Bioelectricity最新文献

{"title":"Determination of the Effects of Transcutaneous Auricular Vagus Nerve Stimulation on the Heart Rate Variability Using a Machine Learning Pipeline.","authors":"Anna Tarasenko,&nbsp;Stefano Guazzotti,&nbsp;Thomas Minot,&nbsp;Mikheil Oganesyan,&nbsp;Nickolai Vysokov","doi":"10.1089/bioe.2021.0033","DOIUrl":"https://doi.org/10.1089/bioe.2021.0033","url":null,"abstract":"<p><strong>Background: </strong>We are all aware of day-to-day healthy stress, but, when sustained for long periods, stress is believed to lead to serious physical and mental health issues.</p><p><strong>Materials and methods: </strong>In this study, we investigated the potential effects of transcutaneous auricular vagus nerve stimulation (taVNS) on stress processing as reflected in the electrocardiogram (ECG)-derived biomarkers of stress adaptability. Stress reflecting biomarkers included a range of heart rate variability metrics: standard deviation of N-N intervals (SDNN), root mean squared of successive differences in heartbeat intervals (RMSSD), low-frequency component, high-frequency component and their ratio (LF, HF, and LF/HF).In addition, we created a machine learning model capable of distinguishing between the stimulated and nonstimulated conditions from the ECG-derive data from various subjects and states. The model consisted of a deep convolutional neural network, which was trained on R-R interval (RRI) data extracted from ECG and time traces of LF, HF, LF/HF, SDNN, and RMSSD.</p><p><strong>Results: </strong>Only LF/HF ratio demonstrated a statistically significant change in response to stimulation. Although the LF/HF ratio is expected to increase during exposure to stress, we have observed that stimulation during exposure to stress counteracts this increase or even reduces the LF/HF ratio. This could be an indication that the vagus nerve stimulation decreases the sympathetic activation during stress inducement.Our Machine Learning model achieved an accuracy of 70% with no significant variations across the three states (baseline, stress, and recovery). However, training an analogous neural network to identify the states (baseline, stress, and recovery) proved to be unsuccessful.</p><p><strong>Conclusion: </strong>Overall, in this study, we showed further evidence of the beneficial effect of taVNS on stress processing. Importantly we have also demonstrated the promising potential of ECG metrics as a biomarker for the development of closed-loop stimulation systems.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10491795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectricity: An Update","authors":"M. Djamgoz, Michael E. Levin","doi":"10.1089/bioe.2022.0024","DOIUrl":"https://doi.org/10.1089/bioe.2022.0024","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77516860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectricity Industry News","authors":"M. Djamgoz","doi":"10.1089/bioe.2022.0027","DOIUrl":"https://doi.org/10.1089/bioe.2022.0027","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81594959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Offsetting Voltage-Dependent Kv1.5 Channel Opening Through Charged Residue Substitutions on Top of the First Transmembrane Segment","authors":"Kenny M. Van Theemsche, Joni G. Heymans, Nikola Z. Popovic, E. Martínez-Morales, D. Snyders, A. Labro","doi":"10.1089/bioe.2022.0005","DOIUrl":"https://doi.org/10.1089/bioe.2022.0005","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84266895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Platelet Electrome: Evidence for a Role in Regulation of Function and Surface Interaction","authors":"M. Hughes, Emily J. Kruchek, J. Gibbins, Oreoluwa V. Griffiths, Bader Abdal, F. Labeed","doi":"10.1089/bioe.2021.0044","DOIUrl":"https://doi.org/10.1089/bioe.2021.0044","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2022-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79939899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voltage-Gated Potassium Channels Beyond the Action Potential.","authors":"Luis A Pardo","doi":"10.1089/bioe.2022.0014","DOIUrl":"https://doi.org/10.1089/bioe.2022.0014","url":null,"abstract":"<p><p>Bioelectricity goes far beyond electrical signaling in the nervous system, but this was initially not obvious for me. This article describes the journey from studying the biophysics of ion channels in classical electrically excitable tissues to focusing on the pathogenic roles of the Kv10.1 potassium channel in cancers.