Asian Journal of Natural Product Biochemistry最新文献

{"title":"Comparative phytochemical analysis of mature mango leaves from nineteen cultivars of Murshidabad district, India","authors":"B. Ghosh, Soumya Majumder, S. Acharyya, A. Ghosh, S. Saha, S. Sarkar, S. Chakraborty, M. Bhattacharya","doi":"10.13057/biofar/f200202","DOIUrl":"https://doi.org/10.13057/biofar/f200202","url":null,"abstract":"Abstract. Ghosh B, Majumder S, Acharyya S, Ghosh A, Saha S, Sarkar S, Chakraborty S, Bhattacharya M. 2022. Comparative phytochemical analysis of mature mango leaves from nineteen cultivars of Murshidabad district, India. Asian J Nat Prod Biochem 20: 48-55. Mango (Mangifera indica L.), \"the king of fruits,\" is one of the most popular fruits in tropical regions. This research aimed to qualitatively and quantitatively screen major phytochemical groups present in the leaves of nineteen cultivars of mango tree extracted with three different solvents (petroleum benzene, acetone, methanol) belonging to different polarities and to determine their antibacterial activity against two Gram-positive (Bacillus subtilis, Staphylococcus aureus) and two Gram-negative (Escherichia coli, Klebsiella pneumoniae) bacteria. The antiradical scavenging activity was performed using a DPPH assay. In addition, total phenol and flavonoids in the leaf extracts were also quantitatively analyzed. Phytochemical investigations showed that mango leaf extracts contain phenol, tannin, protein, coumarin, terpenoid, alkaloid, steroid, and cardiac glycoside. The extracts also contain a variety of total phenols and flavonoids. Antiradical scavenging activity showed that polar solvents (methanol, acetone) are more potent than non-polar (petroleum benzene) extracts. Mango leaf extracts inhibit the growth of E. coli, K. pneumoniae, and S. aureus, but no inhibition zone against B. subtilis. Based on the phytochemical compounds and significant antioxidant and antibacterial properties of mango leaf extracts, mango leaves might be a potential source for developing pharmaceutical formulations and drugs.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"35 13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131480207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical screening and antioxidant activity of Ipomoea hederifolia stems: A potential medicinal plant","authors":"Mahmud Hossain, Mohammad Sarowar Uddin, P. Baral, Mahmuda Ferdus, S. Bhowmik","doi":"10.13057/biofar/f200201","DOIUrl":"https://doi.org/10.13057/biofar/f200201","url":null,"abstract":"Abstract. Hossain MM, Uddin MS, Baral PK, Ferdus M, Bhowmik S. 2022. Phytochemical screening and antioxidant activity of Ipomoea hederifolia stems: A potential medicinal plant. Asian J Nat Prod Biochem 20: 41-47. Ipomoea hederifolia L., a plant of Convolvulaceae family, popularly known as morning glory, possesses numerous medicinal values. The present study aimed to explore the antioxidant activity and bioactive compounds of I. hederifolia stems (IHS). The IHS was soaked in methanol for 21 days. The filtrate was concentrated using rotary evaporator after filtration to obtain IHS extract which is subjected to phytochemical screening using various tests. Antioxidant activity was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and reducing power activity (RPA) methods. The phytochemical screenings revealed that IHS extract possesses carbohydrates, tannins, flavonoids, phenols, saponin, alkaloids, steroids, anthraquinones, and cardiac glycosides. The antioxidant activity of IHS extract in DPPH model was moderate (IC50 :174.08 µg/ml) in comparison with standards (IC50: 102.28 µg/mL AA; IC50: 88.52 µg/mL for BHT). It indicates that IHS exerts free radical scavenging power in dose dependent fashion. The RPA model also produced moderate antioxidant potential (EC50: 279.58 µg/mL for IHS; EC50: 23.121 µg/mL for AA; EC50: 50.84 µg/mL for BHT) depending on increasing order of dose. Based on the findings of this investigation, we can conclude that IHS extract possesses various bioactive compounds and moderate antioxidant potentials which may be path to discovery of traditional medicines and remedies for many critical diseases.