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Total plate count and Salmonella spp. in de-boned milkfish (Chanos chanos) in Palu City, Indonesia 印度尼西亚帕卢市去骨遮目鱼(查诺斯查诺斯)的盘子总数和沙门氏菌
Depik Jurnal Pub Date : 2023-08-16 DOI: 10.13170/depik.12.2.30959
Samliok Ndobe, Ellen Oktanike Merpati, Rusaini Rusaini, Novalina Serdiati, Rusdi Rusdi
{"title":"Total plate count and Salmonella spp. in de-boned milkfish (Chanos chanos) in Palu City, Indonesia","authors":"Samliok Ndobe, Ellen Oktanike Merpati, Rusaini Rusaini, Novalina Serdiati, Rusdi Rusdi","doi":"10.13170/depik.12.2.30959","DOIUrl":"https://doi.org/10.13170/depik.12.2.30959","url":null,"abstract":"High total plate count (TPC) and the presence of Salmonella spp. in food products can cause health problems for consumers. De-boned milkfish products are popular with consumers in Palu City, Central Sulawesi, Indonesia, but there is a lack of data on their safety. Therefore, this study aimed to investigate TPC levels and detect contamination by Salmonella spp. in these products. Samples of fresh and processed milkfish were collected from two de-boned milkfish processing sites: the Technical Implementation Unit for the Application of Fishery Product Quality Control (TIU-AQFP) and the Melona Micro, Small and Medium Enterprise (MSME) Group in Palu City. Microbiological assays included counting the number of bacterial colonies (TPC) as well as the isolation and identification of Salmonella spp. through biochemical tests. The study applied a completely randomized factorial design with three replicates per site and per product (12 experimental units). De-boning had a significant (P0.05) effect on TPC (1.26×103 to 2.20×103 CFU/g for de-boned milkfish compared to 4.28×103 to 2.94×104 CFU/g for fresh unprocessed milkfish). However, the types of bacteria identified in fresh and de-boned milkfish, including Klebsiella, Enterobacter and Citrobacter, were present at non-pathogenic levels. No Salmonella spp. contamination was found in the test samples. These results indicate that de-boned milkfish products from the TIU-AQFP and Melona MSME Group in Palu City are safe and suitable for human consumption.Keywords:Bone-free milkfishBacterial contaminationPathogen assayFish processingFood safety","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135023038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The use of maggot (Hermetia illlucens) oil in artificial feeds on the growth performance and survival rate of saline tilapia (Oreochromis niloticus) 人工饲料中添加蛆油对咸化罗非鱼生长性能和成活率的影响
Depik Jurnal Pub Date : 2023-08-14 DOI: 10.13170/depik.12.2.29962
Seto Windarto, Hasbi Ash Shiddieqi, Slamet Budi Prayitno, Damang Suryanto, Sapto Purnomo Putro, Vivi Endar Herawati
{"title":"The use of maggot (Hermetia illlucens) oil in artificial feeds on the growth performance and survival rate of saline tilapia (Oreochromis niloticus)","authors":"Seto Windarto, Hasbi Ash Shiddieqi, Slamet Budi Prayitno, Damang Suryanto, Sapto Purnomo Putro, Vivi Endar Herawati","doi":"10.13170/depik.12.2.29962","DOIUrl":"https://doi.org/10.13170/depik.12.2.29962","url":null,"abstract":"Tilapia is one of the commodities favored by the community and is widely cultivated in Indonesia. At this time, the development of tilapia cultivation has entered the location of marine and brackish waters, known as saline tilapia. The increase in tilapia cultivation has increased feed production, so the price of raw materials, especially fish oil, has increased. The source of crude fat that is usually used in feed formulations is fish oil, but the availability of fish oil is limited, so the price is increasing. One of the alternatives that can replace fish oil is maggot oil. Maggot contains 42-48% lipids. The purpose of this study is to find the effect of giving maggot oil on the growth and survival of saline tilapia. The method used was experimental, with a completely randomized design (CRD) consisting of 4 treatments and three replications. The treatments tested were treatment A (control), B (1.5 ml of maggot oil), C (2 ml of maggot oil), D (2.5 ml of maggot oil)) on 100 grams of feed. The saline tilapia used was 