Koomesh journal最新文献_第9页

Evaluation of the epidemiological pattern of COVID-19 applying basic reproduction number: An educational review article 应用基本复制数评价COVID-19流行病学模式:一篇教育综述文章

Koomesh journal Pub Date : 2020-05-01 DOI: 10.29252/koomesh.22.3.373

M. Mirmohammadkhani, Fatemeh Paknazar, A. Rashidy-Pour

引用次数: 1

Association of low-carbohydrate diet score with perceived stress score: A cross-sectional study in Iranian nurses 低碳水化合物饮食评分与感知压力评分的关联:伊朗护士的横断面研究

Koomesh journal Pub Date : 2020-05-01 DOI: 10.29252/koomesh.22.3.452

A. Dehghani, M. Sanjari, S. Salemi, Z. Maghbooli, K. Mirzaie

引用次数: 1

Effects of ackA, pta and poxB inhibition by antisense RNA on acetate excretion and recombinant beta interferon expression in Escherichia coli 反义RNA抑制ackA、pta和poxB对大肠杆菌醋酸酯分泌和重组β干扰素表达的影响

Koomesh journal Pub Date : 2020-05-01 DOI: 10.29252/KOOMESH.22.3.505

Mohammad Hossein Morowvat

{"title":"Effects of ackA, pta and poxB inhibition by antisense RNA on acetate excretion and recombinant beta interferon expression in Escherichia coli","authors":"Mohammad Hossein Morowvat","doi":"10.29252/KOOMESH.22.3.505","DOIUrl":"https://doi.org/10.29252/KOOMESH.22.3.505","url":null,"abstract":"Introduction : Escherichia coli (E.coli) is one of the most widely used hosts for the production of recombinant proteins. The main problem in getting high product yields and productivity is the accumulation of acetic acid (acetate) as an unwanted metabolic by-product. In this study, an antisense-based strategy as a metabolic engineering approach was employed to hamper the acetate excretion problem. Materials and Methods : A recombinant plasmid containing the encoding genes for human recombinant interferon beta (rhINF-β) and three antisense oligonucleotides against acetate kinase, phosphotransacetylase and pyruvate oxidase B was designed. The effects of recombinant plasmid on the cell physiology, rhINF-β production and acetate excretion were studied in E. coli. Results: The mRNA levels of the targeted enzymes were lowered in antisense-regulated cells compared to the control cells. The concentration of acetate in culture media was decreased due to the constructed plasmid. The expression of antisense RNA did not affect the cell growth, negatively. Besides, the rhINF-β production was enhanced in antisense-regulated strain compared to the control plasmid without antisense genes. Conclusion: Application of an antisense strategy on the acetate pathway was successful in metabolically engineering E. coli. This enhancement of production yield by antisense technology suggests that this strategy may be successfully applied to high cell density fermentations of E. coli expression system to overexpress other recombinant proteins.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126383678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of global Spata19 knockout mouse using CRISPR/Cas9 nickase technology 利用CRISPR/Cas9缺口酶技术生成Spata19基因敲除小鼠

Koomesh journal Pub Date : 2020-05-01 DOI: 10.29252/koomesh.22.3.380

Mahsa Zargar, Abbas Jamshidizad, Aidin Rahim-Tayefeh, Ehsan Hashemi, A. Najafi, M. Shamsara, M. Modarressi

{"title":"Generation of global Spata19 knockout mouse using CRISPR/Cas9 nickase technology","authors":"Mahsa Zargar, Abbas Jamshidizad, Aidin Rahim-Tayefeh, Ehsan Hashemi, A. Najafi, M. Shamsara, M. Modarressi","doi":"10.29252/koomesh.22.3.380","DOIUrl":"https://doi.org/10.29252/koomesh.22.3.380","url":null,"abstract":"Introduction : SPATA19 gene is expressed in developmental stages of testis and some organs, but so far its function has only been examined in the testis. In this study, we provided an effective pathway for the generation of these mice using new CRISPR / Cas9 nickase method while generating Spata19 knockout mice for future studies in other organs. Materials and Methods : CRISPR / Cas9 nickase plasmids were synthesized using pX335 vector and designed gRNAs. So, each plasmid was transformed into E.coli and clonal selection was performed. The plasmids extracted from the bacteria were mixed in equal proportions and injected into the male pronucleus. Correspondingly, the zygotes carrying the plasmid were transferred to Oviduct of Foster mice. After pregnancy extent, born mice were analyzed. Results: We generated global knockout mice using CRISPR/Cas9 nickase technology. This deletion was examined at three levels of DNA, RNA and protein. Generated 2 nucleotides deletion was confirmed by sequencing at three levels of DNA, RNA and protein. By translating the mutated RNA sequence, it was determined that this deletion could cause premature stop codon. Phenotypic analysis confirmed infertility of male homozygous mice (Spata19 -/-). Conclusion: By generating Spata19 knockout mice, we present a protocol for generation of knockout mice. These mice could be applied as the infertile male mice and also for further characterization of Spata19 gene.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"7 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131382737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparing the effectiveness of cognitive-behavioral art-play and cognitive-behavioral story therapy on health related quality of life among children with cancer 比较认知行为艺术游戏与认知行为故事治疗对癌症儿童健康相关生活质量的影响