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10133977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9450483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the Membrane Potential to Combat Cancer Progression.","authors":"Ming Yang, William J Brackenbury","doi":"10.1089/bioe.2022.0001","DOIUrl":"10.1089/bioe.2022.0001","url":null,"abstract":"<p><p>Rapid fluctuations in the plasma membrane potential (V_m) provide the basis underlying the action potential waveform in electrically excitable cells; however, a growing body of literature shows that the V_m is also functionally instructive in nonexcitable cells, including cancer cells. Various ion channels play a key role in setting and fine tuning the V_m in cancer and stromal cells within the tumor microenvironment (TME), raising the possibility that the V_m could be targeted therapeutically using ion channel-modulating compounds. Emerging evidence points to the V_m as a viable therapeutic target, given its functional significance in regulating cell cycle progression, migration, invasion, immune infiltration, and pH regulation. Several compounds are now undergoing clinical trials and there is increasing interest in therapeutic manipulation of the V_m via application of pulsed electric fields. The purpose of this article is to update the reader on the significant recent and ongoing progress to elucidate the functional significance of V_m regulation in tumors, to highlight key remaining questions and the prospect of future therapeutic targeting. In particular, we focus on key developments in understanding the functional consequences of V_m alteration on tumor development via the activation of small GTPase (K-Ras and Rac1) signaling, as well as the impact of V_m changes within the heterogeneous TME on immune cell function and cancer progression.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77532728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectricity of the Tumor Microenvironment.","authors":"Paul J Buchanan, Christophe Vandier","doi":"10.1089/bioe.2022.0019","DOIUrl":"10.1089/bioe.2022.0019","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77914875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxic Signaling Is Modulated by Calcium Channel, CaV1.3, in Androgen-Resistant Prostate Cancer.","authors":"Debbie O'Reilly, Paul J Buchanan","doi":"10.1089/bioe.2022.0007","DOIUrl":"10.1089/bioe.2022.0007","url":null,"abstract":"<p><strong>Background: </strong>Androgen deprivation therapy (ADT) remains a key treatment for advance prostate cancer (PCa), but resistance leads to terminal castrate-resistant prostate cancer (CRPC). Its development is linked to the emergence of a hypoxic tumor microenvironment and associated hypoxia inducible factor (HIF) signaling, which is known to be modulated by intracellular calcium. ADT is also known to upregulate store-operated calcium entry (SOCE) through voltage-gated calcium channel, CaV1.3. Consequently, the role of CaV1.3 in supporting hypoxic signaling and CRPC biology was explored.</p><p><strong>Materials: </strong>Androgen-sensitive PCa LNCaP cells were cultured with and without ADT bicalutamide, alongside ADT-resistant CRPC cells (LNCaP-ABL), either in normal or low oxygen (O₂) (1%) conditions. HIF-1α, CaV1.3, and androgen receptor (AR) gene expression was measured by qPCR and protein expression with Western blot in the presence or absence of siCaV1.3. SOCE was determined through Fura-2AM fluorescence measurement. Cell proliferation was quantified by WST-1 assay and survival by colony formation.</p><p><strong>Results: </strong>CaV1.3 expression was increased during ADT but not hypoxia, correlating with an associated increase in SOCE. HIF-1α expression was upregulated by ADT under normal O₂ conditions and increased during hypoxia across all cells but with a higher fold change in early ADT-resistant and CRPC cells. Under hypoxic conditions CaV1.3 small interfering RNA resulted in a significant reduction in HIF-1α expression for ADT-sensitive cells but increased in CRPC. A similar pattern was also observed for AR expression. Cell survival was found significantly reduced by siCaV1.3 under hypoxic conditions for all cells, with and without ADT. Whereas cell proliferation under the same conditions was reduced in CRPC only.</p><p><strong>Conclusion: </strong>This study highlights that CaV1.3 can modulated HIF signaling and impact on PCa tumor biology under hypoxia, but further investigation is required to ascertain if this mediated through SOCE or a noncanonical mechanism.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77557990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Call for <i>Special Issue Papers: Bioelectrical Signaling via Gap Junctions</i>: Deadline for Manuscript Submission: March 1, 2023.","authors":"Jose F Ek Vitorin, Jennifer S Fang","doi":"10.1089/bioe.2022.29030.cfp","DOIUrl":"10.1089/bioe.2022.29030.cfp","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79256591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}