Fave Seccion Ciencias Agrarias最新文献_第7页

EVALUACIÓN DEL PERFIL DE VIRULENCIA DE Campylobacter TERMOTOLERANTES AISLADOS DE GRANJAS COMERCIALES DE POLLOS PARRILLEROS 从商业肉鸡养殖场分离的耐热弯曲杆菌毒力谱评价

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10936

E. Rossler

{"title":"EVALUACIÓN DEL PERFIL DE VIRULENCIA DE Campylobacter TERMOTOLERANTES AISLADOS DE GRANJAS COMERCIALES DE POLLOS PARRILLEROS","authors":"E. Rossler","doi":"10.14409/favecv.v19isuplemento.10936","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10936","url":null,"abstract":"The aim of this work was to analyze the presence of genes associated with the virulence of thermotolerant Campylobacter (CT) isolated from different sources in commercial broiler farms. For this, we worked with a collection of CT isolates obtained from three commercial broiler farms during 2015. The presence of 10 genes related to CT virulence was examined with a PCR reaction. The results showed a prevalence of 100% for flaA and flhA; 91% cadF, 51% cdtABC, 48% iam, 46% racR, 32% ciaB and 11% virB11. C. jejuni showed higher prevalence of all genes evaluated than C. coli, except iam and virB11 wich were higher for C. coli. The same gene pattern was presented in different sources of isolates. The high prevalence of flaA and flhA, involved in CT mobility, cadF related to Campylobacter gut colonization of broiler and cdtABC genes involved for the expression of cytotoxin, indicates the importance of these factors in CT virulence. Differences in the prevalence of genes according to the species of CT could demonstrate different mechanisms of pathogenicity, and this is not related to the source of isolates. All the results showed the ability of CT to colonize cells but is not evidence of pathogenesis.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84509600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SENSIBILIDAD DE LA GARRAPATA COMÚN DEL BOVINO Rhipicephalus (Boophilus) microplus A IVERMECTINA ESTIMADA CON EL MODELO SIGMOIDEO DE RESPUESTA MÁXIMA O DE HILL 用SIGMOIDEO最大反应或HILL模型估计牛蜱对伊维菌素的敏感性

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10943

J. Torrents

引用次数: 0

CRECIMIENTO Y CARACTERÍSTICAS FERMENTATIVAS DE Kluyveromyces marxianus EN MATRICES DERIVADAS DE LACTOSUERO

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10941

G. A. Gómez

{"title":"CRECIMIENTO Y CARACTERÍSTICAS FERMENTATIVAS DE Kluyveromyces marxianus EN MATRICES DERIVADAS DE LACTOSUERO","authors":"G. A. Gómez","doi":"10.14409/favecv.v19isuplemento.10941","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10941","url":null,"abstract":"Milk whey has a high content of organic matter; therefore, uncontrolled discharge can cause environmental problems. An alternative for whey utilization is the production of fermented alcoholic beverages by means of the yeast Kluyveromyces marxianus, able to use lactose as a carbon source. Four different liquid growth media were prepared: whey powder (SP) and demineralized whey powder (SPD) (65 g/L) dissolved in sterilized distilled water; SPCal, similar to SP in composition, but sterilized and centrifuged to remove proteins, and milk permeate (PER). All media were fermented by 24 hours at 30°C. Samples were taken every 8 hours, for pH, biomass, ethanol and lactose determination. K. marxianus (LFIQK1 strain) growth was similar in SP, SPD and SP Cal (biomass between 2 – 3 g/L) and lower in PER. Moderate pH decline was observed, final values being between 4,7 and 5,4. Significant lactose consumption was observed in every growth media, with final values around 12 g/L and good ethanol production, especially for SP, SPD, and PER mediums (17,0±0,7; 17,6±0,5; 16,3±0,2 g/L respectively). These results are encouraging for the production of alcoholic beverages from the studied media using K. marxianus as the fermentation agent.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79751094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DESARROLLO DE MODELO MURINO DE COLONIZACIÓN DE Lactobacillus plantarum LP5 DE ORIGEN PORCINO 猪源植物乳杆菌LP5定殖小鼠模型的建立

