Dental Materials最新文献

{"title":"Guidance on the assessment of biocompatibility of biomaterials: Fundamentals and testing considerations","authors":"Vinicius Rosa ,&nbsp;Nikolaos Silikas ,&nbsp;Baiqing Yu ,&nbsp;Nileshkumar Dubey ,&nbsp;Gopu Sriram ,&nbsp;Spiros Zinelis ,&nbsp;Adriano F. Lima ,&nbsp;Marco C. Bottino ,&nbsp;Joao N. Ferreira ,&nbsp;Gottfried Schmalz ,&nbsp;David C. Watts","doi":"10.1016/j.dental.2024.07.020","DOIUrl":"10.1016/j.dental.2024.07.020","url":null,"abstract":"<div><h3>Background</h3><div>Assessing the biocompatibility of materials is crucial for ensuring the safety and well-being of patients by preventing undesirable, toxic, immune, or allergic reactions, and ensuring that materials remain functional over time without triggering adverse reactions. To ensure a comprehensive assessment, planning tests that carefully consider the intended application and potential exposure scenarios for selecting relevant assays, cell types, and testing parameters is essential. Moreover, characterizing the composition and properties of biomaterials allows for a more accurate understanding of test outcomes and the identification of factors contributing to cytotoxicity. Precise reporting of methodology and results facilitates research reproducibility and understanding of the findings by the scientific community, regulatory agencies, healthcare providers, and the general public.</div></div><div><h3>Aims</h3><div>This article aims to provide an overview of the key concepts associated with evaluating the biocompatibility of biomaterials while also offering practical guidance on cellular principles, testing methodologies, and biological assays that can support in the planning, execution, and reporting of biocompatibility testing.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 11","pages":"Pages 1773-1785"},"PeriodicalIF":4.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated preventive and therapeutic magnetic nanoparticle loaded with rhamnolipid and vancomycin for combating subgingival biofilms","authors":"Wenyan Kang ,&nbsp;Ting Zou ,&nbsp;Ye Liang ,&nbsp;Huaxiang Lei ,&nbsp;Rui Zhang ,&nbsp;Jun Kang ,&nbsp;Zhenquan Sun ,&nbsp;Xuechen Li ,&nbsp;Shaohua Ge ,&nbsp;Chengfei Zhang","doi":"10.1016/j.dental.2024.08.005","DOIUrl":"10.1016/j.dental.2024.08.005","url":null,"abstract":"<div><h3>Objectives</h3><div>Mechanical debridement supplemented with antibacterial agents effectively eradicates subgingival biofilms formed in the periodontal pockets of severe periodontitis patients. However, the available antimicrobial agents have limited penetrating ability to kill the bacteria encased in the deep layers of biofilms. This study aimed to fabricate a novel magnetic nanoparticle (MNP) loaded with rhamnolipid (RL) and vancomycin (Vanc, Vanc/RL-Ag@Fe₃O₄) to combat subgingival biofilms.</div></div><div><h3>Methods</h3><div>The multispecies subgingival biofilm was formed by periodontal pathogens, including <em>Streptococcus oralis</em> (<em>S. oralis</em>), <em>Streptococcus sanguinis</em> (<em>S. sanguinis</em>), <em>Actinomyces naeslundii</em> (<em>A. naeslundii</em>), <em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>) and <em>Fusobacterium nucleatum</em> (<em>F. nucleatum</em>). Scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and quantitative real-time polymerase chain reaction (qRT-PCR) were used to determine the anti-biofilm efficacy of Vanc/RL-Ag@Fe₃O₄ with or without a magnetic field on multispecies subgingival biofilms.</div></div><div><h3>Results</h3><div>The minimal inhibitory concentration (MIC) values of Vanc/RL-Ag@Fe₃O₄ on <em>S. oralis</em>, <em>S. sanguinis</em>, <em>A. naeslundii</em>, <em>P. gingivalis</em>, and <em>F. nucleatum</em> were 25, 50, 100, 50, and 25 μg/mL, respectively. Vanc/RL-Ag@Fe₃O₄ (200 μg/mL) reduced the 7-d biofilm thickness from 22 to 13 µm by degrading extracellular polymeric substance (EPS) and killing most bacteria except for tolerant <em>F. nucleatum</em>. A magnetic field enhanced the anti-biofilm effect of Vanc/RL-Ag@Fe₃O₄ by facilitating its penetration into the bottom layers of biofilms and killing tolerant <em>F. nucleatum</em>.