German Journal of Veterinary Research最新文献

{"title":"Isolation and molecular characterization of Foot and Mouth Disease virus serotype O circulated in Kenya during the period 2013-2018","authors":"E. Chepkwony, G. Gitao, G. Muchemi, A. Sangula","doi":"10.51585/gjvr.2022.4.0047","DOIUrl":"https://doi.org/10.51585/gjvr.2022.4.0047","url":null,"abstract":"The endemicity of Foot-and-mouth disease (FMD) in Kenya has been recognized for over a century, with the first recorded cases dating back to 1915. Production of effective vaccines against incursions of infection in endemic areas is achieved by evaluating the genetic and antigenic characteristics of the circulating viruses. The present study aimed to isolate, serotype, and molecularly characterize FMDV from Kenya from 2013-2018. Isolation was done from 58 field samples on BHK-21 cells, and serotyping of the isolated viruses was carried out using antigen ELISA. Isolated viruses were also analyzed using reverse transcription PCR, and the PCR products were subjected to sequencing. Based on the quality of obtained sequence spectra, only 51 isolates were aligned using MEGA v11.0.8, employing the ClustalW algorithm. SeaView version 5.0.4 was used to edit the alignment, and MEGA 11.0.8 was used to construct the phylogenetic tree and align it with the commercially used vaccinal strains (OK77/78 and OK82/98). With a few exceptions, isolates collected over the same period and those from the same regions consistently clustered in the same lineage or closer to each other. A total of 50/51 strains belong to the East African-2 (EA-2) topotype together with the vaccine strain OK82/98. However, only one strain (1/51) isolated from Tana River county belongs to the EA-1 topotype together with the current vaccine strain (OK77/78). None of these isolates was found to belong to the EA-and EA-4 topotypes. This study emphasizes the importance of regular surveillance and characterization of circulating virus strains for developing effective vaccines against FMD. It’s proposed that future vaccine candidate strains selection could consider EA-2 topotype strains to control FMDV circulating in Kenya.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121304015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antimicrobial susceptibility profiles of Campylobacter coli isolated from broilers and layers cloacal swabs in Mwanza and Arusha, Tanzania","authors":"R. Nshama, Abdul A. S. Katakweba, I. Kashoma, Noel Gahamanyi, E. Komba","doi":"10.51585/gjvr.2022.4.0045","DOIUrl":"https://doi.org/10.51585/gjvr.2022.4.0045","url":null,"abstract":"Campylobacteriosis is an emerging zoonotic enteric disease that poses a threat to both human health and animal productivity. Poultry is known as the primary reservoir of Campylobacter, and 90% of human Campylobacteriosis is caused by Campylobacter jejuni and Campylobacter coli. This is complicated by the worldwide emergence of Campylobacter strains that are resistant to commonly used antimicrobial compounds. In this study, we determined the prevalence and antimicrobial susceptibility profiles of Campylobacter coli isolated from cloacal swabs collected from broilers and layers in Mwanza and Arusha, Tanzania. We collected 402 cloacal swabs from broilers and layers. Then, samples were enriched into Bolton Broth supplemented with 5% laked horse blood. Campylobacter was isolated and confirmed by PCR. Antibiogram was done by disk diffusion method using six antibiotics i.e., ampicillin, nalidixic acid, gentamycin, erythromycin, tetracycline, and ciprofloxacin. Of the 402 samples, 31 (7.71%) were confirmed to be Campylobacter coli by PCR. In Mwanza, the overall prevalence was 6.5% (6% and 7% in broilers and layers, respectively), while in Arusha, the overall prevalence was 8.9% (10.8% and 7% in broilers and layers, respectively). Antimicrobial susceptibility testing showed that 80.6%, 16.1%, 9.7%, 9.7%, 6.5%, and 3.2% were resistant to ampicillin, nalidixic acid, erythromycin, tetracycline, ciprofloxacin, and gentamycin, respectively. The rate of antimicrobial resistance (AMR) to at least one antimicrobial was 100%. Eight out of thirty-one (12.9%) isolates were multi-drug-resistant to four different antimicrobial compounds, each with different patterns. Wise use of existing antimicrobials is necessary to curb the increasing trend of AMR strains.