Depik Jurnal最新文献

{"title":"Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province","authors":"I. Dewiyanti, D. Darmawi, Z. Muchlisin, T. Z. Helmi, I. I. Arisa, C. N. Defira, F. Fitriyani, Sawva Yura","doi":"10.13170/depik.10.3.22964","DOIUrl":"https://doi.org/10.13170/depik.10.3.22964","url":null,"abstract":"Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian //  TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88901013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal contamination of Hg and Pb in water, sediment and Violet Batissa (Batissa violacea Lamark, 1818) meat in Teunom River, Aceh Jaya Regency, Indonesia","authors":"F. Fauziah, A. Abdullah, S. Supriatno, F. Firdus, M. Nasir, S. Mellisa, Agung Setia Batubara","doi":"10.13170/depik.10.3.23432","DOIUrl":"https://doi.org/10.13170/depik.10.3.23432","url":null,"abstract":"Violet Batissa (Batissa violacea) is an important economic organism on the west-south coast of Aceh, because it is relatively high consumed by the community. However, gold mining activities carried out around the watershed are estimated to pollute the river and cause B. violacea to also be affected. The purpose of this study was to determine the level of mercury (Hg) and Lead (Pb) contamination in clam meat in Teunom, Aceh Jaya Regency. The research was conducted from June to September 2021. Sampling of clam was done by purposive sampling method. Hg and Pb were analyzed in clam meat using the Atomic Absorption Spectrophotometer (AAS) method. The results of the analysis showed that the Hg content in clam meat was between 0.12-0.63 mg/kg (mean 0.35±0.26 mg/kg), water 0.0026-0.0103 mg/kg (mean 0.0052±0.004 mg/kg), and sediment 1.3224-3.8767 mg/kg (mean 2.2324±1.427 mg/kg). Furthermore, the results of the analysis showed that the Pb content in clam meat, water and sediment had the same value at 3 stations with values 0.0002±0 mg/kg, 0.0003±0 mg/kg, and 0.0002±0 mg/kg, respectively. The conclusion of this study is that the Hg content in water and sediment has exceeded the threshold, while the clam meat is still in good quality standards. The Pb content in clam meat, water and sediment were also in good quality standards. Based on our study, B. violacea is still safe for consumption, while the water has been polluted and is recommended not to be utilized for human consumption.Keywords:Heavy metalRiverWaterSedimentBatissa violacea","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"276 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73231484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of fish silage to increase feed efficiency and growth of grouper (Epinephelus coioides) in floating net cages","authors":"A. As, M. Amin, B. Baihaqi, M. Hatta, E. Ayuzar","doi":"10.13170/depik.10.3.23105","DOIUrl":"https://doi.org/10.13170/depik.10.3.23105","url":null,"abstract":"This study aimed to investigate the efficiency of fish silage mixed in compounded diets on growth and survival rate of grouper (Epinephelus coioides). Grouper (2,34±0,24 cm, and 4,17±1,82 g) were divided into four groups and cultivated in 1x1x1,5 m floating net cages. Each group fed with compounded diets containing 0, 1, 3 and 5 g/kg fish silage diet twice daily. Fish were sampled for growth performances and feed efficiency at 14 days interval for 10 weeks. Results indicated that compounded diets at 1 and 3 g/kg silage affected the growth rate. Total length, specific growth rate and weight gain significantly increased in fish received 1 and 3 g/kg silage diets. The feed intake seen to be highest in groups administered 3 g/kg silage, whereas the best feed conversion ratio was found in fish fed 1 g/kg silage. Thus, this study indicated that using fish silage in compounded diets may affect feed efficiency and growth performances of grouper juveniles.Keywords:Artificial dietFeedingFish silageGrouperNet cages","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86118416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of integrated multi-trophic aquaculture (IMTA) using floating net cages on Tilapia fish with native fish (Peres, Lemeduk, and Depik)","authors":"N. Nurfadillah, I. Hasri, Muhammad Reza Purnama, A. Damora, S. Mellisa","doi":"10.13170/DEPIK.10.3.22465","DOIUrl":"https://doi.org/10.13170/DEPIK.10.3.22465","url":null,"abstract":"Environmental problems due to aquaculture occur along with the increase in aquaculture production. IMTA is one system used as a solution in dealing with environmental problems. The objective of this study was to analyze the growth performance, survival rate, and production of the main commodity (tilapia) and the supporting commodities (Peres, Lemeduk, and Depik) which are applied through the application of integrated multi-trophic aquaculture (IMTA) using floating net cages. This research was conducted at the Lukup Badak Fish Seed Center, Central Aceh from July to August 2020. The experimental design used was a completely randomized design (CRD) with 4 treatments and 3 replications, namely: A (tilapia), B (tilapia and peres), C (tilapia and lemeduk), and D (tilapia and depik). ANOVA test results showed that the IMTA system had a significant effect on absolute length growth, absolute weight growth, specific growth rate (SGR), survival rate, and biomass production of tilapia (P0.05). The