Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142773
M.R. Bhavana, Keshav Raghav Hegde, Malini Buvaneswaran, V.R. Sinija, Ashish Rawson, V. Hema
{"title":"The effect of co-precipitation and high-pressure treatment on functional and structural properties of millet and moringa protein","authors":"M.R. Bhavana, Keshav Raghav Hegde, Malini Buvaneswaran, V.R. Sinija, Ashish Rawson, V. Hema","doi":"10.1016/j.foodchem.2025.142773","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142773","url":null,"abstract":"Protein co-precipitation overcomes the limitations of individual proteins and improves their nutritional profile and functional properties. This study examined the impact of co-precipitation and high-pressure (HP) treatment on millet-moringa protein co-precipitate structure and functional properties. The co-precipitation has significantly (<em>p</em> < 0.05) improved the water holding (4.17 ± 0.02 g/g), oil holding (7.49 ± 0.16 g/g) capacity, and emulsifying stability index (60.72 ± 2.00 %). The increase in zeta potential and decrease in particle size were observed in co-precipitated samples, which explains their high solubility. Circular dichroism (CD) spectra results depict a change in the secondary structure of protein during the co-precipitation and HP treatment. The co-precipitation and HP treatment have significantly increased the invitro protein digestibility of millet and moringa leaf protein isolates. According to the findings, co-precipitation and HP treatment could be an effective method for enhancing the functional properties of millet and moringa protein.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"43 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142801
Saïd Abou El Karam, Anne Duconseille, Annie Vénien, Christine Ravel, Hugo Chauvet, Frederic Jamme, Matthieu Réfrégiers, Thierry Astruc
{"title":"Change in muscle fibre protein structure following salting process assessed by synchrotron deep UV fluorescence microspectroscopy","authors":"Saïd Abou El Karam, Anne Duconseille, Annie Vénien, Christine Ravel, Hugo Chauvet, Frederic Jamme, Matthieu Réfrégiers, Thierry Astruc","doi":"10.1016/j.foodchem.2025.142801","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142801","url":null,"abstract":"Samples of pork <em>teres major</em> muscle were salted and tumbled with 0.9 %, 1.3 % & 1.9 % sodium chloride respectively. The emission fluorescence (exc. 275 nm) of intramuscular connective tissue and of muscle fibre subtypes I, IIA and IIB-IIX was investigated by Synchrotron deep UV fluorescence microspectroscopy in order to characterize the change of the macromolecular structure of proteins. On emission spectra, tryptophan fluorescence was predominant and an additional peak assigned to dityrosine was detected around 395 nm. The fluorescence emission spectra vary depending on salt level both on intramuscular connective tissue and muscle fibres which subtypes were discriminated for one animal among the set of four. This result is encouraging in the perspective of developing sensors for meat evolution during the salting process. However, no noticeable prediction law linking the fibre autofluorescence to salt level could be deduced.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"117 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142810
Chao Ma, Tongyao Du, Wenxuan Wang, Yujia Liu, Zhenhong An, Qin Hou, Lujuan Xing, Wangang Zhang
{"title":"Insights into the differences of caspase and apoptosis levels in pork longissimus thoracis muscles with different tenderness: A perspective on S-nitrosylation modification","authors":"Chao Ma, Tongyao Du, Wenxuan Wang, Yujia Liu, Zhenhong An, Qin Hou, Lujuan Xing, Wangang Zhang","doi":"10.1016/j.foodchem.2025.142810","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142810","url":null,"abstract":"This study investigated the differences of caspase and apoptosis levels in pork with different tenderness from the perspective of S-nitrosylation and further explored their role during pork tenderization. Ten <em>longissimus thoracis</em> muscles selected from 36 individual carcasses based on shear force were divided into high (HT) and low (LT) tenderness groups (<em>n</em> = 5), respectively. Results demonstrated that total nitric oxide synthase activity and protein S-nitrosylation levels of LT group were higher than HT group, while myocyte apoptosis levels were lower in LT group (<em>p</em> < 0.05). Additionally, LT group possessed a lower caspase-3 activity while a higher abundance of intact caspase-3 and greater S-nitrosylation levels of cleaved caspase-3 (<em>p</em> < 0.05). However, none of the above differences were found in caspase-9 (<em>p</em> > 0.05). The lower tenderness in LT group might be associated with reduced caspase-3 activity resulted from increased S-nitrosylation levels of its active subunits, which delayed myocyte apoptosis and thereby lowed the degradation desmin and troponin T.