</div></div><div><h3>Significance</h3><div>Vanc/RL-Ag@Fe₃O₄ MNPs can release RL, Vanc, and Ag and eradicate subgingival biofilms by disrupting EPS and killing bacteria. Vanc/RL-Ag@Fe₃O₄ combined with a magnetic force is a promising approach for combating periodontal infection.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 11","pages":"Pages 1808-1822"},"PeriodicalIF":4.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sol-gel-derived calcium silicate cement incorporating collagen and mesoporous bioglass nanoparticles for dental pulp therapy","authors":"Hazel O. Simila ,&nbsp;Caroline Anselmi ,&nbsp;Lais M. Cardoso ,&nbsp;Renan Dal-Fabbro ,&nbsp;Ana M. Beltrán ,&nbsp;Marco C. Bottino ,&nbsp;Aldo R. Boccaccini","doi":"10.1016/j.dental.2024.08.006","DOIUrl":"10.1016/j.dental.2024.08.006","url":null,"abstract":"<div><h3>Objective</h3><div>Calcium silicate cements (CSCs) are often used in endodontics despite some limitations related to their physical properties and antibacterial efficacy. This study aimed to develop and demonstrate the viability of a series of CSCs that were produced by sol-gel method and further modified with mesoporous bioactive glass nanoparticles (MBGNs) and collagen, for endodontic therapy.</div></div><div><h3>Methods</h3><div>Calcium silicate (CS) particles and MBGNs were synthesized by the sol-gel method, and their elemental, molecular, and physical microstructure was characterized. Three CSCs were developed by mixing the CS with distilled water (CS+H₂O), 10 mg/mL collagen solution (CS+colH₂O), and MBGNs (10 %) (CSmbgn+colH₂O). The mixing (MT) and setting (ST) times of the CSCs were determined, while the setting reaction was monitored in real-time. Antibacterial efficacy against <em>Enterococcus faecalis</em> (<em>E. faecalis</em>) and regenerative potential on dental pulp stem cells (DPSCs) were also analyzed.</div></div><div><h3>Results</h3><div>The CS+H₂O displayed a ST comparable to commercial products, while CSmbgn+colH₂O achieved the longest MT of 68 s and the shortest ST of 8 min. All the experimental CSCs inhibited the growth of <em>E. faecalis</em>. Additionally, compared to the control group, CSCs supported cell proliferation and spreading and mineralized matrix production, regardless of their composition.</div></div><div><h3>Significance</h3><div>Tested CSCs presented potential as candidates for pulp therapy procedures. Future research should investigate the pulp regeneration mechanisms alongside rigorous antibacterial evaluations, preferably with multi-organism biofilms, executed over extended periods.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 11","pages":"Pages 1832-1842"},"PeriodicalIF":4.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate analysis of titanium and PolyEtherEtherKetone materials as an alternative to cobalt-chrome framework in removable partial denture: A systematic review","authors":"Karine Bertotti ,&nbsp;Julia Mwenge-Wambel ,&nbsp;Christophe Sireix ,&nbsp;Olivier Hüe ,&nbsp;Christophe Jeannin ,&nbsp;Brigitte Grosgogeat","doi":"10.1016/j.dental.2024.07.036","DOIUrl":"10.1016/j.dental.2024.07.036","url":null,"abstract":"<div><h3>Statement of problem</h3><div>New materials have emerged in the dental field to replace the cobalt-chrome (CoCr) alloy used for the metal frameworks in removable partial denture (RPD) such as Titanium (Ti) and PolyEtherEtherKetone (PEEK). However, few studies have demonstrated their mechanical and biological performance.</div></div><div><h3>Purpose</h3><div>The purpose of this systematic review was to compare the performance of Ti and PEEK in RPD using CoCr metal framework as a reference.</div></div><div><h3>Material and methods</h3><div>This review follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Three data bases were analyzed, including PubMed/MEDLINE, Embase and Web of Science before March 2024. Only studies assessing the mechanical and/or biological properties of RPD in Ti, PEEK and CoCr were included. The quality of the studies was assessed by using the software Rayyan. The risks of bias were assessed with the methodological index for nonrandomized studies (MINORS). The mechanical (retention force, fatigue life, deformation strength, machinability, rigidity, porosity and surface roughness) and biological (plaque indices, ion release and biocompatibility) aspects were assessed.