results showed that maintenance for 42 days produced the highest value in treatment B with the increase in absolute length growth (4.26cm±0.24); absolute weight growth (5.47g±0.45), specific daily growth rate (2.28%/day±0.13) and the highest tilapia biomass production (480g/0.5m2±19.25). The highest survival rate was found in treatment C (82.42%±2.28). It was concluded that the treatment using the IMTA system was better than without the IMTA system.Keywords:IMTAWater qualityGrowth performanceTrophic interactionBiomass production","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88122285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data accuracy in capture fisheries production at Cilacap Ocean Fishing Port","authors":"Ernani Lubis, A. Pane, Putri Nirwana Paramita","doi":"10.13170/DEPIK.10.3.17040","DOIUrl":"https://doi.org/10.13170/DEPIK.10.3.17040","url":null,"abstract":"Accurate capture fishery production data at the fishing ports is crucial, especially for planning the development of capture fisheries and fishing ports. This research aims to analyze the mechanism and accuracy in collecting capture fishery data at the fishing port, especially Cilacap Ocean Fishing Port (OFP). The data collection mechanism of capture fishery production is analyzed through a qualitative descriptive method, while the accuracy of the data was analyzed with a comparative quantitative descriptive method. The results showed three steps in the data collection mechanism at Cilacap OFP: data collection, data recapitulation, and data reporting. The values of deviation and accuracy level of the data on capture fishery were 3,4%-62,1% and 37,9%-96,6%, respectively. This result concluded that the data recorded by the enumerator were more accurate than those in the logbook classified as inaccurate.Keywords:CilacapDataFishAccurateFishing Port","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80363980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of research methodology on the content of heavy metals in sediments on the Indonesian coastal","authors":"S. A. Azis, M. Irham, S. Sugianto, I. Setiawan, A. Adnan","doi":"10.13170/DEPIK.10.3.21275","DOIUrl":"https://doi.org/10.13170/DEPIK.10.3.21275","url":null,"abstract":"Heavy metal pollution is one of the problems that often occurs in Indonesian coastal waters. Comparative methods of looking at heavy metal content in coastal sediments include a way to show similarities and differences between one or more articles by using certain criteria. Different comparison methods to see the heavy metal content in sediments include the AAS (Atomic Absorption Spectrophotometer) method, AAN (Netron Activation Analysis), Regression, and USEPA methods. Based on the comparison of 2 methods, namely: AAS and AAN methods, the most efficient in analyzing heavy metals in sediments in coastal areas is the Atomic Absorption Spectrophotometry (AAS) method compared to the Neutron Activation Analysis (AAS) method.Keywords:CoastalSedimentHeavy MetalSpectrophotometer","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83247726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of NPK fertilizer with different dosage on the growth rate seaweed (Caulerpa racemosa)","authors":"R. Subur, M. Irfan, N. Akbar","doi":"10.13170/DEPIK.10.3.20848","DOIUrl":"https://doi.org/10.13170/DEPIK.10.3.20848","url":null,"abstract":"NPK is a type of fertilizer that plays a role in increasing growth and survival in plants such as seaweed. This study aims to determine the effect of different NPK fertilizer doses on the growth rate of seaweed (Caulerpa racemosa), and to determine which NPK fertilizer dosage has the best effect on the growth rate of C. racemosa. Research begins with collecting samples of seaweed in coastal waters. Ternate Island District Kastela, and the cultivation process is carried out on Jalan Jan, Tabona Village, South Ternate City. The time of the research was two months from August to October, 2020. This study used 12 units of cool box in the form of cork with a size of 90 x 30 cm, which is used as a container for maintaining of C. racemosa. In each treatment using a seed weight of 50 grams. The NPK fertilizer dosage treatment tested was 4 doses, with 3 replications, namely: treatment A: 40 ml NPK fertilizer; B: 60 ml NPK fertilizer; C: 80 ml NPK fertilizer; D: 0 ml NPK fertilizer (control). Research containers using random placement. The design used was a completely randomized design (CRD), using analysis of variance (ANOVA). The results obtained showed that NPK fertilizer with different doses the effect is not significantly different on the growth rate of C. racemosa with the highest average growth rate in treatment C amounting to 8.725%, followed by treatment B of 8.178%, treatment A of 7.761%, and the lowest was treatment D of 6.519%.Keywords:NPKSeaweedCaulerpa racemosaGrowth rate","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79371541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population structure of Lingula (Bruguière, 1791) in Alue Naga waters, Banda Aceh City, Indonesia","authors":"C. Octavina, M. Ulfah, S. Agustina, H. Haridhi, Ade Yudistira","doi":"10.13170/DEPIK.10.3.20348","DOIUrl":"https://doi.org/10.13170/DEPIK.10.3.20348","url":null,"abstract":"Lingula sp. is one of the genera of the