Jurnal Teknologi Laboratorium最新文献_第8页

Pengembangan Prekultur Oxgall sebagai Sampel Klinis untuk Deteksi Salmonella typhi dengan Metode Real-time PCR 牛胆前培养物作为检测伤寒沙门氏菌临床样品的实时PCR方法

Jurnal Teknologi Laboratorium Pub Date : 2018-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V7I2.127

Annisa Pratiwi Gunawan, Ai Djuminar, E. Ernawati, Lidya Chaidir

{"title":"Pengembangan Prekultur Oxgall sebagai Sampel Klinis untuk Deteksi Salmonella typhi dengan Metode Real-time PCR","authors":"Annisa Pratiwi Gunawan, Ai Djuminar, E. Ernawati, Lidya Chaidir","doi":"10.29238/TEKNOLABJOURNAL.V7I2.127","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V7I2.127","url":null,"abstract":"Demam tifoid merupakan beban kesehatan masyarakat yang signifikan di negara berpenghasilan rendah yang disebabkan oleh Salmonella enterica serotype typhi (S.typhi). Manifestasi klinis demam tifoid bervariasi dan tidak spesifik, sehingga membuat diagnosis menjadi sulit. Dengan menggunakan oxgall untuk pra-inkubasi sebagai media kultur selektif sebelum amplifikasi Real-time PCR (RT-PCR) pada wholeblood menghasilkan diagnostic yang cepat dan sensitif. Tujuan dari penelitian ini adalah untuk mengetahui kinerja oxgall sebelum amplifikasi RT-PCR untuk deteksi Salmonella sp . Sebelum proses pengambilan sampel, dilakukan optimasi spike sampel untuk mengetahui bahwa reagen yang digunakan baik untuk spesimen klinis. Dalam proses sampel, sampel wholeblood diambil dari 30 pasien dengan positif Widal tes. Sampel darah vena dari pasien demam tifoid diambil pada hari diagnosis; 5 ml untuk kultur darah, dan 5 ml untuk RT-PCR. Bakteri ditanam di oxgall 10% (standar mikrobiologi laboratorium klinik) dan diinkubasi selama 6 jam (37 ° C) sebelum DNA bakteri diisolasi untuk deteksi RT-PCR. Hasil penelitian menunjukkan bahwa reagen RT-PCR baik digunakan untuk sampel klinis dan kultur darah lebih baik daripada RT-PCR dengan menggunakan oxgall (hasil kultur darah positif lebih dari 24 jam). Hal ini menunjukkan bahwa harus ada penelitian lebih lanjut mengenai durasi inkubasi dan konsentrasi oxgall pada RT-PCR dan pemilihan sampel klinis.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41405881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Aktivitas Antibakteri Kulit Buah Karika Dieng terhadap Shigella flexneri dan Escherichia coli 菌皮的抗菌活性用于石加拉跳蚤和Escherichia大肠杆菌

Jurnal Teknologi Laboratorium Pub Date : 2018-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V7I2.137

Dhiah Novalina, S. Sugiyarto, Ari Susilowati

引用次数: 1

Potensi Ekstrak Daun Carica Pubescens Sebagai Alternatif Antidiare Bakteri Vibrio cholerae dan Shigella dysentriae Pubescens收费单提取潜力作为替代抗霍乱弧菌和痢疾杆菌

Jurnal Teknologi Laboratorium Pub Date : 2018-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V7I2.138

T. Astuti, Wahid Syamsul Hadi

引用次数: 1

The Presence of Methanol in Alcoholic Beverages Analyzed Using Qualitative Method 用定性方法分析酒精饮料中甲醇的含量

Jurnal Teknologi Laboratorium Pub Date : 2018-09-05 DOI: 10.29238/TEKNOLABJOURNAL.V7I1.117

D. Navianti, Muhammad Ihsan Tarmizi, Sinta Nur Holifah

引用次数: 0

Antibiotic Resistant and Plasmid Conjugative Study of Salmonella typhi 伤寒沙门菌的耐药性及质粒结合研究

Jurnal Teknologi Laboratorium Pub Date : 2018-09-05 DOI: 10.29238/TEKNOLABJOURNAL.V7I2.134

Waleed Haji Saeed Akreyi, S. Yousif, M. Assafi

{"title":"Antibiotic Resistant and Plasmid Conjugative Study of Salmonella typhi","authors":"Waleed Haji Saeed Akreyi, S. Yousif, M. Assafi","doi":"10.29238/TEKNOLABJOURNAL.V7I2.134","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V7I2.134","url":null,"abstract":"The emergence of multi-drug resistant (MDR) bacteria has endangered the efficacy of antibiotics treatment of pathogenic bacteria worldwide. The aim of this research was to investigate the incidence of Salmonella enterica serovar Typhi in Duhok city, Iraq. Specimens of blood and stool were recruited from 267 patients. S. Typhi isolates were diagnosed depending on morphology, biochemical and serological tests. S. Typhi isolates were tested for their antibiotic resistance. Multi-drug resistant S. Typhi isolates were conjugated with E. coli HB101. The plasmid profile of transconjugants was investigated. 15/267 (5.6%) S. Typhi isolates were identified. Based on their biochemical tests, S. Typhi isolates were categorized into two biotypes (I, 26.66% and II, 73.33%). Four resistance patterns were observed. The resistant pattern to ampicillin and tetracycline was the higher (46.6%). Conjugation experiment showed that all antibiotic markers were transferred from S. Typhi to E. coli HB101 with a conjugation frequency of (0.38×10-5). 13.3% of the S. Typhi isolates were multi-drug-resistant resistant and had two small plasmids. Transconjugants E. coli acquired the resistance from the multi-drug resistant S. Typhi. Antibiotics treatment of the pathogens could be hindered by the constant rise of multi-drug-resistant. Further studies are needed to study the mobile genetic elements and their contribution to antibiotics resistance.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44079571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4