BRET2 assay using the FDSS/?CELL: monitoring cell surface-receptor internalization and intracellular trafficking

Abstract

(a) At basal state, over-expression of the two partners and their common location on plasma membrane will allow a relative proximity and a certain energy transfer. The activation of the receptor will trigger its internalization inducing an increase of the distance between the partners and a decrease of BRET. (b) At basal state, distance is such that the BRET signal is low. Once activated, the receptor is relocated to endosomes inducing a relative proximity and an increase of BRET.