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"203 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Magnetic graphene-enhanced exonuclease III assisted amplification strategy driven carbon nanozyme for tri-mode detection of Escherichia coli O157:H7","authors":"Yuwei Ren, Lulu Cao, Zongyang Li, Xin Zhang, Hui Li, Rui Jiao, Xiaoyu Yu, Zhao Liang, Ganghui Li, Xiyang Xia, Na Ling, Danfeng Zhang, Jianghua Cheng, Yingwang Ye","doi":"10.1016/j.foodchem.2025.142776","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142776","url":null,"abstract":"Ultra-precision point-of-care detection of <em>Escherichia coli</em> O157:H7 in foods is an important issue. Here, the detection sensitivity was improved by a signal cascade amplification strategy synergised by exonuclease III assisted isothermal amplification and reverse magnetic strategy. The double-stranded DNA formed by the aptamer and the target DNA as a sensing switch, avoiding the complex process of specific nucleic acid extraction. Further, the signal display element is a green nanozyme synthesized by co-doping Cu and Cl elements with lignin as precursor, which has excellent peroxidase activity and fluorescence properties. The detection limits of fluorescence, colorimetric, and photothermal modes were 6.1, 8.4, and 9.7 cfu/mL, respectively. In order to enhance the precision of cascade signal amplification and the portability of quantitative detection, we built a neural network BP model installed in a smartphone by measuring multi-component data, which provides flexible and selectable application scenarios for the on-site detection of foodborne pathogens.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"7 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142791
Tao Yang, Rui He, Qiuyu Xia, Ilaria Cacciotti, Sameh A. Korma, Weimin Zhang, Guohui Yi
{"title":"Phytosterol-enriched Camellia oleifera Abel seed oil obtained by subcritical butane extraction: Physicochemical properties and oxidative stability","authors":"Tao Yang, Rui He, Qiuyu Xia, Ilaria Cacciotti, Sameh A. Korma, Weimin Zhang, Guohui Yi","doi":"10.1016/j.foodchem.2025.142791","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142791","url":null,"abstract":"Tea (<em>Camellia oleifera</em> Abel) seed oil (TSO) has antioxidant and pharmacological properties. In this study, TSO was obtained from tea seeds by subcritical <em>n-</em>butane extraction (SBE), which is an environmentally friendly method. The oil yield, quality characteristics, and chemical composition of the extracted TSO were compared with those of oils obtained by supercritical carbon dioxide extraction (SCDE) and conventional cold pressing (CP). The TSO yield from SBE (51.35 %) was similar to that from SCDE (50.30 %), but higher than that from CP (31.82 %). TSO from SBE had better oxidative stability than that from SCDE and a lower toxic aldehyde content (0.41 mg/L) than that from SCDE (0.71 mg/L) and CP (2.67 mg/L). Moreover, TSO from SBE had relatively high tocopherols (26.20 mg/100 g) and phytosterols (842.25 mg/100 g) contents. Based on these findings, SBE is a promising method for obtaining high-quality TSO enriched with tocopherols and phytosterols.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"28 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142794
Guangzhi Wang, Yuchen Guo, Yang Yu, Yan Shi, Yuxiang Ying, Hong Men
{"title":"ColorNet: An AI-based framework for pork freshness detection using a colorimetric sensor array","authors":"Guangzhi Wang, Yuchen Guo, Yang Yu, Yan Shi, Yuxiang Ying, Hong Men","doi":"10.1016/j.foodchem.2025.142794","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142794","url":null,"abstract":"Pork freshness is crucial for flavour, nutrition and consumer health. The current colorimetric sensor array (CSA) detection systems face challenges related to high sensor development costs, low recognition accuracy and limitations in the platform use. Herein, we developed a CSA and ColorNet framework to detect pork freshness. The 53-point CSA was designed by selecting sensitised pH indicators and aldehyde/ketone indicators. To optimize the sensor, the Euclidean distance method was used to identify 24 array points with dyes that exhibited more sensitive responses. The ColorNet captured the color information of pork freshness, allowing real-time detection with a 99.5 % accuracy. For practical deployment and mobile applications, a refined 12-point CSA was developed using gradient activation mapping, maintaining a 99 % recognition rate, which is comparable with the 24-point CSA. The proposed CSA and model ensure consumer health and safety, providing strong technical support for quality monitoring and control in the pork industry.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A versatile reusable polymer-based sensor for aluminum analysis in various food matrices","authors":"Soner Çubuk, Pelin Kaplan, Belma Gjergjizi Nallbani, Ece Kök Yetimoğlu, Memet Vezir Kahraman","doi":"10.1016/j.foodchem.2025.142809","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142809","url":null,"abstract":"Within the scope of this study, a polymer-based optical sensor that can polymerize under UV radiation and produce fluorescence when suitable functional monomers and crosslinkers were prepared for aluminum determination in yogurt, soybean flour, and meat samples. Parameters such as operating range, pH, sensitivity, selectivity, determination limit, and foreign ion effect were thoroughly investigated to validate the developed method and characterize this polymer-based membrane. The designed sensor has wavelengths of 322 nm for fluorescence excitation and 356 nm for emission, respectively. The studies were done at pH 3.0, and the sensor had a reaction time of 20–40 s. Furthermore, the linear range of the study is between 7.41 × 10<sup>−9</sup>–1.11 × 10<sup>−7</sup> mol L<sup>−1</sup>, and the detection limit of the developed sensor was calculated as 2.09 × 10<sup>−9</sup> mol L<sup>−1</sup>.