Metamorfosa Journal of Biological Sciences最新文献

Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari 紫外线和阳光照射后血液中的数量和质量

Metamorfosa Journal of Biological Sciences Pub Date : 2023-05-02 DOI: 10.24843/metamorfosa.2023.v10.i01.p19

Putri Arie Prasetyoningrum, I. K. Junitha, Dwi Ariani Yulihastuti

{"title":"Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari","authors":"Putri Arie Prasetyoningrum, I. K. Junitha, Dwi Ariani Yulihastuti","doi":"10.24843/metamorfosa.2023.v10.i01.p19","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p19","url":null,"abstract":"Pemeriksaan Deoxyribonucleic Acid merupakan metode identifikasi primer dalam kasus forensik. Bercak darah yang ditemukan di tempat kejadian perkara seringkali terpengaruh oleh berbagai faktor lingkungan salah satunya sinar ultraviolet dari matahari. Tujuan dari penelitian ini yaitu membandingkan kuantitas dan kualitas DNA hasil ekstraksi dari bercak darah pada pisau pasca paparan sinar ultraviolet dan matahari selama 0, 15 dan 30 hari. Metode yang digunakan yaitu Rancangan Faktorial; sumber sinar (UVA, sinar matahari secara langsung dan tidak langsung); dan lama perlakuan (0, 15 dan 30 hari). Bercak darah dibuat dengan diteteskan 50 ?L sampel darah manusia pada satu sisi mata pisau kemudian diberikan perlakuan. Analisis DNA meliputi ekstraksi dengan Chelex 5%; uji kuantitas dan kualitas DNA dengan spektrofotometer SimpliNano; dan uji kualitas DNA total dengan elektroforesis gel agarose; serta PCR. Analisis data kuantitif menggunakan uji Univariate yang dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa kuantitas DNA pada sampel yang dipaparkan sinar UVA dan matahari (secara langsung) mengalami peningkatan pada hari ke-15 akibat fragmentasi DNA sedangkan sampel yang terpapar sinar matahari (secara tidak langsung) mengalami penurunan kuantitas DNA dengan semakin lamanya perlakuan. Kualitas DNA berupa kemurnian DNA menghasilkan ekstrak DNA yang tidak murni. Kualitas DNA total dengan elektroforesis gel agarose pada semua sampel menunjukkan bahwa semakin lamanya perlakuan (0, 15 dan 30 hari) menghasilkan pita pendaran DNA yang semakin redup atau tipis dengan smear.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49009398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tabel Hidup Lalat Jamur Tiram, Bradysia ocellaris Bradysia ocellaris活页片

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-30 DOI: 10.24843/metamorfosa.2023.v10.i01.p18

R. Rostaman

引用次数: 0

Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Merah dan Cokelat Terhadap Pertumbuhan Bakteri Staphylococcus aureus 抗细菌提取物。红色和棕色对球菌菌的生长

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-30 DOI: 10.24843/metamorfosa.2023.v10.i01.p17

Luh Made Ary Somia Vagestini, Retno Kawuri, M. R. Defiani

{"title":"Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Merah dan Cokelat Terhadap Pertumbuhan Bakteri Staphylococcus aureus","authors":"Luh Made Ary Somia Vagestini, Retno Kawuri, M. R. Defiani","doi":"10.24843/metamorfosa.2023.v10.i01.p17","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p17","url":null,"abstract":"Staphylococcus aureus dikenal sebagai bakteri yang kerap kali menyebabkan penyakit akibat infeksi pada saluran pernafasan di Indonesia. Bakteri ini diketahui telah mengalami resistensi terhadap beberapa antibiotik. Resistensi S. aureus dapat ditanggulangi dengan mengembangkan antibiotik alami salah satunya yang berasal dari tumbuhan ketapang. Tujuan dilakukannya penelitian ini ialah untuk menganalisa perbandingan aktivitas antibakteri esktrak etanol daun ketapang merah dan daun ketapang cokelat terhadap pertumbuhan bakteri S.aureus, mengetahui MIC dari kedua ekstrak, serta mengetahui golongan senyawa pada ekstrak. Metode penelitian yang dilakukan meliputi ekstraksi sampel dengan metode maserasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri ekstrak dilakukan pada konsentrasi 20%, 15%,10%, dan 5% (b/v) dengan metode cakram difusi pada media Nutrient Agar, serta dilakukan uji fitokimia secara kualitatif. Hasil penelitian menunjukan perbedaan warna daun mempengaruhi daya hambat terhadap bakteri S.aureus. Daya hambat terbaik diperoleh pada ekstrak ketapang merah konsentrasi 20% (b/v) dengan diameter zona hambat 13,67±0,76 mm. Hasil pengujian MIC menunjukan nilai MIC ekstrak ketapang merah diperoleh pada konsentrasi 5% (b/v) dengan daya hambat 1,47±0,25 mm dan pada ekstrak daun ketapang cokelat diperoleh pada konsentrasi 10% (b/v) dengan nilai diameter zona hambat 1,73±0,25 mm. Uji fitokimia ekstrak menunjukan kedua ekstrak mengandung senyawa steroid, alkaloid, tannin, saponin, dan flavonoid namun senyawa terpenoid hanya ditemukan pada ekstrak daun ketapang merah. \u0000Kata Kunci: metabolit sekunder, MIC, resistensi, warna daun ketapang","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46094213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analisis Kualitas Air Di Sungai Telagawaja Kabupaten Karangasem, Bali

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-25 DOI: 10.24843/metamorfosa.2023.v10.i01.p16

