Jurnal Teknologi Laboratorium最新文献_第2页

Effect of Fetal Bovine Serum Concentration towards Vero Cells Growth on Culture in DMEM Medium 胎牛血清浓度对DMEM培养基中Vero细胞生长的影响

Jurnal Teknologi Laboratorium Pub Date : 2022-12-30 DOI: 10.29238/teknolabjournal.v11i2.313

Bella Niken Ikasari, Salman Alfarizi, S. Fauziyah, Puspa Wardhani, Aryati, S. Soegijanto, T. H. Sucipto

{"title":"Effect of Fetal Bovine Serum Concentration towards Vero Cells Growth on Culture in DMEM Medium","authors":"Bella Niken Ikasari, Salman Alfarizi, S. Fauziyah, Puspa Wardhani, Aryati, S. Soegijanto, T. H. Sucipto","doi":"10.29238/teknolabjournal.v11i2.313","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.313","url":null,"abstract":"Cell culture requires a suitable medium and environment for in vivo conditions. Conditions are created by regulating temperature, pH, oxygen, CO2, osmotic pressure, surfaces to adhere to cells, nutrients, and vitamins, protection against toxic substances, hormones, and growth factors that regulate cell growth and differentiation. In general, good cell growth occurs at a pH of 7.0 - 7.6. Fetal Bovine Serum (FBS) is one of the supplement media to support cell growth. The purpose of this study was to determine the effect of using FBS supplementation with various concentrations on vero cell culture media on DMEM (Dulbecco's modified eagle medium) media. Vero cell culture on DMEM media has been successfully carried out using FBS by observing its growth for 14 days. Vero cell culture process begins with the manufacture of media consisting of DMEM and FBS. The cell density of each well at 5% and 10% concentration media was 1017 cells/well. By varying the concentration of FBS in the culture medium, the difference in the number of cells produced can be observed under the microscope. The results showed that the number of Vero cells produced from DMEM media with 10% FBS concentration was higher than 5% FBS concentration. Thus, this study proves that the higher the concentration of FBS in the medium, the more it supports the cell growth process in the Vero cell culture process.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43926121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The wound healing effect of Morinda citrifolia leaf extract and biomolecular analysis on inflammation and proliferation stages in Wistar rats. 森达叶提取物对Wistar大鼠创伤愈合的影响及对炎症和增殖阶段的生物分子分析。

Jurnal Teknologi Laboratorium Pub Date : 2022-12-30 DOI: 10.29238/teknolabjournal.v11i2.369

Grace Noviyanthi Sinambela, Erny Tandanu, Refi Ikhtiari

{"title":"The wound healing effect of Morinda citrifolia leaf extract and biomolecular analysis on inflammation and proliferation stages in Wistar rats.","authors":"Grace Noviyanthi Sinambela, Erny Tandanu, Refi Ikhtiari","doi":"10.29238/teknolabjournal.v11i2.369","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.369","url":null,"abstract":"This research aims to evaluate the wound healing effect of noni’s leaf extract (Morinda citrifolia L.) based on phytochemical analysis, the number of fibroblast cells, wound diameter, healing time, TNF-a and IL-1 at inflammation stage, PDGF and TGF-b at proliferation stage. This study is a true experimental with a post-test-only control group design. We divided 25 male Wistar rats into five groups; positive control (with povidone-iodine), negative control (with no treatment), treated by NLEE 20%, 25%, and 30%. NLEE has good physical characteristics based on the value of ethanol soluble extract, water-soluble extract, water content, ash content, and acid insoluble ash content. GCMS analysis showed bioactive compounds such as N-ethyl hydrazine carbothioamide (31.57%), 2-furancarboxaldehyde 5-hydroxymethyl (15,64%), 2E-3,7,11,15-tetramethyl-2-hexadecene-1-of (7.08%), 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one (4.29%), D:C-friedooleana-7,9(11)-dien-3-ol,3beta (4.04%), while the rest are considered as the decanoic acids methyl ester, piperazine (3%) and vitamin E (1%). We observed the slight effects of NLEE 20%, 25%, and 30% on the number of fibroblasts, wound diameter, healing time, TNF-a, IL-1, PDGF, and TGF-b. The optimum concentration of NLEE at any treatment was 20%. However, there were no significant differences between groups based on a two-way ANOVA analysis.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47908878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of natural antioxidant compound in Cymbopogon nardus as anti-cancer drug via HSP-70 inhibitor 水杨中天然抗氧化化合物通过HSP70抑制剂作为抗癌药物的潜力

Jurnal Teknologi Laboratorium Pub Date : 2022-12-14 DOI: 10.29238/teknolabjournal.v11i2.372

Rofiatun Solekha, Putri Ayu Ika Setiyowati, Eka Febrianti Wulandari, Lilis Maghfuroh

