Jurnal Agripet最新文献

{"title":"Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas","authors":"Bagus Dwijayanti, Erdiansyah Rahmi, Ummu Balqis, F. Fitriani, D. Masyitha, D. Aliza, Muslim Akmal","doi":"10.17969/AGRIPET.V21I2.16012","DOIUrl":"https://doi.org/10.17969/AGRIPET.V21I2.16012","url":null,"abstract":"ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P˂0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P˂0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7.  (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P˂0.05) on macroscopic morphometry of the native chicken’s intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smo","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43346006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologi, Histomorfometri, dan Histokimia Hati Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas","authors":"Siswandy Siswandy, Erdiansyah Rahmi, D. Masyitha, F. Fitriani, Fadli A. Gani, Zuhrawaty Zuhrawaty, Muslim Akmal","doi":"10.17969/agripet.v20i2.16011","DOIUrl":"https://doi.org/10.17969/agripet.v20i2.16011","url":null,"abstract":"ABSTRAK. Hati adalah kelenjar terbesar yang terdapat di dalam tubuh dan dapat dianggap sebagai organ pusat dalam pemeliharaan suplai energi. Penelitian ini bertujuan untuk mengetahui histologi, histomorfometri, dan histokimia sebaran glikogen pada hati ayam buras sebelum dan sesudah menetas. Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam hati ayam. Hasil penelitian menunjukkan perbedaan nyata (P0,05) pertumbuhan hati ayam buras secara makroskopis. Pada setiap perkembangan sudah terdapat hepatosit berbentuk poligon yang memiliki inti sel bulat di tengah hepatosit, terdapat juga vena central yang dilapisi sel endotel pipih selapis serta didapati sinusoid yang berada diantara hepatosit, dan juga ditemukan Kupffer’s cell hanya pada hati ayam setelah menetas hari ke-7. Hasil pengukuran diameter vena central hati ayam buras hari ke-7, hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 17,70 ± 1,16 µm; 60,32 ± 2,22 µm; 49,316 ± 1,93 µm; dan 129,11 ± 7,43 µm. Hasil histokimia sebaran glikogen pada hati ayam buras hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 1,44 ± 0,93; 2,27 ± 0,74; dan 2,77 ± 0,15. Dapat disimpulkan, bahwa diameter vena central dan sebaran glikogen meningkat seiring pertumbuhan ayam buras. (Histological, histomorphometrical, and histochemical of liver on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRACT. The liver is the largest gland in the body and considered as a central organ in maintaining energy supplies. The study aimed to determine the histologic, histomorphometric and histochemical of glycogen distribution in the liver of native chicken during pre and posthatching period.. The study sample were divided into four different age groups and each group consisted of six chicken livers. The results showed a significant difference (P0,05) in native chicken liver growth macroscopically. In every development there are already polygonal hepatocytes that have a round cell nucleus in the middle of the hepatocytes, there is also a central vein that is coated with a layer of flat endothelial cells and also sinusoids that are between the hepatocytes, and kupffer's cells also found in chicken liver 7th day posthatching. The results measurements of the central vein diameter in liver native chickens on the 7th, 14th, 20th pre hatching period and 7th post hatching were 17.70 ± 1.16 µm; 60.32 ± 2.22 µm; 49.32 ± 1.93 µm; and 129.11 ± 7,43 µm; The measure results of the glycogen distribution in liver native chickens on the 14th, 20th pre hatching period and 7th post hatching were 1,44 ± 0,93; 2,27 ± 0,74; and 2,77 ± 0,15. It can be concluded, that central vein diameter and glycogen distribution were increased by growth of native chickens.