</div></div><div><h3>Results</h3><div>Among 138 articles identified, only 18 studies were included in this review. Majority had a low to moderate risk of bias. Retention forces and fatigue were significantly lower for Ti and PEEK than for CoCr, and the same was true for Ti rigidity. PEEK showed less deformation.</div><div>Both materials were suitable for machining. In terms of biological properties, both materials showed adequate biocompatibility for clinical use.</div></div><div><h3>Conclusion</h3><div>Ti and PEEK seems to be promising as alternative materials to CoCr frameworks for RPD, in terms of both their mechanical and biological performance. However, additional studies are needed to better understand their clinical and long-term limitations to enable the best-informed clinical choice for the patients and the professionals.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 11","pages":"Pages 1854-1861"},"PeriodicalIF":4.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and osteogenic assessment of experimental fluoride-doped calcium-phosphate cements on human dental pulp stem cells","authors":"Carmela Del Giudice ,&nbsp;Gianrico Spagnuolo ,&nbsp;Ciro Menale ,&nbsp;Yu Fu Chou ,&nbsp;Juan Manuel Núñez Martí ,&nbsp;Carlo Rengo ,&nbsp;Sandro Rengo ,&nbsp;Salvatore Sauro","doi":"10.1016/j.dental.2024.09.019","DOIUrl":"10.1016/j.dental.2024.09.019","url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigated the impact of some specific experimental calcium phosphate cements doped with different fluoride salts (FDCPCs) concentrations on the basal functions of human Dental Pulp Stem Cells (hDPSCs). Furthermore, this study also examined the migration, as well as the mineralisation through osteogenic differentiation.</div></div><div><h3>Methods</h3><div>Experimental FDCPCs were formulated using different concentrations of calcium/sodium fluoride salts [(5 wt%: VS5F), (10 wt%: VS10F), (20 wt%: VS20F)]. A fluoride-free calcium phosphate (VS0F) was used as a control. The hDPSCs were assessed to evaluate their self-renewal and migration activity in the presence of eluates of the different FDCPCs. A viability assay in osteogenic conditions was carried out, along with the differentiation potential through Alkaline Phosphatase Activity (ALP), and Alizarin Red Staining (ARS). Moreover, the gene expression of specific markers (RUNX2, ALP, COL1α1, OCN, OPN, DSPP, MEPE, and DMP-1) was also evaluated.</div></div><div><h3>Results</h3><div>All the tested FDCPD had no influence on cell migrations, but they caused a decrease in cell viability in osteogenic conditions when not diluted. Conversely, the eluants of VS20F showed a positive effect on stem cell differentiation. This result was corroborated through ALP activity, ARS assay. Moreover, upregulation of specific gene markers such as RUNX2, DMP-1, and DSPP was observed in hDPSCs, especially when treated with VS20F.</div></div><div><h3>Significance</h3><div>The experimental FDCPC tested in this study exhibits a dose-dependent capacity to promote mineralisation in osteogenic environment. The FDCPC-VS20F seems to be the most promising experimental material suitable for developing of pulp-capping materials with osteogenic and bioactive properties.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 11","pages":"Pages 2043-2050"},"PeriodicalIF":4.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do all ceramic and composite CAD-CAM materials exhibit equal bonding properties to implant Ti-base materials? An Interfacial Fracture Toughness Study","authors":"Yousef Karevan ,&nbsp;Maher Eldafrawy ,&nbsp;Raphael Herman ,&nbsp;Christelle Sanchez ,&nbsp;Michaël Sadoun ,&nbsp;Amélie Mainjot","doi":"10.1016/j.dental.2024.07.014","DOIUrl":"10.1016/j.dental.2024.07.014","url":null,"abstract":"<div><h3>Objectives</h3><p>To compare the interfacial fracture toughness (IFT) with or without aging, of four different classes of CAD-CAM ceramic and composite materials bonded with self-adhesive resin cement to titanium alloy characteristic of implant abutments.