Cellular, Molecular and Biomedical Reports最新文献_第5页

Clinical analysis of diagnosing a case with tuberculous peritonitis from Afghanistan 阿富汗结核性腹膜炎1例临床分析

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.354911.1053

引用次数: 1

Overview of typing techniques as molecular epidemiology tools for bacterial characterization 分型技术作为细菌表征的分子流行病学工具概述

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.143413.1016

Nasim Almasian-Tehrani, M. Alebouyeh, S. Armin, N. Soleimani, L. Azimi, Roozbeh Shaker-Darabad

{"title":"Overview of typing techniques as molecular epidemiology tools for bacterial characterization","authors":"Nasim Almasian-Tehrani, M. Alebouyeh, S. Armin, N. Soleimani, L. Azimi, Roozbeh Shaker-Darabad","doi":"10.55705/cmbr.2021.143413.1016","DOIUrl":"https://doi.org/10.55705/cmbr.2021.143413.1016","url":null,"abstract":"The main purpose of microbial typing is to evaluate the relationships between microbial isolates. Microbial typing can use for identifying the source of infection by detecting a clonal link between the strains. Moreover, it can analyze outbreaks, antimicrobial-resistant strains, and evaluate the effectiveness of control measures so, the efficiency of monitoring systems would increase. HAIs can affect hospitalized patients in all age ranges with any clinical situation, and lead to death. Molecular epidemiology is useful to determine genetic relatedness between isolated pathogens from patients, and design proper prevention plans to prevent infection through the hospital and community. Nowadays, typing methods for a wide range of bacterial strains are known as essential epidemiological tools to prevent and control infections in hospitals and communities. Although basic typing methods were more focused on phenotypic techniques like antibiogram and serotyping, new methods are based on molecular techniques including PCRbased methods and sequencing-based methods. Due to the high frequency of methods, choosing the right one for research applications seems difficult and requires basic knowledge about all of them. In this review, we aim to introduce the most useful and practical molecular typing techniques. Also, their utilization, advantages, and disadvantages were compared. Article info Received: 18 June 2021 Revised: 06 Aug 2021 Accepted: 18 Nov 2021 Use your device to scan and read the article online","PeriodicalId":304796,"journal":{"name":"Cellular, Molecular and Biomedical Reports","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117201675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Antisense RNA, the modified CRISPR-Cas9, and metal/metal oxide nanoparticles to inactivate pathogenic bacteria 反义RNA、修饰的CRISPR-Cas9和金属/金属氧化物纳米颗粒灭活致病菌

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.142436.1014

Mehran Alavi, M. Rai

{"title":"Antisense RNA, the modified CRISPR-Cas9, and metal/metal oxide nanoparticles to inactivate pathogenic bacteria","authors":"Mehran Alavi, M. Rai","doi":"10.55705/cmbr.2021.142436.1014","DOIUrl":"https://doi.org/10.55705/cmbr.2021.142436.1014","url":null,"abstract":"Finding efficient therapeutic strategies to fight antibiotic-resistant bacteria is a complicated affair specifically in the therapy of chronic bacterial infections related to hospital-acquired infections. Recently, three major antibacterial systems based on antisense RNA, CRISPR-Cas9, and nanoparticles have shown more effective antibacterial activity compared to conventional antibiotics. ROS generation, attachment to the cell membrane, disruption of bacterial envelop, inactivation of electron transport chain, decreasing the local pH, modulation of cell signaling, and denaturation of biological macromolecules such as proteins and nucleic acids have been found as the main antibacterial functions of Ag nanoparticles. Antisense RNA, a single-stranded RNA, can hybridize with complementary genes in messenger RNA (mRNA) followed by blockage translation of these genes into proteins. Moreover, CRISPR (clustered regularly interspaced short palindromic repeats) is a family of viral DNA sequences derived from bacteriophages, which can target and destroy foreign DNA by nuclease activity. There are 2 classes and 6 subtypes (I-VI) of CRISPR-Cas systems, which may be engineered as potential antibacterial agents to target specific sequences. Therefore, here, recent advances and challenges for the antibacterial application of these three therapeutic agents are presented.","PeriodicalId":304796,"journal":{"name":"Cellular, Molecular and Biomedical Reports","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121251654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

The relationship between IL23R 1142G / A (Arg381Gln) and GM-CSF 3928 C / T (Ile117Thr) gene polymorphism in Iranian patients with tuberculosis disease 伊朗肺结核患者IL23R 1142G / A (Arg381Gln)与GM-CSF 3928c / T (Ile117Thr)基因多态性的关系

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.356674.1059

Kolsoum Rezaie-Kahkhaie, Khadije Rezaie-Keikhaie, Leli Rezaie-Kahkhaie, Khadijeh Saravani, A. Kamali

引用次数: 3

Molecular and clinical analysis of genes involved in gastric cancer 胃癌相关基因的分子与临床分析

