Dental Materials最新文献

{"title":"The effects of monomer type, filler size, and filler content of three resin cements on the color stability of laminate veneers exposed to accelerated aging","authors":"","doi":"10.1016/j.dental.2024.06.001","DOIUrl":"10.1016/j.dental.2024.06.001","url":null,"abstract":"<div><h3>Statement of problem</h3><p>Commercially available resin cements consist of various filler sizes, filler content and monomers, and it is unclear which of these factors have the greatest effect on the color stability of laminate veneers.</p></div><div><h3>Objectives</h3><p>To evaluate the color stability of lithium disilicate-reinforced laminate veneers inserted with three commercial resin cements with various filler sizes/contents and monomers upon accelerated aging.</p></div><div><h3>Methods</h3><p>Veneers were fabricated and cemented on resin abutments using three commercially available resin cements comprised of two different monomers and varying filler sizes/contents: 1) triethylene glycol dimethacrylate, TEGDMA (RelyX™ Veneer = 0.6 µm/66 %, Calibra® Esthetic Light Cure =1.2 µm/65 %); and 2) urethane dimethacrylate, UDMA (Variolink Esthetic LC = 0.1 µm/38 %). A total of 60 specimens were fabricated (n = 20 for each cement). The relative number of particles (N) and relative surface area (A) were calculated for each filler size/content. Color coordinates (L*, a*, b*) were measured, and color change (ΔE₀₀) was calculated after cementation and following aging at 150, 300, 450, and 600 h using xenon light. Differences in color coordinates and color change were determined using repeated measures ANOVA followed by Tukey's post hoc test (α = .05). A post-hoc power analysis was performed to confirm reliability of the results.</p></div><div><h3>Results</h3><p>Based on a post-hoc power analysis of a repeated measures ANOVA with two between-factors and 1 within-factor, we had 89 % power to detect a difference of effect by cement type, 10 % power to detect a difference by shade, and 100 % power to detect a difference of effect by aging. The UDMA-based cement (0.1 µm/38 %) was least affected by aging, despite having the largest number of particles (N = 1010) and largest particle surface area (A = 7.02). The TEGDMA-based cements exhibited a significant color change, with 0.6 µm/66 % (A = 2.03, N = 8.12) producing a larger ΔE₀₀ than 1.2 µm/65 % (A = 1.00, N = 1.00).</p></div><div><h3>Conclusions</h3><p>Among the commercial cements tested, UDMA-based resin (0.1 µm/38 %) provided better color stability than TEGDMA-based resin cements. The color change after aging was affected by the relative surface area and relative number of particles for the TEGDMA-based resin cement (0.6 µm/66 % and 1.2 µm/65 %), with a larger surface area and a higher relative number of particles, accelerated color change with aging. The present study demonstrates a novel approach to determine color stability for any resin cement with particulate filler.</p></div><div><h3>Clinical implications</h3><p>The dental practitioner should be selective in choosing a commercial light cure cementation product for laminate veneers, as UDMA containing resin cement is more resistant to color changes over time than TEGDMA, regardless of the filler size/content.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 8","pages":"Pages e23-e30"},"PeriodicalIF":4.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Permeability of triamcinolone acetonide, released from mucoadhesive films, through a buccal mucosa-mimetic barrier: Permeapad™","authors":"","doi":"10.1016/j.dental.2024.06.016","DOIUrl":"10.1016/j.dental.2024.06.016","url":null,"abstract":"<div><h3>Objectives</h3><p>The permeability of triamcinolone acetonide (TA), from bilayer mucoadhesive buccal films, through a biomimetic membrane, Permeapad™, was investigated employing Franz diffusion cell. The delivery systems composition and ethyl cellulose (EC) backing layer, on drug permeability, were assessed.</p></div><div><h3>Methods</h3><p>Three TA-loaded films were tested; hydroxypropyl methylcellulose (HPMC K4M; bilayer [F1] and monolayer), HPMC K4M/Polyvinylpyrrolidone (PVP): 90/10 [F2], and HPMC K15M film [F3]. All films contained propylene glycol (PG-plasticiser). TA solution alone was used as a control. TA permeability via a Permeapad™ barrier, simulating buccal mucosa, was assessed over 8 h using a Franz diffusion cell. TA permeated into the receptor compartment, released in the donor compartment, and located on/within the Permeapad™ barrier were analysed using UV-spectrophotometer.</p></div><div><h3>Results</h3><p>45.7 % drug retention within the Permeapad™ barrier was delivered from F1 (highest). F1, F2, and F3 significantly improved the TA’s permeability through Permeapad™, compared to TA solution alone (e.g., 8.5 % TA-solution, 21.5 %-F1), attributed to the synergy effect of HPMC and propylene glycol acting as penetration enhancers. F1 displayed a significant increase in drug permeability (receptor compartment; 21.5 %) compared to F3 (17.0 %). PVP significantly enhanced drug permeability (27.5 %). Impermeable EC backing layer controlled unidirectional drug release and reduced drug loss into the donor compartment (e.g., ∼28 % for monolayer film to ∼10 % for bilayer film, F1).