Koomesh journal Pub Date : 2020-04-01 DOI: 10.29252/KOOMESH.22.2.244

Zohreh Shojaei, M. Golparvar, Asghar ------- Aghaei, M. Bordbar

{"title":"Comparing the effectiveness of cognitive-behavioral art-play and cognitive-behavioral story therapy on health related quality of life among children with cancer","authors":"Zohreh Shojaei, M. Golparvar, Asghar ------- Aghaei, M. Bordbar","doi":"10.29252/KOOMESH.22.2.244","DOIUrl":"https://doi.org/10.29252/KOOMESH.22.2.244","url":null,"abstract":"Introduction : Children with cancer need psychological treatment to increase their quality of life along with medical treatments. Accordingly, the aim of this study was to determine the comparing the effectiveness of cognitive-behavioral art-play therapy group and the cognitive-behavioral story therapy on health related quality of life among children with cancer. Materials and Methods : The research method was semi-experimental, and research design was three groups with two stages (pre-test and post-test design). From statistical population of 8-12 years old children with cancer in Amir Hospital (Shiraz city, Iran) in the Summer of 2018, 36 children were selected and then randomly assigned in two therapeutic groups (each group with 12 cases) and a control group (with 12 cases). Children Health related Quality of Life Scale (KiD-KINDL-Bullinger & Ravens-Sieberer, 1998) was used to measure the dependent variable in the pretest and post-test. The group of cognitive-behavioral art-play therapy and cognitive-behavioral story therapy received 12 sessions of treatment. The data were analyzed using multivariate analysis of covariance. Results: The results showed that there was a significant difference between cognitive-behavioral art-play therapy and cognitive-behavioral story therapy with the control group in health related quality of life (P<0.001) and their components. However, there was no significant difference between cognitive-behavioral art-play therapy and cognitive-behavioral story therapy in increasing the health related quality of life in children with cancer (P>0.05). Conclusion: It is suggested that cognitive-behavioral story therapy and cognitive-behavioral art-play therapy should be used in educational and therapeutic environments to promote the level of health related quality of life for children with cancer.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114799485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Efect of a controlled diet program on behavioral-emotional disorders in patients with phenylketonuria 控制饮食计划对苯丙酮尿患者行为情绪障碍的影响

Koomesh journal Pub Date : 2020-04-01 DOI: 10.29252/koomesh.22.2.317

Javad Shahpouri, S. Karami, Davood Mohammadi, Mohsen Mollamohammadi, A. Mohammadbeigi

{"title":"Efect of a controlled diet program on behavioral-emotional disorders in patients with phenylketonuria","authors":"Javad Shahpouri, S. Karami, Davood Mohammadi, Mohsen Mollamohammadi, A. Mohammadbeigi","doi":"10.29252/koomesh.22.2.317","DOIUrl":"https://doi.org/10.29252/koomesh.22.2.317","url":null,"abstract":"to deficiency of the phenylalanine hydroxylase enzyme. or untreatement would cause impacts on motor-cognitive and individual-social skills. This study aimed to investigate the effect of a controlled program on behavioral-emotional disorders in patients with phenylketonuria. Materials and Methods : In a quasi-experimental study, 60 patients with phenylketonuria referred to Hazrate Masoumeh Hospital in Qom (the seventh largest city in Iran) were studied. The data were collected using parents' strengths and difficulties questionnaire (SDQ) to assess the status of behavioral-emotional disorders in patients by one of parents. Data were collected before and after - a three-months controlled diet- intervention. Results: The mean of phenylalanine in the patients was 12.81 ± 5.89 mg/dl before intervention and was decreased to 11.88 ± 6.8 mg/dl after intervention and this difference was statistically significant ( p =0.018). The most significant effect of intervention was on increase of prosocial behaviors equal to 45%, reduction of conduct problems 38.3%, reduction of hyperactivity-attention deficit problems by 35%, decrease of emotional symptoms by 35% ( p <0.001). The mean score of the questionnaire and its sub-scales including emotional symptoms, conduct and hyperactivity problems decreased significantly after intervention (p<0.001), and prosocial behaviors showed a significant increase ( p <0.001). Conclusion: A three-months regular and controlled nutritional intervention showed a positive effect on behavioral-emotional disorders in patients with phenylketonuria. The diet intervention reduces patients' problems in sub-scales of emotional symptoms, behavioral, and hyperactivity problems and promote the prosocial behaviors.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121864731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of maximal and sub-maximal resistance exercise on muscle damage, inflammation, intrinsic antioxidant in non-athlete men 最大和次最大阻力运动对非运动员男性肌肉损伤、炎症和内在抗氧化的影响