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"35 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Insights into potential flavor-active peptides and taste-related compounds in Longjing teas: A comparative study of ‘Longjing 43’ and ‘Qunti’ cultivars","authors":"Kangni Yan, Jiatong Wang, Mengxue Zhou, Qunhua Peng, Abdelkader Bassiony Mahmoud, Xue Bai, Susanne Baldermann, Xinbing Jiang, Shan Feng, Yan Wu, Jianyu Fu, Zhi Lin, Haipeng Lv, Jiang Shi","doi":"10.1016/j.foodchem.2025.142790","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142790","url":null,"abstract":"Longjing tea is well-known for its exceptional umami/mellow flavor, but the complexity of interactions among chemical compositions has impeded in-depth understanding. This study comprehensively compared the taste contributors in ‘Longjing 43’ (LJ43) and ‘Qunti’ (QT). Peptidomics revealed 865 identified water-soluble peptides in QT, and 497 in LJ43, with 44 umami peptides predicted. Potential umami peptide-T1R1/T1R3 complexes were further modeled using AlphaFold 3. LJ43 had higher levels of theanine (>18 mg/g), and glutamine (~4 mg/g) compared to QT. Similar amounts of flavan-3-ols (>150 mg/g), particularly EGCG (>60 mg/g) and ECG (>30 mg/g) were determined in LJ43 and QT. Moreover, Longjing teas' characterized flavor contributions by macromolecules (peptides), and small molecules (amino acids, catechins, saccharides, and Maillard reaction products) were integrated. These insights will greatly expand tea flavor chemistry and provide promising approaches promotion premium Longjing teas.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"65 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142795
Xin Ma, Shimin Wu
{"title":"Transformation of polycyclic aromatic hydrocarbons during frying stinky tofu","authors":"Xin Ma, Shimin Wu","doi":"10.1016/j.foodchem.2025.142795","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142795","url":null,"abstract":"Reductions in polycyclic aromatic hydrocarbon (PAH) concentrations have been observed during frying. However, transformation mechanisms of PAHs remain unclear. We hypothesize that PAHs may be oxidized into oxygenated polycyclic aromatic hydrocarbons (OPAHs) and other derivatives during frying. First, the levels of 24 PAHs and 12 OPAHs during frying stinky tofu were evaluated. Among the OPAHs, 9,10-anthraquinone exhibited the greatest increase in frying oil, with an increase rate of 55 %. The transformation of anthracene into 9,10-anthraquinone was further confirmed through stable isotope tracing. Targeted and untargeted mass spectrometry analyses suggested that anthrone, heavy PAHs, and alkyl derivatives were also produced from anthracene. Theoretical calculations revealed that radical addition was kinetically and energetically more favourable than hydrogen abstraction in the initial reaction of anthracene. This study systematically elucidates, for the first time, the transformation mechanisms of anthracene in frying oil, paving the way for reducing health risks associated with PAHs.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"99 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food ChemistryPub Date : 2025-01-06DOI: 10.1016/j.foodchem.2025.142799
Boya Lv, Yuanhao Xu, Peng Wu, Xiao Dong Chen
{"title":"Insights into the digestive processes of normal and high-amylose rice using realistic boluses formed in the bio-inspired oral mastication simulator (iBOMS-III)","authors":"Boya Lv, Yuanhao Xu, Peng Wu, Xiao Dong Chen","doi":"10.1016/j.foodchem.2025.142799","DOIUrl":"https://doi.org/10.1016/j.foodchem.2025.142799","url":null,"abstract":"Mastication is essential for preparing food bolus for swallowing and digestion. This study employed the bio-inspired oral simulator (iBOMS-III) to investigate the effects of mastication on physical properties and starch hydrolysis of normal rice (NR) and high-amylose rice (HR), while validating its results through comparison with <em>in vivo</em> data from human subjects. The median particle size (<em>d</em><sub>50</sub>) of NR (1.266, 0.931, 0.776 mm) and HR (1.32, 1.04, 0.928 mm) boluses from iBOMS-III at 12, 14, and 20 cycles closely matched human boluses at the swallowing threshold, particularly after 14 and 20 cycles. Starch hydrolysis in NR increased from 76.6 % to 91.3 %, while HR hydrolysis ranged from 63.4 % to 73.3 %. Boluses from iBOMS-III with 14 and 20 cycles showed starch digestibility consistent with <em>in vivo</em> data (84.2 % for NR, 71.3 % for HR). These findings demonstrate that iBOMS-III effectively replicated human mastication behaviors, producing boluses comparable to those observed <em>in vivo</em>.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"79 1","pages":""},"PeriodicalIF":8.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}