</p></div><div><h3>Significance</h3><p>The mucoadhesive films demonstrated improved TA permeability via Permeapad™. The findings suggest that these bilayer mucoadhesive films, particularly F1, hold promise for the effective topical treatment of oral mucosa disorders, such as recurrent aphthous stomatitis and oral lichen planus.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1372-1377"},"PeriodicalIF":4.6,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124001581/pdfft?md5=a82b18d9a685e969cc76ee3d2bd1d93d&pid=1-s2.0-S0109564124001581-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of baghdadite (Ca3ZrSi2O9) cements for the application as novel endodontic filling materials","authors":"","doi":"10.1016/j.dental.2024.05.026","DOIUrl":"10.1016/j.dental.2024.05.026","url":null,"abstract":"<div><h3>Objectives</h3><p>Baghdadite (Ca₃ZrSi₂O₉) cements of various composition have been investigated in this study regarding an application as endodontic filling materials.</p></div><div><h3>Methods</h3><p>Cements were either obtained by mixing mechanically activated baghdadite powder with water (maBag) or by subsequently substituting the ß-tricalcium phosphate (ß-TCP) component in a brushite forming calcium phosphate cement. The cements were analyzed for their mechanical performance, injectability, radiopacity, phase composition and antimicrobial properties.</p></div><div><h3>Results</h3><p>The cements demonstrated sufficient mechanical performance with a compressive strength of ∼1 MPa (maBag) and 2.3 – 17.4 MPa (substituted calcium phosphate cement), good injectability &gt; 80 % depending on the powder to liquid ratio and an intrinsic radiopacity of 1.13 – 2.05 mm aluminum equivalent. Immersion in artificial saliva proved their bioactivity by the formation of calcium phosphate and calcium silicate precipitates on the cement surface. The bacterial activity of <em>Staphylococcus aureus</em> cultured on the surface of the cements was found to be similar compared to clinical standard ProRoot MTA cement or even reduced by a factor of 3 for <em>Streptococcus mutans</em>.</p></div><div><h3>Significance</h3><p>In combination with their antibacterial properties, baghdadite cements are thought to have the potential to fulfil the clinical requirements for endodontic filling materials.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1364-1371"},"PeriodicalIF":4.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124001246/pdfft?md5=ed16838169369752ef00adc0baa750d4&pid=1-s2.0-S0109564124001246-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Cention-N comparable to other direct dental restorative materials? A systematic review with network meta-analysis of in vitro studies","authors":"","doi":"10.1016/j.dental.2024.06.014","DOIUrl":"10.1016/j.dental.2024.06.014","url":null,"abstract":"<div><h3>Objectives</h3><p>To compare the performance of Cention-N® with direct restorative materials used at the daily practice (e.g., resin-based composites/RBC, glass ionomer cements/GIC, bioactive resins, silver amalgam) via a systematic review study.</p></div><div><h3>Methods</h3><p>The review followed the PRISMA-NMA recommendations, and the protocol of the review was published at osf.io/ybde8. The search was conducted in PubMed/MEDLINE, Scopus, Web of Science, Embase, Lilacs, and SciELO databases, as well as in the grey literature (Open Grey, Proquest, and Periódicos CAPES). Studies with an in vitro experimental design evaluating the characteristics and properties of Cention-N in comparison to other restorative materials were included. The risk of bias of included studies was assessed using the RoBDEMAT tool, and meta-analyses were conducted using Review Manager 5.4 and MetaInsight V3 tools.</p></div><div><h3>Results</h3><p><span>A total of 85 studies were included in the review, from which 79 were meta-analyzed. Several characteristics of direct restorative materials were analyzed, including physical (color change, degree of conversion, hardness, microleakage, polymerization rate, roughness, water solubility, water sorption), mechanical (bond strength to dentin, compressive strength, diametral tensile strength, flexural modulus, flexural strength, load-to-fracture, wear), and biological (alkalinizing effect, </span>antibacterial activity, calcium and fluoride release) properties.</p></div><div><h3>Significance</h3><p>Cention-N presented similar physico-mechanical properties compared to RBCs, but a stronger behavior than GICs. Despite the Alkasite nature of Cention-N, GICs may still demonstrate the greatest fluoride releasing ability from all direct restorative materials. This review confirmed the adequate behavior of Cention-N when compared to several other more traditionally used materials, confirming its applicability for the permanent restoration of decayed or fractured teeth.