German Journal of Veterinary Research最新文献

{"title":"Evaluation of the antinociceptive effect of lidocaine- tramadol and lidocaine- medetomidine lumbosacral epidural anesthesia: A cross-over comparative study in goats","authors":"Fatimah T. Al-Ali, A. Almubarak, M. Marzok, Wael M. El-Deeb, S. F. El-Hawari","doi":"10.51585/gjvr.2023.4.0066","DOIUrl":"https://doi.org/10.51585/gjvr.2023.4.0066","url":null,"abstract":"Small ruminants have a docile temperament and are typically operated under local or regional analgesia. In goats, lumbosacral anesthesia is the most commonly used regional anesthesia. This investigation aimed to evaluate the epidural anesthetic effects of lidocaine-medetomidine (LID-MED) and lidocaine-tramadol (LID-TRM) combinations in relation to cardiopulmonary effects. An experiment using a cross-over design was conducted on eight goats. The first group (LID-MED) was injected with lidocaine hydrochloride and medetomidine hydrochloride. The second group (LID-TRM) was injected with lidocaine hydrochloride and tramadol hydrochloride. The onset of analgesia, recumbency time, and standing time were recorded once, while scores were recorded periodically. Locomotor and anti-nociception scores were evaluated at baseline, 5, 10, 15, 30, 60, 90, and 120 minutes (min) post-anesthesia. Similarly, cardiorespiratory values were also recorded at the same intervals in each group. In the LID-MED receiving group, analgesia and recumbency onset were earlier, with a longer recumbency period. The LID-MED group showed a significant loss of sensation in all examined regions. The locomotor score revealed hind limb paralysis for 90 min in the LID-TRM group, while it continued for 120 min in the LID-MED group. In both LID-TRM and LID-MED groups, there was significant hypothermia; however, bradycardia was noticed in the LID-MED group from 5 min post-injection. Respiratory depression was also detected in the LID-MED group. The study revealed that lumbosacral epidural anesthesia using LID-TRM co-infusion produces reasonable and short duration (60 min) analgesia. In contrast, epidural lumbosacral injection of LID-MED co-infusion produces a longer duration of analgesia and recumbency.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"51 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139192630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on equine colic: Etiology, differential diagnosis, therapy, and prevention","authors":"Ragab M. Fereig","doi":"10.51585/gjvr.2023.4.0063","DOIUrl":"https://doi.org/10.51585/gjvr.2023.4.0063","url":null,"abstract":"Equines encompass various animals, such as horses, donkeys, ponies, mules, and zebra. These animals are means of transportation, leisure, source of meat, and income worldwide. Equine colic is among the equines' most important, common, and emergent health issues, particularly in horses. The complexity and anatomical characteristics rendered equines prone to equine colic. Different classifications for equine colic are known depending on cause, affected site, and duration. In the current study, we have exhaustively focused on etiological, clinical findings, clinical and laboratory diagnosis, and eventually, the treatment and control of equine colic. Such knowledge is lacking, and some reports discuss only one or few aspects. Because they are the most prevalent cause, we have focused on gastrointestinal disorders as a cause of colic. Moreover, recent approaches for diagnosis and prognosis were investigated mainly via the biomarkers analysis assay. Because of its importance, similarity to other equines, worldwide distribution, and plenty of available research, we mostly specified our description of horses as a model of equine animals. This review offers valuable insights for veterinarians and equine practitioners, potentially aiding in mitigating health risks and fatal consequences associated with colic in equines","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139300332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avian influenza and the Brazilian poultry production: Current situation and prevention strategies","authors":"Ricardo H. Rauber","doi":"10.51585/gjvr.2023.4.0064","DOIUrl":"https://doi.org/10.51585/gjvr.2023.4.0064","url":null,"abstract":"Highly Pathogenic Avian Influenza (HPAI) presents a significant threat to global poultry, affecting trade and consumer trust. This analysis focuses on Brazil's preventive measures against HPAI to uphold its disease-free status recognized by the World Organization for Animal Health (WOAH). As of September 19th, 2023, Brazil confirmed 106 HPAI outbreaks, primarily in wild birds, with a few in backyard chickens. Despite most incidents occurring along the coast, a notable outbreak in Bonito-MS underlines the necessity for ongoing vigilance in key poultry regions. Brazil's HPAI prevention framework, initiated with the 1994 National Poultry Health Plan, has continually evolved. High biosecurity measures are emphasized in stringent regulations, surveillance, and rapid response strategies. The nation's vertically integrated poultry model supports centralized control, traceability, and consistent quality, fostering swift corrective actions and biosecurity guidelines. The private sector's commitment complements these efforts, often exceeding government regulations with rigorous biosecurity practices, highlighting Brazil's holistic approach to safeguarding its poultry sector from HPAI threats.