Depik Jurnal最新文献

筛选
英文 中文
FRONT MATTER 前页
Depik Jurnal Pub Date : 2023-09-20 DOI: 10.13170/depik.12.2.34385
. .
{"title":"FRONT MATTER","authors":". .","doi":"10.13170/depik.12.2.34385","DOIUrl":"https://doi.org/10.13170/depik.12.2.34385","url":null,"abstract":"http://jurnal.usk.ac.id/depik/about/editorialTeam","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136310723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular characterization of Tapah fish (Wallago leerii, Bleeker 1851) from Riau Province based on Cytochrome b gen 基于细胞色素b原的廖内省Tapah鱼(Wallago leerii, Bleeker 1851)分子特征分析
Depik Jurnal Pub Date : 2023-08-31 DOI: 10.13170/depik.12.2.33161
Roza Elvyra, Nurasiah Nurasiah, Agung Setia Batubara
{"title":"Molecular characterization of Tapah fish (Wallago leerii, Bleeker 1851) from Riau Province based on Cytochrome b gen","authors":"Roza Elvyra, Nurasiah Nurasiah, Agung Setia Batubara","doi":"10.13170/depik.12.2.33161","DOIUrl":"https://doi.org/10.13170/depik.12.2.33161","url":null,"abstract":"Tapah fish (Wallago leerii) is a fish with high economic value in Riau Province. Molecular research on W. leerii is very important to do. This study aims to determine the molecular characteristics of W. leerii from Riau Province based on the Cytochrome b gene. The universal primer Cytochrome b is used for the Polymerase Chain Reaction (PCR) process. The PCR result is a partial Cytochrome b gene with a length of 373 bp. Multiple alignments were performed on nucleotide sequences of the Cytochrome b gene of W. leerii from river of Tapung and Indragiri Riau with the cytochrome b gene of other fish from Genbank and analyzed using the MEGA program version 6.0. Phylogenetic construction using the Cytochrome b gene can distinguish W. leerii from river of Tapung and Indragiri Riau, with other species.Keywords:AlignmentsMolecularGenBank","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135990421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oceanographic studies as the basis for shipyard development at the Fish Landing Port of Sabang 海洋学研究是沙邦鱼港船厂发展的基础
Depik Jurnal Pub Date : 2023-08-31 DOI: 10.13170/depik.12.2.33365
Thaib Rizwan, Rianjuanda Djamani, Ilham Zulfahmi, Ayana Rizki, Razali Thaib, Akhyar Akhyar, Muhammad Muhammad, Rahmat Rizqi
{"title":"Oceanographic studies as the basis for shipyard development at the Fish Landing Port of Sabang","authors":"Thaib Rizwan, Rianjuanda Djamani, Ilham Zulfahmi, Ayana Rizki, Razali Thaib, Akhyar Akhyar, Muhammad Muhammad, Rahmat Rizqi","doi":"10.13170/depik.12.2.33365","DOIUrl":"https://doi.org/10.13170/depik.12.2.33365","url":null,"abstract":"Shipbuilding is one of the marine industries which in development needs to pay attention to various parameters from the sea and land. This research was conducted in March to May 2022 with the aim of identifying the influence of oceanographic factors and water quality on the development of the shipbuilding industry at the fishing port of Sabang City. The oceanographic data used includes tides, wind, bathymetry, and sediment composition observed at 12 station points. The depth and tide data are then processed using QGIS. Wind data was analyzed using the Wind Rose Plot and sediment using the Wentworth scale. Oceanographic factors in Sabang Bay have shown very satisfactory results for the development of fish landing bases. The wind in the waters of Sabang Bay blows northwest with high speeds ranging from ≥10.00 m/s. The depth of the waters indicates a potential location for ship mooring. Sandy sediment is the most common type of sediment found. The tides that occur in the bay of Sabang are relatively stable. Oceanographic factors in Sabang Bay have shown very satisfactory results for the development of fish landing bases.Keywords:OceanographyShipyardSabang Bay","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135990419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The abundance and types of plankton in milkfish ponds at Banyuasin South Sumatera 南苏门答腊Banyuasin遮目鱼池塘中浮游生物的丰度和种类
Depik Jurnal Pub Date : 2023-08-31 DOI: 10.13170/depik.12.2.30065
Mirna Fitrani, Reni Septiyani, Dade Jubaedah, Marini Wijayanti, Annisa Septimesy, Yenni Sri Mulyani