Brachiopoda phylum that lives in the intertidal zone or areas that are affected by tides. The purpose of this study was to determine the population structure of Lingula sp. through the approach of population structure, density, distribution patterns, length and weight relationship and with physical and chemical parameters in the waters of Alue Naga, Syiah Kuala District, Banda Aceh City. The method used in this research is a purposive random sampling method. The results show that the growth pattern of Lingula sp. is classified as negative allometric at three research sites in Alue Naga waters, based on the value of b 3. The density of Lingula sp. was the highest at site 1 with a total of 17.7 ind/m2. While the density of Lingula sp. was the lowest at site 3 with a total of 9.7 ind/m2. The distribution of Lingula sp. at the three sites were uniform, with morisita index (Id) values of 0.352, 0.257, and 0.208 for sites 1, 2, and 3 respectively. In addition, the environmental factors of the three research sites in Alue Naga waters are within the normal limits of life for Lingula sp.Keywords: Alue NagaLingula sp. Population structure","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82956867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the addition of starry triggerfish (Abalistes stellaris) bone charcoal as a feed supplement to the growth performance and intestinal villi length of nile tilapia (Oreochromis niloticus)","authors":"Azwar Thaib, L. Handayani, A. Hanum, N. Nurhayati, F. Syahputra","doi":"10.13170/depik.10.2.20367","DOIUrl":"https://doi.org/10.13170/depik.10.2.20367","url":null,"abstract":"Nile tilapia is a freshwater fish that is widely cultivated in Indonesia. During its development, tilapia aquaculture has constraints such as low growth rates. Therefore, it is necessary to add a feed additive to the feed in the form of charcoal. Charcoal is an adsorbent that has high absorption so that the addition of charcoal in the feed can increase the absorption of nutrients of tilapia. A 45-days trial was conducted to determine the percentage of addition of triggerfish bone charcoal (Abalistes stellaris) as a feed supplement that affect the growth performance and intestines of tilapia. The research design was a non-factorial Completely Randomized Design (CRD) with 4 treatments and 2 replications as treatments including: treatment A (control), B (addition of 1%charcoal), C (addition of 2% charcoal) and D (addition of 3% charcoal). A total of 240 tilapia fingerlings (5-7 cm) were used and placed in 8 aquariums then filled with 72 liters of water. Providing adlibitum (unlimited) feed with a frequency of 2 times a day. The ANOVA test showed that the addition of triggerfish bone charcoal (A. stellaris) in the feed was not significantly different (P 0.05) in weight growth, absolute length and daily length growth but it was significantly different (P 0.05) in feed conversion ratio and the efficiency of feed utilization. Based on result, it was concluded that the addition of 3% charcoal was the best treatment which gave the lengthiest of villi which was increasing the length of the intestinal villi to 71.11 µm from the initial length of 162.22 µm. easel at 233.33 μm, showed the highest feed conversion ratio value and feed efficiency (1.12 %, 89.6%).Keywords:CharcoalFish boneIntestinal histologyStarry triggerfish","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"354 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74132518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grouper DNA barcoding studies in Indonesia: A short review","authors":"N. M. Razi, Z. Muchlisin, S. Maulida, M. Ramadhaniaty, F. Nur, A. Damora, Sumarni Laila Buang Manalu, N. Fadli","doi":"10.13170/depik.10.2.21255","DOIUrl":"https://doi.org/10.13170/depik.10.2.21255","url":null,"abstract":"Indonesia is recognized as one of the territories that have the highest reef fish biodiversity in the world. One of the commercially valuable fish in this area is the groupers (locally name \"kerapu\"). At least 76 grouper species have been reported in Indonesian waters, with three species were categorized into \"vulnerable\", five species \"Data Deficient\", and 68 species under the \"Least Concern\" category based on IUCN classification. The increasing exploitations rate had been reported caused the grouper stocks in Indonesia to decrease and threatened extinction. However, only limited scientific data is available regarding the grouper in Indonesia, including their identification. In most fish landing sites across Indonesia, the groupers are morphologically identified and recorded as \"kerapu\" to replace their scientific species names. Accurate species identification is essential in designing appropriate and sustainable management of fisheries resources. One of the tools that have been used in fish identification is DNA barcoding. In the last two decades, this molecular method has been applied to identify many fish groups globally, including grouper fish. This study reviewed the DNA barcoding approach in grouper identification in Indonesia based on the available literature.Keywords:DNA barcodingGrouperMolecular TaxonomyFisheriesIndonesia ","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79809479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}