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139302834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of bacterial pathogens isolated from smoked blue whiting fish (Micromesistius poutasou) from Odeomu market in Osun state Nigeria","authors":"O. Aladejana, M. Zakka, A. Ogunlade, O. Thonda","doi":"10.51585/gjvr.2023.2.0055","DOIUrl":"https://doi.org/10.51585/gjvr.2023.2.0055","url":null,"abstract":"The presence of microorganisms is one of the major factors affecting the quality of smoked fish sold in the open markets. Smoked blue whiting fish (Micromesistius poutasou), commonly called (Panla) sold in the Odeomu market in Osun State, were analyzed for microbial contaminants. Isolates were identified using conventional biochemical methods, and antibiotics susceptibility testing was carried out using the disc diffusion method. The total bacterial counts (TBC) results showed that the fish samples had high bacterial counts, ranging from 2.1×103 to 9.2×103 colony-forming units (CFU)/g. Bacteria isolated from the fish samples were: E. coli (45.46%), Enterobacter spp. (1.01%), Klebsiella spp. (6.06%), Proteus spp. (9.09%), Salmonella spp. (7.07%), Shigella spp. (19.19%), Bacillus spp. (4.04%) and Staphylococcus spp. (8.08%). The antibiotic sensitivity pattern of Gram-Negative bacteria indicated that all the isolates were resistant to more than three antibiotics. All E. coli isolates were resistant to augmentin and ceftazidime, 82.2% were resistant to cefuroxime, 17.7% to gentamicin, and 6.7% to ofloxacin. Screening of resistance genes showed that all six selected multiple antibiotic-resistant E. coli isolates tested harbored TEM gene, and two isolates (33.33%) harbored the aac (3)-II gene. None of the isolates harbored SHV, CTX-M, and qnr B genes. Our results showed that smoked blue whiting fish may pose a significant risk of spreading antibiotic-resistant bacteria that contain multiple antibiotic-resistance genes, highlighting a serious public health concern","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122944914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of antigen-capture ELISA using monoclonal antibodies for the detection of brucellae in milk","authors":"Sevil E. G¨urbilek, O. Keskin, O. Y. Tel","doi":"10.51585/gjvr.2023.3.0056","DOIUrl":"https://doi.org/10.51585/gjvr.2023.3.0056","url":null,"abstract":"In this study, a Brucella antigen-capture ELISA (Ag-cELISA) prototype was developed. To study the validity of the developed Ag-cELISA, milk samples collected from Brucella-positive goats (n=120) and cattle (n= 64), as well as from unknown Brucella-status cattle (n=105) and sheep (n=65) herds were tested by Ag-cELISA, I-ELISA, and culture method. All Brucella-positive samples were confirmed using PCR. It was found that the developed Ag-cELISA could detect 50-100 bacteria per well (equivalent to 103 to 2×103 cells per mL) as the lowest limit of detection (LOD) and was therefore considered moderately sensitive to detect brucellae in milk. In an infected goat herd, out of 120 milk samples, 41, 32, and 17 were positive by Ag-cELISA, I-ELISA, and culture, respectively. Ag-cELISA detected 15 positive cases out of 17 culture-positive milk samples. Two culture-positive milk samples were not detected in Ag-cELISA. The relative sensitivity and specificity between Ag-cELISA and I-ELISA were 78% and 100%, respectively. In an infected cow herd, out of 64 milk samples, 32, 23, and 11 were found positive by Ag-cELISA, I-ELISA, and culture, respectively. Ten out of 11 culturally positive milk samples were found positive by Ag-cELISA. The relative sensitivity and specificity between the Ag-cELISA and I-ELISA were 71.9% and 100%, respectively. From randomly collected 105 cow and 110 sheep milk samples from herds of unknown Brucella-infection status, three (2.85%) and five (4.5%) samples were found positive using Ag-cELISA, respectively. These results showed that Ag-cELISA could be used to detect brucellae in milk more practically and safely than bacterial culture. On the other hand, this information re-affirms that milk can be an important source of brucellosis and creates a public health risk in humans; therefore, increased public awareness is of utmost importance.