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128239681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, purification, and quantification of hesperidin from the immature Citrus grandis/maxima fruit Nepal cultivar","authors":"H. K. Manikyam, Prathibha Tripathi, S. Patil, Janardan Lamichhane, M. Chaitanya, A. Patil","doi":"10.13057/biofar/f200105","DOIUrl":"https://doi.org/10.13057/biofar/f200105","url":null,"abstract":"Abstract. Manikyam HK, Tripathi P, Patil SB, Lamichhane J, Chaitanya MVNL, Patil AR. 2022. Extraction, purification, and quantification of hesperidin from the immature Citrus grandis/maxima fruit Nepal cultivar. Asian J Nat Prod Biochem 20: 21-26. Hesperidin, a flavanone group of flavonoids and a 7-O-rutinoside of hesperitin is abundantly present in citrus fruits. Pomelo or Citrus grandis (L.) Osbeck/ C. maxima (Burm.) Merr. is grown and cultivated in Nepal as seasonal edible fruit. Pomelo has flavanone and other chemical constituents. Flavanones like naringin, hesperidin, neohesperidin, and naringenin are present in the fruits of C. grandis. Many extraction techniques have been reported in the extraction and purification of hesperidin citrus fruits like sweet lemons, grapefruits, mandarins, etc. However, little information has been provided on hesperidin content in Pomelo fruits. In order to estimate the quantity of hesperidin in the Pomelo fruits of Nepal, we have first time made study. The immature Pomelo fruits of size 2-7 cm were collected from the cultivation field and dried completely. The pulverized fruit powder with moisture content less than 15% was subjected to 5% methanolic acetic extraction and Dimethylformamide (DMF) solvent extraction in Soxhlet apparatus by refluxing at 90°C in 1:15 ration in three subsequent extractions of each 1 hour. The concentrates were crystalized at freezing temperatures in methanol and further purified by repeating the crystallization process. The white amorphous powder was subjected to RP-HPLC analysis method, chromatographic conditions 285 nm wavelength, C18 column 4.6 mm x 15 cm x 5 µm, flow rate 1.2 mL/minute with injection volume 10 µL run time of 30 minutes, column temperature 40°C. Hesperidin yields were 0.12 g/100 g with acetic acid methanol with a purity of 87% and 0.17 g/100 g in DMF extraction with a purity of 90% with recoveries of 87% in acetic acid methanol extraction and 90% in DMF extraction when compared with standard 93%. Further extraction does not yield any hesperidin content indicating a total of 0.15-0.17 g/100 g of hesperidin content in the whole immature fruit of C. grandis/maxima. Thus we found DMF and 5% methanolic acetic solvents as preferable solvents to extract hesperidin.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128659514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short Communication: Extraction and characterization of pectin from ripe and unripe mango (Mangifera indica) peel","authors":"Christopher Shaibu, Julius Dinshiya, Vivian Ebere Shaibu","doi":"10.13057/biofar/f200104","DOIUrl":"https://doi.org/10.13057/biofar/f200104","url":null,"abstract":"Abstract. Shaibu CO, Dinshiya J, Shaibu VE. 2022. Short Communication: Extraction and characterization of pectin from ripe and unripe mango (Mangifera indica) peel. Asian J Nat Prod Biochem 20: 16-20. Pectin is a heteropolysaccharide present in the cell walls of different plants at different concentrations with widespread applications. This work aimed at extracting and characterizing pectin from ripe and unripe mango peel to investigate the effect of ripeness on the yield and quality of mango pectin. To obtain the optimum extraction condition, pectin was extracted at varying temperatures, time and pH using 0.1 N HCl as the extraction solvent. The maximum yield of pectin was found to be 22.67% for ripe mango peel and 21.90% for unripe mango peel. The optimum extraction conditions were found to be 90ºC, 60mins and pH 1.5. The pectin extracted using the optimum extraction conditions