{"title":"The biochemical composition of Cylindrotheca closterium (Ehrenb.) Rayman net Levin, as a source of biologically active substances in cumulative cultivation","authors":"S. Zheleznova, R. Gevorgiz","doi":"10.33624//2311-0147-2021-1(25)-1-9","DOIUrl":"https://doi.org/10.33624//2311-0147-2021-1(25)-1-9","url":null,"abstract":"In this work, we studied the biochemical characteristics of the diatom Cylindrotheca closterium in the cumulative mode of cultivation in different phases of growth. According to our data, in the exponential growth phase, C. closterium biomass is characterized by a minimum lipid content of 3% of dry biomass and a maximum carbohydrates content of 23% of dry weight. During the transition of C. closterium culture to the stationary growth phase, as in the case of a representative of diatoms, a rather high accumulation of total lipids is observed – to 28% dry weight, while the carbohydrates content decreases to 7%. At the end of the stationary phase (21st day of cultivation), the content of fatty acids is 50% of total lipids or 12.5% of dry weight. A certain relationship between the concentrations of fucoxanthin, total lipids and polyunsaturated fatty acids (PUFA) in the biomass of C. сlosterium diatom was recorded. During active growth in the exponential phase, the concentration of fucoxanthin was 0.5–1 mg∙g-1 dry weight. During the tranition of the culture to the stationary phase of growth, the concentration of fucoxanthin reached 7±0.2 mg∙g-1 dry biomass. At the end of the stationary phase, the concentration of fucoxanthin reached its maximum value of 20 mg∙g-1 dry weight.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120948909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ludikova, T. Sapelko, D. D. Kuznetsov, M. A. Naumenko, V. Boynagryan
{"title":"Evolution and a current state of high-mountain Lake Kari (Armenia) as inferred from the diatom study","authors":"A. Ludikova, T. Sapelko, D. D. Kuznetsov, M. A. Naumenko, V. Boynagryan","doi":"10.33624/2311-0147-2019-2(20)-229-232","DOIUrl":"https://doi.org/10.33624/2311-0147-2019-2(20)-229-232","url":null,"abstract":"The paper present the results of the diatom study of a sediment core and surface-sediment samples from a high-mountain Lake Kari, Armenia. The results enabled to infer the main stages of the lake’s evolution throughout the last 4000 yrs, and characterize its current state.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121311753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. A. Bobrovnikova, M. S. Pakholkova, R. Sidorov, M. Sinetova
{"title":"Starch and triacylglycerol accumulation in the cells of the stain Chlorella sp. IPPAS C-1210","authors":"L. A. Bobrovnikova, M. S. Pakholkova, R. Sidorov, M. Sinetova","doi":"10.33624/2311-0147-2021-2(26)-1-7","DOIUrl":"https://doi.org/10.33624/2311-0147-2021-2(26)-1-7","url":null,"abstract":"Strain Сhlorella sp. IPPAS C-1210 is an effective lipid and triacilglycerols (TAG) producer. The strain could be used eventually in such industries as bioenergetics, food industry and agriculture. The objective of this work was investigation of conditions in which the strain Сhlorella sp. IPPAS C-1210 accumulates the most starch and TAG in cells with a view to optimise its growth and productivity. The following cultivation parameters were investigated in order to figure out their influence on accumulation of starch and TAG: nitrogen- and phosphorous-starvation and cultivation on media with different nitrogen (nitrate, urea) and carbon (carbon dioxide, bicarbonate) sources. Pigments, starch, protein and lipid content in cells were measured. The exclusion of nitrogen or phosphorus source from medium decreased the biomass productivity significantly, caused chlorosis and reduction of protein content. Total lipid content increased slightly after phosphorous starvation and stayed almost constant under nitrogen starvation, however a greater TAG increase was observed during nitrogen starvation. Both nitrogen and phosphorous starvations caused the increase of the amount of reserve carbohydrates: during phosphorous starvation increase was insignificant, whereas the latter almost doubled the amount of reserve carbohydrates. The highest biomass and lipid productivity was observed in cells grown in bicarbonate supplement medium and the highest starch productivity was observed in cells grown in standard BBM-3N medium.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122478044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Review of the long term research results using the author's method of graphical analysis","authors":"L. Razumovsky","doi":"10.33624/2311-0147-2019-2(20)-69-73","DOIUrl":"https://doi.org/10.33624/2311-0147-2019-2(20)-69-73","url":null,"abstract":"The work describes new method of graphical analysis developed for freshwater ecosystems and three scenarios of their transformation were documented. The parameters of the structure-forming groups of hydrobionts, which determine their stability, are established. The transformations recorded in natural or natural-technogenic systems were analyzed using a similar method. The similarity of those scenarios allows us to refer all the considered systems to the systems of the fourth level of complexity.