Z. Miladinov, I. Maksimović, S. Balešević-Tubić, V. Đukić, Z. Nikolić, B. Milošević, S. Katanski
{"title":"Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions","authors":"Z. Miladinov, I. Maksimović, S. Balešević-Tubić, V. Đukić, Z. Nikolić, B. Milošević, S. Katanski","doi":"10.5937/AASER2050105M","DOIUrl":"https://doi.org/10.5937/AASER2050105M","url":null,"abstract":"www.afc.kg.ac.rs Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions Zlatica Miladinov*1, Ivana Maksimović2, Svetlana Balešević Tubić1, Vojin Đukić1, Zorica Nikolić1, Branko Milošević1, Snežana Katanski1 1Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia 2Faculty of Agronomy, University of Novi Sad, Dositeja Obradovića 8, 21000 Novi Sad, Serbia *Corresponding author: zlatica.miladinov@ifvcns.ns.ac.rs Received 18 May 2020; Accepted 22 July 2020 A B S T R A C T Drought is one of the most important factors limiting the successful production of cultivated plants. One of the most sensitive stage to the water deficit in plants is seed germination. There are various methods of pre-sowing treatments that aim to reduce the negative impact of drought stress and improve seed germination. One of them is priming seeds. The aim of this experiment was to examine the effect of priming seeds in solutions KNO3 (1%), ascorbic acid AsA (100 mgl-1) and potassium chloride KCl (1%) on the reduction of the negative effect of drought stress. The effect of drought stress was simulated using different concentrations of PEG 6000 (0 (control), -0.30, -0.51, -0.80 MPa). The results of the research showed that with the increase of water deficiency, the effect of priming seeds is greater. At the water potential of the solution of -0.30 MPa, germination energy and seed germination increased on average by 6.77% and 5.08%, while at the water potential of the solution of -0.80 MPa, the increase was 19.28% and 16.75%, respectively. Also, priming seeds significantly reduced the intensity of lipid peroxidation and the content of free proline. From all the above, it can be concluded that priming of seeds is a method that can serve to improve the germination of soybean seeds in conditions of drought stress.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"25 1","pages":"105-111"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hakeem, M. Waseem, Baloch Mohammad, G. Khaliq, Shahab Anwer, M. Tahir, Q. Ali, M. Khan
{"title":"Planting scale effect as the indicator of sesame yield under coastal conditions","authors":"M. Hakeem, M. Waseem, Baloch Mohammad, G. Khaliq, Shahab Anwer, M. Tahir, Q. Ali, M. Khan","doi":"10.5937/aaser2049003h","DOIUrl":"https://doi.org/10.5937/aaser2049003h","url":null,"abstract":"A field experiment was conducted to evaluate the effect of planting scale on sesame yield under coastal conditions during 2016 at the Lasbela University of Agriculture, Water and Marine Science, Uthal, Balochistan. The trial treatment included three sesame varieties i.e. SV1 (‘TS-5’), SV2 (‘TH-6’) and SV3 (‘4002’) grown at three sowing dates at 15 days’ intervals, S1 = 1st sowing (15 March 2016), S2 = 2nd sowing (1 April 2016) and S3 = 3rd sowing (15 April 2016). The results of various growth and yield attributes were found to be significant, and a non-significant interaction was found for sowing dates and sesame genotypes. Maximum yield was observed at S3= 3rd sowing date (15 April 2016) for SV1 (‘TS-5’) under the applied treatment. Testing under the coastal environmental conditions of the Lasbela District suggested that maximum seed weight was obtained by planting the sesame variety ‘TS-5’ on 15 April 2016.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction: Quality management system in trade: Internationalisation of marketing relations with consumers (2014, vol. 19, no. 37, p. 15-26)","authors":"Board Editorial","doi":"10.5937/aaser2050199e","DOIUrl":"https://doi.org/10.5937/aaser2050199e","url":null,"abstract":"In the article Quality Management System in Trade - Internationalisation of Marketing Relations with Consumers, published in Acta Agriculturae Serbica, Vol. XIX, 37 (2014), Katarina Marković was accidentally listed as a co-author and her affiliation was mistakenly associated with Gvozden Jovanović, the co-author listed first. Since the paper was single authored, only Gvozden Jovanović's name should remain and be affiliated to: Faculty of Business Studies, Megatrend University, Bulevar Maršala Tolbuhina 8, 11070 Belgrade, Serbia. The author has explained that the mistake was due to misunderstanding and carelessness; therefore, although the journal is not responsible for the mistake, we hereby apologise to our readers.