1.4-1.9 grams/head. The fish were reared for 30 days in 15-liter volume containers, ten fish density, and were fed three times a day. The results showed that fish oil substitution using maggot oil (H. illucens) significantly affected FUE, SGR, absolute weight, FCR, and SR of saline tilapia. The best dose of maggot oil was found in treatment B with a dose of 1.5 ml/100 grams of feed capable of producing FUE (88.78±3.80%), SGR (7.98±0.23%/day), absolute weight (41 .17± 4.57gram), FCR (1.13±0.05), and SR (100±0.00%).Keywords:BSFBrackish waterSGRProductionWeight","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135308720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of facilities on fish quality in ports of east waters, North Sumatera (case study in belawan fishing port, North Sumatera) 北苏门答腊东部水域港口设施对鱼类品质的影响(以北苏门答腊belawan渔港为例)
Depik Jurnal Pub Date : 2023-08-14 DOI: 10.13170/depik.12.2.29148
Ratih Purnama Sari, Yaser Krisnafi, Mathius Tiku, Roma Yuli F Hutapea, Suci Asrina Ikhsan, Ratu Sari Mardiah
{"title":"The influence of facilities on fish quality in ports of east waters, North Sumatera (case study in belawan fishing port, North Sumatera)","authors":"Ratih Purnama Sari, Yaser Krisnafi, Mathius Tiku, Roma Yuli F Hutapea, Suci Asrina Ikhsan, Ratu Sari Mardiah","doi":"10.13170/depik.12.2.29148","DOIUrl":"https://doi.org/10.13170/depik.12.2.29148","url":null,"abstract":"One of the problems faced in the development of the marine and fisheries sector nationally is the low quality of the fish capture due to the limited capacity and quantity of available port infrastructure resulting in the decrease in competitiveness. The data in this study were related to fish capture from fishing boats to wholesalers. The data obtained was observation of the location of the vessel, the fish auction place, and the marketer. The types of fish taken were Kurisi (Nemipterus pneumatophores), Biji Nangka (Upeneus mullocensin), Kapas-Kapas (Gerres erythronium), Bawal Putih (Pampus argenteus), Kembung (Rastrelliger spp). Fish examining was done by looking at the organoleptic and pH values. The pH value was obtained by testing the pH meter, while the organoleptic value was obtained by observing the physical condition using a score sheet. To determine the magnitude of the influence of facilities on fish quality, and the correlation between organoleptic values and pH, an analysis using simple linear regression was conducted. The average organoleptic value of fish while on the boat, when distributed, and when in the marketplace were 8,248; 7,024; and 6.6 respectively. Meanwhile, the average pH value was 7.05; 5.51; and 5.15. The quality of fish caught at Class A Fishing Port of Belawan is still of good quality. The pH test showed decline in pH value ever since the fish was brought to the port until they were marketed. The influence of facilities on the fish organoleptic and pH value are 98.3% and 96%. Thus, facilities significantly affect fish quality at Belawan Fishing Port. Between pH and organoleptic values showed a positive correlation, hence when the pH value decreased, the organoleptic value also dropped.Keywords:Belawan Fishing PortEast WatersFacilities InfluenceFish Quality","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135308721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of content and bioconcentration of heavy metal (Pb,Hg, and Cd) in green mussels (Perna viridis) based on different sizes and ages 不同大小和年龄青贻贝(Perna viridis)重金属(Pb、Hg、Cd)含量和生物浓度的研究
Depik Jurnal Pub Date : 2023-08-13 DOI: 10.13170/depik.12.2.29824
Bella Krismonita, Indra Gumay Yudha, Munti Sarida, Qadar Hasani, Endang Linirin Widiastuti