was then characterized. The moisture content, ash content, methoxyl content, equivalent weight, anhydrouronic acid and degree of esterification of the ripe mango peel pectin were found to be 8.76±0.08%, 10.12±0.47%, 9.17±0.27%, 883.07±13.85 g, 72.45±0.59 and 72.52±0.09% , respectively. In contrast, those of unripe mango peel pectin were found to be 8.13±0.13%, 9.12±0.34%, 8.83±0.19%, 823.38±14.07 g, and 71.56±0.34%, 70.34±0.38%, respectively. This study showed that ripe mango peel might be more suitable for use as a pectin source. However, pectin extracted from ripe and unripe mango peel could be considered an alternative source of pectin in food processing, pharmaceutical industries, and various places of pectin application.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121287420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of palm sap (Arenga pinnata) on blood glucose levels of mice (Mus musculus) alloxan-induced diabetic","authors":"Sindi Maryam Liputo, D. Lamondo, M. Solang","doi":"10.13057/biofar/f200103","DOIUrl":"https://doi.org/10.13057/biofar/f200103","url":null,"abstract":"Abstract. Liputo SM, Lamondo D, Solang M. 2021. Effect of palm sap (Arenga pinnata) on blood glucose levels of mice (Mus musculus) alloxan-induced diabetic. Asian J Nat Prod Biochem 20: 11-15. This study aims to determine the effect of palm sap (Arenga pinnata (Wurmb) Merr.) in reducing blood glucose levels in alloxan-induced diabetic mice (Mus musculus Linnaeus, 1758). Twenty-five mice aged 2-3 months were grouped into a negative control group, a positive control group and a palm sap group (0.2 mL, 0.4 mL, and 0.6 mL). The result showed that the palm sap with a dose of 0.4 mL was the most significant in reducing blood glucose levels. This study concluded that the administration of palm sap affected decreasing blood glucose levels (p <0.05).","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130073130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of phytochemical constituents of ethanol leaf extracts of Solanum macrocarpon and Vernonia amygdalina","authors":"O. Oyesola, Iquot Isaac Sampson, Adebiyi Adelowo Augustine, Olukade Baliqis Adejoke, G. Taiwo","doi":"10.13057/biofar/f200102","DOIUrl":"https://doi.org/10.13057/biofar/f200102","url":null,"abstract":"Abstract. Oyesola OA, Sampson II, Augustine AA, Adejoke OB, Taiwo GE. 2022. Comparison of phytochemical constituents of ethanol leaf extracts of Solanum macrocarpon and Vernonia amygdalina. Asian J Trop Biotechnol 19: 6-10. A plant can exert physiological changes on biological systems due to phytochemicals present in plants parts. This study evaluated and compared the phytochemical constituent (both quantitative and qualitative) and the antioxidant properties of the ethanol leaf extract Solanum macrocarpon L.and Vernonia amygdalina Delile using standard methods. Tannin, phenol, cardiac glycoside, alkaloid, and flavonoid were present in both plant extracts. Alkaloid, flavonoid, and cardiac glycoside in large quantities in S. macrocarpon compared to V. amygdalina. At the same time, V. amygdalina contained a large amount of tannin and phenol. Reducing sugar was present in S. macrocarpon and absent in V. amygdalina, saponin and steroid were present in V. amygdalina and absent in S. marcrocarpon, terpenoid and phlobatanin were both missing in the leaf extract of both plants. In contrast, both plant extracts showed radical scavenging activities, with V. amygdalina having a higher antioxidant capacity than S. macrocarpon. The present study's findings indicated that the ethanol leaf extract of S. macrocarpon and V. amygdalina possess antioxidant properties and may be effective against oxidative stress.