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10938

M. J. Ruiz

{"title":"DESARROLLO DE MODELO MURINO DE COLONIZACIÓN DE Lactobacillus plantarum LP5 DE ORIGEN PORCINO","authors":"M. J. Ruiz","doi":"10.14409/favecv.v19isuplemento.10938","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10938","url":null,"abstract":"The objective of this work was to generate an experimental model of colonization by L. plantarum LP5 in mice (Mus musculus) aimed at improving the general state of the animal and preventing the possible presence of zoonotic pathogens. The mice used were females of the Balb/cCmedc strain, 6 weeks old. Animals were organized into 2 groups of 3 individuals: treated (GT) and control (GC). GT was administered twice weekly by gavage with 100 µl of rifampicin resistant porcine L. plantarum LP5 at a concentration of 1010 CFU. Mice were fed water and food ad libitum throughout the experiment. Colonization in both groups was evaluated by the presence of clinical signs and by a microbiological analysis of the feces. Sampling was performed at weekly intervals to quantify the populations of the supplied L. plantarum LP5, Lactobacillus spp., Escherichia coli, total coliforms, Campylobacter and yeast. The results of the sampling in the GT in the week showed a recovery of L. plantarum LP5 above 5 logUFC/g, in the second it was 7 logUFC/g and in the third it was 5 logUFC/g. The CG, meanwhile, maintained the null count throughout the experiment. The results showed that there was no imbalance between the microbial components. Therefore, it is feasible to affirm that the inoculum did not alter the balance of the intestinal ecosystem. Particularly, it did not exert an inhibitory effect on the members of the previously established microbiota.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85036764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETERMINACIÓN DE LA CONCENTRACIÓN EFECTIVA 50, 90 Y 99 DE IVERMECTINA SOBRE UNA CEPA DE GARRAPATA COMÚN DEL BOVINO (Rhipicephalus (Boophilus) microplus) PROVENIENTE DE LA PROVINCIA DE SANTA FE-ARGENTINA 伊维菌素对阿根廷圣菲省一种常见牛蜱(牛蜱)的有效浓度50、90和99的测定

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10944

J. Torrents

{"title":"DETERMINACIÓN DE LA CONCENTRACIÓN EFECTIVA 50, 90 Y 99 DE IVERMECTINA SOBRE UNA CEPA DE GARRAPATA COMÚN DEL BOVINO (Rhipicephalus (Boophilus) microplus) PROVENIENTE DE LA PROVINCIA DE SANTA FE-ARGENTINA","authors":"J. Torrents","doi":"10.14409/favecv.v19isuplemento.10944","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10944","url":null,"abstract":"The aim of this study was to obtain the sensitivity data of the native strains of R. microplus to ivermectin (IVM) in the province of Santa Fe-Argentina, in order to detect the emergence of resistant strains. Native (field isolation) and susceptible (reference) larvae of R. microplus were treated with increasing concentrations of IVM in order to determine its efficacy at different doses. The statistical treatment of the concentration-efficacy curves was performed by the PROBIT model, who is a standardized method proposed by FAO. The results obtained indicated that the larvae isolated were susceptible, being the values of LD50 (9.66 ppm) and LD90 (17.41 ppm), which were lower than those of the reference strain (LD50:13.58 ppm, LD90:19.31 ppm), and the RR (resistance ratio) were less than one (RR50: 0.711; RR90: 0.901). The DL99 (51.48 ppm) was only obtained in the reference strain to determine the discriminant dose (DL99x2), which is a parameter that could be used to easily discriminate susceptible and resistant larvae populations.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73403271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

APLICACIÓN DE COLORACIONES ESPECIALES EN EL DIAGNÓSTICO HISTOPATOLÓGICO: TINCIÓN TRICRÓMICA DE MASSON 特殊染色在组织病理学诊断中的应用:马森三色染色

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10932

N. Cruz

{"title":"APLICACIÓN DE COLORACIONES ESPECIALES EN EL DIAGNÓSTICO HISTOPATOLÓGICO: TINCIÓN TRICRÓMICA DE MASSON","authors":"N. Cruz","doi":"10.14409/favecv.v19isuplemento.10932","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10932","url":null,"abstract":"Masson's trichrome (MT) is a three-color staining protocol used in histology. MT allows to show and quantify changes such as tissue repair (healing) and collagen deposition. Also, it can be used to quantify blood vessels, in epithelial dysplasia and squamous cell carcinoma. The objective of this work is to describe the MT staining technique and to exemplify some applications of this technique in routine veterinary histopathological diagnosis. Archived histologic sections were selected from the records of the histopathology laboratory. Tissues were selected in base on theirs structures and lesions that could be evaluated with MT: a rabbit lung with a chronic suppurative bronchopneumonia; a bovine liver with lesions of Echium plantagineum poisoning; and a bovine eyelid with a squamous cell carcinoma. The TM was able to show fibroplasia in the pulmonary interstitium and confirm the presence of a chronic respiratory process, and clearly revealed an abundant fibrovascular tumor stroma, with profuse connective tissue and neovascularization between the tumor cells in deep dermis. In the liver, extensive and marked fibroplasia was confirmed. MT represents a complementary coloration to routine hematoxylin and eosin stain (H&E) and provides accurate information from several pathological processes, mainly those related to fibrovascular proliferation and scarring.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75961647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PROPUESTA DE UNA METODOLOGÍA PARA CUANTIFICAR LA ACTIVIDAD ANTIBACTERIANA DE LA MIEL 提议的方法量化活动感染性蜜

