Journal of Life and Bio Sciences Research最新文献_第4页

Morphological, Histological Changes and Acetyl Cholinesterase Activity in Chick Embryos After Exposure to Abamectin Insecticide 阿维菌素对鸡胚形态、组织学及乙酰胆碱酯酶活性的影响

Journal of Life and Bio Sciences Research Pub Date : 2021-11-01 DOI: 10.38094/jlbsr20248

Banan Taha, R. Mohammed

{"title":"Morphological, Histological Changes and Acetyl Cholinesterase Activity in Chick Embryos After Exposure to Abamectin Insecticide","authors":"Banan Taha, R. Mohammed","doi":"10.38094/jlbsr20248","DOIUrl":"https://doi.org/10.38094/jlbsr20248","url":null,"abstract":"Abamectin is a bio-insecticide, derived from the soil bacteria Streptomyces avermitilis. This insecticide is used in public health and agriculture to protect crops. Major adverse impacts of Abamectin are neurological symptoms acting on the peripheral nervous system. The aim of the current study is to reveal the toxic effects of Abamectin on chick embryo Ross 308 including morphological and histological changes and acetyl cholinesterase activity. 120 fresh fertilized eggs were divided into 6 groups; two of them were used as control. After 2 days of incubation, the eggs were injected with 100 µL of Abamectin solution (diluted at concentrations 360, 540, 900 and 1800 ppm) into the yolk sac. The Results showed that the mortality increased significantly in chicks treated with Abamectin, but had a lower weight in comparison to the control groups. Treated chicks started hatching at day 22 but were physically weak with drooping limbs, paralysis and then died after 24 hours of hatching. Some chicks did not normally hatch and needed assistance. They characterized by limb defects, failure retraction of yolk sac with bleeding. Histological examination of the liver showed hepatic cell degeneration, congestion in the central vein, infiltration of inflammatory cells and hepatocytes necrosis. Furthermore, the Acetyl cholinesterase enzyme analysis showed a significant decrease in the enzyme activity which leads to inhibition the activity of the body systems. It is concluded that low and high concentration of Abamectin has adverse impacts on chick embryo by changing some of morphological, histological characteristics and acetyl cholinesterase activity.","PeriodicalId":300980,"journal":{"name":"Journal of Life and Bio Sciences Research","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123567373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm formation by Candida albicans and Candida glabrata isolated from urine specimens of diabetic Iraqi women 伊拉克糖尿病妇女尿液中白色念珠菌和光秃念珠菌的生物膜形成

Journal of Life and Bio Sciences Research Pub Date : 2021-10-18 DOI: 10.38094/jlbsr20246

Enas A. Ibrahim, Rafea Qasim Hamzah

{"title":"Biofilm formation by Candida albicans and Candida glabrata isolated from urine specimens of diabetic Iraqi women","authors":"Enas A. Ibrahim, Rafea Qasim Hamzah","doi":"10.38094/jlbsr20246","DOIUrl":"https://doi.org/10.38094/jlbsr20246","url":null,"abstract":"Biofilms are multicellular communities where microorganisms are grown and form an extracellular matrix that protects the pathogenic microorganisms from the immunity system and antimicrobial agents. This study is aimed to identify Candida spp. isolated from urine specimens by using traditional techniques, germ tube, growth on corn meal agar medium and chromogenic medium then determine the ability of isolates to producing biofilm by tube method (TM) and congo red agar method (CRA). In our study urine specimens were obtained from 174 diabetic females in the period of six months at the Al-Wafa Specialized Center for Diabetes and Endocrinology, Mosul city, Iraq. Out of the total 174 specimens, yeast species were isolated from 56 (32.2 %) specimens. Out of the 56 isolates, 50 isolates were positive for Candida spp., especially C. glabrata which appeared maximum in 30 isolates (60 %) and followed by C. albicans 18 isolates (36%) and C. krusei 2 isolates (4 %). In the TM method for biofilm detection, C. albicans showed 16 isolates (88.9%) positive for biofilm formation followed by 29 isolates (96.7%) of C. glabrata. Furthermore, in the CRA method, all isolates (100%) of C. albicans were negative followed by 27 isolates (90%) of C. glabrata, whereas only 3 isolates (10%) of C. glabrata were positive. We can conclude that TM is the best conventional method and is sensitive to detect biofilm-forming yeast when compared with the CRA method.","PeriodicalId":300980,"journal":{"name":"Journal of Life and Bio Sciences Research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133284683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staphylococcus epidermidis biofilms: Functional molecules; relation to virulence and the host immune response 表皮葡萄球菌生物膜:功能分子;毒力与宿主免疫反应的关系

Journal of Life and Bio Sciences Research Pub Date : 2021-09-27 DOI: 10.38094/jlbsr20243

F. Abdul, I. Raheem, R. Abdulrazaq, Hanan T. Subhi

引用次数: 0

Evaluation of Health and Environmental Safety in Food and Water Production Factories in Duhok Governorate 杜胡克省食品和水生产工厂的健康和环境安全评价

Journal of Life and Bio Sciences Research Pub Date : 2021-09-27 DOI: 10.38094/jlbsr20244