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Body condition as an indicator of cow welfare","authors":"M. Lalović, Tatjana Krajišnik, Nikolina Mašić","doi":"10.5937/AASER2050187L","DOIUrl":"https://doi.org/10.5937/AASER2050187L","url":null,"abstract":"www.afc.kg.ac.rs Body condition as an indicator of cow welfare Miroslav Lalović, Tatjana Krajišnik*, Nikolina Mašić University of East Sarajevo, Faculty of Agriculture, Vuk Karadžić 30, East Sarajevo, Bosnia and Herzegovina *Corresponding author: tatjana.krajisnik@pof.ues.rs.ba Received 27 June 2020; Accepted 1 December 2020 A B S T R A C T The main objective of this research was to determine the quality level of welfare of milk cows at various stages of lactation (the first 60 days of the lactation period and the dry period) and of different breeds (Holstein-Friesian and Simmental) on farms (A, B, C, D) with different systems of housing (free and tie-stall), based on nutritional status as a direct individual indicator of wellbeing. Results showed that the body condition scores of cows were statistically very highly significantly (p<0.001) influenced by breed, production stage and the breed x production stage interaction; statistically highly significantly (p<0.01) by the breed x housing system interaction, and significantly (p<0.05) by the housing system x production stage interaction.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"13 1","pages":"187-192"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Hariri, N. Ouis, D. Bouhadi, Zouaoui Benatouche
{"title":"In vitro antioxidant activity of essential oil of aerial parts of Mentha pulegium L","authors":"A. Hariri, N. Ouis, D. Bouhadi, Zouaoui Benatouche","doi":"10.5937/AASER2050193H","DOIUrl":"https://doi.org/10.5937/AASER2050193H","url":null,"abstract":"This work was conducted to evaluate the antioxidant activity of the essential oil obtained from the aerial part of Mentha pulegium. The antioxidant power of the essential oil obtained by hydrodistillation using a Clevenger-type apparatus was evaluated by using three methods: free radical-scavenging activity, reducing power and liver lipid peroxidation assay. Results showed that Mentha pulegium oil displayed good quality according to its physicochemical characteristics, and a higher yield 5.1 ± 0.2%. The essential oil of Mentha pulegium showed a higher DPPH radical scavenging activity 90.54 ± 1.5 % at a concentration of 1000 µg/mL. This value was close to the results obtained with ascorbic acid 96.23 ± 1.2%, and catechin 94.50 ± 1.4%. This oil exhibited significant potential for reducing iron (the value observed by optical density was 1.8 ± 0.3), while ascorbic acid and catechin provided an OD of 2.069 ± 0.03 and 2.66 ± 0.016 at the same concentration of 1000 µg/mL. The tested oil protected against lipid peroxidation induced by Fe+2, and considerably increased the percentage of anti lipid peroxidation in a dose-dependent manner. The studied oil displayed a good degree of antioxidant activity and can be exploited in food and pharmaceutical industries.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"25 1","pages":"193-197"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sanitary assessment of soil in the municipality of Čačak based on the presence of Escherichia coli and Salmonella sp.","authors":"L. Mandić, D. Đukić, M. Pešaković, Vesna Đurović","doi":"10.5937/aaser2049065m","DOIUrl":"https://doi.org/10.5937/aaser2049065m","url":null,"abstract":"The data obtained indicate that the examined soils are mostly unpolluted or slightly to moderately polluted and that therefore they will not have a negative effect on plant production and the health of humans and animals. soils near industrial zones and landfills, soils close to congested roads,soils exposed to floods, and soils exposed to the uncontrolled use of agrochemicals and agricultural machinery.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Cvijanović, N. Čolic, Nenad Đurić, G. Dozet, Abduladim Eltreki, Marija Cvijanović, M. Žuža
