伊维菌素对阿根廷圣菲省一种常见牛蜱(牛蜱)的有效浓度50、90和99的测定

J. Torrents
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引用次数: 0

摘要

本研究的目的是获取阿根廷圣菲省本地微孢子虫对伊维菌素(IVM)的敏感性数据,以检测耐药菌株的出现。采用不同剂量的IVM,对本地(田间分离)和敏感(对照)小夜蛾幼虫进行处理,以确定不同剂量的IVM效果。浓度-功效曲线的统计处理采用PROBIT模型,PROBIT模型是FAO提出的标准化方法。结果表明,分离出的幼虫对该病菌敏感,LD50 (9.66 ppm)和LD90 (17.41 ppm)均低于对照菌株(LD50:13.58 ppm、LD90:19.31 ppm),抗性比小于1 (RR50: 0.711;RR90: 0.901)。DL99 (51.48 ppm)是判别剂量(DL99x2)的重要参数,该参数可方便地区分易感和抗性幼虫种群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DETERMINACIÓN DE LA CONCENTRACIÓN EFECTIVA 50, 90 Y 99 DE IVERMECTINA SOBRE UNA CEPA DE GARRAPATA COMÚN DEL BOVINO (Rhipicephalus (Boophilus) microplus) PROVENIENTE DE LA PROVINCIA DE SANTA FE-ARGENTINA
The aim of this study was to obtain the sensitivity data of the native strains of R. microplus to ivermectin (IVM) in the province of Santa Fe-Argentina, in order to detect the emergence of resistant strains. Native (field isolation) and susceptible (reference) larvae of R. microplus were treated with increasing concentrations of IVM in order to determine its efficacy at different doses. The statistical treatment of the concentration-efficacy curves was performed by the PROBIT model, who is a standardized method proposed by FAO. The results obtained indicated that the larvae isolated were susceptible, being the values of LD50 (9.66 ppm) and LD90 (17.41 ppm), which were lower than those of the reference strain (LD50:13.58 ppm, LD90:19.31 ppm), and the RR (resistance ratio) were less than one (RR50: 0.711; RR90: 0.901). The DL99 (51.48 ppm) was only obtained in the reference strain to determine the discriminant dose (DL99x2), which is a parameter that could be used to easily discriminate susceptible and resistant larvae populations.
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