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124136086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nitrogen-fixing cyanobacteria in the mountain-tundra ecosystems of the northern regions of the Urals (diversity, functional characteristics)","authors":"E. Patova, M. Sivkov","doi":"10.33624/2311-0147-2022-2(29)-57-64","DOIUrl":"https://doi.org/10.33624/2311-0147-2022-2(29)-57-64","url":null,"abstract":"In biological soil crusts (BSC) of mountain tundras of three northern regions of the Urals, 131 species of cyanobacteria from 46 genera were identified. Nitrogen fixers make up about 40% of the total diversity. The complex of dominants is formed by representatives of the genera Nostoc, Stigonema, Scytonema. Based on the study of the nitrogen-fixing activity of biological crusts dominated by cyanobacteria, two groups were distinguished: those with high and low rates of nitrogen fixation. High rates of nitrogen fixation, on average 3.17 mg C2H4 m-2h-1, are typical for BSC dominated by Nostoc commune Vaucher ex Bornet & Flahault or Stigonema ocellatum Thuret ex Bornet & Flahault, low rates, on average 1.01 mg C2H4 m-2h-1, for BC dominated by Stigonema minutum Hassall ex Bornet et Flahault. It has been shown that the nitrogen fixation of the studied BSCs depends to a greater extent on the composition of the dominant cyanobacteria species in soil crusts than on the geographic zone in which the samples were collected.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115283793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Taxonomic diversity of the dominant cyanobacteria species during blooms in water bodies of Russia","authors":"Z. Namsaraev, A. Melnikova","doi":"10.33624/2311-0147-2022-2(29)-29-37","DOIUrl":"https://doi.org/10.33624/2311-0147-2022-2(29)-29-37","url":null,"abstract":"An analysis of information about the dominant cyanobacteria taxa during cyanobacterial blooms in the largest water bodies of Russia during the summer period was carried out. As a result, 32 species of cyanobacteria belonging to 17 genera were shown to be among the dominant species in inland water bodies. Of these, Aphanizomenon flos-aquae, Microcystis aeruginosa, Dolichospermum flos-aquae and Dolichospermum lemmermannii were the most frequently encountered taxa during blooms. In marine systems, representatives of 12 species belonging to 9 genera were observed. Among them Aphanizomenon flos-aquae, Trichormus variabilis, Dolichospermum flos-aquae, Microcystis aeruginosa and Nodularia spumigena were the most common. It is noted that Aphanizomenon flos-aquae, Microcystis aeruginosa and Dolichospermum flos-aquae have the widest distribution over the territory of Russia and are found both in water bodies of the Arctic zone of the Russian Federation and the south of the country, as well as in the distributed parts of coastal seas, while Dolichospermum lemmermannii is more typical of water bodies located in regions with subarctic or boreal climate, and is not found as a dominant in regions with temperate climate.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132474277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fossil diatoms from the Volga Delta Holocene sediments (Damchik region) as indicators of the paleoecological conditions of sedimentation","authors":"Yelena I. Shtyrkova, Y. Polyakova","doi":"10.33624/2311-0147-2019-2(20)-270-273","DOIUrl":"https://doi.org/10.33624/2311-0147-2019-2(20)-270-273","url":null,"abstract":"The results of fossil diatoms investigation from the deltaic sediments are presented. Samples were obtained from the core DM-1 and two Holocene outcrops from the Damchik region of the Astrakhan Nature Reserve. In the core samples eight periods of sedimentation based on diatom analysis were identified: the sediments formed in shallow freshwater basins and deltaic channels. The samples from the outcrops were investigated in much greater detail.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132529306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Is the type of sexual process a generic characteristic?","authors":"O. Davidovich, N. Davidovich","doi":"10.33624/10.33624/2311-0147-2019-2(20)-190-193","DOIUrl":"https://doi.org/10.33624/10.33624/2311-0147-2019-2(20)-190-193","url":null,"abstract":"The study of sexual reproduction in members of the pennate diatoms of the genus Haslea (H. ostrearia, H. karadagensis, H. provincialis, H. crucigera, H. subagnita) showed that they all have the same type of sexual reproduction. Our and literature data suggest that the scheme of the process of sexual reproduction is a conservative generic characteristic.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131935868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study of marine Paleogene diatom assemblages from the Kamchatka region: results of the last decade","authors":"A. Gladenkov","doi":"10.33624/2311-0147-2022-1(28)-117-122","DOIUrl":"https://doi.org/10.33624/2311-0147-2022-1(28)-117-122","url":null,"abstract":"Review of results obtained on fossil diatom assemblages studied from marine Paleogene sections in Kamchatka region in the last decade are presented. The first data for Kamchatka on direct correlation of Eocene diatom assemblages with complexes of calcareous plankton (the Il’pinskii Peninsula stratigraphic section, northeast Kamchatka), and on correlation of Oligocene diatom assemblages with the magnetostratigraphic scale (the Kvachina Bay section, west Kamchatka) are emphasized.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130781062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Kezlya, Y. Maltsev, A. Glushchenko, M. Kulikovskiy
{"title":"Phylogeny and morphology of some species from the genus Gomphonema Ehrenberg","authors":"E. Kezlya, Y. Maltsev, A. Glushchenko, M. Kulikovskiy","doi":"10.33624/10.33624/2311-0147-2019-2(20)-181-183","DOIUrl":"https://doi.org/10.33624/10.33624/2311-0147-2019-2(20)-181-183","url":null,"abstract":"New molecular data on diatoms from the genus Gomphonema Ehrenberg are analyzed.","PeriodicalId":305989,"journal":{"name":"Issues of modern algology (Вопросы современной альгологии)","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114144409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}