{"title":"Effect of biostimulants on soybean seedlings","authors":"G. Cvijanović, N. Čolic, Nenad Đurić, G. Dozet, Abduladim Eltreki, Marija Cvijanović, M. Žuža","doi":"10.5937/AASER2050099C","DOIUrl":"https://doi.org/10.5937/AASER2050099C","url":null,"abstract":"www.afc.kg.ac.rs Effect of biostimulants on soybean seedlings Gorica Cvijanović1, Ninoslav Čolić2, Nenad Đurić2, Gordana Dozet2, Abduladim Eltreki2, Marija Cvijanović3, Milena Žuža2 1University of Kragujevac, Institute for Information Technologies Jovana Cvijića bb, Kragujevac, Serbia 2Megatrend University, Faculty of Biofarming Maršala Tita 39, 24300 Bačka Topola, Serbia 3University of Bijeljina, Faculty of Agriculture Pavlovića put bb, 76300 Bijeljina, BiH *Corresponding author: cvijagor@yahoo.com Received 8 April 2020; Accepted 9 September 2020 A B S T R A C T The aim of this study was to analyze the effect of biostimulants on the morphological characteristics of soybean seedlings. The testing was conducted in the laboratory of the Faculty of Biofarming in Bačka Topola. The experimental material included three soybean varieties (‘Galina’, ‘Sava’ and ‘Rubin’) selected at the Institute of Field and Vegetable Crops in Novi Sad. The study lasted for two years, 2015–2016, and identical biostimulant treatments were applied in both years. In order to determine the effect of biostimulants on soybean seedling root, hypocotyl and weight, the following commercial biostimulants were applied: EM Aktiv, Terra Green Hobby, Slavol and Bioplant Flora. In addition to the single application of biostimulants, two combinations of Slavol + Bioplant Flora and Slavol + Bioplant Flora + Epin Extra + Slavol S were used as treatments. EM Aktiv showed the greatest effect on root growth. The root was on average 12% longer than the control. Slavol S had the greatest influence on seedling hypocotyl and weight. The increase was 8.24% and 5.15%, respectively, compared with the control.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"25 1","pages":"99-104"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Matović, V. Kurćubić, Radojica D. Đoković, M. Petrović, M. Šekler, Bojana Tešović
{"title":"Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia","authors":"K. Matović, V. Kurćubić, Radojica D. Đoković, M. Petrović, M. Šekler, Bojana Tešović","doi":"10.5937/aaser2049083m","DOIUrl":"https://doi.org/10.5937/aaser2049083m","url":null,"abstract":"Viral and bacterial agents causing bovine respiratory disease complex (BRDC) were examined over a five-year period (2015– 2019). Thirty-eight farms of beef and dairy cattle from Central Serbia which showed symptoms of BRDC were observed. A total of 118 nasal swab specimens collected from diseased bullocks (n=55), cows (n=18) and calves (n=45) were chosen for the isolation of bacteria and identification by aerobic cultivation. The most commonly isolated bacterial pathogen was Pasteurella multocida (72.88% positive samples). Disk-diffusion method (antibiogram) showed that Amoxiclav and Enrofloxacin were the most efficient antibiotics against Pasteurella multocida isolates (66.67% and 54.17%, respectively). From all examined samples, using the Real Time RT-PCR and PCR methods, we determined the genome sequences of bovine respiratory syncytial virus (BRSV– 34.78% of tested samples (8/23), bovine viral diarrhea virus (BVDV, in 34.37% (11/32) and Mycoplasma spp. in 33.33% (8/24). The genome of BoHV-1 virus was not detected in any of the 19 samples tested by Real Time PCR method.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Vujović, D. Jevremović, T. Marjanović, I. Glišić
{"title":"In vitro propagation and medium-term conservation of autochthonous plum cultivar 'Crvena Ranka'","authors":"T. Vujović, D. Jevremović, T. Marjanović, I. Glišić","doi":"10.5937/AASER2050141V","DOIUrl":"https://doi.org/10.5937/AASER2050141V","url":null,"abstract":"Vujović et al. 142 (Glišić, 2015), ranging from autochthonous to newly bred ones. Indigenous cultivars are part of the Serbian tradition, customs, legacy, and cultural identity. In addition to providing the genetic basis for clonal selection, they are also used in different breeding programs aimed at developing new plum cultivars as well as new plum, apricot and peach rootstocks (Milošević et al., 2010). However, the majority of these cultivars are being seriously threatened and are slowly disappearing from orchards. Among autochthonous cultivars, ‘Crvena Ranka’ stands out as a sharka-tolerant cultivar mostly used for producing supreme quality plum brandies (Popović et al., 2015). Fruits of some local genotypes are suitable for fresh use (Milošević and Milošević, 2012). In recent years, there has been an increased interest in establishing new commercial orchards of this valuable cultivar. Therefore, it is of vital importance to