{"title":"Study of content and bioconcentration of heavy metal (Pb,Hg, and Cd) in green mussels (Perna viridis) based on different sizes and ages","authors":"Bella Krismonita, Indra Gumay Yudha, Munti Sarida, Qadar Hasani, Endang Linirin Widiastuti","doi":"10.13170/depik.12.2.29824","DOIUrl":"https://doi.org/10.13170/depik.12.2.29824","url":null,"abstract":"Green mussel (Perna viridis) has filter-feeding habits and a sessile way of life, with the main food components of plankton and organic matter in the waters. These food components can contaminate green mussels when it accumulates heavy metals. Therefore, this study aims to evaluate heavy metals content and the bioconcentration of lead (Pb), mercury (Hg), and cadmium (Cd) in green mussels based on different sizes and ages. The study was conducted on Pasaran Island, Lampung, Indonesia, and used green mussels aged 3, 5, 7 to 9 months as samples, with 2 replications. The AAS (Atomic Absorption Spectrophotometry) method was used in the analysis of Pb, Hg, and Cd, sampling technique used purposive sampling. The result showed that green mussels at different ages and sizes has impact on Pb content, while an increase of one unit of age and size of green mussels has no effect on the metal content of Hg and Cd. Furthermore, the highest bioconcentration of Pb and Hg was obtained at 9 months, while Cd was observed at 3 months. Based on the results of this study it can be concluded that the simple linear regression appropriate a functional relationship between Pb and the different ages and sizes green mussels cultivated on Pasaran Island , while heavy metals Hg and Cd appropriate with a polynomial quadratic approach and almost do not exist in the form of a functional relationship between Hg and Cd with the different ages and sizes green mussels. Even though it contains heavy metals in below the maximum limit, but care needs to be taken in addressing this phenomenon due to its nature heavy metals that can accumulate in living tissue, thus consuming green mussels containing heavy metals even in relatively small amounts is not recommended.Keywords:Filter feederSessileAccumulateOrganic matterContaminate","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135309780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Round scad quality on mini purse seine boats, Rembang Regency, Central of Java 爪哇中部,伦邦摄政,迷你围网船上的圆形鱼片质量
Depik Jurnal Pub Date : 2023-08-13 DOI: 10.13170/depik.12.2.27130
Sri Untari Puji Rejeki, Y.S. Darmanto, Eko Nurcahya Dewi, Dwi Apriliani AGS
{"title":"Round scad quality on mini purse seine boats, Rembang Regency, Central of Java","authors":"Sri Untari Puji Rejeki, Y.S. Darmanto, Eko Nurcahya Dewi, Dwi Apriliani AGS","doi":"10.13170/depik.12.2.27130","DOIUrl":"https://doi.org/10.13170/depik.12.2.27130","url":null,"abstract":"Mini purse seine boats in Rembang are divided into two sizes. The first one, namely mini purse seines measuring 10 - 20 Goss Tonnage (GT) (short trips) with a fishing operation duration of 3 - 7 days. The second one is mini purse seines measuring 21 - 30 GT (long trips) with a fishing operation duration of 7 - 10 days. The dominant catch on this boats is round scad, which used a cooling mechanism with ice blocks and bulking systems. This research evaluates the quality of round scad caught by mini purse seine boats (based on Indonesian National Standard/SNI 2729: 2013) and the quality of ice used in handling the fish (based on SNI 4872: 2015). The sampling technique was purposive sampling. Fish samples were carried out in the first and last hold on the short trips and long trips boats, where each handle of the fish was sampled in the upper, middle, and lower layers. Data analysis for sensory evaluation were analyzed by Kruskal Wallis statistics, while the TPC value of round scad was tested by ANOVA test. The laboratory test results obtained the sensory assessment of round scad. The value of the TPC round scad and the E. coli ice blocks are still within the limits of the Indonesian national standards, but the TPC scale of the ice block exceeds the standard limit of SNI. The Kruskal Wallis statistics results showed tha the difference in fish hold, the difference in the trip, and differences in fish hold layers had a significant effect on the sensory of scad. ANOVA test showed that differences in the fish hold and trip differences had a significant impact on the TPC of scad. In contrast, the difference in fish hold layers gave a non-significant effect on the TPC of round scad.Keywords:Quality organolepticTPcRound scadMini purse seineRembang","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135309779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of herbal probiotics in feed on growth and blood profile of elver eels (Anguilla bicolor McClelland, 1844) 草药益生菌在饲料中的应用对鳗鲡生长和血液特征的影响(Anguilla bicolor McClelland, 1844)