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114494361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The objectivity of vertebral heart scale: Comparison of detailed radiographic findings with vertebral heart scale in dogs with symptomatic cardiac failure","authors":"S. U. Saka, Onur Iskefli, M. Or, U. Bakirel","doi":"10.51585/2022.3.36","DOIUrl":"https://doi.org/10.51585/2022.3.36","url":null,"abstract":"The current study aimed to evaluate the objectivity of the vertebral heart scale in the right, left, and generalized cardiomegaly. The correlation between vertebral heart scale and cardiomegaly was determined by conventional methods using a detailed evaluation form of vertebral heart scale and main stem bronchus spine distance measurements and radiographic findings. For this reason, a detailed radiographic evaluation form, including cardiac shape alterations, tracheal displacement, the ratio of the width of the heart to the thorax, and effusive findings due to cardiac failure, was compiled to provide gradation and detection of both-sided alterations in latero-lateral and dorso-ventral view. X-rays of 189 symptomatic dogs with cardiac disorders were included in the study and evaluated by experienced staff. X-ray findings were classified as left-right and both-sided enlargement and graded as mild, moderate, or severe enlargement. Using statistical analysis, the vertebral heart scale method was found to be correlated with findings of left and generalized enlargement, and a significant difference was found between the moderate and severe class (respectively P<0.001 and P<0.000); whereas no significance was found in the group or intergroup findings in right-sided enlargements. Accordingly, apart from left-sided and generalized cardiomegaly, the vertebral heart scale measurements were found within normal limits in the dogs with right-sided enlargement.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116150488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking the diversity and Mediterranean lineage of Brucella melitensis isolates from different animal species in Turkey using MLVA-16 genotyping","authors":"Kadir Akar, Farah Tatar, G. Schmoock, G. Wareth, H. Neubauer, O. Erganis","doi":"10.51585/gjvr.2022.1.0037","DOIUrl":"https://doi.org/10.51585/gjvr.2022.1.0037","url":null,"abstract":"Brucellosis is a zoonotic disease with a high prevalence in humans and farm animals in Turkey. However, data on the genetic diversity of Brucella spp. circulating in Turkey and parts of the Mediterranean region are limited. In the present study, the genetic diversity of 50 B. melitensis isolates from seven regions of Turkey was investigated using multi-locus variable number tandem repeats analysis (MLVA-16). The profiles were compared with 163 B. melitensis isolates recovered from the Mediterranean basin. B. melitensis strains from Turkey contain 46 different genotypes and consist of two main clusters. B. melitensis isolates from Turkey were closely related to isolates from Greece and some Portuguese strains. The same genotypes isolated from different sites show the spread between sites. Therefore, uncontrolled animal movements and the trade of imported animals can be important factors for the spread of brucellosis. The endemic occurrence of B. melitensis in the Mediterranean basin is a result of socio-historical links between Mediterranean countries. Turkish strains belong to the Eastern Mediterranean line. Eradicating brucellosis in countries of the Mediterranean basin with high prevalence is a demanding need to reduce trade barriers and, more importantly, prevent human suffering","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117061872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine brucellosis serological survey in small dairy herds in Lushnja district, Albania","authors":"J. Arla, Muça Gerald, Koni Anita, Turmalaj Luigj, Koleci Xhelil","doi":"10.51585/gjvr.2022.1.0038","DOIUrl":"https://doi.org/10.51585/gjvr.2022.1.0038","url":null,"abstract":"Bovine brucellosis is an important zoonotic disease in Albania. Both B. abortus and B. melitensis have been isolated from ruminants. National control and eradication programs for brucellosis are applied on sheep and goat farms and larger dairy cattle farms. However, the current control programs for brucellosis do not cover small dairy cattle farms, and there is no valid data on the prevalence of the disease in this category; this prevents formulating evidence-based and effective strategies for control of the disease in this population subset. Therefore, the current study aimed to assess the herd and within-herd prevalence of bovine brucellosis in small bovine herds and provide scientific evidence for establishing an evidence-based approach to control the disease in this subset of the population not previously included in the national eradication program. To achieve this objective, a statistical survey was designed and implemented in small dairy herds in the Lushnja district, where samples from statistically selected herds were serologically tested in parallel with Rose Bengal Test (RBT), Fluorescence Polarization Assay (FPA) and Enzyme-Linked Immunosorbent Assay (ELISA). In total, 120 dairy herds were randomly selected from a list of 1,955 registered herds: from these selected herds, 368 blood samples were collected from all animals older