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125039627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of dietary spray-dried bovine plasma fed to turkeys during brooding on performance to market age","authors":"A. A. Gernat, J. Campbell, A. Fahrenholz, J. Grimes","doi":"10.51585/gjvr.2023.2.0054","DOIUrl":"https://doi.org/10.51585/gjvr.2023.2.0054","url":null,"abstract":"Commercial turkey production can experience an economic loss due to exposure of birds to stress. Turkeys can undergo various levels of stress, including, but not limited to, hatching, brooding/growing, and transport. Temperature, climate, heat, and cold stress are major livestock stressors associated with economic losses. Exposure to stress also causes an increased probability of infections and diseases that have a negative financial impact on production. In this study, Large White commercial turkey hens were reared for 12 weeks (wk) to evaluate their stress responses and performance alterations due to induced stress through a mimic of brooder house to grow-out transition. Parameters for performance, blood, and meat yield were recorded. Spray-dried bovine plasma (SDBP) was formulated iso-nutritionally into the diets. SDBP has been shown to be an ingredient in animal diets that may help support immune health and positively affect performance. This ingredient was used for a total of 6 wk in the starter and grower-1 diets at different inclusion percentages. Treatments included a control diet (0% SDBP), 1.0% (SDBP1), and 2.0% (SDBP2) inclusion. At 6 wk, common diets were fed (grower-2 and finisher-1). At 5 wk management-based stressors were applied for 24 hours (h): feed and water restriction and reduced house temperature. Previously used pine shavings were used for bedding. No significant variances were noted in body weight, weight gain, or feed consumption as a result of the different feed treatments. However, during the sixth week, the feed conversion ratio (FCR) was improved due to SDBP inclusion. The FCR for SDBP1 (1.90) and SDBP2 (1.97) birds was lower than that of the control diet-fed birds (2.04). No difference in FCR was observed at 12 wk. A significant difference was observed for corticosterone levels post-stress: SDBP1 (23.81 ng/mL) and SDBP2 (19.17 ng/mL) were higher than that of the control birds (16.40 ng/mL). Further research is needed to ascertain the impact of SDBP on the immune function and production of turkeys.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132926254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first report of Brucella melitensis biovar 2 strain isolated from cattle in Turkey","authors":"Kadir Akar, Gülseren Yıldız Öz","doi":"10.51585/gjvr.2023.2.0053","DOIUrl":"https://doi.org/10.51585/gjvr.2023.2.0053","url":null,"abstract":"Bovine brucellosis is an infectious zoonotic disease of great impact on animal welfare and has significant economic implications on livestock farm worldwide. The disease is caused primarily by Brucella abortus (B. abortus), while B. melitensis is less common, and B. suis infection is rare. B. melitensis is the most common causative agent of brucellosis in small ruminants and humans. Although the main host of B. melitensis is considered to be small ruminants, this bacterium is also present in large ruminants. Despite brucellosis has been eradicated in many European countries, it is still endemic in Mediterranean countries and Turkey. The most prevalent Brucella species in the Mediterranean basin and Turkey is B. melitensis biovar (bv) 3. Previous studies have reported that B. melitensis bv2 is quite low in Turkey. This is the first study to isolate B. melitensis bv2 from cattle in Turkey. The strains were characterized using classical biotyping methods and then were molecularly confirmed. Multilocus variable number tandem repeat analysis (MLVA-16) typing of the strains revealed a novel genotype (1-5-3-13-3-2-3-2-4-41-8-5-4-3-3-7), which matches the Multilocus sequence typing (MLST) profiles in the database of ST8 (3-2-3-2-1-5-3-2-8). These results indicate that B. melitensis bv2 can easily infect cattle and this has to be considered in the epidemiology and control of bovine brucellosis. Circulating the highly pathogenic B. melitensis bv2 in cattle farms is of public health concern.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127257516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chronic stress and intestinal inflammation on commercial poultry health and performance: A review","authors":"G. Téllez-Isaías, W. Eisenreich, V. Petrone-García, X. Hernández-Velasco, Castellanos-Huerta Castellanos-Huerta, Guillermo Tellez Jr, J. Latorre, W. Bottje, R. Señas-Cuesta, M. Coles, B. Hargis, S. El-Ashram, B. Graham, A. Shehata","doi":"10.51585/gjvr.2023.1.0051/","DOIUrl":"https://doi.org/10.51585/gjvr.2023.1.0051/","url":null,"abstract":"The gastrointestinal tract provides the biological environment for nutrient digestion and absorption. Its physical and chemical barriers are crucial to protect from invading pathogens and toxic substances. On this basis, the intactness of the gastrointestinal tract, with its multiple functions and impacts, is one of the key prerequisites for human and animal health. Undoubtedly, the functions of a healthy gut system also largely benefit the welfare and performance of animals