develop protocols for the clonal propagation of selected genotypes to obtain a large number of true-to-type plants from a few initial plants, in the shortest period of time. The rapid production of highquality, disease-free and uniform planting stock is only possible through micropropagation. However, long-term successive subculture of in vitro plants on a fresh medium and their maintenance under normal growth conditions can be followed by a decrease in or loss of the cultures’ morphogenetic potential as well as by an increase in the possibility of genetic alterations or propagating material loss due to human errors or microbial contamination (Chauhan et al., 2019). On the other hand, tissue culture technology also enables the conservation of plant genetic resources for either short, medium or long term, depending on the requirement as well as on the technique applied (Engelmann, 1997). In vitro conservation of vegetatively propagated species such as fruit tree species is complementary to field gene banks, which, along with in situ conservation measures, provide an integrated conservation strategy (Rajasekharan and Sahijram, 2015). The aim of this paper was to establish an efficient protocol for the in vitro propagation of autochthonous plum cultivar ‘Crvena Ranka’ by optimizing multiplication and rooting stages, and to examine the possibility of mid-term conservation of this genotype using the slow growth storage method, which involved temperature reduction pooled with the maintenance of cultures under dark conditions. 2. Material and methods Plant material and establishment of aseptic culture A selected clone of autochthonous plum cultivar ‘Crvena Ranka’ (Prunus domestica L.) originating from Gledić Mountains was used as the source of initial explants for in vitro culture. Aseptic culture was established using actively growing axillary leaf buds taken from branches during the spring. The surface sterilization procedure involved washing explants under running water for 2 h, sterilization in 70% ethanol (1 min), and 15 min","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"25 1","pages":"141-147"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Grujović, K. Mladenović, V. Jakovljević, L. Čomić
{"title":"Detection of enzymes produced by lactic acid bacteria isolated from traditionally made Serbian cheese and their role in the formation of its specific flavor","authors":"M. Grujović, K. Mladenović, V. Jakovljević, L. Čomić","doi":"10.5937/AASER2050165G","DOIUrl":"https://doi.org/10.5937/AASER2050165G","url":null,"abstract":"И З В О Д Циљ овог рада био је испитивање способности продукције ензима девет одабраних врста (16 изолата) бактерија млечне киселине (БМК) изолованих из српског сира произведеног на традиционалан начин, како би се утврдило који аутохтони сојеви имају технолошки утицак у процесу производње сира. Изолати су одабрани на основу претходно утврђених биохемијских и физиолошких карактеристика, као и антимикробне активности и идентификовани су као: Lactococcus lactis subsp. lactis (један изолат), Lc. lactis subsp. lactis biovar. diacetylactis (пет изолата), Lactobacillus fermentum (два изолата), Lb. plantarum (један изолат), Lb. brevis (један изолат), Enterococcus faecalis (три изолата), E. faecium (један изолат), E. durans (један изолат) и E. hirae (један изолат). Ензимска активност, која је обухватала детекцију киселе и алкалне инвертазе, алкалне фосфатазе, алкалне протеасе и α-амилазе, је одређена коришћењем спектрофотометра. Резултати указују да су сви изолати Lactobacillus показали активност протеазе, амилазе и алкалне фосфатазе, док активност киселе и алкалне инвертазе није примећена. Изолати лактокока су показали активност протеазе, киселе инвертазе и алкалне фосфатазе, осим изолата KGPMF50, који није показао активност алкалне фосфатазе. Испитани изолати ентерокока показали су слабу и изолат специфичну ензиматску активност. Резултати указују да продуковани ензими испитиваних изолата имају улогу у формирању специфичног укуса сира и да сви изолати, посебно изолати из рода Lactobacillus, показују потенцијал за примену у млечној индустрији или у примењеној биотехнологији. Кључне речи: ензимска активност, бактерије млечне киселине, традиционална произведња сира, биотехнологија, млечна индустрија.","PeriodicalId":31632,"journal":{"name":"Acta Agriculturae Serbica","volume":"25 1","pages":"165-169"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71193605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}