{"title":"Potential of natural antioxidant compound in Cymbopogon nardus as anti-cancer drug via HSP-70 inhibitor","authors":"Rofiatun Solekha, Putri Ayu Ika Setiyowati, Eka Febrianti Wulandari, Lilis Maghfuroh","doi":"10.29238/teknolabjournal.v11i2.372","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.372","url":null,"abstract":"Citronella grass (Cymbopogon nardus) is a plant containing many metabolite compounds which prevent and treat various diseases, one of which is cancer. Antioxidant compounds found in citronella have been shown to improve the immune system by increasing cytokines. The activity of changing homeostasis generates free radicals. Free radicals causing protein damage so that Heat Shock Protein-70 (HSP70) is overexpressed. HSP70 has a role as a chaperon. Mutations in the anti-apoptotic protein HSP70 are one of the causes of cancer. This current research aims to determine the potential of compounds present in the citronella plant stem as anti-cancer through inhibition of HSP-70. The method was a bioinformatics approach, namely the in-silico method which provided a simulation of binding protein ligands to HSP-70 as inhibitor mechanism. The results of this study indicated that there was a potential for citronella compounds, namely spathulenol binding to HSP-70. Spathulenol compounds interact with Hsp70 via the positions Thr204, Gly12, Gly203, Thr14, Lys71, Asp10, Val369, Asp199, Val337, Gly338, Asp366, Gly339, Pro365, Glys201, & Glys202 with Van der Waals bonds and hydrogen bonds on Thr13. In the complex, there was one unfavorable bond formed on the O atom of the query ligand. From the results above, it can be concluded that the Spathulenol compound is predicted to act as an inhibitor of Hsp70 protein activity because it inhibits the binding site of the native ligand on Hsp70. The stability of the binding interaction produced by Spathulenol allows a response to Hsp70 inhibitor activity. By inhibiting the activity of Hsp70 inhibitors, it is possible to inhibit the formation and proliferation of cancer cells","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47432283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Pathogenic Bacteria in Blood Cockle (Anadara granosa) using 16S rRNA Gene 用16S rRNA基因鉴定血蛤致病菌

Jurnal Teknologi Laboratorium Pub Date : 2022-12-14 DOI: 10.29238/teknolabjournal.v11i2.364

Meutia Srikandi Fitria, A. Kartika, A. Mukaromah, Nurfi Ismatul Unasiah

{"title":"Identification of Pathogenic Bacteria in Blood Cockle (Anadara granosa) using 16S rRNA Gene","authors":"Meutia Srikandi Fitria, A. Kartika, A. Mukaromah, Nurfi Ismatul Unasiah","doi":"10.29238/teknolabjournal.v11i2.364","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.364","url":null,"abstract":"Blood cockle (Anadara granosa) is one of the marine resources in Indonesia that contains protein. Processing of blood cockles that are not perfect or raw will be contaminated with pathogenic bacteria that live in the waters. Pathogenic bacteria cause foodborne disease, which is a disease in humans caused by food. Several bacterial pathogens that cause foodborne disease are Escherichia sp. Pseudomonas sp., and Vibrio sp. Pathogenic bacteria in blood cockle should be identified using 16S rRNA as molecular identification. Samples were isolated using BAP, HIA, and BHI media. Bacteria from BHI media were isolated. Isolation DNA was isolated using the phenol-CIAA method. The DNA isolates were amplified by the PCR method based on the 16S rRNA target gene, then visualized the DNA with 2% agarose gel electrophoresis and sequencing. Bacterial colonies produced from BAP media for isolates BVA1, BVA9, and BVA10 were ß-hemolysis. Visualization of hemolytic bacterial DNA in blood cockle culture amplified about 1500 bp. Whereas the results of the sequencing analyzed by BLAST on the NCBI database and the Mega X program for BVA1 and BVA10 isolates showed similarity to Vibrio sp. bacteria, whereas BVA9 isolates showed similarity to Bacterium whose species still unknown. The conclusion showed that blood cockle had close simililarity with with Vibrio sp. and Bacterium.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42824297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and quick isolation method to obtain a huge number of fibroblasts like mesenchymal stem cells from human umbilical cord 一种从人脐带中获得大量成纤维细胞样间充质干细胞的简单快速分离方法

Jurnal Teknologi Laboratorium Pub Date : 2022-11-28 DOI: 10.29238/teknolabjournal.v11i2.385

