The Open Chemical and Biomedical Methods Journal最新文献_第5页

Rapid Flow Cytometric Method for Measuring Mitochondrial Membrane Potential, Respiratory Burst Activity, and Intracellular Thiols of Human Whole Blood Leukocytes 快速流式细胞术测定人全血白细胞的线粒体膜电位、呼吸爆发活动和细胞内硫醇

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-04-01 DOI: 10.2174/1875038900902010065

E. Matteucci, S. Manzini, M. Ghimenti, C. Consani, O. Giampietro

引用次数: 5

SF-ICP-MS Analysis of Palladium in Fluids of Patients Sensitized to Metal-Based Dental Restorations 金属基牙修复体致敏患者体液中钯的SF-ICP-MS分析

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020048

S. Caimi, F. Petrucci, A. Cristaudo, V. Bordignon

{"title":"SF-ICP-MS Analysis of Palladium in Fluids of Patients Sensitized to Metal-Based Dental Restorations","authors":"S. Caimi, F. Petrucci, A. Cristaudo, V. Bordignon","doi":"10.2174/1875038900902020048","DOIUrl":"https://doi.org/10.2174/1875038900902020048","url":null,"abstract":"Palladium (Pd) belongs to the platinum group elements and its use has more than doubled in the past ten years. Encouraged by positive patch test results in some patients suffering from oral syndromes, Pd was included in the routine patch testing for contact dermatitis. In the present study, differences in the trend of sensitization to Pd in a contact derma- titis clinic population between 1996 and 2006 were evaluated. Since many in vitro studies have confirmed the corrosion of dental alloys and indicated that metal components are released in the oral cavity, Pd content was examined in leaching so- lutions from dental prostheses and in samples of saliva, serum and urine of subjects with adverse reaction to dental pros- theses. Measurements were performed by means of Sector Field Inductively Coupled Plasma-Mass Spectrometry (SF- ICP-MS). Results showed that the frequency of mono-reactions to Pd are increased from 1996 (0.11%) to 2006 (1.6%), suggesting that this metal is becoming a \"new\" potential allergen. The mean values of Pd levels detected in urine were higher than those found in saliva and in serum. All biological samples collected from patients showed significantly higher levels of Pd than controls (saliva, 255 ng/L vs 11; serum, 60 vs 15; urine, 580 vs 20). Moreover, after removal of the pros- theses a consistent reduction of Pd content in fluids was observed.","PeriodicalId":302199,"journal":{"name":"The Open Chemical and Biomedical Methods Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130068505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Epidemiology of Contact Allergy to Metals in the General Population: Prevalence and New Evidences 普通人群接触性金属过敏的流行病学:流行程度和新证据

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020026

B. Bocca, G. Forte

引用次数: 16

Diagnostic Methods for Contact Allergy to Metals 金属接触过敏诊断方法

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020058

A. Cristaudo, V. Bordignon

{"title":"Diagnostic Methods for Contact Allergy to Metals","authors":"A. Cristaudo, V. Bordignon","doi":"10.2174/1875038900902020058","DOIUrl":"https://doi.org/10.2174/1875038900902020058","url":null,"abstract":"The epicutaneous patch testing is regarded as the best method of diagnosis for allergic contact dermatitis (ACD). Patch tests can be used to confirm a suspected allergic contact dermatitis and either to recommend avoidance of particular products or to recommend alternative products in a particular patient. It is based upon re-exposing the skin of the patient to suspected allergens under controlled conditions. Different test sites and test tapes can be used and different variables such as intrinsic penetration capacity, concentration, exposure time and vehicle can be changed to obtain an op- timal bioavailability of the haptens. The ideal patch test should cause as few adverse reactions as possible, and be repro- ducible and specific. In this paper, the application fields, the advantages and the disadvantages of the patch tests are re- viewed. Other diagnostic methods as the open test, the provocative test, the repeated open application test and the photo- patch test are also discussed. the risk of degradation due to humidity, air or light. Most preparations should be kept in a refrigerator and in the dark; those in diluted liquid preferably in dark bottles. The aller- gens should not be stored vertically, to prevent sedimenta- tion and concentration changes of the allergens. The test preparation in petrolatum, kept in syringes, is applied di- rectly onto the test chamber. Liquid test preparations are ap- plied via a digital pipette to allow exact dosing.","PeriodicalId":302199,"journal":{"name":"The Open Chemical and Biomedical Methods Journal","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127844129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Diet in Nickel Dermatitis 饮食在镍性皮炎中的作用

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020055

F. Petrucci, B. Bocca, G. Forte, S. Caimi, A. Cristaudo

引用次数: 10

The X-Ray and SF-ICP-MS Analysis of Content and Release of Allergenic Metals from Body Piercing 人体穿孔致敏金属的x射线和SF-ICP-MS含量及释放分析