Depik Jurnal Pub Date : 2023-08-13 DOI: 10.13170/depik.12.2.29105
Harun Harun, Muamar Abdan, Suprihadi Suprihadi, Fazril Saputra, Kharunnisak Kharunnisak, Said Samsuli, Rahmat Sulni
{"title":"Application of herbal probiotics in feed on growth and blood profile of elver eels (Anguilla bicolor McClelland, 1844)","authors":"Harun Harun, Muamar Abdan, Suprihadi Suprihadi, Fazril Saputra, Kharunnisak Kharunnisak, Said Samsuli, Rahmat Sulni","doi":"10.13170/depik.12.2.29105","DOIUrl":"https://doi.org/10.13170/depik.12.2.29105","url":null,"abstract":"Shortfin eel cultivation in Indonesia has shown significant development in aquaculture. However, various challenges still hinder its successful implementation. This study aims to investigate the impact of herbal probiotics on the growth and blood profile of elver eels (Anguilla bicolor). The research was conducted at UPR Mina Mandiri in the Beutong District of Nagan Raya Regency, from August to October 2022This research was conducted at UPR Mina Mandiri, Beutong District, Nagan Raya Regency, from August to October 2022. A Completely Randomized Design (CRD) with five treatment levels and three replications was employed for this study. The treatments consisted of herbal probiotics added to the eels' feed: 0, 15, 20, 25, and 30 ml/kg. The data obtained from the experiment were subjected to an analysis of variance (ANOVA) to assess the significance of the results. ANOVA test results show that probiotics significantly affect survival rate, absolute weight growth, specific weight rates, feed efficiency, and the elver eel blood profile (hemoglobin, erythrocytes, and leukocytes). (P0.05). Duncan's advanced test revealed significant differences in the growth parameters and blood profiles among the various treatments. Treatment B, which utilized a dosage of 15 ml/kg of feed, displayed the most favorable outcomes. It achieved a survival rate of 93.33%, absolute weight growth of 2.69 grams, a specific growth rate of 1.73%, and a feed efficiency of 48.56%. Additionally, the blood profile measurements for treatment B were as follows: hemoglobin levels ranged from 9.53 to 9.73 g/dl, erythrocyte count ranged from 1.12 to 1.23 x 103 cells/mm3, and leukocyte count ranged from 120 to 133 x 103 cells/mm3.Keywords:blood profilefish healthgrowthshortfin eels probiotics","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135309778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection 食用咖啡叶提取物对嗜水气单胞菌感染后鲤鱼血液学的改变
Depik Jurnal Pub Date : 2023-07-02 DOI: 10.13170/depik.12.2.28682
Shandy Sulistyoningrum, Gunanti Mahasri, Mohammad Faizal Ulkhaq, Hapsari Kenconojati
{"title":"Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection","authors":"Shandy Sulistyoningrum, Gunanti Mahasri, Mohammad Faizal Ulkhaq, Hapsari Kenconojati","doi":"10.13170/depik.12.2.28682","DOIUrl":"https://doi.org/10.13170/depik.12.2.28682","url":null,"abstract":"Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135801762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of different feed pellet on growth rate and survival levels of tilapia seeds (Oreochromis niloticus) 不同饲料颗粒对罗非鱼种子生长速率和成活水平的影响
Depik Jurnal Pub Date : 2023-06-28 DOI: 10.13170/depik.12.2.29174
Muhammad Ikhsan Wamnebo, Siti Hadijah, Andi Hamdillah
{"title":"Effect of different feed pellet on growth rate and survival levels of tilapia seeds (Oreochromis niloticus)","authors":"Muhammad Ikhsan Wamnebo, Siti Hadijah, Andi Hamdillah","doi":"10.13170/depik.12.2.29174","DOIUrl":"https://doi.org/10.13170/depik.12.2.29174","url":null,"abstract":"Tilapia (Oreochromis niloticus, Linnaeus, 1758) is a dominant commodity in the Indonesian freshwater fishery industry, and its growth relies on feed. Pellet are feed that is processed through a factory mechanism or human intervention, based on the cultivators’ needs. Therefore, the purpose of this study was to determine the effect of pelleted feed with varying protein content