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41439692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolasi dan Identifikasi Bakteri Gram Negatif pada Uterus Sapi Aceh yang Mengalami Repeat Breeding","authors":"Indi Rafika, Cut Nila Thasmi, Herrialfian Herrialfian, Rosmaidar Rosmaidar, Hafizuddin Hafizuddin","doi":"10.17969/AGRIPET.V20I2.17017","DOIUrl":"https://doi.org/10.17969/AGRIPET.V20I2.17017","url":null,"abstract":"ABSTRAK. Penelitian ini bertujuan mengetahui jenis bakteri Gram negatif pada uterus sapi Aceh yang mengalami kawin berulang. Dalam penelitian ini digunakan enam ekor sapi Aceh betina yang terdiri atas tiga ekor sapi fertil (K1) dan tiga ekor yang mengalami kawin berulang (K2, repeat breeding, RB). Pengambilan sampel bakteri dalam uterus sapi dilakukan dengan menggunakan swab steril yang disimpan dalam media nutrient broth (NB) untuk isolasi dan identifikasi jenis bakterinya. Berdasarkan hasil penelitian, baik K1 dan K2, positif terinfeksi bakteri dan diperoleh 16 isolat bakteri di antaranya enam isolat berasal dari K1 dan sepuluh isolat berasal dari K2. Dari 10 isolat bakteri Gram negatif yang ditemukan pada sapi RB terdiri atas Escherichia coli (E. coli) (30,00%), Pseudomonas sp. (30,00%), Enterobacter sp. (20,00%), Klebsiella sp. (20,00%). Disimpulkan bahwa bakteri yang paling banyak menginfeksi uterus sapi RB adalah E. coli dan Pseudomonas sp. (Isolation and identification of gram negative bacteria in uterine Aceh cow with repeat breeding) ABSTRACT. The study determined the type of Gram negative bacteria of the Aceh cattle uterus which has been diagnosed with repeat breeding. The study was conducted on six Aceh cows consists of 3 normal fertile cows (K1) and 3 repeat breeding cows (K2, RB). The bacteria in uterine samples were collected using sterile swab, kept into nutrient broth (NB) for further isolation and identification of bacteria. The result of the study showed that the total of 16 isolates of bacteria was observed in all cows : 6 isolates from group K1 and 10 isolates from group K2. A total of 10 isolates from cattle with repeat breeding consist of Escherichia coli (E. coli) (30.00%), Pseudomonas sp (30.00%), Enterobacter sp (20.0%), and Klebsiella sp (20.0%). It can be concluded that the most bacteria that infect uterine cows with repeat breeding are E. coli and Pseudomonas sp.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49060844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efektivitas Pemberian Beberapa Preparat Hormon Prostaglandin Komersial terhadap Persentase Berahi Sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara","authors":"Novika Ayuni Rambe, T. N. Siregar, T. A. Tr, G. Gholib, B. Panjaitan, Mulyadi Adam, D. Dasrul","doi":"10.17969/AGRIPET.V20I2.16317","DOIUrl":"https://doi.org/10.17969/AGRIPET.V20I2.16317","url":null,"abstract":"ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2α analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2α-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2α-1 mempunyai efektivitas lebih baik dibandingkan PGF2α-2 dan PGF2α-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara.  (Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera) ABSTRACT. The aim of this study was to determine the effect of differences PGF2α analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2α-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2α-3 (cloprostenol 75 µg/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45997157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Pemberian Tumbuhan Obat terhadap Performa Produksi dan Karakteristik Reproduksi Ayam Petelur","authors":"Nurmeiliasari Nurmeiliasari, Y. Fenita, Ahmat Kusnandar","doi":"10.17969/agripet.v20i1.15269","DOIUrl":"https://doi.org/10.17969/agripet.v20i1.15269","url":null,"abstract":"ABSTRAK. Performa produksi, jumlah folikel dan berat organ reproduksi diukur pada 40 ekor ayam petelur yang diberi perlakuan penambahan 5% tepung daun tumbuhan obat yaitu daun Katuk (5%TDK), daun Marigold (5%TDM), daun Senduduk (5%TDS) ke dalam ransum. Variabel yang diamati meliputi konsumsi ransum, persentase produksi telur, egg mass production, konversi ransum, berat dan panjang organ reproduksi dan jumlah folikel. Penambahan tepung tanaman obat tidak mempengaruhi konsumsi ransum ayam petelur. Hasil perhitungan persentase produksi telur mingguan menunjukkan hasil yang sama pada semua perlakuan. Perlakuan 5% tepung tanaman herbal tidak mempengaruhi egg mass production. Konversi ransum pada semua kelompok perlakuan tanaman obat adalah sama. Pemberian tepung tanaman obat di dalam ransum petelur tidak berpengaruh nyata pada jumlah folikel. Berat dan panjang organ reproduksi tidak dipengaruhi oleh perlakuan tepung tanaman obat. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan 5% tepung daun Katuk, Marigold dan Senduduk dalam ransum menunjukkan konsumsi ransum, persentase produksi telur, egg mass production, konversi ransum, bobot dan panjang oviduk, berat ovarium dan jumlah folikel yang sama pada ayam petelur.         (Efficacy of medicinal plants on production performance and reproductive characteristics of laying hens) ABSTRACT. Efficacy of Katuk leaf meal, Marigold leaf meal and Senduduk leaf meal on production performance and reproductive characteristics were evaluated. Forty layers aged 32 weeks were distributed into 4 dietary treatments. The layers were housed in individual battery. One-way ANOVA treatment arranged in a Completely Randomized Design was used in this experiment. Dietary treatments were P0 (control without medicinal leaf meal), P1 (diet with 5% Katuk leaf meal), P2 (diet with 5% Marigold leaf meal), P3 (diet with 5% Senduduk leaf meal). This research measured feed intake, egg production, percentage of egg production, feed conversion ratio, number of follicles, weight of ovary, weight and length of oviduct as parameters. Significant results were tested by Duncan Multiple Range Test. The result showed that dietary treatments of medicinal leaf did not affect feed intake and feed conversion ratio of layers. Egg production percentage and egg mass production, the number of follicles, and length and weight of oviduct were similar of all medicinal leaf meal treatments. It can be concluded that feeding medicinal leaf meal did not affect production performance, number of follicles and weight of reproductive organs.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41965490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kecernaan In-Vitro Fraksi Serat Kombinasi Pucuk Tebu dan Titonia Fermentasi sebagai Pakan Ruminansia","authors":"D. Susanti, N. Jamarun, F. Agustin, T. Astuti, G. Yanti","doi":"10.17969/agripet.v20i1.16040","DOIUrl":"https://doi.org/10.17969/agripet.v20i1.16040","url":null,"abstract":"ABSTRAK. Pucuk tebu dan titonia merupakan hijauan alternatif yang dapat digunakan sebagai sumber energi dan sumber protein pada ternak ruminansia, tetapi terkendala dengan adanya kandungan lignin dan zat anti nutrisi. Untuk itu dilakukan penelitian yang bertujuan untuk mengetahui pengaruh kombinasi pucuk tebu dan titonia fermentasi terhadap kecernaan fraksi serat (NDF, ADF, selulosa dan hemiselulosa) secara in-vitro. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 4 perlakuan yaitu A (100% Pucuk Tebu Fermentasi + 0% Titonia Fermentasi), B (75% Pucuk Tebu Fermentasi + 25% Titonia Fermentasi), C (50% Pucuk Tebu Fermentasi + 50% Titonia Fermentasi), dan D (25% Pucuk Tebu Fermentasi + 75% Titonia Fermentasi) dengan 5 ulangan. Pengaruh perlakuan terhadap parameter yang diuji dianalisis menggunakan sidik ragam dan dilanjutkan dengan uji jarak berganda Duncan jika ada perbedaan antar perlakuan. Hasil sidik ragam menunjukkan bahwa perlakuan memberikan pengaruh yang berbeda sangat nyata (P0,01) terhadap kecernaan fraksi serat (NDF, ADF, selulosa dan hemiselulosa). Dari hasil penelitian dapat disimpulkan bahwa perlakuan C (50% Pucuk Tebu Fermentasi + 50% titonia Fermentasi) memberikan hasil yang terbaik terhadap kecernaan NDF (78,13%), ADF (76,76%), selulosa (80,81%) dan hemiselulosa (81,11 %).  (In-vitro digestibility of fiber fractions combination of sugarcane tops and tithonia as ruminant feed) ABSTRACT. Sugarcane tops and tithonia are alternative forages that can be used as an energy source and protein source in ruminants, but are constrained by the presence of lignin and anti nutrients. For this reason, a study was conducted to determine the effect of a combination of fermented sugarcane tops and fermented tithonia on the digestion of the fiber fraction(NDF, ADF, cellulose, and hemicellulose).The research design used a randomized block design (RBD) with 4 treatments, namely A (100% fermented sugarcane tops and 0% fermented tithonia), B (75% fermented sugarcane tops and 25% fermented tithonia), C (50% fermented sugarcane tops and 50% fermented tithonia), D (25% fermented sugarcane tops and 75% fermented tithonia) with 5 replications. Effect of treatment on parameters tested using analysis of variance and continued with Duncan's Multiple Range Test (DMRT) if there are differences between treatments. The results of experiments showed that treatments gave a very significant difference (P 0.01) to the in-vitro digestibility of fiber fraction (NDF, ADF, cellulose, and hemicellulose). From the results of the study, it can be concluded that the treatment C (50% sugarcane tops fermentation and 50% fermented