Juan Aldo Jaya Pradipta Sitepu, N. L. Watiniasih, A. Dewi

引用次数: 0

Formulasi Body Mask dari Daun Pegagan (Centella asiatica L.) untuk Perawatan Kulit 积雪草皮肤护理面膜

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-11 DOI: 10.24843/metamorfosa.2023.v10.i01.p15

Stareast Wulandari Sigiro, N. L. Arpiwi, N. M. S. Parwanayoni

{"title":"Formulasi Body Mask dari Daun Pegagan (Centella asiatica L.) untuk Perawatan Kulit","authors":"Stareast Wulandari Sigiro, N. L. Arpiwi, N. M. S. Parwanayoni","doi":"10.24843/metamorfosa.2023.v10.i01.p15","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p15","url":null,"abstract":"Body mask is one of the cosmetic preparations used in the last process in skin care. Skin care, especially on the skin of the body, requires moisturizing cosmetics. Therefore it is necessary to take care to keep the skin clean and healthy, one of the skin care is with a body mask. Body mask have many benefits, namely providing moisture, tightening the skin, nourishing the skin, softening the skin, cleaning pores and brightening skin tone. One of the natural ingredients that can be used for skin care is gotu kola leaf (Centella asiatica L.). Gotu kola extract has one of the ingredients, namely asiaticoside, which can stimulate collagen in skin tissue. The purpose of this study was to determine the phytochemical content of Gotu kola leaves, to determine the formulation of a good gotu kola leaf powder mask preparation, and to find out the best formulation according to probandus. The methods used in this research are phytochemical test, making body mask, pH test, organoleptic test, and hedonic test. The results of the research that has been carried out are the phytochemical tests of steroids, saponins, flavonoids, and tannins obtained positive results. The quality of the body mask produced is pH 5.41, and does not cause skin irritation. Organoleptic test of fine texture, green tea scent, greenish white color. The formulation body mask most preferred by probandus is to use a base with added 45 g (F2) of gotu kola leaf powder. \u0000Keywords: Body mask, gotu kola leaves (Centella asiatica L.), skin care.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48876409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skrining Aktinomisetes Pendegradasi Nikotin Pada Daun Tembakau (Nicotiana tabacum L.) 烟草公司（Nicotiana tabacum L.）

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-11 DOI: 10.24843/metamorfosa.2023.v10.i01.p14

Esti Utarti, Dina Amalia Syahidah, Sattya Arimurti

引用次数: 0

Induksi Nanobubbles (NBs) untuk Pertumbuhan Anggrek Dendrobium Imelda Marina Masagung X Bumi Menangis

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-10 DOI: 10.24843/metamorfosa.2023.v10.i01.p13

Tintrim Rahayu, Gatra Ervi Jayanti, Ari Hayati

{"title":"Induksi Nanobubbles (NBs) untuk Pertumbuhan Anggrek Dendrobium Imelda Marina Masagung X Bumi Menangis","authors":"Tintrim Rahayu, Gatra Ervi Jayanti, Ari Hayati","doi":"10.24843/metamorfosa.2023.v10.i01.p13","DOIUrl":"https://doi.org/10.24843/metamorfosa.2023.v10.i01.p13","url":null,"abstract":"Nanobubbles (NBs), nanotechnology has been applied in several fields, such as health, waste management, agriculture, and others. This nanotechnology utilizes the gas in the bubbles, making it easier for the gas to enter the cell. This study aimed to determine the effect of O2, CO2, N2, H2, and O3 gases in NBs on the growth of the Dendrobium Imelda Marina Masagung ´ Bumi Menangis orchid. The method used was to provide fertilizer and NBs treatment to Dendrobium orchids for six weeks with observations every one week. The data obtained were analyzed by two-way ANOVA and further tested post hoc tests with Ptukey <0.05. The results showed significant differences in stem diameter and orchid plant height. The orchid stem diameter was more significant with the NBs treatment than the fertilizer and control treatments. Orchid plants with NBs treatment were higher than those with fertilizer and control treatments. The appearance of new shoots and roots also indicates plant growth. Orchid leaf colour with fertilizer and NBs treatment changed from the Yellow-Green Group (144) to the Green Group (143), except for the control. Leaves colour affects the process of photosynthesis, the green colour will carry out photosynthesis better than Green-Yellow leaves. This affects plant growth in Dendrobium Imelda Marina Masagung ´ Bumi Menangis orchid. NBs enlarge the stem, increase the height and make the leaves green.","PeriodicalId":30806,"journal":{"name":"Metamorfosa Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43696800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analisis Toksisitas Dan Aktivitas Antioksidan Pada Ekstrak Gagang Cengkeh (Syzygium aromaticum) 对丁香柄提取物的毒性和抗氧化活性进行分析

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-10 DOI: 10.24843/metamorfosa.2023.v10.i01.p12

Y. Safitri, F. Fatimah

引用次数: 0

Aplikasi Substitutif Ekstrak Kulit Batang Kelor (Moringa oleifera L.) Dengan Batang Kemangi (Ocimum basilicum L.) Terhadap Mortalitas Larva Nyamuk Aedes aegypti 躯干皮肤提取物(Moringa oleifera L)的替代应用程序。罗勒干杆(Ocimum basilicum L)埃及伊蚊幼虫的死亡率

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-10 DOI: 10.24843/metamorfosa.2023.v10.i01.p11

V. M. Ati, Paula Mds Mau, Ermelinda D Meye, Jeffry Amalo

引用次数: 0

Original review of specificity in the interaction between pathogen invasion and host organism 病原体入侵与宿主相互作用特异性的初步综述

Metamorfosa Journal of Biological Sciences Pub Date : 2023-04-08 DOI: 10.58473/jbs0022

Hua Liu

引用次数: 0