Mozambique marine waters are limited. This study analysed the content and assessed the risk of exposure from 17 fish species sampled off the coast of Tanzania and Mozambique by research vessel Dr. Fridtjof Nansen during an ecosystem survey in 2018. The samples were prepared according to local consumption habits (fillet, whole and dressed) and were analysed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Three metals, cadmium (Cd), mercury (Hg) and lead (Pb), and two metalloids, arsenic (As) and selenium (Se), were measured. The content varied among the species and tissue analysed. Hg content was higher in fillets from large predatory fish, while As, Se, Cd and Pb were higher in small fish that traditionally are consumed whole. The mean Se-Hg molar ratio was higher in whole and dressed small fish compared to fillets from large fish. In all analysed samples, HQ_Cd and HQ_Hg values were &lt;1 except for <em>O. bartramii</em>, where HQ_Cd was marginally above 1. Thus, the consumption of the fish species, prepared to resemble the eating habits collected in this study, has no adverse health effects on humans, and the consumption of whole fish is highly recommended from a nutritional point of view. However, species diversification and the use of safe consumption limits may also be important in reducing multiple contaminant exposure.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111350"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the prevalence of Paralytic Shellfish Toxins in non-traditional vectors and potential health risks associated to their consumption","authors":"Verónica Rey ,&nbsp;Araceli E. Rossignoli ,&nbsp;Francisco Rodríguez ,&nbsp;Juan Blanco ,&nbsp;Soledad Garrido ,&nbsp;Begoña Ben-Gigirey","doi":"10.1016/j.foodcont.2025.111351","DOIUrl":"10.1016/j.foodcont.2025.111351","url":null,"abstract":"<div><div>Paralytic shellfish toxins (PSTs) are potent marine neurotoxins mainly associated with dinoflagellates. European Union (EU) Regulation Nº 853/2004 and its amendments establish the maximum permitted levels for PSTs in bivalve molluscs, echinoderms, tunicates and gastropods. In Europe, the analyses of marine biotoxins for monitoring purposes is mainly focused on bivalve molluscs. Other vectors, including some from the regulated groups, are rarely covered by monitoring programs.</div><div>The aim of this study is to estimate the prevalence of PSTs across species from eight marine invertebrate groups and fish in Galicia (NW Spain). In total, 533 invertebrates (echinoderms, gastropods, cnidarians, crustaceans, cephalopods, polychaetes, tunicates, porifera and bivalves) and 96 fish samples were collected between April 2021 and September 2023 and analysed using both LC-FLD and LC-MS/MS methods. PSTs were detected in 265 samples in all groups except cephalopods and tunicates. Quantifiable levels were found in 233 samples.</div><div>Our results show the existence of potential PST vectors other than bivalves, including edible organisms of great economic value. These findings could be extrapolated to other countries with similar harmful algae events and stress the need for further surveys on different groups of marine invertebrates for a better public health protection. To our knowledge, this is the first report of PSTs in <em>Littorina</em> spp., <em>Nassarius</em> spp., <em>Patella ulyssiponensis</em>, <em>Balanus</em> spp., <em>Carcinus maenas</em>, <em>Luidia sarsi</em> and <em>Ophiotrix</em> spp. in the EU. Moreover, PSTs are also described for the first time in <em>Nucella</em> spp., <em>Patella</em> spp. and <em>Polybius henslowii</em> from Spain.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"176 ","pages":"Article 111351"},"PeriodicalIF":5.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric fluorescence biosensor based on enzyme-cascade signal amplification technology for the detection of lincomycin","authors":"Yiyao Liu,&nbsp;Menglong Liu,&nbsp;Jingyi Xiao,&nbsp;Yi Ren,&nbsp;Jinyang Li,&nbsp;Jiayan Gao,&nbsp;Litao Chen,&nbsp;Xue Gao","doi":"10.1016/j.foodcont.2025.111344","DOIUrl":"10.1016/j.foodcont.2025.111344","url":null,"abstract":"<div><div>An enzyme-nanozyme cascade amplified ratio fluorescence (RF) biosensor based on prepared MnFe-layered double hydroxide nanosheets (Mn-Fe LDH) and alkaline phosphatase (ALP) has been developed for sensitive and accurate detection of lincomycin (LIN). In this strategy, Mn-Fe LDH can catalyze the o-phenylenediamine (OPD) oxidation to generate fluorescence 2, 3-diaminophenazine (DAP) with a fluorescence wavelength of 565 nm. This study utilized LIN aptamer-modified magnetic beads and alkaline phosphatase-labeled complementary strand hybridization to prepare the MBs-Apt@cDNA-ALP. The MBs-Apt@cDNA-ALP could recognize LIN and release cDNA-ALP from the surface of the magnetic beads. After magnetic separation, cDNA-ALP catalyzed the 2-phosphate ascorbic acid (AAP) conversion to ascorbic acid (AA). The redox reaction between AA and Mn-Fe LDH led to a decrease in DAP production and fluorescence intensity. At the same time, AA converted into dehydroascorbic acid (DHAA), which reacted with OPD to form a quinoline derivative (DFQ) that emitted fluorescence at 435 nm. The constructed LIN biosensor exhibited a detection range of 1.125–250 nM with a limit of detection (LOD) of 0.0492 nM. Moreover, the RF aptasensor can eliminate background interference, deliver remarkable signal variations, and effectively analyze the LIN concentration in grass carp and shrimp samples.