on growth and survival of tilapia (Oreochromis niloticus) fry. This was conducted from April 2022 to June 2022, in Tasiwalie Village, Suppa District, Pinrang Regency. The test animals used were tilapia aged 30 days, and feed include omega-3, Hi-Pro-vite 781, and Prima feed 500 grams each. An experimental method was employed using a Completely Randomized Design (CRD) with three treatment and replications, where treatment A, B, and C were Prima Feed (39 – 41%), Hi-Pro Vite (protein 31 – 33%), and Omega feed (protein 13 – 16%), respectively. Artificial feed was given at a dose of 5%/body weight 3 times a day. Also, water quality management was carried out by measuring temperature, dissolved oxygen, pH, and ammonia. The results showed that different feed affected protein retention with the highest average value of 43.23%, the highest average fat retention of 48.56%, absolute weight growth of 1.58 g, absolute length growth of 2.33 cm, daily specific weight growth rate of 3.75%, daily specific length growth rate of 5.56%, and feed utilization efficiency 70.60%. Furthermore, the best growth was obtained in treatment A, involving Prime Feed from 39 – 41%. This study showed that the percentage of protein had a significant effect on growth and survival of tilapia. Therefore, the feed industry is needed to formulate requirements based on tilapia fish, to increase production.Keywords:TilapiaFeedGrowthSurvival","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135359090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytotoxicity of leaves, stems, and flowers of Kecubung (Datura metel) extracts using the Brine Shrimp Lethality Test (BSLT) method 用盐水对虾致死试验(BSLT)方法研究了桂花(曼陀罗)提取物的叶、茎、花的细胞毒性
Depik Jurnal Pub Date : 2023-06-28 DOI: 10.13170/depik.12.2.27445
Rindhira Humairani, Nanda Rizki Purnama, Herpandi Herpandi, Mochamad Syaifudin, Ilham Zulfahmi, Yusrizal Akmal, Muliari Muliari, Agus Putra AS, Agung Setia Batubara
{"title":"Cytotoxicity of leaves, stems, and flowers of Kecubung (Datura metel) extracts using the Brine Shrimp Lethality Test (BSLT) method","authors":"Rindhira Humairani, Nanda Rizki Purnama, Herpandi Herpandi, Mochamad Syaifudin, Ilham Zulfahmi, Yusrizal Akmal, Muliari Muliari, Agus Putra AS, Agung Setia Batubara","doi":"10.13170/depik.12.2.27445","DOIUrl":"https://doi.org/10.13170/depik.12.2.27445","url":null,"abstract":"Brine shrimp (Artemia salina) cytotoxicity assays (BSLT) are one of the commonly used cytotoxic test methods to assess a plant extract's pharmacological activity and toxicity. Thus, this study aimed to examine the toxic levels of Kecubung (Datura metel) leaves extracted using different solvents, namely ethanol and ethyl acetate. The results showed that the LC50 value of flower ethanol, flower ethyl acetate, leaf ethanol, and stem ethyl acetate extracts had an LC50 value 1000, which was included in the toxic category. The LC50 value of flower ethanol is 121.044 ppm, flower ethyl acetate 105.89 ppm, leaf ethanol 639.589 ppm, and stem ethyl acetate 635.276 ppm. Ethanol leaf extract at a concentration of 1000 ppm showed the highest mortality with a percentage of 60% of the total number of A. salina. The flower ethanol extract showed the highest mortality at a concentration of 250 ppm with a percentage of 67% and at a concentration of 1000 ppm with a percentage of 70% of the total number of A. salina. Meanwhile, flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm obtained 100% mortality of A. salina in the first 6 hours. The result shows that the flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm is very toxic compared to other concentrations.Keywords:ToxicityArtemia salinaLC50Ethyl acetateEthanol","PeriodicalId":30450,"journal":{"name":"Depik Jurnal","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135359091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The analysis of chlorophyll-a distribution in fishing areas of Aceh Waters 亚齐水域渔区叶绿素-a分布分析
Depik Jurnal Pub Date : 2023-06-28 DOI: 10.13170/depik.12.2.31405
Muhammad Riandi, Muhammad Irham, Muhammad Rusdi, Anwar Deli, Faisal Abdullah, Edy Miswar
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