Koomesh journal Pub Date : 2020-04-01 DOI: 10.29252/KOOMESH.22.2.351

A. P. Ghouroghchi, Mehdi Pahlevani

{"title":"Effects of maximal and sub-maximal resistance exercise on muscle damage, inflammation, intrinsic antioxidant in non-athlete men","authors":"A. P. Ghouroghchi, Mehdi Pahlevani","doi":"10.29252/KOOMESH.22.2.351","DOIUrl":"https://doi.org/10.29252/KOOMESH.22.2.351","url":null,"abstract":"Introduction : The intensity of the strength training at the start for preventing muscle damage, inflammation and intrinsic antioxidant is not well defined. Intrestingly, the purpose of this study was to evaluate the effect of maximal and sub-maximal resistance exercise on muscle damage, inflammation and intrinsic antioxidant in non-athlete men. Materials and Methods : Nineteen young untrained men [(age (years):20.16 ± 1.21 years, height (cm):178.21 ± 1.75, weight(kg): 65.74 ± 1.37] completed 3sets,15 repetitions, resistance exercise with two maximal and submaximal intensities with 3 minutes' intervals. Blood samples were obtained at pre-exercise, immediately, 2h, 24h, 48h and 72h after exercise. Correspondingly, blood samples were analyzed for creatin kinase (CK), total leucocytes and uric acid (UA). Results: CK increased significantly 48h and 72h after both resistance training intensities compared to base values. The increase of this enzyme was significantly higher 72h after maximal intensity resistance training compared to submaximal intensity. Considerably, leuckocyte increased significantly 24h, 48h and 72h after both resistance training intensitis compared to base values, but it was significantly decreased 2h after both intensity resistance training compared to before and immediately after exercise. Uric acid increased significantly 2h, 24h and 72h after high resistance training intensity and 2h, 48h and 72h after submaximal resistance training intensity compared to base values. Whereas it was significantly decreased 48h after maximal intensity compare to submaximal intensity (P<0.05). Conclusion: It is recommended that untrained individuals and trainer start resistance training with submaximal intensity for preventing muscular damage and maintaining health.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130720958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Quality of life in patients with hypertension and type 2 diabetes mellitus 高血压合并2型糖尿病患者的生活质量

Koomesh journal Pub Date : 2020-04-01 DOI: 10.29252/koomesh.22.2.263

Nemat Sotodeh Asl, Melika Avazabadian, R. Ghorbani, F. Malek

{"title":"Quality of life in patients with hypertension and type 2 diabetes mellitus","authors":"Nemat Sotodeh Asl, Melika Avazabadian, R. Ghorbani, F. Malek","doi":"10.29252/koomesh.22.2.263","DOIUrl":"https://doi.org/10.29252/koomesh.22.2.263","url":null,"abstract":"Introduction : the of of pressure leads to planning and measures to prevent or control complications and improve the quality of life of patients. The aim of this study was to evaluate the quality of life of patients with hypertension and diabetes types Materials and Methods : This descriptive cross-sectional study was performed on 150 subjects of 100 patients (50 type 2 diabetes and 50 hypertension) and 50 healthy individuals from attending patients (lack of diabetes, hypertension and other chronic diseases according to a clinical expert) who had been referred to the clinic at Kosar Hospital (Semnan, Iran) during the first six months of 2017. The data collection tool was a personal characteristics and disease list and a quality of life questionnaire (SF-36). Results: There were no significant differences in age group, marital status, education and place of residence. The groups were significantly different in terms of overall quality of life score ( p =0.013) and in physical dimensions ( p =0.001) and psychological symptoms ( p =0.013). So that the overall quality of life score of diabetic patients was less likely to have hypertensive patients ( p =0.009). Remarkably, the quality of life of patients with type 2 diabetes was lower in the subscales of physical and psychological symptoms in patients with high blood pressure (P<0.001). Conclusion: Findings showed that the quality of life score of patients with diabetes was lower than the patients with hypertension, especially in physical and psychological symptoms. Therefore, planning to improve the quality of life of patients with diabetes is necessary.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124100310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of mother's infant massage on neonatal weight and perceived self-efficacy of mothers with low birth weight infants 母婴按摩对低出生体重儿母亲新生儿体重及自我效能感的影响