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.355860.1056

引用次数: 10

An introduction on cerebrovascular aneurysms during pregnancy 妊娠期脑血管动脉瘤的介绍

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.356664.1057

Khadijeh Saravani, Zahra Mirsarzai, Monireh Sekhavati

引用次数: 2

A retrospective observational study to assess the cirrhosis risk in alcoholic patients 一项评估酒精患者肝硬化风险的回顾性观察性研究

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.361362.1063

Paspula Soumya, T. Priya, Hameed Qureshi, Alisha Firdous

{"title":"A retrospective observational study to assess the cirrhosis risk in alcoholic patients","authors":"Paspula Soumya, T. Priya, Hameed Qureshi, Alisha Firdous","doi":"10.55705/cmbr.2021.361362.1063","DOIUrl":"https://doi.org/10.55705/cmbr.2021.361362.1063","url":null,"abstract":"Cirrhosis is a late-stage liver disease in which/ advanced stage of scaring and conditions, such as hepatitis and chronic alcoholism. This disease is a condition in which your liver is scarred and permanently damaged. Scar tissue replaces healthy liver tissue and prevents your liver from working normally. Irreversible change in the normal liver tissue results in the degeneration of functioning liver cells and their replacement with fibrous connective tissue. In the present study, the risk assessment in alcoholic patients is assessed using laboratory data. Other biochemical parameters and severity are assessed using the child-pugh score. In the present study, 98 patients were identified of which 8 patients were excluded due to various reasons, and 90 patients’ data were analyzed. In order to assess the risk of cirrhosis in alcoholic patients, we used the cpugh score for assessing the severity and survival rate of subjects. A total of 90 patients were enrolled in this observational study. The study confirmed that more than 60 % of the subjects possess a severe risk of cirrhosis and risk factors such as age, gender, ALD, high BMI, social history family history, and encephalopathy.","PeriodicalId":304796,"journal":{"name":"Cellular, Molecular and Biomedical Reports","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126660281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Clinical challenges of stress cardiomyopathy during coronavirus 2019 epidemic 2019冠状病毒流行期间应激性心肌病的临床挑战

Cellular, Molecular and Biomedical Reports Pub Date : 2021-12-01 DOI: 10.55705/cmbr.2021.145790.1018

Ebrahim Rahbar-Karbasdehi, Fatemeh Rahbar-Karbasdehi

引用次数: 18

Relevance of genetic polymorphisms of the human cytochrome P450 3A4 in rivaroxaban-treated patients 利伐沙班治疗患者中人类细胞色素P450 3A4基因多态性的相关性

Cellular, Molecular and Biomedical Reports Pub Date : 2021-06-01 DOI: 10.55705/cmbr.2021.138880.1003

M. Ercisli, Lechun Gao, S. Azeez, R. Hamasalih, Siyan Song, Z. Aziziaram

{"title":"Relevance of genetic polymorphisms of the human cytochrome P450 3A4 in rivaroxaban-treated patients","authors":"M. Ercisli, Lechun Gao, S. Azeez, R. Hamasalih, Siyan Song, Z. Aziziaram","doi":"10.55705/cmbr.2021.138880.1003","DOIUrl":"https://doi.org/10.55705/cmbr.2021.138880.1003","url":null,"abstract":"Rivaroxaban is an anticoagulant drug that prevents forming of blood clots. In addition, it can be administered to prevent and treat thrombotic diseases such as atrial fibrillation, cardiac arrhythmia, heart valve disease, orthopedic surgery, and thrombophilia to reduce the risk of thrombosis. Various factors such as age, gender, diet, medications, and genetic factors effectively determine the dose of rivaroxaban. Genetic variability in drug-metabolizing enzymes, including the cytochrome P450 (CYP450) enzymes and especially CYP3A4, has been associated with rivaroxaban response. The current study aimed to identify the frequency of CYP3A4 common polymorphisms, as well as their association with rivaroxaban response in 100 patients of Arab descent (48.6% female). CYP3A4 gene polymorphisms were examined by the PCR-RFLP method, and the findings were analyzed by SPSS 16 software and t-test. The frequency of CYP3A4*1B/*1B, CYP3A4*1B/*1A, CYP3A4*1B/*1C, and CYP3A4*1A/*1C was 67.35%, 10.64%, 19.12% and 2.89%, respectively. According to our results, CYP3A4 *1B/*1B genotype was the most common, and patients with CYP3A4*1B/*1B alleles needed a higher daily dose of rivaroxaban than *1B/*1A, *1B/*1C, and *1A/*1C carriers (9.57 ± 1.54 mg/day, P=0.015). Therefore, according to the results, CYP3A4 gene polymorphism has an important effect on the dose of rivaroxaban required to maintain the International Normalized Ratio (INR) in the range of 2-3.","PeriodicalId":304796,"journal":{"name":"Cellular, Molecular and Biomedical Reports","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125524640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Association between Human Endogenous Retrovirus K gene expression and breast cancer 人内源性逆转录病毒K基因表达与乳腺癌的关系

Cellular, Molecular and Biomedical Reports Pub Date : 2021-06-01 DOI: 10.55705/cmbr.2021.138810.1008

M. Tourang, Le-kun Fang, Yuan Zhong, Ramchandra S. Suthar

引用次数: 50