Increased SPR-sensitivity enables fragment screening and kinetic characterization at lower surface densities

Abstract

Aldose Reductase (AR) is a 36 kDa NADPHdependent enzyme involved in glucose metabolism It is a potential target for the prevention of diabetic complications. 3D structural analysis had shown that six selected compounds could be divided into two groups based on mode of action; one group left the hydrophobic pocket closed and the other group opened the binding pocket. The purpose of this study was to elucidate if the difference between the two groups is reflected in their binding kinetics. Introduction