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020035

B. Bocca, S. Caimi, O. Senofonte, F. Petrucci, R. Feliciani, M. Milana, B. D. Berardis, L. Paoletti, G. Forte

{"title":"The X-Ray and SF-ICP-MS Analysis of Content and Release of Allergenic Metals from Body Piercing","authors":"B. Bocca, S. Caimi, O. Senofonte, F. Petrucci, R. Feliciani, M. Milana, B. D. Berardis, L. Paoletti, G. Forte","doi":"10.2174/1875038900902020035","DOIUrl":"https://doi.org/10.2174/1875038900902020035","url":null,"abstract":"Metallic piercing which have lack of resistance to sweat corrosion might be able to release metallic ions in the local tissue producing adverse effects in the human body, including allergic contact dermatitis (ACD). The aim of this study was double: 1) the metallic characterization of piercings and 2) the release of metals (Al, Co, Cr, Cu, Fe, Ir, Mn, Ni, Pd, Pt, Rh, Ti, V and Zn) in artificial sweat to simulate conditions of use. Stainless steel and titanium piercings were sam- pled, and both the official and non official markets were checked. The X-ray microanalysis by Energy-Dispersion Spec- trometry and the Sector Field Inductively Coupled Plasma Mass Spectrometry were the techniques of election for metallic characterization and leaching analysis. The piercings analyzed were alloys of Fe/Cr/Ni or Fe/Mn/Cr. The coloured pierc- ings had a layer of Ti varying from 0.3 to 0.5 μm above the steel core and an external C stratum (0.04 μm). The titanium piercing was pure Ti. Piercings sampled from the official market released less than 0.05 μg/cm 2 /week of metal and thus, most likely, they are allergologically safe. Piercing purchased from the non official market were more susceptible to sweat corrosion promoting the release of considerable amounts of Cr (2.78 μg/cm 2 /week), Fe (26.95μg/cm 2 /week), Mn (51.15 μg/cm 2 /week) and Ni (0.157 μg/cm 2 /week). Iridium, Pd, Pt and Rh were not leached by any of the analyzed piercings.","PeriodicalId":302199,"journal":{"name":"The Open Chemical and Biomedical Methods Journal","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133452024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Quantification of Sensitizing Metals in Tattooing Pigments by SF-ICP-MS Technique SF-ICP-MS技术定量纹身颜料中致敏金属

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020042

G. Forte, F. Petrucci, A. Cristaudo, B. Bocca

{"title":"Quantification of Sensitizing Metals in Tattooing Pigments by SF-ICP-MS Technique","authors":"G. Forte, F. Petrucci, A. Cristaudo, B. Bocca","doi":"10.2174/1875038900902020042","DOIUrl":"https://doi.org/10.2174/1875038900902020042","url":null,"abstract":"Allergic reactions to metals and metal salts used in pigments for tattoos are surprisingly frequent. The objective of this study was to quantify the metal content of tattoo inks using the sector field inductively coupled plasma mass spec- trometry analysis (SF-ICP-MS). The inks were subjected to a robust microwave digestion in a mixture of nitric and fluoridric acids, and hydrogen-peroxide. A total of 13 tattoo inks including various colors, as black, blue, brown, green, red, violet, white and yellow, were examined for the content of Cd, Co, Cr, Hg and Ni. The limits of detection and quanti- fication were as follows (ng/ml): Cd, 0.02 and 0.07; Co, 0.06 and 0.20; Cr, 0.80 and 2.64; Hg, 0.50 and 1.65; and Ni, 0.40 and 1.32. The method was accurate reporting the following mean recoveries (%): Cd, 92; Co, 94; Cr, 96; Hg, 105; and Ni, 103. The precision of the method was calculated as intra-day (%) and inter-day repeatability (%) and results were: Cd, 2.09 and 5.20; Co, 1.58 and 2.67; Cr, 2.07 and 2.99; Hg, 3.88 and 4.55; and Ni, 4.05 and 5.11. All the tested metals were present in the tattoo pigments, but the relative contribution of elements to the tattoo ink compositions was highly variable between samples and even among like-colored pigments. The highest element was Cr in all the pigments (315-4720 ng/g) followed by Ni (37.5-2318 ng/g) and Cd (6.67-1150 ng/g); the lower elements were Co (2.78-125 ng/g) and Hg (<limit of quantification-179 ng/g). Since no rules regulate tattoo inks, this research can be a starting point for chemical safety as- sessment of commercial inks and for proposal of regulating legislation.","PeriodicalId":302199,"journal":{"name":"The Open Chemical and Biomedical Methods Journal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123392608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