</p></div><div><h3>Methods</h3><p>High translucent zirconia (Katana; KAT), lithium disilicate-based glass-ceramic (IPS. emax.CAD; EMX), polymer-infiltrated ceramic network material (PICN) (Vita Enamic; ENA), and dispersed filler composite (Cerasmart 270; CER) were cut into equilateral triangular prisms and bonded to titanium prisms with identical dimensions using Panavia SA Cement Universal. The surfaces were pretreated following the manufacturers’ recommendations and developed interfacial area ratio (Sdr) of the pretreated surfaces was measured. IFT was determined using the Notchless Triangular Prism test in a water bath at 36 °C before and after thermocycling (10,000 cycles) (n = 40 samples/material).</p></div><div><h3>Results</h3><p>IFT of the materials ranged from 0.80 ± 0.25 to 1.10 ± 0.21 MPa.m^1/2 before thermocycling and from 0.71 ± 0.24 to 1.02 ± 0.25 MPa.m^1/2 after thermocycling. There was a statistical difference between IFT of CER and the two top performers in each scenario: KAT and EMX before aging, and KAT and ENA after aging. Thermocycling significantly decreased IFT of EMX. The Weibull modulus of IFT was similar for all materials and remained so after thermocycling. Sdr measurements revealed that ENA (7.60)&gt;Ti (4.97)&gt;CER (2.85)&gt;KAT (1.09)=EMX (0.96).</p></div><div><h3>Significance</h3><p>Dispersed filler CAD-CAM composite showed lower performance than the other materials. Aging only affected IFT of Li-Si glass-ceramic, whereas zirconia and PICN performed equally well, probably due to their chemical bonding potential and surface roughness respectively.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1524-1533"},"PeriodicalIF":4.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of optical and numerical models for reflectance and color prediction of monolithic dental resin composites with varying thicknesses","authors":"Maria Tejada-Casado ,&nbsp;Vincent Duveiller ,&nbsp;Razvan Ghinea ,&nbsp;Arthur Gautheron ,&nbsp;Raphaël Clerc ,&nbsp;Jean-Pierre Salomon ,&nbsp;María del Mar Pérez ,&nbsp;Mathieu Hébert ,&nbsp;Luis Javier Herrera","doi":"10.1016/j.dental.2024.07.013","DOIUrl":"10.1016/j.dental.2024.07.013","url":null,"abstract":"<div><h3>Objective:</h3><p>To assess the prediction accuracy of recent optical and numerical models for the spectral reflectance and color of monolithic samples of dental materials with different thicknesses.</p></div><div><h3>Methods:</h3><p>Samples of dental resin composites of Aura Easy Flow (Ae1, Ae3 and Ae4 shades) and Estelite Universal Flow Super Low (A1, A2, A3, A3.5, A4 and A5 shades) with thicknesses between 0.3 and 1.8 mm, as well as Estelite Universal Flow Medium (A2, A3, OA2 and OA3 shades) with thicknesses between 0.4 and 2.0 mm, were used. Spectral reflectance and transmittance factors of all samples were measured using a X-Rite Color i7 spectrophotometer. Four analytical optical models (2 two-flux models and 2 four-flux models) and two numerical models (PCA-based and L*a*b*-based) were implemented to predict spectral reflectance of all samples and then convert them into CIE-L*a*b* color coordinates (D65 illuminant, 2°Observer). The CIEDE2000 total color difference formula (<span><math><mrow><mi>Δ</mi><msub><mrow><mi>E</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span>) between predicted and measured colors, and the corresponding 50:50% acceptability and perceptibility thresholds (<span><math><mrow><mi>A</mi><msub><mrow><mi>T</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span> and <span><math><mrow><mi>P</mi><msub><mrow><mi>T</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span>) were used for performance assessment.