M. Abdulrahman, Kherailah Bebo

{"title":"Evaluation of Health and Environmental Safety in Food and Water Production Factories in Duhok Governorate","authors":"M. Abdulrahman, Kherailah Bebo","doi":"10.38094/jlbsr20244","DOIUrl":"https://doi.org/10.38094/jlbsr20244","url":null,"abstract":"Lack of adequate occupational safety and health strategies is a reason that workplace accidents in food and water production industries remain high, making this sector one of the riskiest operations worldwide. The research was carried out to examine the degree of implementing the health, occupational and environmental safety measures in the selected factories to assess health and safety level and to measurement the noise levels in these industries. A cross-sectional survey was conducted by utilizing structured pre-tested questionnaire and noise level measured at four places: selling, administrative, machine and passage industry places. The study revealed the absence of health and safety policy, and the absence of safety and training departments. The suitable measures in place to combat harmful dust generated by motor traffic were present in 23.3%, and the system with deal with the spillage was current in 43.3% of factories. Wastes segregation was found in 3.3%. Exhaust dilution ventilation system was seen in 83.3% of these factories. Personal protection equipment was not provided to all workers exposed to dust, fumes, or gases. Only 3.3% of factory staffs were aware of fire prevention and safety measures. The sound level at the machine place was above average (above 85 decibels) in all factories.","PeriodicalId":300980,"journal":{"name":"Journal of Life and Bio Sciences Research","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126099960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Asprosin Levels in Women with Polycystic Ovary Syndrome in Duhok City, Kurdistan Region of Iraq 伊拉克库尔德斯坦地区杜霍克市多囊卵巢综合征妇女血清Asprosin水平

Journal of Life and Bio Sciences Research Pub Date : 2021-06-26 DOI: 10.38094/jlbsr2242

Mohammed Ameen, D. Sulaiman, Khalida Hassan Muho

{"title":"Serum Asprosin Levels in Women with Polycystic Ovary Syndrome in Duhok City, Kurdistan Region of Iraq","authors":"Mohammed Ameen, D. Sulaiman, Khalida Hassan Muho","doi":"10.38094/jlbsr2242","DOIUrl":"https://doi.org/10.38094/jlbsr2242","url":null,"abstract":"Asprosin is a novel peptide hormone produced and secreted by white adipose tissues. Asprosin associated with insulin resistance and promotes hepatic glucose production. Previous studies showed that serum asprosin was raised in the general population with polycystic ovary syndrome (PCOS). However, there were studies supporting the opposite. Also, there were studies that showed the highest levels of asprosin was due to insulin resistance, as well as in type 2 diabetes patients. PCOS is one of the metabolic disorders related to insulin resistance. Therefore, the current study aims to evaluate the levels of asprosin in the blood serum of women with PCOS compared to the healthy women who resident in Duhok in the Kurdistan Region of Iraq. A cross-sectional study was conducted from 20th of June, 2020 to 11th of January, 2021 at Obstetrics and Gynecology Hospital and Mazi medical clinics. Serum asprosin level was determined in 75 women with PCOS (18-44 years) and 96 healthy women. SPSS software was utilized for analyzing the study data. The (means ± SD) of demographic parameters (body mass index (BMI) and waist circumference (WC)) in women with PCOS were significantly highest in comparison to healthy women. The biochemical parameters (serum asprosin, fasting blood sugar (FBS), fasting insulin (FI), total cholesterol (TC), and triglyceride (TG)) in women with PCOS also were remarkably higher compared to healthy women with the exception of high-density lipoprotein- cholesterol (HDL-C). The current data show that serum asprosin variance significantly between WC, BMI, FBS, FI, TC, TG and HDL-C. The study confirms that serum asprosin in women with PCOS was higher than in the healthy women. In addition in women with PCOS it was found that serum asprosin was positively correlated with BMI, WC, FBS, FI, HOMA-IR, TC and TG (P<0.05). Except, HDL-C was negatively correlated with serum asprosin (P<0.01).","PeriodicalId":300980,"journal":{"name":"Journal of Life and Bio Sciences Research","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128599661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Bio-interaction of Agrobacterium rhizogenes with Capsicum annuum L. (sweet variety) and Establishment Hairy Roots Cultures 根农杆菌与甜椒的生物相互作用及毛状根培养的建立

Journal of Life and Bio Sciences Research Pub Date : 2020-09-12 DOI: 10.38094/JLBSR1226

Q. Al-Nema

{"title":"Bio-interaction of Agrobacterium rhizogenes with Capsicum annuum L. (sweet variety) and Establishment Hairy Roots Cultures","authors":"Q. Al-Nema","doi":"10.38094/JLBSR1226","DOIUrl":"https://doi.org/10.38094/JLBSR1226","url":null,"abstract":"Genetic transformation using Agrobacterium is one of the techniques used to transfer desired genes to plants. This protocol is considered a short – cut to get transformed plants which could be an alternative method and suitable system compared with the classical methods. This study aimed to investigate the response of C. annuum seedlings (sweet variety) to the formation transformed hairy roots induced by A. rhizogenes strain R1601. Sterilized seeds of Capsicum annuum were inoculated with the Agrobacterium rhizogenes inoculum. The samples were then transferred to the surface of solidified MS medium. Hairy roots were developed at the inoculation sites and were enucleated 1.0-1.5 cm length and placed in 9.0 cm Petri-dishes containing 15 ml of agar solidified MS medium. Agropine test was performed according to the standard method. The inoculated seedlings showed a good response 90%. Transformed hairy roots were established at the injection sites within 10 days and these roots were easily grown on agar-solidified MS medium. The results are confirmed that these roots were transformed roots it in terms of positive agropine detection. The current study concluded that the biological interaction between Agrobacterium rhizogenes strain R1601 and Capsicum anuum L. seedlings, was successful. This study encourages future research to improve this plant by continuing and applying modern technologies to obtain genetically modified plants.","PeriodicalId":300980,"journal":{"name":"Journal of Life and Bio Sciences Research","volume":"252 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133965395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0