{"title":"The abundance and types of plankton in milkfish ponds at Banyuasin South Sumatera","authors":"Mirna Fitrani, Reni Septiyani, Dade Jubaedah, Marini Wijayanti, Annisa Septimesy, Yenni Sri Mulyani","doi":"10.13170/depik.12.2.30065","DOIUrl":"https://doi.org/10.13170/depik.12.2.30065","url":null,"abstract":"Tidal lowland is an area with significant potential for fisheries development, especially in aquaculture. Abundant water supply and land availability provide great opportunities for cultivating aquaculture commodities such as fish, shrimp, and crab. However, effective water quality management poses a significant challenge, necessitating a comprehensive evaluation based on biological, physical, and chemical assessment. Among the crucial parameters influencing water fertility, the presence of plankton stands out. Therefore, this study aimed to investigate water fertility by identifying plankton species, abundance, diversity, dominancy, and water quality in milkfish ponds at Banyuasin, South Sumatra. The results showed that the abundance of phytoplankton and zooplankton ranged from 1030-1791 individual L-1 and 103-461 individual L-1, respectively. Furthermore, Bacillariaceae was recorded to be the highest specie, followed by Cyanophyta, Chlorophyta, Protozoa, Rotifer, and Arthropoda. The range diversity index varied between 1 to 2.6, while the density and dominance indices ranged from 0.9 and 1, as well as 0.1 and 0.8, respectively. The classification of the pond's fertility fell within the mesotrophic category, signifying a moderate level. In conclusion, the water quality maintained an optimum range, thereby supporting the growth of plankton and milkfish. Meanwhile, only ammonia was below the optimum range stipulated by PP No. 22 of 2021 suggested.Keywords:bioindicatormilkfishplanktontidal lowlandwater quality","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135990420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The assessment of status of Sibam River and Air Hitam River Pekanbaru city Riau Province using pollution index 利用污染指数评价廖内省北干巴鲁市西巴姆河和空气希塔姆河的状况
Depik Jurnal Pub Date : 2023-08-31 DOI: 10.13170/depik.12.2.29001
Yuliati Yuliati, Eni Sumiarsih, Efawani Efawani, Isma Mulyani, Nur El Fajri
{"title":"The assessment of status of Sibam River and Air Hitam River Pekanbaru city Riau Province using pollution index","authors":"Yuliati Yuliati, Eni Sumiarsih, Efawani Efawani, Isma Mulyani, Nur El Fajri","doi":"10.13170/depik.12.2.29001","DOIUrl":"https://doi.org/10.13170/depik.12.2.29001","url":null,"abstract":"The quality of the Siak River is gradually deteriorating with the rapid socio-economic development in its tributary watersheds. S. Sibam and Air Hitam are part of the Siak tributaries in the Siak tributary sub-region in Pekanbaru City. The quality of the Sibam and Air Hitam tributaries in the Siak Watershed of Pekanbaru City has been studied to determine the relative pollution level of water quality standards. Water quality measurements were carried out from January to August 2022 at the upstream, middle, and confluence with the Siak River. The pollution level of the Sibam River and Air Hitam River was measured using the pollution index method. The quality of the Sibam River and Air Hitam waters is compared to the quality standards according to The Regulation of Government of the Republic of Indonesia (RGRI) Number 22/2021 Class III. The water quality parameter measured consisted of dissolved oxygen, TSS, pH, BOD, COD, nitrate, nitrite, ammonia, and total phosphate. Pollution index calculations for the Sibam and Air Hitam rivers show light pollution, with index values ranging from 1.75 to 3.57. Nitrite and ammonia concentrations in the Sibam River do not meet quality standards. Whereas in the Air Hitam River, the BOD, nitrite, and ammonia level exceeds the quality standards (RGRI Number 22/2021 Class III). The highest pollution index is in the middle of the river, with settlements on both rivers.Keywords:Pollution IndexWater QualitySibam RiverAir Hitam River","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135990222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamics of mangrove species dominant area changes in Timbulsloko and Bedono, Demak Timbulsloko和Bedono红树林物种优势区动态变化
Depik Jurnal Pub Date : 2023-08-31 DOI: 10.13170/depik.12.2.32538
Zahra Safira Aulia, Rudhi Pribadi, Muhammad Helmi
{"title":"Dynamics of mangrove species dominant area changes in Timbulsloko and Bedono, Demak","authors":"Zahra Safira Aulia, Rudhi Pribadi, Muhammad Helmi","doi":"10.13170/depik.12.2.32538","DOIUrl":"https://doi.org/10.13170/depik.12.2.32538","url":null,"abstract":"Mangrove forests in Timbulsloko and Bedono villages have very important benefits in minimizing abrasion, due to tidal flooding and land absorption in this area. A large number of people have planted mangroves to restore the function of mangrove forests in coastal areas which conducted by NGOs, students, government agencies, and awareness from local residents. This study aimed to determine the dynamics of mangrove area and mangrove species dominant area changes in 2016, 2018, 2020 and 2022 based on Sentinel-2 Satellite Imagery processing, and to analyze the dynamic changes based on geospatial analysis. The method used in this study is divided into two: satellite imagery data processing and field