Development of Membrane Filter with Oligosaccharide Immobilized by Click Chemistry for Influenza Virus Adsorption

Abstract

Membranes with immobilized GM3-type oligosaccharide were prepared and influenza virus adsorption was evaluated. GM3-type oligosaccharide which has azido group in the aglycon was synthesized by saccharide primer method. The azido group was used for combination by click chemistry with alkyne which is introduced to membranes. The posi- tion of the azido group in the aglycon of GM3-type oligosaccharide was either the farthest (12-azidododecyl), or the near- est (2-azidododecyl). The azido group in the farthest position showed higher reactivity with alkyne on the membrane than the one in the nearest the GM3-type oligosaccharide. Moreover, the membrane immobilized GM3-type oligosaccharide showed adsorption ability of influenza virus.