Sudhir Bhatia

{"title":"A simple and quick isolation method to obtain a huge number of fibroblasts like mesenchymal stem cells from human umbilical cord","authors":"Sudhir Bhatia","doi":"10.29238/teknolabjournal.v11i2.385","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.385","url":null,"abstract":"In this publication, we have developed a simple and quick isolation method of stem cells from the umbilical cord. Usually, the umbilical cord is a waste product during the child delivery procedure, but the umbilical cords contain valuable stem cells called mesenchymal stroma stem cells (also called MSCs), which can be the basis for different applications e.g., testing the effect of therapeutic compounds as well as the development of therapeutic applications for the cure of degenerative diseases like bone and cartilage related. These stem cells can be used to create new artificial organs too. The umbilical cord was cut with sterile scissors into small pieces (1-2 mm size) and these pieces were placed on 6 well culture plates. The cell culture media was added to these plates after 5-10 minutes placing these plates in a CO2 incubator. The successful growth of cells was observed after 7 days in wells, where pieces of umbilical cord were implanted. Microscopic observations show how the cells are oozing out from these pieces. These cells were isolated and passaged further. These adhere to the plastic surface, were fibroblast shaped, and were isolated successfully in different experiments.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42959635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of temulawak (Curcuma xanthorrhiza) ointments as wound healing agents in wistar rats 黄姜黄软膏对wistar大鼠创面愈合的影响

Jurnal Teknologi Laboratorium Pub Date : 2022-11-14 DOI: 10.29238/teknolabjournal.v11i2.376

Jessica Panjaitan, E. Girsang, L. Chiuman

引用次数: 0

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis IR-Bagendit水稻叶片对大鼠肾小管变性和坏死的影响

Jurnal Teknologi Laboratorium Pub Date : 2022-10-31 DOI: 10.29238/teknolabjournal.v11i2.352

B. Santosa, Erick Erianto Arif, Ana Hidayati Mukaromah

{"title":"The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis","authors":"B. Santosa, Erick Erianto Arif, Ana Hidayati Mukaromah","doi":"10.29238/teknolabjournal.v11i2.352","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.352","url":null,"abstract":"Plumbum exposure still becomes a health problem in Indonesia. Plumbum (Pb) is widely used in various industries; thus, it can cause health problems, including impaired kidney functions. The accumulation of Pb rise to degeneration and necrosis of renal tubule cells. Therefore, preventive efforts are necessary to inhibit renal function deterioration. Infused extract of IR Bagendit rice leaf from Blora contains protein metallothionein which could bind PB covalently. This study aims to determine the supplementation of IR Bagendit rice leaf infusion to inhibit degeneration and necrosis of renal tubular cells exposed to Pb. The experimental research method was conducted using Wistar albino rats divided into five groups: negative control group, positive group, and treatment groups 1, 2, and 3. Each group consisted of six rats. The negative control group only received a placebo, and the positive group only received Pb acetate 60 mg/kg BW/day. Meanwhile, treatment groups 1, 2, and 3 received 0.2 ml, 0.4 ml, and 0.8 ml IR Bagendit rice leaf infusions successively and Pb acetate 60 mg/kg BW/day. The experiment was conducted for 60 days. Histological preparations of tubular cells were colored using hematoxylin-eosin. Normal cell differences, degeneration, and necrosis of each group were tested using Kruskal Wallis and Mann Whitney tests.This study has revealed that the average normal cells in the positive control group are lower than those in the negative control group and treatment groups 1, 2, and 3. This difference is insignificant with the p = 0.477. Moreover, treatment groups 1, 2, and 3 show decreased cell degeneration and necrosis with significant differences of p = 0.000 and 0.001. IR Bagendit rice leaf infusions could inhibit degeneration and necrosis of renal tubular cells exposed to Pb.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46933464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison between use courier transport with pneumatic tube system on the results of routine hematology test, Prothrombin Time (PPT), Activated Partial Thromboplastin Time (APTT), and potassium 使用信使运输和气动管道系统对常规血液学检查结果、凝血酶原时间（PPT）、活化部分凝血活酶时间（APTT）和钾的比较

Jurnal Teknologi Laboratorium Pub Date : 2022-10-31 DOI: 10.29238/teknolabjournal.v11i2.342