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1341-1352"},"PeriodicalIF":4.6,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141329894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longevity of different abutments placed on narrow diameter implants: Assessment of structural damage and loosening","authors":"","doi":"10.1016/j.dental.2024.06.003","DOIUrl":"10.1016/j.dental.2024.06.003","url":null,"abstract":"<div><h3>Objectives</h3><p>To evaluate structural damage and loosening of abutments placed on narrow diameter implants after cyclic fatigue.</p></div><div><h3>Methods</h3><p><span>Sixty Morse taper narrow diameter implants (Neodent, Brazil) received two types of abutments (1PA- one-piece abutment or 2PA- two-piece abutment), which were randomly divided into 3 fatigue experiments (n = 10). The implants were placed into a customized supporting holder and a software-assisted digital torque wrench secured the manufacturer recommended torque for each abutment. Cone beam computed tomography (CBCT) scans were acquired, before and after fatigue, and post-processed (software e-Vol DX) to assess damage and abutment displacement. The boundary fatigue method was adapted to use 2 × 10</span>⁶ cycles, 2 Hz of frequency and constant peak load of 80 N (first experiment) that varied according to the failure rate of previous specimens (second and third experiments). Failure was evaluated using CBCT scans and removal torque values. Data were used to estimate long-term torque degradation, probability of failure and Weibull modulus (software ALTA PRO9).</p></div><div><h3>Results</h3><p>All 2PA specimens became loosen independently of the applied fatigue load, and structural bending was observed in 14 abutments. Eight 1PA got loosen during the fatigue experiment. The Weibull analysis showed a lower modulus (<em>m</em> = 1.0; 0.7, 1.4) for 1PA than for 2PA (<em>m</em> = 2.6; 2, 3.4) resulting in longer predicted lifetimes and slower torque degradation for 1PA than for 2PA specimens.</p></div><div><h3>Significance</h3><p>1PA showed greater long-term survival probability than 2PA. Predicting the lifetime and mechanical behavior of implant-abutment systems are useful information to clinicians during the decision-making process of oral rehabilitations.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1332-1340"},"PeriodicalIF":4.6,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifunctional naringenin-laden gelatin methacryloyl scaffolds with osteogenic and anti-inflammatory properties","authors":"","doi":"10.1016/j.dental.2024.06.019","DOIUrl":"10.1016/j.dental.2024.06.019","url":null,"abstract":"<div><h3>Objective</h3><p>To fabricate and characterize an innovative gelatin methacryloyl/GelMA electrospun scaffold containing the citrus flavonoid naringenin/NA with osteogenic and anti-inflammatory properties.</p></div><div><h3>Methods</h3><p><span>GelMA scaffolds (15 % w/v) containing 0/Control, 5, 10, or 20 % of NA w/w were obtained via electrospinning. The chemical composition, fiber morphology/diameter, swelling/degradation profile, and NA release were investigated. Cytotoxicity, cell proliferation, adhesion and spreading, total protein/TP production, alkaline phosphatase/ALP activity, osteogenic genes expression (OCN, </span>OPN<span>, RUNX2), and mineralized nodules deposition/MND with human alveolar bone-derived mesenchymal stem cells (aBMSCs) seeded on the scaffolds were assessed. Moreover, aBMSCs seeded on the scaffolds and stimulated with tumor necrosis factor-alpha/TNF-α were submitted to collagen, nitric oxide/NO, interleukin/IL-1α, and IL-6 production assessment. Data were analyzed using ANOVA and t-student/post-hoc tests (α = 5 %).</span></p></div><div><h3>Results</h3><p><span>NA-laden scaffolds presented increased fiber diameter, lower swelling capacity, and faster degradation profile over 28 days (p &lt; 0.05). NA release was detected over time. Cell adhesion and spreading, and TP production were similar between GelMA and GelMA+NA5 % scaffolds, while cell proliferation, ALP activity, OCN/OPN/RUNX2 gene expression, and MND were higher for GelMA+NA5 % scaffolds (p &lt; 0.05). Cells seeded on control scaffolds and TNF-α-stimulated presented higher levels of NO, IL-1α/IL-6, and lower levels of collagen (p &lt; 0.05). In contrast, cells seeded on GelMA+NA5 % scaffolds showed </span>downregulation<span> of inflammatory markers and higher collagen synthesis (p &lt; 0.05).</span></p></div><div><h3>Significance</h3><p>GelMA+NA5 % scaffold was cytocompatible, stimulated aBMSCs proliferation and differentiation, and downregulated inflammatory mediators’ synthesis, suggesting its therapeutic effect as a multi-target bifunctional scaffold with osteogenic and anti-inflammatory properties for bone tissue engineering.