</p></div><div><h3>Results:</h3><p>The best performing optical model was the four-flux model RTE-4F-RT, with an average <span><math><mrow><mi>Δ</mi><msub><mrow><mi>E</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span> = 0.72 over all samples, 94.87% of the differences below <span><math><mrow><mi>A</mi><msub><mrow><mi>T</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span> and 65.38% below <span><math><mrow><mi>P</mi><msub><mrow><mi>T</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span>. The best performing numerical model was L*a*b*-PCHIP (interpolation mode), with an average <span><math><mrow><mi>Δ</mi><msub><mrow><mi>E</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span> = 0.48, and 100% and 79.69% of the differences below <span><math><mrow><mi>A</mi><msub><mrow><mi>T</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span> and <span><math><mrow><mi>P</mi><msub><mrow><mi>T</mi></mrow><mrow><mn>00</mn></mrow></msub></mrow></math></span>, respectively.</p></div><div><h3>Significance:</h3><p>Both optical and numerical models offer comparable color prediction accuracy, offering flexibility in model choice. These results help guide decision-making on prediction methods by clarifying their strengths and limitations.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1677-1684"},"PeriodicalIF":4.6,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002173/pdfft?md5=fe98bb596ea4b9f4e48964e656efe410&pid=1-s2.0-S0109564124002173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Release of contraction stress of dental resin composites by water sorption","authors":"Yasmin J. Feilzer ,&nbsp;Albert J. Feilzer ,&nbsp;Michael J. Noack ,&nbsp;Cornelis J. Kleverlaan","doi":"10.1016/j.dental.2024.08.001","DOIUrl":"10.1016/j.dental.2024.08.001","url":null,"abstract":"<div><p>Polymerization shrinkage of bonded resin composite restorations will result in the development of curing contraction stresses during setting and can cause debonding of the restoration or failure of the surrounding tooth structure. However, the hygroscopic expansion that occurs after exposure of the restorative to the wet oral environment can compensate for this shrinkage.</p></div><div><h3>Objectives</h3><p>The purpose of this study was to determine the hygroscopic expansion of six commercial resin composites and relate it to their composition, mechanical properties, shrinkage, and contraction stress development.</p></div><div><h3>Methods</h3><p>Short-term volumetric shrinkage and contraction stress of the different composites were measured by mercury dilatometry and a universal testing machine. The long-term contraction stress was measured by the deflection of a bilayer strip of metal and a resin composite, which were stored dry as well as wet to determine the effect of hygroscopic expansion. The curvature of the strip was measured by profilometry over a period of 3 months.</p></div><div><h3>Results</h3><p>The curvature of the strip correlated well (r² =0.74) with the initial contraction stress, showing that the contraction stress is an important factor in initial deformation. The water sorption in all specimens showed that the initial deformation, within 2–4 weeks after curing, was completely counteracted. A high correlation (r² =0.90) between deflection and relative water sorption was found, where the relative water sorption is defined as the absolute water sorption corrected for the inorganic filler volume of the composite.</p></div><div><h3>Significance</h3><p>Within a period of 2–4 weeks after curing most of the curing contraction stresses of resin composite restoratives will be released by hygroscopic expansion.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1697-1701"},"PeriodicalIF":4.6,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124002409/pdfft?md5=8dcdeba743d866ac682e6df87f2070da&pid=1-s2.0-S0109564124002409-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel co-initiators of polymerization: Cytotoxicity profile and modulation of inflammatory mediators in human dental pulp stem cells","authors":"Adriano F. Lima ,&nbsp;Giulia G. Pizzanelli ,&nbsp;Camila S. Stolf ,&nbsp;Jean-Pierre Salomon ,&nbsp;Jacques Lalevée ,&nbsp;Denise Carleto Andia","doi":"10.1016/j.dental.2024.07.018","DOIUrl":"10.1016/j.dental.2024.07.018","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to assess the cytotoxicity of novel polymerization co-initiators and their effect on cytokine release from human dental pulp stem cells (hDPSCs), comparing them with commonly used co-initiators.