than 12 months, and their sera were tested using RBT, FPA, and c-ELISA. The test results revealed no positive or suspect cases. Based on these results, we are confident (P0.05) that Brucella spp is not circulating in this subpopulation of cattle in the Lushja district. This deduction is supported by analyses of the main risk factors, other epidemiological data, and the perceptions of official and private veterinarians. This is the first structured survey of bovine brucellosis in small dairy herds in Albania. In conclusion, our study results and our findings show that the epidemiological status of bovine brucellosis in the Lushnja district is encouraging. Therefore, a test and slaughter control program appears appropriate in smaller herds. Furthermore, the approach used in this pilot study could be extended to establish the prevalence of brucellosis in other districts, the result of which would establish the basis for rational control measures in the smaller herds of cattle.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122342365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatocellular adenoma in a goat: An incidental abattoir finding in Oman","authors":"Haytham Ali, Abeer Alhamrashdi, M. El-Neweshy","doi":"10.51585/gjvr.2022.2.0030","DOIUrl":"https://doi.org/10.51585/gjvr.2022.2.0030","url":null,"abstract":"In the present study, a hepatic mass measured 9×11×5 cm in the right hepatic lobe was detected incidentally in a less than two years old male Somali goat during routine meat inspection at the Central Muscat Municipality Slaughterhouse, Oman. Gross and microscopic examination revealed a hepatocellular adenoma, a rare finding in goats.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115437771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus isolated from bovine raw milk in Njombe region, Tanzania","authors":"George J. Sanga, A. M. Lupindu, A. S. Hoza","doi":"10.51585/gjvr.2022.2.0029","DOIUrl":"https://doi.org/10.51585/gjvr.2022.2.0029","url":null,"abstract":"The emergence of methicillin-resistant Staphylococcus aureus (MRSA) creates a serious public health concern due to its ability to colonize and infect humans and animals. This cross-sectional study investigated the prevalence, antibiotic susceptibility pattern of S. aureus and MRSA isolated from bovine raw milk in the Njombe region, Tanzania. A total of 470 samples, including 389 raw milk samples collected at farm level, 57 raw milk samples from bulk milk-can at collection centers, and 24 swab samples from bulk milk cans. The samples were cultured on mannitol salt agar, presumptive colonies were sub-cultured onto blood agar for the isolation of S. aureus which was subsequently preliminarily confirmed using microbiological and biochemical tests. Further, confirmation of isolates was done using conventional PCR targeting gltB gene for S. aureus and mecA gene for MRSA which was later sequenced. Isolates were tested for antibiotic susceptibility by using the disc agar diffusion method. The overall prevalence of S. aureus in the study was 22.6% (106/470), with 2.9% (14/470) being MRSA. Both S. aureus and MRSA showed high resistance to penicillin (74%, 8.5%) and ampicillin (78%, 11.3%), respectively. A total of 81 (77%) isolates were resistant to at least one antibiotic and 14 isolates (13.2%) showed multidrug-resistant (MDR); with frequent antibiotic resistance patterns being to cefoxitin, penicillin, ciprofloxacin, tetracycline, and erythromycin. In conclusion, the prevalence and the MDR patterns exhibited by S. aureus and MRSA observed in this study provide baseline data for planning mitigation measures to safeguard public health.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122153277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of an outbreak of brucellosis in a mixed dairy farm and evaluation of a test and slaughter strategy to release the herd out of the quarantine","authors":"M. El-Diasty, Khaled Salah, Fatma El-hofy, Ashraf Abd El- Tawab, Enas A. Soliman","doi":"10.51585/gjvr.2022.1.0028","DOIUrl":"https://doi.org/10.51585/gjvr.2022.1.0028","url":null,"abstract":"An outbreak of brucellosis in a mixed dairy farm with 508 animals (370 cows, 120 sheep, and 18 camels) at Fayoum governorate, North Upper Egypt, was investigated. A storm of abortion and several cases of retained placenta were reported among cows and ewes in April 2020. Serodiagnosis of brucellosis was done using Rose Bengal Test (RBT) and Buffered Acidified Plate Antigen Test (BAPAT). The Milk Ring Test (MRT) was applied to the milk of seropositive animals. A total of 89 samples were used for isolation of Brucella and isolates were confirmed using Abortus, Melitensis, Ovis, Suis-PCR (AMOS-PCR). Test and slaughter strategy was applied to eradicate brucellosis from the farm based on RBT every month until three successive negative tests were