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A LAMP-based colorimetric and fluorescence dual-channel assay for on-site identification of adulterated meat by a portable device 一种基于 LAMP 的比色和荧光双通道检测法,用于通过便携式设备现场识别掺假肉类
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-29 DOI: 10.1016/j.foodcont.2024.110984
Xun Fang , Wenyu Zhang , Huihui Su, Wenting Xie, Li Jia
{"title":"A LAMP-based colorimetric and fluorescence dual-channel assay for on-site identification of adulterated meat by a portable device","authors":"Xun Fang ,&nbsp;Wenyu Zhang ,&nbsp;Huihui Su,&nbsp;Wenting Xie,&nbsp;Li Jia","doi":"10.1016/j.foodcont.2024.110984","DOIUrl":"10.1016/j.foodcont.2024.110984","url":null,"abstract":"<div><div>Rapid on-site identification of meat adulteration is essential for ensuring food safety. The Loop-mediated isothermal amplification (LAMP) technique is an ideal solution for on-site diagnosis due to its simplicity, speed, high specificity, and sensitivity. In this study, we designed pork-specific primers for LAMP and optimized the LAMP reaction conditions. By incorporating hydroxynaphthol blue and calcein indicators into the reaction system, the LAMP results can be visually observed without the need for specialized equipment. We also engineered a self-designed, portable device that integrates LAMP colorimetric and fluorescence detection, termed LAMP-PD, to achieve both amplification and dual-channel detection. This device is cost-effective, priced at only $54.65. The detection results from LAMP-PD were found to be comparable to those of commercial spectrophotometers, confirming its accuracy. The LAMP-PD is capable of detecting pork at concentrations as low as 0.1% in adulterated meat samples, highlighting the high sensitivity of the LAMP assay. The portability and affordability of the LAMP-PD underscore its significant potential for on-site identification of adulterated meat samples.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"169 ","pages":"Article 110984"},"PeriodicalIF":5.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of chlorine and peroxyacetic acid efficacy in controlling Listeria innocua in a pilot-scale apple dump tank system 评估氯和过氧乙酸在试验规模苹果倾倒槽系统中控制无核李斯特菌的功效
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-29 DOI: 10.1016/j.foodcont.2024.110985
Yuan Su, Mengqian Hang, Xiaoye Shen, Jeanene M. Deavila, Mei-Jun Zhu
{"title":"Evaluation of chlorine and peroxyacetic acid efficacy in controlling Listeria innocua in a pilot-scale apple dump tank system","authors":"Yuan Su,&nbsp;Mengqian Hang,&nbsp;Xiaoye Shen,&nbsp;Jeanene M. Deavila,&nbsp;Mei-Jun Zhu","doi":"10.1016/j.foodcont.2024.110985","DOIUrl":"10.1016/j.foodcont.2024.110985","url":null,"abstract":"<div><div>The dump tank and flume system, treated with antimicrobial sanitizers, is the first and most critical step to control the cross-contamination of foodborne pathogens on apples in commercial packing lines. Previous studies on the efficacy of chlorine and peroxyacetic acid (PAA) during apple washing have been primarily conducted in laboratory-scaled settings, which may not accurately reflect the efficacy of commercial dump tank operation due to variations in processing parameters. This study employed a pilot-scale dump tank system containing 102 L of pilot-simulated dump tank water (pSDTW) to evaluate the efficacy of chlorine and PAA in reducing <em>Listeria innocua</em> on apples and its cross-contamination between apples and water. Chlorine and PAA significantly reduced the transfer of <em>L. innocua</em> to uninoculated apples and water, with greater sanitizer concentrations and extended processing times enhancing their efficacy. Specifically, chlorine at 25–100 ppm free chlorine (FC) for 0.5–10 min resulted in the reductions of <em>L. innocua</em> by 0.4–1.1 log<sub>10</sub> CFU/apple on inoculated apples and 3.5–5.4 log<sub>10</sub> CFU/ml in inoculated