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"175 ","pages":"Article 111344"},"PeriodicalIF":5.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143806902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inactivation of Penicillium spp. in apple juice by pulsed light and the exploration of its mechanisms","authors":"Guolong Li ,&nbsp;Saiqun Wang ,&nbsp;Yu Li ,&nbsp;Lu Cui ,&nbsp;Rui Cai ,&nbsp;Tianli Yue ,&nbsp;Yahong Yuan ,&nbsp;Zhouli Wang","doi":"10.1016/j.foodcont.2025.111343","DOIUrl":"10.1016/j.foodcont.2025.111343","url":null,"abstract":"<div><div><em>Penicillium</em> spp. is a crucial factor affecting fruit juice quality, so it is imperative to control their contamination. The objective of this study was to evaluate the efficiency of pulsed light (PL) treatments on the inactivation of <em>Penicillium</em> spp. in apple juice and its mechanisms. The effect of different processing parameters on the inactivation of <em>Penicillium</em> spp. was explored. After inoculation of 6.00 log CFU/mL of target fungi in 12 ^oBrix apple juice, the colony counts of all <em>Penicillium</em> spp. were below the quantification limit (1.00 log CFU/mL) at the PL total fluence of 81.00 J/cm². The Weibull model was most suitable for kinetic fitting of the inactivation process of patulin-producing fungi by PL treatment. Besides, the results showed PL treatment caused significant deformation and rupture of fungal spores, leading to the release of cell contents and ultimately inactivating <em>Penicillium</em> spp. through combined photophysical and photochemical effects. The PL treatment effectively inactivates <em>Penicillium</em> spp. in apple juice, providing an efficient method for bacterial control in fruit juices.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"175 ","pages":"Article 111343"},"PeriodicalIF":5.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel coating formulated with sodium alginate loaded with a mixed lactic acid bacteria culture to control anthracnose in guava and mango","authors":"Karina Felix Dias Fernandes ,&nbsp;Kataryne Árabe Rimá de Oliveira ,&nbsp;Ítallo Fernandes Cirilo ,&nbsp;Maiara da Costa Lima ,&nbsp;Marcos Paz Saraiva Câmara ,&nbsp;Evandro Leite de Souza","doi":"10.1016/j.foodcont.2025.111341","DOIUrl":"10.1016/j.foodcont.2025.111341","url":null,"abstract":"<div><div>Guava and mango are tropical fruits widely consumed due to their pleasant flavor and nutritional composition. Anthracnose caused by <em>Colletotrichum</em> spp. Is considered a primary post-harvest disease affecting these fruits. Applying biocontrol agents, such as lactic acid bacteria (LAB), combined with coating materials is an innovative fruit biopreservation method. This study investigated the effects of a novel sodium alginate coating loaded with a mixed culture of LAB in controlling anthracnose caused by different pathogenic <em>Colletotrichum</em> isolates in guava (cv.) Paluma and mango (cv.) Palmer during 15 days of room temperature storage (25 ± 0.5 °C). A mixed culture formed by three different LAB species strains (<em>Levilactobacillus brevis</em> 59, <em>Lactiplantibacillus pentosus</em> 129, and <em>Limosilactobacillus fermentum</em> 263) was tested against ten target <em>Colletotrichum</em> isolates. The mixed culture inhibited the mycelial growth of all target <em>Colletotrichum</em> isolates <em>in vitro</em>, with up to 100 % inhibition. In the nutrient competition method, the mixed LAB culture survived and caused mycelial growth inhibition even at low nutrient concentrations. The coatings showed sizes of LAB cell subpopulations with non-permeabilized membrane (viable cells, PI-CFDA+) of &gt;31 % at the end of refrigerated storage and viable cell counts of &gt;7 log CFU/g. Applying coatings with sodium alginate loaded with mixed LAB culture delayed the development and decreased the severity of anthracnose lesions in guava and mango artificially contaminated with either of the tested <em>Colletotrichum</em> isolates. The coating formulated with sodium alginate and mixed LAB culture can be effective for postharvest anthracnose biocontrol in guava and mango.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"175 ","pages":"Article 111341"},"PeriodicalIF":5.6,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}