survey. The result showed that the area of mangrove species in Timbulsloko and Bedono increased from 2016 to 2022. In 2016 the area of mangroves was 140.04 ha, 159.57 ha in 2018, 171.05 ha in 2020, and 234.8 ha in 2022. The use of Sentinel 2 Satellite Imagery to map the distribution of mangrove species dominant produce overall accuracy of 84.62%. The mangrove species with the highest area are Avicennia marina followed by Avicennia alba, Rhizophora apiculata, and Rhizophora mucronate. The increase in this area of mangroves in this area is due to natural additions and artificial additions due to mangrove planting conservation by several parties and the awareness of the local residents to protect mangroves.Keywords:Mangrove SpeciesDynamicsCoastal AreaSentinel 2Remote Sensing","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"383 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135990223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of the effect of processing techniques on the quality of galantine made from milkfish (Chanos chanos) 加工工艺对遮目鱼galantine品质影响的研究
Depik Jurnal Pub Date : 2023-08-31 DOI: 10.13170/depik.12.2.33016
Pola Sabar Tumohom Panjaitan, Iman Mukhaimin, Susi Ratnaningtyas, Liliek Soeprijadi, Devi Wulansari
{"title":"Study of the effect of processing techniques on the quality of galantine made from milkfish (Chanos chanos)","authors":"Pola Sabar Tumohom Panjaitan, Iman Mukhaimin, Susi Ratnaningtyas, Liliek Soeprijadi, Devi Wulansari","doi":"10.13170/depik.12.2.33016","DOIUrl":"https://doi.org/10.13170/depik.12.2.33016","url":null,"abstract":"Milkfish (Chanos chanos) is one of the commonly consumed fishery commodities in Indonesia. However, diversification of processed products from milkfish, such as pindang (fish brine) and soft bone milkfish, is considered too simple. Whereas, milkfish has a high nutritional content. An innovation to attract more people to consume this highly nutritious commodity is to process it into fish galantine. Milkfish galantine is processed with the basic ingredients of milkfish, bread flour, and eggs that are seasoned then molded and processed at high temperatures. The process of making milkfish galantine includes preparing milkfish as raw materials, scaling and gutting the fish, mincing the milkfish meat, mixing it with other ingredients, molding, and processing. Fish galantine is generally made through high-temperature processing, such as roasting and steaming. Different high-temperature processing techniques can affect the quality of fish galantine. Therefore, this research aimed to assess the effect of milkfish composition and different high-temperature processing techniques: roasting and steaming. The different milkfish galantine formulations in this research were made by giving different proportions of minced milkfish meat as treatments: 5%, 10%, and 15%. After that, galantines were processed by steaming. Furthermore, the formulation with the highest hedonic scale score was duplicated by processing using the roasting technique. The results of the four treatments were then tested for proximate composition, vitamins, and minerals. The results of the hedonic evaluation of milkfish galantines processed by steaming showed that milkfish galantine with 15% fish meat proportion had the highest score. The test results showed that the addition of milkfish meat caused significant differences in water, ash, total fat, carbohydrate, protein, and potassium contents. In addition, the processing technique also caused significant differences in all parameters tested.Keywords:MilkfishFish galantineSteamingRoasting","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135990422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Important value index (IVI) and diversity of mangrove vegetation in Aceh Tamiang, Aceh Province 亚齐省亚齐Tamiang红树林植被重要价值指数(IVI)与多样性
Depik Jurnal Pub Date : 2023-08-28 DOI: 10.13170/depik.12.2.31130
Irma Dewiyanti, Muhammad Rifki, Chitra Octavina, Maria Ulfa, Adrian Damora, Nurfadillah Nurfadillah
{"title":"Important value index (IVI) and diversity of mangrove vegetation in Aceh Tamiang, Aceh Province","authors":"Irma Dewiyanti, Muhammad Rifki, Chitra Octavina, Maria Ulfa, Adrian Damora, Nurfadillah Nurfadillah","doi":"10.13170/depik.12.2.31130","DOIUrl":"https://doi.org/10.13170/depik.12.2.31130","url":null,"abstract":"Mangrove ecosystem is one of the unique ecosystems that has high productivity and it grows along the coast of Aceh Province, Indonesia. The mangrove vegetation in Aceh Tamiang District was decreasing due to human exploitation i.e. stems of mangrove vegetation utilized by local communities become charcoal and firewood. These activities have a negative impact on live and growth of mangrove vegetation. Therefore, the