obtained. Results showed that the seroprevalences of brucellosis based on RBT and BAPAT were 9.5%, 35%, and 50% in cattle, sheep, and camels, respectively. Despite 50% of male camels being seropositive, no clinical signs have been reported. The MRT identified fewer positive cases than BAPAT and RBT, thus, it cannot be used alone to eliminate the infection from the farm. A total of 31 Brucella isolates were recovered from cows and sheep on the farm. All isolates were confirmed as Brucella melitensis bv3 based on bacteriological examination and Brucella AMOS-PCR confirmed all isolates as Brucella melitensis. No positive reactors at the 6th, 7th, 8th, 9th, and 10th examinations were reported after the implementation of the test and slaughter strategy. In conclusion, extensive animal farming and mixed breeding are potential risk factors for interspecies transmission of brucellosis. Additionally, the test and slaughter strategy could be helpful to release the herd out of quarantine, however, application of biosecurity practices and fair compensation policy for owners should be implemented.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"449 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123432359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equine synovial fluid protein equalization via combinatorial peptide ligand libraries","authors":"P. Fueyo, M. Galleguillos, Cristóbal A. Dörner, Pedro A. Smith, Francisca Godoy, H. Adarmes","doi":"10.51585/gjvr.2021.4.0026","DOIUrl":"https://doi.org/10.51585/gjvr.2021.4.0026","url":null,"abstract":"To gain further knowledge of the equine synovial fluid (SF) proteome, we propose a protocol based on the equalization of the relative concentrations of its proteins, which leads to the modification of the standard electrophoretic pattern revealing low-abundance proteins that otherwise remain undetected. Fresh SF samples were collected from ten macroscopically normal metacarpophalangeal joints of crossbred horses. The samples were processed using standard procedures as the control and via combinatorial peptide ligand libraries (CPLL) using low ionic forces (NaH2PO4 10 mM) at different pHs (4.0, 7.0, and 9.3) with 10% sodium dodecyl sulfate (SDS) and 25 mM DTT for protein resolubilization. Proteins were then separated by conventional 8% SDS-PAGE and stained with coomassie blue. After separation of the equalized proteins, there was a significant reduction in the albumin (the most abundant protein in the SF) and, at the same time, other protein bands arise that were not visible without CPLL processing. In addition, there was variation in the protein profiles at different pHs. The results suggest that protein equalization of the equine SF by CPLL could be a useful tool to better understand the articular homeostasis and/or for the detection of new biomarkers of joint pathology.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124280402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a direct-fed microbial (PrimaLac) and fat inclusion on performance and energy metabolism in broiler chicks and turkey poults","authors":"Samantha Black, A. Fahrenholz, J. Grimes","doi":"10.51585/gjvr.2021.4.0024","DOIUrl":"https://doi.org/10.51585/gjvr.2021.4.0024","url":null,"abstract":"Two battery trials were conducted to determine the effects of dietary direct-fed microbial (DFM) and dietary fat inclusion on broiler chick and turkey poult performance and dietary apparent metabolizable energy, nitrogen corrected (AMEn) when fed corn, wheat, and soy diets. For both experiments, a 2 x 2 factorial experimental design was used with supplemental fat at low (1%, LF) or high (6%, HF) levels and DFM inclusion (0 or 0.91 kg/ton) as main effects. Dietary treatments were randomly assigned to 72 cages of birds and fed for 21 days. Growth performance was measured weekly, and cecal contents were collected for volatile fatty acid (VFA) analysis on day 21. Fat supplementation resulted in increased body weight gain (BWG) in both broilers and turkeys. Specifically, in LF diets, DFM inclusion resulted in increased BWG compared to the non-DFM treatments. Cumulative feed conversion ratio (FCR) was improved in HF treatments and the LF with DFM treatment compared to the LF with no DFM. In both trials, as expected, feeding HF diets resulted in increased AMEn. However, the DFM inclusion resulted in a greater uplift in AMEn in LF diets for both broilers and turkeys. In either trial, diet did not impact cecal VFA concentrations; thus, the impact on DFM on VFA production remains uncertain. In conclusion, the performance of broiler chicks and turkey poults was improved by DFM inclusion in reduced fat diets which was associated with increased energy digestibility as measured by AMEn.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130323539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}