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1353-1363"},"PeriodicalIF":4.6,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 10-MDP calcium salt on osteoblasts and fibroblasts","authors":"","doi":"10.1016/j.dental.2024.06.015","DOIUrl":"10.1016/j.dental.2024.06.015","url":null,"abstract":"<div><h3>Objective</h3><p><span>10-methacryloyloxidecyl dihydrogen phosphate monomer<span> (10-MDP) is commonly used as a bonding monomer<span> in universal adhesives. Adhesives that contain this monomer can directly contact the surrounding periodontium due to the chemical binding of 10-MDP with hydroxyapatite in hard tissue to form </span></span></span>calcium salts<span>. However, the effect of these calcium salts<span> on the periodontium in the case of subgingival fillings remains poorly understood. The objective of this study was to investigate effects of 10-MDP calcium salts on osteoblasts and fibroblasts in the periodontal tissues.</span></span></p></div><div><h3>Methods</h3><p>This study investigated the effects of different concentrations of 10-MDP calcium salts on the migration, proliferation, and differentiation of osteoblasts (MC3T3-E1) and fibroblasts (L929); additionally, the effect on apoptosis<span><span><span> and matrix metalloproteinases (MMPs) expression in these cells was evaluated. </span>Cell proliferation assay, </span>alkaline phosphatase<span> (ALP) activity assay, Western blotting, and quantitative real-time polymerase chain reaction were performed to determine the effects.</span></span></p></div><div><h3>Results</h3><p><span>The 10-MDP calcium salts (within a concentration of 0.5 mg/mL) showed no cytotoxicity and did not seem to influence the apoptosis, mitochondrial membrane potential<span><span>, and reactive oxygen species (ROS) levels in the cells. However, they had an inhibitory effect on the secretion of </span>MMP2<span> and MMP9 in the osteoblasts and fibroblasts. The ALP activity assay and </span></span></span>Alizarin Red staining did not reveal any significant effects of the 10-MDP calcium salts on osteoblast differentiation.</p></div><div><h3>Significance</h3><p>These results suggest that applying 10-MDP-containing adhesives to subgingival fillings may be safe and beneficial for the periodontal tissues.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1322-1331"},"PeriodicalIF":4.6,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of an ultra-bright real-time high-throughput renilla luciferase assay for antibacterial assessment of Streptococcus mutans biofilms","authors":"","doi":"10.1016/j.dental.2024.06.012","DOIUrl":"10.1016/j.dental.2024.06.012","url":null,"abstract":"<div><h3>Objective</h3><p><span>The present work demonstrates the optimization of a renilla-based real-time, ultra-bright, non-disruptive, high-throughput bioluminescence assay (HTS) to assess the metabolism of intact </span><span><em>Streptococcus</em><em> mutans</em></span><span><span> biofilms and its utility in screening the antibacterial efficacy of experimental nanofilled dental adhesive resins containing varying concentrations of nitrogen-doped titanium dioxide </span>nanoparticles (N_TiO</span>₂<span>). Methods. Optimization of the assay was achieved by screening real-time bioluminescence changes in intact </span><span><span>Streptococcus mutans</span></span><span> biofilms imposed by the various experimental biofilm growth parameters investigated (bacterial strain, growth media, sucrose concentration, dilution<span><span> factor, and inoculum volume). The optimized assay was then used to characterize the antibacterial efficacy of experimental nanofilled dental adhesive resins. The assay’s ability to discriminate between bacteriostatic and bactericidal approaches was also investigated. Results. Relative Light Units (RLU) values from the HTS optimization were analyzed by multivariate ANOVA<span> (α = 0.05) and coefficients of variation. An optimized HTS bioluminescence assay was developed displaying RLUs values (brightness) that are much more intense when comparing to other previously reported bioluminescence assays, thereby decreasing the error associated with bioluminescence assays and displaying better utility while investigating the functionalities of antimicrobial nanofilled experimental dental adhesive resins with proven long-term properties. Significance. The present study is anticipated to positively impact subsequent research on dental materials and </span></span>oral microbiology because it serves as a valuable screening tool in metabolic-based assays with increased sensitivity and robustness. The assay reported is anticipated to be further optimized to be used as a co-reporter for other Luc based assays.</span></span></p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 9","pages":"Pages 1313-1321"},"PeriodicalIF":4.6,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transdentinal effects of S-PRG fillers on odontoblast-like cells","authors":"","doi":"10.1016/j.dental.2024.06.002","DOIUrl":"10.1016/j.dental.2024.06.002","url":null,"abstract":"<div><h3>Objectives</h3><p>To investigate the transdentinal effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) fillers on odontoblast-like cells.