MEET THE GUEST EDITOR 认识一下特邀编辑

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-03-27 DOI: 10.2174/1875038900902020064

B. Bocca

{"title":"MEET THE GUEST EDITOR","authors":"B. Bocca","doi":"10.2174/1875038900902020064","DOIUrl":"https://doi.org/10.2174/1875038900902020064","url":null,"abstract":"Dr. Beatrice Bocca is a researcher at the Bioelements and Health Unit of the Istituto Superiore di Sanità (National Institute of Health, ISS), Rome. She gained a degree, with laude, in Chemistry from the University of Rome in 1997 and after joined the ISS as a research chemist working primary in the spectroscopic areas (ICP MS and ICP OES) and separation sciences (HPLC and GC). More specifically, her research focuses on the development and application of analytical methods for the determination of trace elements and trace amounts of elemental species in environmental, biological and food matrices, in the assessment of reference values for elements in ecosystems and humans, and in the preparation of reference materials for quality control in analytical chemistry. She participated in several national and international research programs on the development of new analytical methodologies, on the monitoring and control of element pollution and on the role of metals in diseases. Current research projects promoted by the Italian Ministry of Health and by the European Commission are: 1) Biomonitoring of population: methodological and toxicological aspects of the exposure to neurotoxic metals; 2) Role of neurotoxic chemical elements in neurodegenerative diseases; 3) Predictive value of metals and oxidative stress in colorectal cancer; 4) Assessment of the occupational exposure to precious metals; 5) Evaluation of the amount of nickel in cosmetics and jewellery and tolerability in sensitized persons. She is speaker in courses on the quantification of metals of toxicological interest in food, biological and environmental matrices, and reviewer for some international journals. She is the author of ca. 130 original research publications in international journals and communications at national and international meetings.","PeriodicalId":302199,"journal":{"name":"The Open Chemical and Biomedical Methods Journal","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133200670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Membrane Filter with Oligosaccharide Immobilized by Click Chemistry for Influenza Virus Adsorption Click化学固定化低聚糖膜过滤器吸附流感病毒的研究

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-01-27 DOI: 10.2174/1875038900902010013

Tomohisa Kato, Atsushi Miyagawa, M. Kasuya, K. Hatanaka

引用次数: 6

Simultaneous Quantitative Analysis of EO9 (Apaziquone) and its Conversion Products EO5a and EO9-Cl in Human and Dog Urine by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry 高效液相色谱-电喷雾串联质谱法同时定量分析人和狗尿液中Apaziquone及其转化产物EO5a和EO9- cl

The Open Chemical and Biomedical Methods Journal Pub Date : 2009-01-27 DOI: 10.2174/1875038900902010001

L. Vainchtein, H. Rosing, D. Mirejovsky, V. Huynh, L. Lenaz, J. Schellens, J. Beijnen

{"title":"Simultaneous Quantitative Analysis of EO9 (Apaziquone) and its Conversion Products EO5a and EO9-Cl in Human and Dog Urine by High-Performance Liquid Chromatography Coupled with Electrospray Tandem Mass Spectrometry","authors":"L. Vainchtein, H. Rosing, D. Mirejovsky, V. Huynh, L. Lenaz, J. Schellens, J. Beijnen","doi":"10.2174/1875038900902010001","DOIUrl":"https://doi.org/10.2174/1875038900902010001","url":null,"abstract":"A sensitive and specific LC-MS/MS assay for the quantitative determination of anticancer agent EO9 and its conversion products EO5a and EO9-Cl in human and dog urine is presented. A 20-μL-urine aliquot was spiked with a mixture of deuterated internal standards EO9-d3 and EO5a-d4 and diluted with 180 �L 0.1 M ammonium acetate - methanol (7:3, v/v). Next, 25 μL-volumes were injected onto the HPLC system. Separation was achieved on a 150 � 2.1 mm C18 column using an alkaline eluent (1 mM ammonium hydroxide - methanol (gradient system)). Detection was executed by positive ion electrospray followed by tandem mass spectrometry. The assay quantifies a range from 0.1 �g/mL to 50 � g/mL for EO9, from 0.2 � g/mL to 50 � g/mL for EO5a and 0.1 � g/mL to 4 � g/mL for EO9-Cl using 20 μL of stabilized urine samples. Validation results demonstrate that EO9, EO5a and EO9-Cl concentrations can be accurately and precisely quantified in human and dog urine. This assay is used now to support pre-clinical and clinical pharma- cologic studies with intravesically administered EO9.","PeriodicalId":302199,"journal":{"name":"The Open Chemical and Biomedical Methods Journal","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115056671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0