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10934

A. Ferrer

{"title":"PROPUESTA DE UNA METODOLOGÍA PARA CUANTIFICAR LA ACTIVIDAD ANTIBACTERIANA DE LA MIEL","authors":"A. Ferrer","doi":"10.14409/favecv.v19isuplemento.10934","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10934","url":null,"abstract":"In this study a standardized method for objectively comparing the antibacterial activity of honey was developed. The assay was performed using a strain of S. aureus and four honeys that have been shown antibacterial activity against this strain. The antibacterial activity of honeys were checked by using an agar plate diffusion method using S. aureus (ATCC 29213) as the test organism. On the surface of each agar plate six stainless steel cylinders with a diameter of 8,14 mm were radially disposed. Onto each cylinder 0,1 mL of sample honey at 10% v/v was placed and all Petri dishes were incubated at 35°C during 24 h. Each sample were analyzed by triplicate. The surface of the cylinder diameter was set as reference antibacterial activity equivalent to minimal inhibitory concentration (MIC). The antibacterial activity of each honey was quantitated by the relationship between the surface of the inhibitory zone and the surface of the reference activity expressed as multiples of MIC (x MIC). The method allowed establishing differences between the bacterial activities of the different honeys tested and could be used for identifying honeys that could be employed with medicinal purpose for the treatments of wounds.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79324401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CORRECCIÓN DEL DESPLAZAMIENTO ABOMASAL IZQUIERDO POR VIDEOLAPAROSCOPÍA 腹腔镜矫正左胃移位

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-06 DOI: 10.14409/favecv.v19isuplemento.10935

C. Leiva

引用次数: 0

TRICHUROSIS EN TERNEROS EN EL DEPARTAMENTO LAS COLONIAS, SANTA FE 圣达菲LAS COLONIAS部门的小牛旋毛虫病

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-02 DOI: 10.14409/favecv.v19isuplemento.10914

M. F. Bono Battistoni

引用次数: 0

ANÁLISIS DE MINERALES SÉRICOS DE CERDAS EN LACTACIÓN EN DOS GRANJAS COMERCIALES 两个商业农场泌乳母猪血清矿物质分析

Fave Seccion Ciencias Agrarias Pub Date : 2021-12-02 DOI: 10.14409/favecv.v19isuplemento.10917

J. Bellezze

{"title":"ANÁLISIS DE MINERALES SÉRICOS DE CERDAS EN LACTACIÓN EN DOS GRANJAS COMERCIALES","authors":"J. Bellezze","doi":"10.14409/favecv.v19isuplemento.10917","DOIUrl":"https://doi.org/10.14409/favecv.v19isuplemento.10917","url":null,"abstract":"Mineral nutrition is essential for profitable production. Health affected is not provided proper levels minerals. Objective of the present work was to analyze mineral profile in serum of sows in lactation, province Santa Fe (A), Entre Rios (B) and fed with diets minerals of inorganic origin. Blood serum samples 68 sows, Farm A (in field) and Farm B (in stabled form) were analyzed. Mineral concentrations were determined by means of atomic absorption spectrophotometry; ANOVA was applied to these data. Mean values and their standard deviations obtained are: Calcium (Ca) (mg/dL): 8,61±3,44/ 8,11± 1,73; Phosphorus (P) (mg/dL): 2,66a±0,90/ 3,53b± 1,16; Magnesium (Mg) (mg/dL): 2,25a±0,30/ 1,89b±0,35; Sodium (Na) (mEq/L): 160,98a±15,47/ 138,53b±9,48; Potassium (K) (mEq/L): 3,42±0,24/ 3,58±0,33; Iron (Fe) (µg/dL): 62,74a±6,07/ 94,82b±19,19; Copper (Cu) (ppm): 1,63a±0,35/ 1,01b±0,32; Zinc (Zn) (ppm): 1,82a±0,5/ 1,50b±0,42, for farms A and B respectively. Na and Zn averages were observed slightly above upper limit of normal range. Copper in farm B was observed below normal range. Significant difference (“a” and “b” P <0.05) in P, Mg, Na, Fe, Cu and Zn was observed between two farms, this is due to difference in gestation system. Cu concentration tended to be less. Is that subject should continue to be studied.","PeriodicalId":30802,"journal":{"name":"Fave Seccion Ciencias Agrarias","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85556965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0