pSDTW in the presence of 1000 ppm chemical oxygen demand (COD), leading to a transfer of 0.4–3.5 log<sub>10</sub> CFU/apple to uninoculated apples. For PAA treatment, 20–80 ppm achieved reductions of 0.5–1.5 log<sub>10</sub> CFU/apple on inoculated apples and 0.7–4.3 log<sub>10</sub> CFU/ml in inoculated pSDTW, resulting in transfers of 3.5–4.2 log<sub>10</sub> CFU/apple to uninoculated apples. Elevating the organic load in pSDTW from 1000 to 4000 ppm COD significantly reduced the effectiveness of chlorine in reducing <em>L. innocua</em> and its cross-contamination, and the anti-<em>Listeria</em> efficacy of PAA in water. This study provides valuable insights into optimizing dump tank operations for commercial apple packing lines.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"169 ","pages":"Article 110985"},"PeriodicalIF":5.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomics based on GC-MS combined with chemometrics for geographical discrimination of garlic (Allium sativum L.) 基于气相色谱-质谱联用化学计量学的代谢组学在大蒜(Allium sativum L.)地理鉴别中的应用
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-29 DOI: 10.1016/j.foodcont.2024.110976
Victor dos S.A. Leite , Brena R.M. Ikehara , Natália R. Almeida , Geraldo H. Silva , Willian R. Macedo , Frederico G. Pinto
{"title":"Metabolomics based on GC-MS combined with chemometrics for geographical discrimination of garlic (Allium sativum L.)","authors":"Victor dos S.A. Leite ,&nbsp;Brena R.M. Ikehara ,&nbsp;Natália R. Almeida ,&nbsp;Geraldo H. Silva ,&nbsp;Willian R. Macedo ,&nbsp;Frederico G. Pinto","doi":"10.1016/j.foodcont.2024.110976","DOIUrl":"10.1016/j.foodcont.2024.110976","url":null,"abstract":"<div><div>Ensuring the geographic origin of products helps protect consumers against fraud and mislabeling. This study investigates the metabolomic profiles of garlic (<em>Allium sativum</em> L.) samples from Brazil and China using gas chromatography-mass spectrometry (GC-MS). Multivariate analysis Partial Least Squares Discriminant Analysis (PLS-DA) and Orthogonal PLS-DA (Ortho PLS-DA) revealed clear metabolic distinctions between the two sample groups with high classification accuracy. Brazilian garlic samples showed higher levels of sulfur compounds, such as allyl propyl sulfide, cysteine sulfonic acid, and S-carboxymethyl-L-cysteine, which contribute to its pungency and potential health benefits. Amino acids L-arginine, L-glutamic acid, and L-cysteine were also significantly increased in garlic from Brazil. In contrast, Chinese garlic exhibited higher intensities of sugars (e.g., D-fructose, D-mannose) and essential amino acids (L-tryptophan, L-valine, and L-leucine). These differences in metabolism are the result of distinct metabolic profiles. GC-MS-based untargeted metabolomic and chemometric analysis were able to differentiate the geographical origin of Brazilian and Chinese garlics and remain valuable to food authentication and quality control. Further studies are needed to confirm the biomarkers for origin verification here stated.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"169 ","pages":"Article 110976"},"PeriodicalIF":5.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a digital Net-Map tool to analyse multi-stakeholder networks in risk analysis of emerging food safety issues 开发数字网络地图工具,分析新食品安全问题风险分析中的多方利益相关者网络
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-29 DOI: 10.1016/j.foodcont.2024.110975
Niels van der Linden , Celine Meerpoel , Hanna Schebesta , Pieternel Luning
