Bioelectricity最新文献

{"title":"Electrical Stimulation in Cell Culture: Why It Is Still Not Easy or Accessible-and How We Fix It.","authors":"Ryan T Murray, Malavika Nair","doi":"10.1177/25763113261459372","DOIUrl":"10.1177/25763113261459372","url":null,"abstract":"<p><p>Growing insight into bioelectric regulation of cell behavior has driven extensive use of electrical stimulation in <i>in vitro</i> studies over the last decade, notably in the enhancement of stem cell maturation. This has prompted the development of electrical stimulation bioreactors to provide the stimulation parameters required for such studies. However, biological outcomes are highly sensitive to stimulation parameters, electrode geometry, and electrode-electrolyte interface. These are factors that current commercial and custom systems handle differently; commercial platforms are robust but often cost-prohibitive, whereas custom open-source systems are cheaper but typically lack validated, user-friendly hardware and standardization. In this perspective, we argue for openly documented electrical stimulation platforms that are not only published but also made available through open-source science manufacturers. Coupling transparent designs with distributed assembly and open validation data offers the potential to lower current barriers to entry, enabling wider adoption of pre-characterized, affordable systems that improve reproducibility and mechanistic clarity.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"161-165"},"PeriodicalIF":1.5,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13364806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bioelectricity</i> in Health Care.","authors":"Mustafa B A Djamgoz, Caterina Merla","doi":"10.1177/25763113261460787","DOIUrl":"10.1177/25763113261460787","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"109-110"},"PeriodicalIF":1.5,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MEB: \"From Development to Cognitive Glue: My Journey in Bioelectricity\".","authors":"Michael Levin","doi":"10.1177/25763113261454327","DOIUrl":"10.1177/25763113261454327","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"168-181"},"PeriodicalIF":1.5,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How One Single Eureka Moment Can Dictate a Full Scientific Career in Bioelectricity.","authors":"Lluis M Mir","doi":"10.1177/25763113261434920","DOIUrl":"10.1177/25763113261434920","url":null,"abstract":"<p><p>In science, one needs to have a eureka moment from time to time, in order to avoid falling into depression-it is not easy every day to be a researcher. Sometimes, these eurekas are the result of SERENDIPITY. However, in most of the cases, these eurekas are the result… of (experimental) RESULTS, that is, of WORK. But there is another category, the GREAT EUREKAS, that do not hit your mind many times in your life. I believe that I had only one GREAT EUREKA that, however, dictated my projects, experiments and successes for several decades.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 1","pages":"104-108"},"PeriodicalIF":1.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13165800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147934033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Sub-Microsecond Plasma Membrane Potential Shifts and Bioeffects Under Low-Energy Electric Pulse Stimulation.","authors":"Gleb P Tolstykh, Mark A Keppler, Roberto A Rodriguez, Sean P O'Connor, Joel N Bixler, Bennett L Ibey, Kevin D Richardson, Benjamin M Kasukonis","doi":"10.1177/25763113261441517","DOIUrl":"10.1177/25763113261441517","url":null,"abstract":"<p><p>Low-energy micro- and millisecond electric pulses (EPs) charge and depolarize the cellular plasma membrane (PM) below the electroporation threshold. Conversely, individual nanosecond EPs (NSEPs) are too brief to initiate PM depolarization by charging the cells. It is hypothesized that a single NSEP induces cell depolarization via PM electroporation upon application of high-power EPs. However, low-energy NSEP bursts with a very high pulse repetition frequency may prevent electroporation. This method, a temporal summation of NSEPs, results in subsequent PM charging and depolarization. To visualize PM voltage changes during low-energy EPs, we employed optical measurements using FluoVolt™ (an organic fluorescent reporter of membrane potential [MP]) and a custom-made streak imaging system. Ultra-fast streak kymographs depicting MP changes were obtained