Yoki Setyaji, Kurnia Fitriasari, Tri Novitasari, Norma Agustin Palupi

{"title":"Comparison between use courier transport with pneumatic tube system on the results of routine hematology test, Prothrombin Time (PPT), Activated Partial Thromboplastin Time (APTT), and potassium","authors":"Yoki Setyaji, Kurnia Fitriasari, Tri Novitasari, Norma Agustin Palupi","doi":"10.29238/teknolabjournal.v11i2.342","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.342","url":null,"abstract":"Pneumatic tube system (PTS) is a transport medium that is widely used in hospitals. The samples transported via PTS would get vibrations due to changing air velocity and pressure. This unstable pressure could cause pre-analytic errors in laboratory measurements. It happened because it damages erythrocytes and lymphocytes and causes haemolysis. It has been demonstrated that these changes can alter the quality of samples and induce haemolysis by leading primarily to increase in lactate dehydrogenase (LDH) concentrations, and potassium concentration. Haemolysis is dined as the release of intracellular components of erythrocytes and other blood cells into the extracellular space of blood. It can also affect the results of in vitro platelet function test. Incorrect test results can affect the diagnosis and the treatment for patients Every hospital that uses PTS are advised to validate and investigate that PTS has the possibility of haemolysis and impacts laboratory results of blood specimens. The purpose of the study was to validate and know comparison between use courier transport with using PTS on the results of routine haematology tests, PPT, APTT, and potassium. This research was experimental research by using posttest without control. The research used statistical test which was paired t-test. The research was conducted at the Integrated Clinical Laboratory Installation of the Gadjah Mada University Academic Hospital in August – October 2020 with 30 preoperative patients. Data were analysed with Prism GraphPad 8 software. There was no significant difference between WBC, RBC, Hb, HCT, MCV, MCH, MCHC, PPT, and APTT. Moreover, there was a significant difference between the results of PLT and potassium in the samples transported via PTS and delivered by courier transport.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45876842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Identification of an Alkaloid Compound from Bebuas Leaves (Premna serratifolia) as an Anti-Inflammatory in White Rats (Rattus norvegicus) 褐家鼠(Rattus norvegicus)抗炎用山茱萸叶生物碱化合物的分离与鉴定

Jurnal Teknologi Laboratorium Pub Date : 2022-10-31 DOI: 10.29238/teknolabjournal.v11i2.275

Muhammad Irhash Shalihin, Muhaimin, M. Latief

{"title":"Isolation and Identification of an Alkaloid Compound from Bebuas Leaves (Premna serratifolia) as an Anti-Inflammatory in White Rats (Rattus norvegicus)","authors":"Muhammad Irhash Shalihin, Muhaimin, M. Latief","doi":"10.29238/teknolabjournal.v11i2.275","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.275","url":null,"abstract":"Bebuas (Premna serratifolia) traditionally is used by Malay people as a traditional medicine to restore health. This indicates it contains chemicals that potentially have an anti-inflammatory activity. The results of phytochemical screening showed that the ethanol extract contains phenolics, alkaloids, flavonoids, steroids and saponins. Meanwhile, hexane extract only contains steroids. Anti-inflammatory activity evaluation on crude extracts showed that the ethanol extract was more active than the hexane extract. Therefore, the isolation step was continued to ethanol extract with the targeted compound was an alkaloid. Extraction was conducted by using acid-base and partition techniques to give the alkaloids fraction (EaBW). EaBW is then further separated by liquid vacuum column technique using gradual polarity elution, with eluent of ethyl acetate (Ea) - methanol and obtained 5 fractions. The isolate was obtained from F.3. The isolate showed it had anti-inflammatory activity with ED50 = 5.45 mg/KgBW. The UV-Vis and FT-IR spectra patterns of the isolate have similarities with ciprofloxacin as well as its physical characteristics which are hygroscopic yellowish crystal, and fluorescence property. Therefore, the isolated compound is thought to have a similar structure to ciprofloxacin.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43469664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic effects of Kaempferia Galanga against Plasmodium berghei: in vivo study 山奈对伯氏疟原虫的预防作用：体内研究

Jurnal Teknologi Laboratorium Pub Date : 2022-06-30 DOI: 10.29238/teknolabjournal.v10i2.283

N. Lusiyana

{"title":"Prophylactic effects of Kaempferia Galanga against Plasmodium berghei: in vivo study","authors":"N. Lusiyana","doi":"10.29238/teknolabjournal.v10i2.283","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i2.283","url":null,"abstract":"Recently, medicinal plants have been the main source in treating malaria. Kaempferia galanga was investigated as an antioxidant, and anti-inflammation. In vivo study of K. galanga had been verified as antimalarial for severe malaria. The objective of this study is to investigate the chemoprophylactic effect, body composition, and blood parameters of K. galanga against Plasmodium berghei. The prophylactic effects were determined by employing K. galanga (50; 100 and 200 mg/kg) in mice infected with P. berghei. Mice were subdivided into 4 groups (control negative and 3 treatment groups). The treatment groups received K. galanga daily for 3 days before the inoculation of P. berghei. Each mice were inoculated with the infected blood containing 106 of P. berghei. Parasitemia and body weight were calculated every day until 5 days post-inoculation, and the blood parameters was monitored in day 5. The parasitemia on the fifth days after inoculation revealed a significant suppresses effect compared to control (p<0.05). The suppressed effect by doses 50 mg/kg; 100 mg/kg; 200 mg/kg and negative control were 0.83%; 1.96%; 2.82%, and 17.8% respectively. The body weight on treatment groups was normal, but the control group decreased (p<0.05). The blood parameters on treatment groups were normal compared to control group. The K. galanga possess the prophylactic effect, normal weight and blood parameters against Plasmodium berghei. ","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42246846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0