{"title":"Development of a digital Net-Map tool to analyse multi-stakeholder networks in risk analysis of emerging food safety issues","authors":"Niels van der Linden ,&nbsp;Celine Meerpoel ,&nbsp;Hanna Schebesta ,&nbsp;Pieternel Luning","doi":"10.1016/j.foodcont.2024.110975","DOIUrl":"10.1016/j.foodcont.2024.110975","url":null,"abstract":"<div><div>The circular economy brings sustainability benefits but also causes potential food safety issues as recycling can introduce new contaminants to food contact materials. These circular food safety issues cause changes in the risk analysis network. So far, social network analysis studies relevant to food safety investigated specific parts of the risk analysis network (such as risk assessment) and its formal stakeholders such as Food Safety Authorities. However, the risk analysis network also consists informal stakeholders, each with their own knowledge and views. A comprehensive analysis of risk analysis networks addressing circularity-related food safety issues from a multi-stakeholder perspective is yet lacking. This study aimed to explore the complex risk analysis network for paper recycling in Belgium. An adjusted and digitalised network mapping methodology, Net-Mapping, was developed to identify the stakeholders, to assess their goals and influence, to determine the different linkages types between them, and to elicit constraints. Forty-one identified stakeholders from science, policy, and society were interconnected through four linkage types (‘legally required information sharing’, ‘voluntary information sharing’, ‘data generation request’, and ‘public communication’). Results show federal policy stakeholders are central in all networks, whereas science and society stakeholders gain influence in the informal networks. Barriers hindering collaboration in the networks are a lack of data and challenges in information exchange. The Net-Mapping insights can assist scientists in gathering risk assessment data, guide policymakers in targeting interventions, and raise stakeholder awareness of collaborations. Future research could compare risk analysis networks across countries for the same food safety issue, or examine the risk analysis networks for different food safety issues in the same country.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"169 ","pages":"Article 110975"},"PeriodicalIF":5.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A rapid and nondestructive quantitative detection method for total and organic selenium in selenium-enriched kefir grain based on hyperspectral imaging 基于高光谱成像的富硒酸乳酒糟中总硒和有机硒的快速无损定量检测方法
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-26 DOI: 10.1016/j.foodcont.2024.110958
Menghui Li , Jinyi Yang , Xing Guo , Rui Sun , Gechao Zhang , Wenhui Liu , Yuanye Liu , Zhouli Wang , Yahong Yuan , Tianli Yue
{"title":"A rapid and nondestructive quantitative detection method for total and organic selenium in selenium-enriched kefir grain based on hyperspectral imaging","authors":"Menghui Li ,&nbsp;Jinyi Yang ,&nbsp;Xing Guo ,&nbsp;Rui Sun ,&nbsp;Gechao Zhang ,&nbsp;Wenhui Liu ,&nbsp;Yuanye Liu ,&nbsp;Zhouli Wang ,&nbsp;Yahong Yuan ,&nbsp;Tianli Yue","doi":"10.1016/j.foodcont.2024.110958","DOIUrl":"10.1016/j.foodcont.2024.110958","url":null,"abstract":"<div><div>Rapid monitoring of total and organic selenium content of Kefir grain is important for the development of selenium-enriched foods. First, a portable hyperspectral system was used to obtain spectra of selenium-enriched Kefir grain. Second, the characteristic variables were screened based on competitive adaptive reweighted sampling (CARS), bootstrapping soft shrinkage, genetic algorithm, and particle swarm optimization. Then, the quantitative prediction performance of extremely randomized trees, partial least squares (PLS), and least squares support vector machine models were compared. For the prediction of total selenium content, CARS-PLS performed the best, with a prediction set correlation coefficient and relative prediction deviation (RPD) value of 0.97 and 3.88, respectively. For organic selenium, the CARS-PLS predicted set correlation coefficients and RPD values were 0.94 and 2.97, respectively. CARS-PLS realized the rapid prediction of total and organic selenium content within 1 min. This provides new ideas for screening selenium-enriched microorganisms and rapid detection of selenium content.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"169 ","pages":"Article 110958"},"PeriodicalIF":5.6,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of tropane alkaloid contaminations in unprocessed soybeans and their fate in food and feed processing 检测未加工大豆中的托烷生物碱污染及其在食品和饲料加工中的去向