after exposure to a ∼0.2 kV/cm single 200 µs EP and 5 MHz trains of 1000 and 2000 NSEPs with 100 ns duration. Immediately following exposure, a small FluoVolt™ response (up to ∼7% fluorescence change) was observed in the PM areas facing electrodes. The response duration directly correlated with the pulse width (PW) or duration of the NSEPs burst interval. The single 200 µs EP was more effective at charging the PM than an equivalent-energy 5 MHz burst of 2000 NSEPs of 100 ns duration. Furthermore, similar amplitudes of PM fluorescence changes between ∼0.2 kV/cm bursts of 1000 and 2000 NSEPs suggest that increasing either PW or applied voltage is necessary to enhance the extent of PM depolarization. Nonetheless, the modest depolarization effect reported herein was sufficient to open voltage-gated Ca²⁺ channels in neurons. These findings indicate that a 5 MHz burst of low-energy NSEPs and single µs EPs effectively induce PM depolarization and Ca²⁺ responses without causing any cellular damage.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"139-145"},"PeriodicalIF":1.5,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Roadmap for Bioelectric Electrochemical Sensing in Cancer.","authors":"Sima Singh, Ada Raucci, Alessandra Glovi, Gabriella Iula, Stefania Galdiero, Antonio De Luca, Antonio Giordano, Michelino De Laurentiis, Stefano Cinti","doi":"10.1177/25763113261438725","DOIUrl":"10.1177/25763113261438725","url":null,"abstract":"<p><p>Real-time electrochemical measurements of ionic fluxes and bioelectric signals are set to refine cancer diagnosis and longitudinal follow-ups with the goal of targeting electrophysiological features of clinically useful matrices. Nanoengineered electrodes translate biomolecules and ions into robust electrochemical signals for both laboratory workflows and point-of-care testing. The translational aim is to integrate electrochemical sensing into coherent, physiology-grounded readouts that resolve tumor ion channel dysfunction, membrane depolarization, pericellular acidification, and redox imbalance. Realizing this vision now depends on practical engineering and clinical integration. To advance this ongoing development, researchers are building stable, disposable, portable, and miniaturized electrochemical platforms that couple detection with microfluidics to enrich tumor cells, vesicles, and bio ionic markers for multiplexed cancer measurements. The integration of wearable and implantable systems with machine learning and patient-specific digital twins will enable real-time maps of tumor electrophysiology and model-driven forecasts of treatment response. This perspective outlines a translational roadmap for electrochemical detection of bioelectric biomarkers in cancer, wherein biomarker classes are systematically mapped to corresponding transduction strategies and mechanistic fidelity is reconciled with practical assay design constraints to identify key challenges and opportunities for advancing these technologies from foundational research to validated clinical implementation in precision oncology.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"154-160"},"PeriodicalIF":1.5,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane Voltage Profiling: A Bioelectricity-Based Intraoperative Imaging Methodology for Rapid Surgical Margin Analysis.","authors":"Dany Spencer Adams, Dina Seremet, Omar Al-Kofahi","doi":"10.1177/25763113261433466","DOIUrl":"10.1177/25763113261433466","url":null,"abstract":"<p><p>Surgeons face serious challenges removing cancer fully during surgery, with incomplete cancer removal posing significant risks to patients. To address this problem, Lucell Diagnostics Inc. is developing an innovative cancer detection platform called membrane voltage profiling (MVPro; patent pending). This groundbreaking method exploits the discovery that cancer cells exhibit a physiological biomarker, depolarization, as revealed by fluorescent voltage-sensitive dyes. Cancer cells fluoresce in specific patterns and intensities that differ from normal cells, allowing precise identification. We present here our preliminary results on the feasibility of using voltage to locate cancer cells. The aims were: to perform controls showing whether MVPro affects the normal pathology process; to optimize tissue transfer for margin cell collection; to confirm that the VSD DiBAC₄(3) is appropriate for MVPro of skin cells; to determine whether MVPro finds cancer in the same specimens as