The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Abstract

Plumbum exposure still becomes a health problem in Indonesia. Plumbum (Pb) is widely used in various industries; thus, it can cause health problems, including impaired kidney functions. The accumulation of Pb rise to degeneration and necrosis of renal tubule cells. Therefore, preventive efforts are necessary to inhibit renal function deterioration. Infused extract of IR Bagendit rice leaf from Blora contains protein metallothionein which could bind PB covalently. This study aims to determine the supplementation of IR Bagendit rice leaf infusion to inhibit degeneration and necrosis of renal tubular cells exposed to Pb. The experimental research method was conducted using Wistar albino rats divided into five groups: negative control group, positive group, and treatment groups 1, 2, and 3.  Each group consisted of six rats. The negative control group only received a placebo, and the positive group only received Pb acetate 60 mg/kg BW/day. Meanwhile, treatment groups 1, 2, and 3 received 0.2 ml, 0.4 ml, and 0.8 ml IR Bagendit rice leaf infusions successively and Pb acetate 60 mg/kg BW/day. The experiment was conducted for 60 days. Histological preparations of tubular cells were colored using hematoxylin-eosin. Normal cell differences, degeneration, and necrosis of each group were tested using Kruskal Wallis and Mann Whitney tests.This study has revealed that the average normal cells in the positive control group are lower than those in the negative control group and treatment groups 1, 2, and 3. This difference is insignificant with the p = 0.477. Moreover, treatment groups 1, 2, and 3 show decreased cell degeneration and necrosis with significant differences of p = 0.000 and 0.001. IR Bagendit rice leaf infusions could inhibit degeneration and necrosis of renal tubular cells exposed to Pb.