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-21 DOI: 10.1016/j.foodcont.2024.110963
Bernhard Blank-Landeshammer , Christa Ranetbauer , Julian Weghuber
{"title":"Detection of tropane alkaloid contaminations in unprocessed soybeans and their fate in food and feed processing","authors":"Bernhard Blank-Landeshammer ,&nbsp;Christa Ranetbauer ,&nbsp;Julian Weghuber","doi":"10.1016/j.foodcont.2024.110963","DOIUrl":"10.1016/j.foodcont.2024.110963","url":null,"abstract":"<div><div><em>Datura stramonium</em> and other nightshade plants commonly occur as weeds and have the potential to contaminate crops with toxic tropane alkaloids. Here, the contamination of soybeans with the tropane alkaloids atropine and scopolamine after co-harvesting with thornapple plants is reported. The potential route of contamination was found to be independent of the thornapple seeds. The further fate of tropane alkaloids in downstream processes was evaluated in laboratory-scale tofu and soybean oil production. It was determined that a relatively small portion of the alkaloids is persistent in the tofu, while the reduction in soybean oil and press cake is less pronounced. No regulation is in place for the maximum amount of tropane alkaloids in soybeans, but the limits for other food crops were exceeded both in the raw and processed goods.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":"Article 110963"},"PeriodicalIF":5.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-class, multi-residue analysis of pesticide residues in fishery products using LC and GC-MS/MS 利用 LC 和 GC-MS/MS 对水产品中的农药残留进行多类别、多残留分析
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-21 DOI: 10.1016/j.foodcont.2024.110959
Dahae Park , Ji Young Kim, Jang-Duck Choi, Guiim Moon, Dong Woo Shin
{"title":"Multi-class, multi-residue analysis of pesticide residues in fishery products using LC and GC-MS/MS","authors":"Dahae Park ,&nbsp;Ji Young Kim,&nbsp;Jang-Duck Choi,&nbsp;Guiim Moon,&nbsp;Dong Woo Shin","doi":"10.1016/j.foodcont.2024.110959","DOIUrl":"10.1016/j.foodcont.2024.110959","url":null,"abstract":"<div><div>Pesticide residues in agricultural products and the environment have been studied extensively over the years. Through the excessive or illegal use of pesticides, residues enter the surrounding environment and are absorbed by animals. Livestock and fishery products are subject to pesticide residue regulation, which includes the establishment of maximum residue limits. However, currently, fishery products are regulated only for ethoxyquin, neglecting the potential for bioaccumulation. This study aims to develop a method for the multi-class, multi-residue analysis of pesticide residues in fishery products—flatfish, eel, shrimp, and Manila clam—based on the QuEChERS method. A total of 327 compounds, including 263 pesticides and their metabolites, were identified using liquid chromatography and gas chromatography–tandem mass spectrometry. The method can quantify 263 pesticide residues in flatfish, 265 in eel, 264 in shrimp, and 263 in Manila clam, respectively. Additionally, the method can be applied to quantify 244 pesticide residues in seaweed. The method proves effective and useful for monitoring both food safety and environmental contamination.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":"Article 110959"},"PeriodicalIF":5.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Critical factors associated with Maillard Reaction Products in different meats: an integrative review 与不同肉类中 Maillard 反应产物相关的关键因素:综合评述