tithonia) gives the best results on the in-vitro digestibility of fiber fraction NDF (78,13%), ADF (76,76%), cellulose (80,81%) and hemicellulose (81,11 %).","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42476112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Perbedaan Suhu Pemanasan terhadap Aktivitas Antibakteri Susu Kambing Fermentasi Menggunakan Lactobacillus Plantarum","authors":"Zuraida Hanum, Yurliasni Yurliasni, Sitti Wajizah, J. Juanda","doi":"10.17969/agripet.v19i2.14330","DOIUrl":"https://doi.org/10.17969/agripet.v19i2.14330","url":null,"abstract":"ABSTRAK. Penelitian ini bertujuan untuk melihat pengaruh suhu pemanasan yang berbeda terhadap aktivitas antibakteri susu kambing fermentasi menggunakan starter Lactobacillus plantarum. Bakteri uji yang digunakan berasal dari golongan Enterobacteriaceae yaitu Escherichia coli dan Salmonella.sp. Rancangan penelitian yang digunakan adalah RAL (Rancangan Acak Lengkap) Faktorial yang terdiri dari 2 faktor yaitu faktor A adalah suhu pemanasan, yaitu a1 (suhu pemanasan 72°C), a2 (suhu pemanasan 121°C) dan faktor B adalah level penambahan Lactobacillus plantarum yaitu perlakuan b1 (Lactobacillus plantarum 2,5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7,5%), masing-masing perlakuan diulang sebanyak 3 kali sehingga diperoleh 18 unit percobaan. Apabila terdapat perbedaan yang nyata maka dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan adanya interaksi dari suhu pemanasan yang berbeda dan penambahan L. plantarum pada susu kambing berpengaruh nyata terhadap kadar asam laktat dan daya hambat pada Salmonella sp, akan tetapi tidak berpengaruh nyata terhadap E. coli. Kesimpulan dari penelitian ini diperoleh suhu pasteurisasi dengan penambahan 7.5 % L.plantarum merupakan nilai terbaik dalam menghambat Salmonella dan E..coli.  (Effect of different heating temperature on antibacterial activity of goat milk fermented with Lactobacillus Plantarum) ABSTRACT. This study aimed to determine the effect of the different heating temperatures on inhibitory activity of fermented goat's milk with Lactobacillus plantarum. The test bacteria used were Enterobacteriaceae, namely Escherichia coli and Salmonella.sp. The design applied is CFD (Completely Randomized Design) consisting of 2 factors, namely factor A heating temperature with two levels a1 (72°C), a2 (121°C) and factor B is the level of Lactobacillus plantarum addition by three levels, namely b1 (Lactobacillus plantarum 2.5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7.5%), and the treatments were repeated 3 times. The results showed that different heating temperature and level of L. plantarum added in fermented goat milk significantly affected the levels of lactic acid and inhibitory power on Salmonella sp, but not on E. coli. In conclusion is different heating temperatures and addition of L. plantarum on goat milk at significantly affected on inhibitory power on Salmonella sp, but not on E. coli. The results obtained pasteurisasi with 7.5% L.plantarum could be applied.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43411232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Lon-like Protease Gene from Lactobacillus plantarum IIA-1A5 in Escherichia coli BL21(DE3)","authors":"O. Mega, C. Sumantri, I. I. Arief, C. Budiman","doi":"10.17969/agripet.v19i2.14904","DOIUrl":"https://doi.org/10.17969/agripet.v19i2.14904","url":null,"abstract":"Proteases are one of most important and abundant enzymes produced by the biotechnology industry, for scientific, physiological and industrial application and dominates of the whole enzyme market. Lactobacillus plantarum IIA-1A5 is an Indonesian lactic acid bacteria (LAB) isolated from beef Peranakan Ongole cattle. Preliminary analysis on its whole genome sequence indicated that this strain harbours some genes involved in protein degradation and might be promising to be further applied. This study aims to optimize the gene sequence of a lon-like protease of L. plantarum IIA-1A5 for heterologous expression system. The Lon-like gene expression system is made using genes that have been optimized first in silico.  pET-28a(+), E. coli BL21(DE3), Nde1 and BamH1 were used in this study as a expression vector, a host and retriction enzyme, respectively.  