purposes of the study are to analyses the important value index (IVI), mangrove species diversity, and to analyses the correlation between substrate characteristics with mangrove density. The research stations determined using the purposive sampling method and each station has three plots as replication. There were 4 research stations located in Seruway, Bandahara, Banda Mulia, and Manyak Paye sub districts. The study reported 12 species of mangrove in the tree’s category, 9 species of saplings, and 8 species of seedlings. The 12 species of mangroves found were Avicennia lanata, A. marina, A. officinalis, Brugeira parviflora, B. sexangula, Ceriops decandra, C. tegal, Lumnitzera recomosa, Nypa fruticans, Rhizopora apiculata, R. stylosa, and Sonneratia alba. The R. apiculata was species commonly found in each sampling location and it had highest IVI for the tree, sapling and seedling categories with the value of 207.24, 300, and 200, respectively. The average of diversity index was 1.56 categorized in the medium category. The moment Pearson correlation showed there was no significant correlation between the physical-chemical water parameters and substrate with the density of mangrove where Psig0.05.Keywords:Mangrove vegetationImportant value indexDensityDiversityAceh Tamiang","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135134016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comparative analysis of osteocranium morphology in Lutjanus johnii (Bloch, 1792) and Lutjanus gibbus (Forsskal, 1775) inhabiting different habitats 不同生境下johnlutjanus (Bloch, 1792)和Lutjanus gibbus (Forsskal, 1775)骨颅骨形态的比较分析
Depik Jurnal Pub Date : 2023-08-28 DOI: 10.13170/depik.12.2.32914
Muhammad Radhi, Firdus Firdus, Zainal Abidin Muchlisin
{"title":"A comparative analysis of osteocranium morphology in Lutjanus johnii (Bloch, 1792) and Lutjanus gibbus (Forsskal, 1775) inhabiting different habitats","authors":"Muhammad Radhi, Firdus Firdus, Zainal Abidin Muchlisin","doi":"10.13170/depik.12.2.32914","DOIUrl":"https://doi.org/10.13170/depik.12.2.32914","url":null,"abstract":"The snapper species Lutjanus gibbus (L. gibbus) is commonly found in coastal areas characterized by coral reefs and clear water, while Lutjanus johnii (L. johnii) resides in mangrove waters with murky conditions and strong currents. The habitat plays a crucial role in influencing body shape and ossification patterns in fish. Therefore, this study aimed to examine the differences in the cranial bone morphology between the two Lutjanus fish species that resided in contrasting habitats. The fish samples were collected from local fishermen operating in the waters of Pulo Aceh, Aceh Besar, Indonesia. Following capture, the fish were transported and landed at Lampulo Fishing Port in Banda Aceh for further analysis. For each species, a total of 5 fish samples were randomly selected, and thorough cleaning of scales and muscles was performed before the drying of the bones. These dried cranial bones were then photographed, edited, and subjected to detailed analysis. The study showed that, of the 23 observed skull bone characters, 15 characters showed the differences in the cranial bones of the two snapper species, including the pre-ethmoidal, ethmoidal lateralis, prefrontal, supraorbital, nasal, ethmoidal, sphenoticum, crista occipital, lacrimal, inter-opercular, opercular, preopercular premaxilla, endopterygoideum, and hyomandibular. Significant differences were observed in the bones composing the eyes, nose, jaws, and ventral region of the head. In the mangrove snapper (L. johnii), the bones associated with the eyes (orbitospenoidal, supraorbital, and prefrontal) were less developed, while those related to the nose (nasal, ethmoidal, ethmoidal lateralis, pre-ethmoidal) showed well-developed characteristics. The coral snapper L. gibbus displayed a relatively small but thicker premaxilla bone in the jaw region, along with smaller bones in the ventral head area.Keywords:OsteologyMorphologyEcomorphologyOsteocraniumMaxillare","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135134015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments 红树林沉积物根际细菌宏基因组分析的有效DNA提取方法
Depik Jurnal Pub Date : 2023-08-28 DOI: 10.13170/depik.12.2.32850
Siti Maysaroh, Meutia Samira Ismet, Beginer Subhan, Rita Andini, Enny Rimita Sembiring, Nurlita Putri Anggraini
{"title":"Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments","authors":"Siti Maysaroh, Meutia Samira Ismet, Beginer Subhan, Rita Andini, Enny Rimita Sembiring, Nurlita Putri Anggraini","doi":"10.13170/depik.12.2.32850","DOIUrl":"https://doi.org/10.13170/depik.12.2.32850","url":null,"abstract":"Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135134017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信