pathological analysis. These studies on nonmelanoma skin cancer specimens reveal that MVPro is low risk to the patient, integrates with existing surgical protocols, finds cancer in the same specimens as does pathology, and presents no complications for pathological analysis. Once development of this methodology is complete, MVPro will yield an annotated, 2D heat map covering the entire surgical margin, indicating the location of cells with a high likelihood of being cancer. This will empower surgeons to confirm a negative surgical margin before closing. This simple type of intraoperative imaging, performed in or near the operating theater, has the potential to improve surgical outcome, cut health care costs, and enhance post-surgical quality of life by preserving healthy tissue. Because the setup costs are relatively small and the reagents are inexpensive, we believe MVPro could be of great benefit to underserved areas. Indeed, MVPro could benefit health care systems globally, from cutting-edge hospitals to small clinics in underserved regions.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"146-153"},"PeriodicalIF":1.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectricity Buzz.","authors":"Ann M Rajnicek","doi":"10.1177/25763113261433469","DOIUrl":"10.1177/25763113261433469","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 1","pages":"97-103"},"PeriodicalIF":1.1,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13165811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147933892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of Contactless High Intensity Pulsed Electromagnetic Field Induced Electroporation and Gene Delivery Efficacy Using Various Nanoparticles.","authors":"Tamara Polajžer, Matej Kranjc, Slavko Kralj, Maja Caf, Rok Romih, Samo Hudoklin, Vitalij Novickij, Damijan Miklavčič","doi":"10.1177/25763113261423900","DOIUrl":"10.1177/25763113261423900","url":null,"abstract":"<p><strong>Introduction: </strong>High-intensity pulsed electromagnetic fields (HI-PEMF) can be used to trigger contactless permeabilization of the plasma membrane similar to electroporation (EP). The permeabilization efficiency and gene delivery by HI-PEMF <i>in vitro</i> are currently inferior to EP. It was suggested that the methodology can be improved with conductive gold nanoparticles (AuNPs), which are reported to amplify the induced electric field in close proximity to the cell membrane.</p><p><strong>Objectives: </strong>Therefore, in this work, we have studied different NPs, which varied in material/conductivity (gold and silica), size (10-50+ nm), shape (i.e., round and rods), concentration (50-200 µg/mL), and functionalization (pegylated or not), and combined them with HI-PEMF (6.7 T × 100 pulses, 1 Hz).</p><p><strong>Methods: </strong>The normal Chinese hamster ovary cell line (CHO) and the cancer human urinary bladder's transitional carcinoma cell line (T24) were used as a model. We have characterized cell membrane permeabilization using propidium iodide (PI) and the efficacy of gene delivery using pEGFP-N1.</p><p><strong>Results: </strong>Larger NPs and higher NP concentrations resulted in up to a 10% increase in membrane permeability. In contrast, semispherical and rod-shaped AuNPs did not further enhance permeabilization efficiency. Gene delivery efficiency increased from 3% in control samples to 6% in the presence of 50 nm AuNPs. Overall, CHO cells were more susceptible to HI-PEMF-induced effects than T24 cells.</p><p><strong>Conclusions: </strong>This study shows the potential to increase gene delivery efficacy by combining HI-PEMF treatment with conductive NPs. However, it was concluded that the HI-PEMF-induced effects are highly dependent on the cell line, NP type, and concentration and therefore require further investigation.</p>","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 1","pages":"67-77"},"PeriodicalIF":1.1,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13165809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147933996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibiting Metastasis Through Ion Channels: Combinatorial Treatment in accordance with the CELEX Model?","authors":"Mustafa B A Djamgoz","doi":"10.1177/25763113261418673","DOIUrl":"10.1177/25763113261418673","url":null,"abstract":"","PeriodicalId":29923,"journal":{"name":"Bioelectricity","volume":"8 2","pages":"166-167"},"PeriodicalIF":1.5,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}