Molecular weight was validated using SDS-PAGE and expasy.org software. The results showed that optimization increased codon adaptation index value (CAI) and GC content to 0.97 and 56.57%, respectively, which were suitable for the E. coli expression system. The Lon-like IIA gene was successfully expressed in the cell cytoplasm by induction of 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) at 37 °C.  As many as 88% of Lon-like IIA codons were distributed in the 91-100 quality group. Lon-like IIA was successfully expressed in a host cell induced with 1 mM IPTG at 37oC . IPTG induction was performed at the 3rd hour of incubation with OD600 0.59. In addition, Lon-like IIA molecular weight was detected approximately 43 kDa.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48423252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat","authors":"Kusnadidi Subekti, Dedi Duryadi Solihin, R. Afnan, A. Gunawan, Cece Sumanri","doi":"10.17969/agripet.v19i2.14876","DOIUrl":"https://doi.org/10.17969/agripet.v19i2.14876","url":null,"abstract":"ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (χ2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.  (The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck) ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (χ2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46717182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hubungan Kadar Progesteron pada Fase Awal Luteal dengan Kematian Embrio pada Sapi Aceh","authors":"B. Panjaitan, Citra Chyntia Helwana, Nellita Meutia, Yusmadi Yusmadi, T. N. Siregar, D. Dasrul, T. A. Tr","doi":"10.17969/agripet.v19i2.14881","DOIUrl":"https://doi.org/10.17969/agripet.v19i2.14881","url":null,"abstract":"ABSTRAK.  Progesteron merupakan hormon yang berperan penting dalam proses pemeliharaan kebuntingan dan dihasilkan oleh corpus luteum. Penelitian ini bertujuan mengetahui hubungan antara kadar hormon progesteron pada fase awal luteal dengan kematian embrio pada sapi Aceh. Dalam penelitian ini digunakan empat ekor sapi betina dewasa berumur 3-5 tahun, bobot badan 150-250 kg, sehat secara klinis, dan memiliki reproduksi normal. Sapi disinkronisasi menggunakan 5 ml prostaglandin F2 alfa (PGF2α) dengan pola penyuntikan ganda berinterval 11 hari. Koleksi sampel darah untuk pengukuran konsentrasi progesteron dilakukan pada hari ke-5, 6, dan 7 pasca inseminasi. Pengukuran konsentrasi progesteron dilakukan menggunakan metode enzymelinked-immunoassay (ELISA), pemeriksaan kebuntingan dan kematian embrio menggunakan metode transrektal ultrasonografi pada hari ke-25 pasca inseminasi. Pemeriksaan diulang setiap 10 hari sampai hari ke-55 pasca inseminasi. Puncak sekresi progesteron pada sapi bunting dengan embrio yang bertahan hidup terdapat pada hari ke-7 (2,082 ng/ml), pada sapi Late Embryonic Mortality (LEM) di hari ke-5 (8,209 ng/ml) dan pada sapi tidak bunting di hari ke-7 (3,051±1,157 ng/ml). Sekresi progesteron sapi LEM pada hari ke-5 sampai dengan ke-7 cenderung menurun sedangkan pada sapi yang bertahan hidup cenderung meningkat.  (Correlation between progesterone levels in early luteal phase and embryonic death  in Aceh cattle) ABSTRACT. Progesterone is an important hormone that functions to maintain pregnancy and is produced by the corpus luteum. The aim of this study was to see a correlation between progesterone and the incidence of embryonic death in Aceh cattle. This study used four adult female cows, 3-5 years old, 150-250 kg body weight, clinically healthy, and have a normal reproduction. The synchronized with 5 ml prostaglandin F2 alfa hormone, and double injection pattern with 11-day intervals. The blood was collected for progesterone measurements on 5th, 6th, 7th day post artificial insemination. Measurement of progesterone concentration was carried out using an enzymelinked-immunoassay (ELISA), while pregnancy and embryo mortality was performed using the trans-rectal ultrasonography method on the 25th day after insemination. The examination was repeated every 10 days until day 55th after insemination. Progesterone secretion peaks in pregnant cows were on day 7th (2.082 ng/ml), in cattle Late Embryonic Mortality (LEM) on day 5th (8.209 ng/ml) and in cattle not pregnant on day 7th (3.051±1.157 ng/ml). The pattern of LEM progesterone secretion on days 5th to 7th tends to decrease while those that survive tend to increase.","PeriodicalId":30910,"journal":{"name":"Jurnal Agripet","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44153715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}