Koomesh journal Pub Date : 2020-04-01 DOI: 10.29252/koomesh.22.2.237

S. Mousavi, S. Rostami, H. Haghani, L. Borimnejad

{"title":"Effect of mother's infant massage on neonatal weight and perceived self-efficacy of mothers with low birth weight infants","authors":"S. Mousavi, S. Rostami, H. Haghani, L. Borimnejad","doi":"10.29252/koomesh.22.2.237","DOIUrl":"https://doi.org/10.29252/koomesh.22.2.237","url":null,"abstract":"Introduction : Weight is the most important growth factor in newborns and perceived self-efficacy is known as an effective factor weigh gaining. The aim of this study was to determine the effect of mother's infant massage on neonatal weight and perceived self-efficacy of mothers with low birth weight infants. Materials and Methods : 80 infants were divided into intervention and control groups in this qusiexperimental study in neonatal intensive care unit. 40 low-birth weight infants received massage by their mother for 60 days, 15 minutes a day, and only normal care was taken in the control group. At the end of the study, weight gain and self-efficacy scores were analyzed standard statisctical methods. Results: The results showed that there was a significant difference between the mean weight changes in the intervention group (2120.88± 385.26) and control (1760.88 ± 220.31) at the tenth day of birth and two months (P = 0.001). The results of independent t-test showed a significant increase in self-efficacy scores and its dimensions in the two intervention groups compared to the control group (P<0.001). Conclusion: Low-birth-weight infant massage improves weight gain and promotes perceived self-efficacy in mothers. Due to the low cost of this method, it is suggested to teach massage techniques as one of the essential training during discharge in newborns.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116391616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of 12 weeks of combined aerobic-resistance exercise training on levels of chemerin, omentin and insulin resistance in men with type 2 diabetes 12周有氧抵抗运动联合训练对2型糖尿病患者化疗素、网膜和胰岛素抵抗水平的影响

Koomesh journal Pub Date : 2020-01-01 DOI: 10.29252/KOOMESH.22.1.155

M. Zarei, S. M. B. Nasr, M. Hamedınıa, H. T. Chadorneshin, Hesamedin Askari Majdabadi

{"title":"Effects of 12 weeks of combined aerobic-resistance exercise training on levels of chemerin, omentin and insulin resistance in men with type 2 diabetes","authors":"M. Zarei, S. M. B. Nasr, M. Hamedınıa, H. T. Chadorneshin, Hesamedin Askari Majdabadi","doi":"10.29252/KOOMESH.22.1.155","DOIUrl":"https://doi.org/10.29252/KOOMESH.22.1.155","url":null,"abstract":"Introduction : Regular exercise training has been shown to be a useful option to treat and prevent type 2 diabetes. Correspondingly, the purpose of this study was to investigate effects of 12 weeks of combined aerobic-resistance exercise training on levels of chemerin, omentin and insulin resistance in men with type 2 diabetes. Materials and Methods : In this clinical trial research, 20 male patients with type 2 diabetes were randomly divided into two groups combined aerobic-resistance training (n=10) and control (n=10). Subjects in training group performed 12 weeks aerobic-resistance training, 3 weekly training sessions with given intensity (aerobic: intensity of 60-70% maximum heart rate resistance: intensity 60-70% repetition maximum). Blood sampling was taken measurements of for chemerin, omentin resistance, fasting glucose and insulin resistance in the beginning and after 12 training protocol weeks were conducted. Results: After 12 weeks, in the combined training group chemerin (p=0.01), resistance insulin (p=0.01) and fasting glucose (p=0.001) decreased significantly and Vo2max increased significantly (p=0.001) than the control group. Significant change was not observed at the levels of omentin (p=0.37). Conclusion: 12 weeks of aerobic-resistance training in men with type 2 diabetes decreased the levels of chemerin, insulin resistance and also fasting glucose, but did not affect the level of omentin.","PeriodicalId":291099,"journal":{"name":"Koomesh journal","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124669790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5