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-21 DOI: 10.1016/j.foodcont.2024.110960
Larissa de Oliveira Santos , Anna Liszzarda Silva Ribeiro , Keithy Michelle de Oliveira Lima , Isamira Reis Portela dos Santos , Beatriz Santos de Almeida , Márcia Filgueiras Rebelo de Matos , Fernanda Doring Krumreich , Lílian Lessa Andrade , Walison Fabio Ruggiero , Laise Cedraz Pinto Matos
{"title":"Critical factors associated with Maillard Reaction Products in different meats: an integrative review","authors":"Larissa de Oliveira Santos ,&nbsp;Anna Liszzarda Silva Ribeiro ,&nbsp;Keithy Michelle de Oliveira Lima ,&nbsp;Isamira Reis Portela dos Santos ,&nbsp;Beatriz Santos de Almeida ,&nbsp;Márcia Filgueiras Rebelo de Matos ,&nbsp;Fernanda Doring Krumreich ,&nbsp;Lílian Lessa Andrade ,&nbsp;Walison Fabio Ruggiero ,&nbsp;Laise Cedraz Pinto Matos","doi":"10.1016/j.foodcont.2024.110960","DOIUrl":"10.1016/j.foodcont.2024.110960","url":null,"abstract":"<div><div>The global consumption of meat such as chicken, pork, or fish is still more representative than beef. The thermal processing applied to these meats contributes to Maillard reaction and its products (MRP), which, despite their sensorial importance, are implicated in toxic effects on human health. This study aimed to review data on preparation conditions and the main factors that influence the formation of MRP in different meat products. High MRP levels were found in meat prepared by frying for acrylamide (AA), carboxyethyllysine (CEL), carboxymethyllysine (CML), hydroxymethylfurfural (HMF), and furans; sterilized/UHT for CEL and CML; in baked products for HMF. Regarding the type of meat, the highest levels of CML, CEL, and HMF were from fish, and the highest level of AA was from pork. Longer and higher temperatures, such as frying, grilling, and roasting, were associated with higher MRP levels. Sorbitol as a cryoprotectant, breading techniques with antioxidants such as plant extracts (grape), and mixed flours with the addition of chitosan, gum arabic, quinoa, or chia contributed to MRP mitigation. Fish, grilled, and roasted products had high levels of MRP despite being recommended in healthy diets. Critical factors in cooking meat and its products should be included in dietary guidelines and defined as good practice criteria in food production systems.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":"Article 110960"},"PeriodicalIF":5.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The inactivation of Shiga toxin-producing Escherichia coli (STEC) and Listeria monocytogenes using isochoric freezing in raw milk and carrot juice 在生牛奶和胡萝卜汁中使用等温冷冻法灭活产志贺毒素大肠杆菌(STEC)和单核细胞增生李斯特菌
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-19 DOI: 10.1016/j.foodcont.2024.110957
Alison Lacombe , Beatrice L. Harvey , Jared Van Blair , Natalie Chapman , Cristina Bilbao-Sainz , Tara McHugh , Boris Rubinsky , Vivian C.H. Wu
{"title":"The inactivation of Shiga toxin-producing Escherichia coli (STEC) and Listeria monocytogenes using isochoric freezing in raw milk and carrot juice","authors":"Alison Lacombe ,&nbsp;Beatrice L. Harvey ,&nbsp;Jared Van Blair ,&nbsp;Natalie Chapman ,&nbsp;Cristina Bilbao-Sainz ,&nbsp;Tara McHugh ,&nbsp;Boris Rubinsky ,&nbsp;Vivian C.H. Wu","doi":"10.1016/j.foodcont.2024.110957","DOIUrl":"10.1016/j.foodcont.2024.110957","url":null,"abstract":"<div><div>Isochoric freezing is a novel food preservation method that may maintain the quality and safety of products by simultaneously applying low temperatures and high pressures. This work aims to determine whether isochoric freezing can improve food safety by inactivating Shiga toxin-producing <em>Escherichia coli</em> (STEC) and <em>Listeria monocytogenes</em> in raw milk and carrot juices. Raw milk and carrot juice samples were inoculated with cocktails of either STEC or <em>L</em>. <em>monocytogenes</em> and then