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Pathway Activation Strength (PAS) analysis of microarray data reveal similarities of aging with age-related macular degeneration and Hutchinson-Gilford progeria ? should aging be considered as a disease? 微阵列数据的通路激活强度(PAS)分析揭示了衰老与年龄相关性黄斑变性和Hutchinson-Gilford早衰症的相似性。衰老应该被视为一种疾病吗?
Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31151
Eugene Makarev, A. Zhavoronkov, A. Aliper
{"title":"Pathway Activation Strength (PAS) analysis of microarray data reveal similarities of aging with age-related macular degeneration and Hutchinson-Gilford progeria ? should aging be considered as a disease?","authors":"Eugene Makarev, A. Zhavoronkov, A. Aliper","doi":"10.5281/ZENODO.31151","DOIUrl":"https://doi.org/10.5281/ZENODO.31151","url":null,"abstract":"Aging is a complex process leading to loss of body and cognitive functions as well as to several age-related diseases. The complexity of human aging has eluded biologists and physicians for decades, leading to a concerted effort to unravel the physiological, cellular and molecular mechanisms of aging. A potentially successful approach involves the analysis of naturally occurring aging disorders [1,2]. For instance, age-related macular degeneration (AMD) is a major cause of blindness in older people with age as the main risk factor (1). Premature aging is particularly manifested in the rare genetic condition, Hutchinson-Gilford Progeria Syndrome (HGPS) (2), which is a disease with major phenotypic features of accelerated aging. However, the contribution of aging to these age-related diseases is still poorly understood. In this research study we compared different tissue-specific pathway activation profiles in normal aging with progeria and AMD. We used GeroScope, a bioinformatics platform that enables calculation of the Pathway Activation Strength (PAS) (3), for qualitative measurement of pathway activation in a relevant set of microarray gene expression data representing aged, AMD and HGPS tissue samples. Here we present data supporting the notion that these age-related diseases share similarity with healthy aging at the signaling pathway level. Therefore, targeting aging-related signaling pathways may be a valuable avenue to identify cures for a variety of age-related diseases, including AMD, HGPS, Alzheimer’s disease and others.","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127574398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel, microstructured, polymer-based metal enhance fluorescence (MEF) substrate using established industrial molding and coating processes derived from Blu-ray disc manufacturing for the use in ultra-sensitive fluorescence immunoassays 一种新型的微结构聚合物基金属增强荧光(MEF)基板，采用源自蓝光光盘制造的成熟工业成型和涂层工艺，用于超敏感荧光免疫测定
Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31132
G. Hawa
{"title":"A novel, microstructured, polymer-based metal enhance fluorescence (MEF) substrate using established industrial molding and coating processes derived from Blu-ray disc manufacturing for the use in ultra-sensitive fluorescence immunoassays","authors":"G. Hawa","doi":"10.5281/ZENODO.31132","DOIUrl":"https://doi.org/10.5281/ZENODO.31132","url":null,"abstract":"","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133767517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
SiMPLInext: the next generation of well-plate inserts for predictive model of biological barriers SiMPLInext:下一代孔板插入物，用于生物屏障的预测模型
Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31162
Silvia Angeloni Suter
{"title":"SiMPLInext: the next generation of well-plate inserts for predictive model of biological barriers","authors":"Silvia Angeloni Suter","doi":"10.5281/ZENODO.31162","DOIUrl":"https://doi.org/10.5281/ZENODO.31162","url":null,"abstract":"","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"145 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133908392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased SPR-sensitivity enables fragment screening and kinetic characterization at lower surface densities 增加的spr灵敏度可以在较低表面密度下进行碎片筛选和动力学表征
Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/ZENODO.31134
U. Roder
{"title":"Increased SPR-sensitivity enables fragment screening and kinetic characterization at lower surface densities","authors":"U. Roder","doi":"10.5281/ZENODO.31134","DOIUrl":"https://doi.org/10.5281/ZENODO.31134","url":null,"abstract":"Aldose Reductase (AR) is a 36 kDa NADPHdependent enzyme involved in glucose metabolism It is a potential target for the prevention of diabetic complications. 3D structural analysis had shown that six selected compounds could be divided into two groups based on mode of action; one group left the hydrophobic pocket closed and the other group opened the binding pocket. The purpose of this study was to elucidate if the difference between the two groups is reflected in their binding kinetics. Introduction","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127345227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Patch them all - fully HTS-compatible automated patch clamping of 384 cells at once for massively parallel ion channel screening 将它们全部贴片-完全兼容hts的384个细胞的自动贴片夹紧，一次进行大规模平行离子通道筛选
Basel Life Science Week Pub Date : 2015-09-18 DOI: 10.5281/zenodo.31139
Ilka Rinke
{"title":"Patch them all - fully HTS-compatible automated patch clamping of 384 cells at once for massively parallel ion channel screening","authors":"Ilka Rinke","doi":"10.5281/zenodo.31139","DOIUrl":"https://doi.org/10.5281/zenodo.31139","url":null,"abstract":"","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126970644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of high throughput electric field stimulation (EFS) coupled with intracellular Ca2+ kinetics measurements on iPSC-derived cardiomyocytes 使用高通量电场刺激(EFS)与细胞内Ca2+动力学测量ipsc衍生的心肌细胞
Basel Life Science Week Pub Date : 2015-09-17 DOI: 10.5281/ZENODO.31125
J. D'Angelo
{"title":"Use of high throughput electric field stimulation (EFS) coupled with intracellular Ca2+ kinetics measurements on iPSC-derived cardiomyocytes","authors":"J. D'Angelo","doi":"10.5281/ZENODO.31125","DOIUrl":"https://doi.org/10.5281/ZENODO.31125","url":null,"abstract":"","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122408815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optogenetics: a bright future for voltage gated ion channels 光遗传学:电压门控离子通道的光明前景