</p></div><div><h3>Methods</h3><p>An eluate of S-PRG fillers was obtained by dissolving the particles in distilled water (1:1 m/v). Dentin discs with similar permeability were mounted into artificial pulp chambers and MDPC-23 cells were seeded on their pulpal surface. The occlusal surface was treated with (n = 10): ultrapure water (negative control – NC), hydrogen peroxide (positive control – PC), S-PRG eluate exposure for 1 min (S-PRG 1 min), or S-PRG filler eluate exposure for 30 min (S-PRG 30 min). After 24 h, cell viability (alamarBlue) and morphology (SEM) were evaluated. The extract obtained from transdentinal diffusion was applied to MDPC-23 pre-cultured in plates for another 24 h to evaluate viability (alamarBlue, 1, 3, and 7 days), gene expression of <em>Col1a1</em>, <em>Alpl</em>, <em>Dspp</em>, and <em>Dmp1</em> (RT-qPCR, 1 and 7 days), and mineralization (Alizarin Red, 7 days). Data were analyzed with ANOVA (α = 5 %).</p></div><div><h3>Results</h3><p>While S-PRG 1 min did not differ from NC, S-PRG 30 min reduced 17.9 % viability of cells from discs. S-PRG treatments resulted in low cell detaching from dentin, and the remaining cells exhibited typical morphology or minor cytoplasmic contraction. S-PRG 30 min slightly increased cell viability (6 %) 1 day after contact with the extract. S-PRG treatments upregulated the expression of the investigated genes, especially after 1 day. S-PRG 30 min stimulated mineralization activity by 39.7 %.</p></div><div><h3>Conclusions</h3><p>S-PRG filler eluate does not cause transdentinal cytotoxicity on odontoblast-like cells, and long-term exposure can stimulate their dentinogenic-related mineralization activity.</p></div><div><h3>Significance</h3><p>The transdentinal elution of ions from S-PRG fillers is not expected to be harmful to the dental pulp and may exert bioactive effects by inducing dentin matrix deposition through the metabolism of underlying odontoblasts.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 8","pages":"Pages 1259-1266"},"PeriodicalIF":4.6,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing dentin remineralization: Exploring amorphous calcium phosphate and its stabilizers in biomimetic approaches","authors":"","doi":"10.1016/j.dental.2024.06.013","DOIUrl":"10.1016/j.dental.2024.06.013","url":null,"abstract":"<div><h3>Objective</h3><p>This review elucidates the mechanisms underpinning intrafibrillar mineralization, examines various amorphous calcium phosphate (ACP) stabilizers employed in dentin’s intrafibrillar mineralization, and addresses the challenges encountered in clinical applications of ACP-based bioactive materials.</p></div><div><h3>Methods</h3><p>The literature search for this review was conducted using three electronic databases: PubMed, Web of Science, and Google Scholar, with specific keywords. Articles were selected based on inclusion and exclusion criteria, allowing for a detailed examination and summary of current research on dentin remineralization facilitated by ACP under the influence of various types of stabilizers.</p></div><div><h3>Results</h3><p>This review underscores the latest advancements in the role of ACP in promoting dentin remineralization, particularly intrafibrillar mineralization, under the regulation of various stabilizers. These stabilizers predominantly comprise non-collagenous proteins, their analogs, and polymers. Despite the diversity of stabilizers, the mechanisms they employ to enhance intrafibrillar remineralization are found to be interrelated, indicating multiple driving forces behind this process. However, challenges remain in effectively designing clinically viable products using stabilized ACP and maximizing intrafibrillar mineralization with limited materials in practical applications.</p></div><div><h3>Significance</h3><p>The role of ACP in remineralization has gained significant attention in dental research, with substantial progress made in the study of dentin biomimetic mineralization. Given ACP’s instability without additives, the presence of ACP stabilizers is crucial for achieving <em>in vitro</em> intrafibrillar mineralization. However, there is a lack of comprehensive and exhaustive reviews on ACP bioactive materials under the regulation of stabilizers. A detailed summary of these stabilizers is also instrumental in better understanding the complex process of intrafibrillar mineralization. Compared to traditional remineralization methods, bioactive materials capable of regulating ACP stability and controlling release demonstrate immense potential in enhancing clinical treatment standards.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 8","pages":"Pages 1282-1295"},"PeriodicalIF":4.6,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}