{"title":"使用FDSS/?细胞:监测细胞表面受体内化和细胞内运输","authors":"J. D'Angelo","doi":"10.5281/ZENODO.31127","DOIUrl":null,"url":null,"abstract":"(a) At basal state, over-expression of the two partners and their common location on plasma membrane will allow a relative proximity and a certain energy transfer. The activation of the receptor will trigger its internalization inducing an increase of the distance between the partners and a decrease of BRET. (b) At basal state, distance is such that the BRET signal is low. Once activated, the receptor is relocated to endosomes inducing a relative proximity and an increase of BRET.","PeriodicalId":315352,"journal":{"name":"Basel Life Science Week","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"BRET2 assay using the FDSS/?CELL: monitoring cell surface-receptor internalization and intracellular trafficking\",\"authors\":\"J. D'Angelo\",\"doi\":\"10.5281/ZENODO.31127\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"(a) At basal state, over-expression of the two partners and their common location on plasma membrane will allow a relative proximity and a certain energy transfer. The activation of the receptor will trigger its internalization inducing an increase of the distance between the partners and a decrease of BRET. (b) At basal state, distance is such that the BRET signal is low. Once activated, the receptor is relocated to endosomes inducing a relative proximity and an increase of BRET.\",\"PeriodicalId\":315352,\"journal\":{\"name\":\"Basel Life Science Week\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Basel Life Science Week\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5281/ZENODO.31127\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basel Life Science Week","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5281/ZENODO.31127","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
BRET2 assay using the FDSS/?CELL: monitoring cell surface-receptor internalization and intracellular trafficking
(a) At basal state, over-expression of the two partners and their common location on plasma membrane will allow a relative proximity and a certain energy transfer. The activation of the receptor will trigger its internalization inducing an increase of the distance between the partners and a decrease of BRET. (b) At basal state, distance is such that the BRET signal is low. Once activated, the receptor is relocated to endosomes inducing a relative proximity and an increase of BRET.
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