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122331743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosafety evaluation of ethanolic extract of Phyllanthus amarus leaves on liver and kidney of wistar rats","authors":"O. Yakubu, Michael Sunday Abu, Okonkwo Chibuike Innocent, Job Itanyi Onuche","doi":"10.13057/biofar/f200101","DOIUrl":"https://doi.org/10.13057/biofar/f200101","url":null,"abstract":"Abstract. Yakubu OE, Abu MS, Innocent OC, Onuche JI. 2022. Biosafety evaluation of ethanolic extract of Phyllanthus amarus leaves on liver and kidney of wistar rats. Asian J Nat Prod Biochem 20: 1-5. Medicinal plants such as Phyllanthus amarus Schumach. & Thonn. have played a significant role in maintaining human health since ancient times. Documented evidence shows that the plant has been used to treat various ailments. Still, its biosafety on vital organs such as the liver and kidney has not been specifically studied. The study was designed to ascertain the biosafety of ethanolic extract of P. amarus leaves on the liver and kidney of wistar rats. A total of twenty (20) wistar rats were used, divided into 4 groups of 5 animals each (Group A, B, C, D). Group B, C, D received 100 mg/kg, 200 mg/kg, 400 mg/kg of the extract, respectively. Group A (Control) received only distilled water. The treatment was carried out for two weeks. At the end of the experiments, the animals were sacrificed. Afterward, liver function and renal function evaluation were conducted. The indicators of liver function include Total protein (TP), Aspartate aminotransferase (AST), Alkaline Phosphatase (ALP), Alanine aminotransferase (ALT), Albumin (ALB), Direct bilirubin (DB), and total bilirubin (TB) were determined. The indicators of renal function include sodium ion (Na+), potassium ion (k+), Chlorine ion (Cl-), urea, and creatinine. The results showed that the parameters of the liver function of treated animals were no significant (p>0.0) difference compared to the normal rats (control). Concentrations of sodium ions (Na+), potassium ions (k+), chlorine ions (Cl-), urea, and creatinine of the treated animals were statistically comparable to the normal rats at (p<0.05). This research showed that P. amarus did not induce hepatotoxicity or nephrotoxicity at the dose of 100-400 mg/kg BW. Furthermore, this finding has validated the use of P. amarus as a traditional herb for treating and managing several diseases, including kidney and liver diseases, without any significant organ damage.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131042659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative determination of total antioxidant effects of ethanol extract of Phyllanthus amarus leaves","authors":"O. Yakubu, Michael Sunday Abu, John Akighir, Job Itanyi Onuche, Ali M E Arabi","doi":"10.13057/biofar/f190206","DOIUrl":"https://doi.org/10.13057/biofar/f190206","url":null,"abstract":"Abstract. Yakubu OE, Abu MS, Akighir J, Onuche JI, Arabi A. 2021. Comparative determination of total antioxidant effects of ethanol extract of Phyllanthus amarus leaves. Asian J Nat Prod Biochem 19: 81-85. Some medicinal and therapeutic potency have been reported of Phyllanthus in traditional settings. Hence, the current study evaluated the total antioxidant potential, phenolics, and flavonoids of fractions of ethanol extract of Phyllanthus amarus leave obtained using column chromatography. Extraction was done using absolute ethanol; elution was performed using different organic solvents of varying polarities, from n-Hexane, Chloroform, Ethylacetate, Ethanol, Methanol, and finally, water. The results obtained from the study revealed that fraction 10b (83 mg/mL) has the highest concentration of total Antioxidant Capacity (TAC), the next being 1a, 5a, and 11b (48mg/mL). The reduction in the TAC of the fractions was in the order; of 3b>7a>6a>5b>4a> with F7b (13mg/mL) having the lowest TAC. The result for Total phenolic Content (TPC) shows that fraction 10b (430 mg/mL) has the highest concentration of TPC, just like in antioxidant activity, followed by F4b (428 mg/mL). Fraction 2a (9 mg/mL) has the lowest concentration of TPC. Total Flavonoid Content (TFC) showed that fraction 3b (201 mg/mL) has the most concentration of total flavonoids, followed by 4b (120mg/mL). Meanwhile, fractions 1a, 2b, and 9a have the same TFC. While fraction 2a showed the lowest flavonoid concentration (25mg/mL). The plant extract fractions were found to contain more both phenolics and flavonoids, which may have contributed to the antioxidant activity observed.