in farming systems such as poultry industries. Broiler chickens grow rapidly, as a result of rigorous genetic programs, due to the high absorption capacity of intestinal epithelia for nutrients, the quick transport of nutrients to the muscle, and their efficient conversion into energy and biomass. Due to oxygen metabolism or enteric commensal bacteria, intestinal epithelial cells create reactive oxygen and nitrogen species physiologically. However, increased generation of these oxidants goes along with the formation of free radicals resulting in oxidative stress causing lipid peroxidation and dramatic molecular changes in the structure and function of the cell and mitochondrial membranes. These effects contribute to chronic oxidative stress and inflammation of the gastrointestinal tract and generally affect all chicken organs, tissues, and cells. Hence, all forms of chronic stress, regardless of the origin, negatively impact the chicken's overall performance, health, and welfare. This review article highlights some enteric inflammation models and biomarkers to evaluate gut integrity in chickens and discusses the repercussions that chronic stress and intestinal inflammation have on the health and performance of commercial poultry","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129206040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic comparison of Brucella spp. and Ochrobactrum spp. erroneously included into the genus Brucella confirms separate genera","authors":"Katharina Holzer, L. Hoelzle, G. Wareth","doi":"10.51585/gjvr.2023.1.0050","DOIUrl":"https://doi.org/10.51585/gjvr.2023.1.0050","url":null,"abstract":"The facultative intracellular pathogen Brucella and the free-living bacteria Ochrobactrum are both α-proteobacteria and very close to each other. A group of researchers recently clustered Ochrobactrum strains into the genus Brucella according to a BLAST distance approach. Thus, we performed a deeper comparative genetic analysis for eleven Ochrobactrum strains and twelve different Brucella isolates to demonstrate important differences between these bacteria. In addition to the clear differences between Brucella and Ochrobactrum, like the differences in genes contents, and different genome sizes, the Brucella-specific gene bscp31 was not found in Ochrobactrum, as well as other important Brucella-specific proteins and virulence factors. Differences in antimicrobial resistance genes content and the presence or absence of plasmids were obvious between Brucella and Ochrobactrum spp. Genome alignment of Brucella spp. and Ochrobactrum spp. revealed a genome similarity of 85.7% maximum, whereas all analyzed Brucella spp. in this study had a similarity of 97.6-99.9%, and all compared Ochrobactrum spp. 82.6-98.0%. Because of these facts mentioned in this work, Brucella and Ochrobactrum should be considered separate genera.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121892795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance of Salmonella strains isolated from food products of animal origin in Ukraine between 2018-2021","authors":"T. Kozytska, O. Chechet, T. Garkavenko, V. Nedosekov, O. Haidei, O. Gorbatiuk, V. Andriyashchuk, Viacheslav Kovalenko, D. Ordynska, Natalia Kyriata","doi":"10.51585/gjvr.2023.1.0049","DOIUrl":"https://doi.org/10.51585/gjvr.2023.1.0049","url":null,"abstract":"Salmonellosis is a zoonotic infection of humans and animals. In recent years, an increase in the number of resistant strains of Salmonella from animals and animal products have been recorded. We conducted microbiological testing and antibiotic resistance profile of 34 Salmonella strains isolated from foods of animal origin between 2018 to 2021 in Ukraine. Twenty isolates were successfully assigned to six different serovars [S. Enteritidis (n=13, S. Infantis (n=2), S. Schwarzengrund (n=1), S. Livingstone (n=2), S. Vuadens (n=1) and S. Hadar (n=1)], and 14 isolates remained untyped. Most of the isolated strains were recovered from poultry products. The strains were tested for six antibacterial groups: quinolones, aminoglycosides, β-lactams, cephalosporins, phenols, and dihydrofolate reductase inhibitors by disc diffusion method. The highest level of resistance was recorded to 4th generation cephalosporins, e.g., cefepime (88.2%). The lowest level of resistance was to cefotaxime and ceftriaxone (5.9%). No resistance was found to imipenem and aminoglycoside antibiotics, particularly gentamicin and amikacin. The study results are of concern because antibiotic-resistant bacteria and their genes can cause human infections by penetration and transmission at any stage of the food production cycle. Further research is required to monitor the contamination of food products of animal origin with salmonellosis pathogens, including antibiotic-resistant strains.","PeriodicalId":310978,"journal":{"name":"German Journal of Veterinary Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125066527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}