Basel Life Science Week Pub Date : 2015-09-17 DOI: 10.5281/ZENODO.31126
J. D'Angelo
{"title":"Optogenetics: a bright future for voltage gated ion channels","authors":"J. D'Angelo","doi":"10.5281/ZENODO.31126","DOIUrl":"https://doi.org/10.5281/ZENODO.31126","url":null,"abstract":"Channelrhodopsin-2 (ChR2) is a light-activated microbial cation channel which can be used to depolarize neurons through the incidence of blue light (470 nm). The possibility to optically control the plasma membrane voltage opens new and interesting perspectives for the characterization of voltage-gated ion channels and the search for modulators. Proof of principle studies have been performed to verify the applicability of this tool for the development of cell based assays in High Throughput Screening (HTS) platforms as microplate readers. An HEK-293 cell line, which stably co-expresses the human voltage-gated calcium channel hCav1.3 and the inward rectifi er hKir2.3 channel, was over-transfected with a ChR2 carrying a single amino acids mutation. This latter results in a prolonged lifetime of the conducting state of ChR2 and in a reduced light power requirement for its activation. A protocol of light stimulation of ChR2 and record of calcium ion infl ux through Cav1.3 with the use of a calcium-sensitive fl uorescent dye (Fluo8) was tested in the Hamamatsu FDSSµcell. A well-known Cav1.3 blocker, Isradipine, tested in the resting and the partial inactivated Cav1.3 states, was used to confi rm the pharmacological profi le. Data obtained for the ChR2/hCav1.3 cell line by light stimulation have been also compared to the extracellular potassium stimulus and to patch-clamp to cross-check their reliability. Key-questions of the study Is the blue light produced by the FDSSµcell LED intense enough to activate ChR2? ➔ Can the light protocol be runned with the FDSSµcell? Is the activation of ChR2 suffi cient to induce cell membrane depola-rization? ➔ Can the ChR2 be used to depolarize cells avoiding the irreversible depolarization protocols using KCl injection? Is the resulting membrane depolarization suffi cient to activate the transfected voltage-gated channel Cav1.3? Can the cation fl ux through ChR2 disturbe the detction of Ca 2+ fl ux through the transfected target Cav1.3? Dihydropyridine Phenylalkylamine Benzothiazepin Membrane repolarization Time course of membrane repolarization after a fi rst blue light pulse (MPdye)","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"179 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134439938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
BRET2 assay using the FDSS/?CELL: monitoring cell surface-receptor internalization and intracellular trafficking 使用FDSS/?细胞:监测细胞表面受体内化和细胞内运输
Basel Life Science Week Pub Date : 2015-09-17 DOI: 10.5281/ZENODO.31127
J. D'Angelo
{"title":"BRET2 assay using the FDSS/?CELL: monitoring cell surface-receptor internalization and intracellular trafficking","authors":"J. D'Angelo","doi":"10.5281/ZENODO.31127","DOIUrl":"https://doi.org/10.5281/ZENODO.31127","url":null,"abstract":"(a) At basal state, over-expression of the two partners and their common location on plasma membrane will allow a relative proximity and a certain energy transfer. The activation of the receptor will trigger its internalization inducing an increase of the distance between the partners and a decrease of BRET. (b) At basal state, distance is such that the BRET signal is low. Once activated, the receptor is relocated to endosomes inducing a relative proximity and an increase of BRET.","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129465719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electric field stimulation (EFS) of human iPSC-derived neuron using Hamamatsu FDSS/?CELL Hamamatsu FDSS/?对人ipsc衍生神经元的电场刺激细胞
Basel Life Science Week Pub Date : 2015-09-17 DOI: 10.5281/zenodo.31128
J. D'Angelo
{"title":"Electric field stimulation (EFS) of human iPSC-derived neuron using Hamamatsu FDSS/?CELL","authors":"J. D'Angelo","doi":"10.5281/zenodo.31128","DOIUrl":"https://doi.org/10.5281/zenodo.31128","url":null,"abstract":"","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120947061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Baculovirus expression vectors for optimal recombinant protein and virus-like particle production 杆状病毒表达载体的优化重组蛋白和病毒样颗粒的生产
Basel Life Science Week Pub Date : 2015-09-16 DOI: 10.5281/zenodo.31030
A. Chambers, Benjamin De Glanville, L. King, R. Possee
{"title":"Baculovirus expression vectors for optimal recombinant protein and virus-like particle production","authors":"A. Chambers, Benjamin De Glanville, L. King, R. Possee","doi":"10.5281/zenodo.31030","DOIUrl":"https://doi.org/10.5281/zenodo.31030","url":null,"abstract":"Figure 1. (1) A recombinant baculovirus is produced by simply co-transfecting insect cells with the flashBAC virus DNA and a suitable transfer vector containing ‘the gene(s) under investigation. (2) Homologous recombination within the insect cells restores the function of an essential gene that is partly deleted in flashBAC (ORF1629), allowing the flashBAC virus DNA to replicate and produce virus particles. This also simultaneously inserts ‘the gene(s) under investigation’ into the virus DNA under the control of the polyhedrin promoter. (3) The recombinant virus genome, with the restored essential gene, replicates to produce baculovirus that can be harvested from the culture medium of the transfected insect cells that forms a seed stock of recombinant virus. As it is not possible for non-recombinant virus to replicate there is no need for any selection system. • flashBACULTRA viruses were generated as described in Figure 1, each containing gene(s) that expressed protein(s), which self-assemble into VLPs.","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115314946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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