</p></div><div><h3>Methods</h3><p>Cells were isolated from the dental pulp of healthy human third molars. The new co-initiators, namely HDa1, HD4, HD1, and MHPTm, were evaluated and compared with the compounds dimethylaminoethyl amine benzoate (EDAB) and 2-(dimethylamino)ethyl methacrylate (DMAEMA). These compounds were diluted in dimethylsulfoxide (DMSO) at concentrations ranging from 1 to 8 mM. hDPSCs were seeded onto 96-well plates and incubated for 48 h. Subsequently, the cells were exposed to different concentrations of the co-initiators mentioned for 24 h. After this period, the culture medium was removed, and mitochondrial metabolism was evaluated using the MTT assay, while cytokine release (IL-1β, IL-6, IL-8, IL-10, TNF-α) was analyzed by the MAGPIX assay. Cells without exposure to the tested compounds served as controls. The data were analyzed using one-way ANOVA and Tukey’s test.</p></div><div><h3>Results</h3><p>The compounds showed low toxicity, with 8 mM concentration causing the most significant reduction in mitochondrial metabolism. MHPTm was the most toxic co-initiator tested (compound bearing an amine functionality). All compounds up-regulated TNF-α, IL-10, IL-6, and IL-8, with HD4 exhibiting the most pronounced increase in IL-6 and IL-8.</p></div><div><h3>Significance</h3><p>The newly proposed co-initiators demonstrated reduced impact on mitochondrial metabolism, comparable to some traditional co-initiators. Despite their lower toxicity, HD4 increased IL-6 and IL-8 release, suggesting its potential involvement in triggering an inflammatory reaction, particularly in the short term.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1692-1696"},"PeriodicalIF":4.6,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of adding ytterbium trifluoride filler particles on the mechanical, physicochemical and biological properties of methacrylate-based experimental resins for 3D printing","authors":"Emanuela Gaviolli ,&nbsp;Fabricio Mezzomo Collares ,&nbsp;Gabriela de Souza Balbinot ,&nbsp;Mutlu Özcan ,&nbsp;Vicente Castelo Branco Leitune","doi":"10.1016/j.dental.2024.07.023","DOIUrl":"10.1016/j.dental.2024.07.023","url":null,"abstract":"<div><h3>Objective</h3><p>To formulate an experimental methacrylate-based photo-polymerizable resin for 3D printing with ytterbium trifluoride as filler and to evaluate the mechanical, physicochemical, and biological properties.</p></div><div><h3>Methods</h3><p>Resin matrix was formulated with 60 wt% UDMA, 40 wt% TEGDMA, 1 wt% TPO, and 0.01 wt% BHT. Ytterbium Trifluoride was added in concentrations of 1 (G_1 %), 2 (G_2 %), 3 (G_3 %), 4 (G_4 %), and 5 (G_5 %) wt%. One group remained without filler addition as control (G_C). The samples were designed in 3D builder software and printed using a UV-DLP 3D printer. The samples were ultrasonicated with isopropanol and UV cured for 60 min. The resins were tested for degree of conversion (DC), flexural strength, Knoop microhardness, softening in solvent, radiopacity, colorimetric analysis, and cytotoxicity (MTT and SRB).</p></div><div><h3>Results</h3><p>Post-polymerization increased the degree of conversion of all groups (p &lt; 0.05). G_2 % showed the highest DC after post-polymerization. G_2 % showed no differences in flexural strength from the G_1 % and G_C (p &gt; 0.05). All groups showed a hardness reduction after solvent immersion. No statistical difference was found in radiopacity, softening in solvent (ΔKHN%), colorimetric spectrophotometry, and cytotoxicity (MTT) (p &gt; 0.05). G_1 % showed reduced cell viability for SRB assay (p &lt; 0.05).</p></div><div><h3>Significance</h3><p>It was possible to produce an experimental photo-polymerizable 3D printable resin with the addition of 2 % ytterbium trifluoride as filler without compromising the mechanical, physicochemical, and biological properties, comparable to the current provisional materials.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 10","pages":"Pages 1685-1691"},"PeriodicalIF":4.6,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141878065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}