subjected to isochoric freezing conditions in pressurized chambers set at −5, −10, and −15 °C and treated for 1, 5, 7, or 10 days. Following treatment, the product was sampled for surviving pathogens. The results demonstrated that a 5-log reduction of both STEC and <em>L. monocytogenes</em> can be achieved for raw milk and carrot juice without adversely affecting quality. For STEC inoculated in raw milk, both the linear and Weibull models suggest that 10 days is required to achieve 5-log reduction at −10 °C. Decreasing the temperature to −15 °C led to accelerated log reduction but induced phase separation in the milk. <em>Listeria</em> in raw milk demonstrated a biphasic reduction, indicating 1.3 days is required for a 5-log reduction at −10 °C. In comparison, pathogens demonstrated faster log reduction in carrot juice due to its intrinsic properties. The survival curves for STEC-inoculated carrot juice demonstrated that the 5-log reduction times were 6.9 days at −10 °C and 3.8 days at −15 °C. <em>L. monocytogenes</em> in carrot juice required 1.5 days at −10 °C for a 5-log reduction and was eliminated (7 log cfu/ml) within 24 h at −15 °C. Isochoric freezing could be an option for milk and carrot juice processors wishing to improve food safety without applying heat. This method is not a one-size-fits-all solution, and the time and temperature will need further optimization depending on the target pathogen and intended commodity.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":"Article 110957"},"PeriodicalIF":5.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive detection of Vibrio parahaemolyticus via a dual-recognition colorimetric biosensor comprising Cefe-PEG-MNPs and apt-Fe@PDA 通过由头孢-PEG-MNPs 和 apt-Fe@PDA 组成的双识别比色生物传感器灵敏检测副溶血性弧菌
IF 5.6 1区 农林科学
Food Control Pub Date : 2024-10-19 DOI: 10.1016/j.foodcont.2024.110955
Hongyong Lin , Tengyang Liao , Yaoyao Sang , Jin Mao , Yu Liu , Bin Zhang , Di Sun , Wei Jiang
{"title":"Sensitive detection of Vibrio parahaemolyticus via a dual-recognition colorimetric biosensor comprising Cefe-PEG-MNPs and apt-Fe@PDA","authors":"Hongyong Lin ,&nbsp;Tengyang Liao ,&nbsp;Yaoyao Sang ,&nbsp;Jin Mao ,&nbsp;Yu Liu ,&nbsp;Bin Zhang ,&nbsp;Di Sun ,&nbsp;Wei Jiang","doi":"10.1016/j.foodcont.2024.110955","DOIUrl":"10.1016/j.foodcont.2024.110955","url":null,"abstract":"<div><div>Highly accurate and sensitive detection of <em>Vibrio parahaemolyticus</em> is crucial for ensuring seafood safety and preventing the transmission of foodborne diseases. Herein, a dual-recognition strategy-driven colorimetric biosensor for the detection of <em>V. parahaemolyticus</em> was developed. This biosensor was based on the use of PEG-mediated cefepime-functionalized magnetic nanoparticles (Cefe-PEG-MNPs) and aptamer-modified Fe-doped polydopamine (Fe@PDA) nanozymes. The magnetic platform Cefe-PEG-MNPs was introduced to enhance the enrichment efficiency of <em>V. parahaemolyticus</em>, achieving a capture efficiency exceeding 90%. The Fe@PDA nanozyme exhibited notable peroxidase mimetic enzyme activity. The colorimetric biosensor developed for <em>V. parahaemolyticus</em> detection demonstrated good sensitivity, with a detection range spanning from 2.1 × 10<sup>1</sup> to 2.1 × 10<sup>6</sup> CFU/mL, and a limit of detection of 21 CFU/mL. Moreover, the biosensor demonstrated remarkable specificity, achieving good recovery rates from 95.80% to 103.80% in <em>Penaeus vannamei</em> with a relative standard deviation of less than 1.38%. The newly developed colorimetric biosensor offers a remarkable opportunity for the accurate and sensitive detection of <em>V</em>. <em>parahaemolyticus</em> in seafood.</div></div>","PeriodicalId":319,"journal":{"name":"Food Control","volume":"168 ","pages":"Article 110955"},"PeriodicalIF":5.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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