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128020482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical composition of some new craft beers consumed in Maroua town from Far North region of Cameroon","authors":"M. Diguir, A. Laya, H. Wangso, J. P. Bayang, B. Koubala","doi":"10.13057/biofar/f190205","DOIUrl":"https://doi.org/10.13057/biofar/f190205","url":null,"abstract":"Abstract. Diguir M, Laya A, Wangso H, Bayang JP, Koubala BB. 2021. Physicochemical composition of some new craft beers consumed in Maroua town from Far North region of Cameroon. Biofarmasi J Nat Prod Biochem 19: 70-80. The work aimed to investigate the physicochemical composition of various craft beers (CB) and their distillates produced in Maroua town. The beers named “Bil-Bil”,“Cochette” and “Furdu\" were collected both morning and evening time. Dry matter, pH, titratable acid, total dissolved solids, proteins, sugars, carbohydrates, amino acids and alcohol content were evaluated. The results showed that local beers had a pH ranged from 4.06 to 4.22, 4.15 to 3.84 and 3.53 to 3.37 for \"Furdu\", \"Cochette\" and “Bil-bil”, respectively. The alcohol varied between 5.40 and 6.92%, 1.08 and 5.10%, 1.73 and 2.88 for “Furdu”, “Cochette\" and \"Bil-bil\", respectively. The sugars ranged from 11.85 to 19.05 mg/mL for “Furdu”, from 2.20 to 9.46 mg/mL for “Cochette” and from 9.92 to 19.78 mg/mL for \"Bil-bil\". For amino acids, the values varied from 3.40 to 7.37 mg /mL for “Furdu”, 3.98 to 6.01 mg / mL for “Cochette” and 2.95 to 3.24 mg/mL for “Bil-Bil”. Regarding protein, the values ranged from 0.80 to 0.85 mg/mL for these three CB. The distillates of CB collected evening showed high alcohol in fraction 1. Thus, these CB analysed can be promoted for alternative beer in Maroua town. Furthermore, the unsold CB can be distilled into ethanol.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132280839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography","authors":"Yusfia Urwatul Wutsqa, S. Suratman, S. Sari","doi":"10.13057/biofar/f190204","DOIUrl":"https://doi.org/10.13057/biofar/f190204","url":null,"abstract":"Abstract. Wutsqa YU, Suratman, Sari SA. 2021. Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography. Biofarmasi J Nat Prod Biochem 19: 65-69. The diversity of ferns on this earth is very high, and the potential and benefits for human life are ornamental plants, food materials, and medicines. The benefits of not all ferns are known because of the lack of information on these ferns, one of the ferns is Lindsaea obtusa J. Sm. Ex Hook. The phytochemical test is the first step in research, searching for new active compounds derived from natural ingredients. This study was conducted to determine the differences of terpenoid and steroid compounds profile in L. obtusa extract using Thin Layer Chromatography (TLC). Extraction of L. obtusa using maceration techniques with methanol and n-hexane solvents. The extraction results will be used for phytochemical screening using the TLC technique with GF254 silica gel plate as a stationary phase, and a mobile phase is chloroform: n-hexane (9: 1 v/v), n-hexane: ethyl acetate (4: 1 v/v), and methanol: ethyl acetate (5: 1 v/v). Detection of terpenoid and steroids using Lieberman Burchard reagent. The result shows that L. obtusa extract contains terpenoid and steroid compounds. Quantitatively, the secondary metabolites of L. obtusa extract were soluble in methanol solvent more than the soluble compounds in n-hexane solvent. However, qualitatively, steroid compounds that dissolve in n-hexane solvents have a more varied Rf value than terpenoids, especially in the mobile phase of chloroform: n-hexane (9: 1 v/v) and n-hexane: ethyl acetate (4: 1 v/v). L. obtusa contains more various steroid compounds than terpenoid compounds.","PeriodicalId":293159,"journal":{"name":"Asian Journal of Natural Product Biochemistry","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124038785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}