Laboratornaya i klinicheskaya meditsina. Farmatsiya最新文献

{"title":"SPERM CHROMATIN DISPERSION TEST FOR EXAMINATION OF INFERTILE MALE: FROM СLINICAL TRIALS OF FIRST RUSSIAN KIT GEMSTANDART–HALOSPERM L&Q","authors":"Z. Sapozhkova, K. I. Eremin, O. Patsap","doi":"10.14489/lcmp.2022.03.pp.037-056","DOIUrl":"https://doi.org/10.14489/lcmp.2022.03.pp.037-056","url":null,"abstract":"Objectives. To evaluate the sperm chromatin dispersion in men with infertility using the first Russian kit.\u0000Methods. The study was conducted between July and August 2022. Study participants were 492 male volunteers aged 23 to 59 years with infertility which semen samples were examined by GEMSTANDART–HaloSperm L&Q kit so-called\u0000Halo test. The outcomes were as a percentage as the sperm DNA Fragmentation Index (DFI), %. The results of Halo test were evaluated according to the following threshold values: in reference range (normal): DFI  15 %, controversial result: from DFI > 15 % to < 25 %, out of reference range (pathological) DFI > 25 %. All of men (n = 492) had the results of a basic semen examination which performed a month before the current clinical trials with a various scenario of pathospermia as well as normospermia. The statistical processed a GraphPad Prism software (version 8.0.1) and Excel statistical package Microsoft® Excel® for Microsoft 365 MSO (verse 2201 16.0.14827.20158). The Pearson’s rank correlation coefficient of parametric measure and descriptive statistics have been applied.\u0000Results. Sperm DNA fragmentation (SDF) was not detected in 54.1 % (266/492 = 54.1 %) men whose average age was 36.58 years (DFI  15 %); a poor direct correlation with normal level of DFI and asthenozoospermia (r = 0.20; p < 0.0001), viscosipathy (r = 0.13; p < 0.0001) are presented; a poor inverse correlation with oligoasthenoteratozoospermia (r = – 0.12; p < 0.0001) are presented; the absence of leucospermia was noted. \u0000A controversial results for SDF presence was found in 21.3 % (105/492 = 21.3 %) men whose average age was 38.71 years (DFI range from > 15 to < 25 %); a poor direct correlation with oligoasthenoteratozoospermia (r = 0.24; p < 0.0001), oligospermia (r = 0.23; p < 0.0001) and average age of 39 years (r = 0.17; p < 0.0001) was noted; poor inverse correlation with asthenozoospermia (r = – 0.22; p < 0.0001) was presented.\u0000SDF was found in 24.6 % (121/492 = 24.6 %) men whose average age was 41.55 (DFI > 25 %); poor direct correlation with a high level of DFI and average age for infertile men in 44 years (r = 0.28; p < 0.0001) was presented. \u0000All semen samples (n = 492) presented a poor positive correlation with DFI and age (r = 0.1563; p = 0.0005); oliasthenoterato-zoospermia (r = 0.19; p = 0.0001), leucospemia (r = 0.29; p = 0.0001), and necrozoospermia (r = 0.16; p = 0.0004).\u0000Conclusion. The results of the study showed that GEMSTANDART–HaloSperm L&Q kit complements a basic semen examination providing specific information to the clinicians about quality of genetic material in semen samples.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128376330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CASE OF ANEMIA OF MIXED GENESIS WITH A DISCREPANCY IN THE RESULTS BETWEEN THE 3-DIFF HEMATOLOGICAL ANALYZER AND LIGHT MICROSCOPY","authors":"A. Shchekotova, Y. Khovaeva, L. A. Ivakina, D. Sosnin, V. Bazarnyi","doi":"10.14489/lcmp.2022.01.pp.017-032","DOIUrl":"https://doi.org/10.14489/lcmp.2022.01.pp.017-032","url":null,"abstract":"A case of severe anemia of mixed genesis is presented. The clinical picture of the disease is described, the data of laboratory investigations are given. The interest of this case for attending physicians and employees of clinical diagnostic laboratories (CDL) is due to significant discrepancies between the results of blood analysis according to automatic analysis performed using a hematology analyzer and the data of light microscopy of a stained blood smear. Various morphological changes were found in the stained blood smear, which contradicted relatively normal erythrocyte parameters (MСV, MСН, RDW) and the absence of any flags according to the hematological analyzer.\u0000The problems of using hematological analyzer data as a basis for differential diagnosis of anemia with various disorders of erythrocyte and leukocyte morphology, especially when using artificial intelligence for decision-making, are discussed.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124341661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PERSONNEL MANAGEMENT IN CONTEXT OF ANALYSING THE PHARMACISTS’ PERSONALITY TYPES","authors":"Inna Razdorskaya","doi":"10.14489/lcmp.2021.01.pp.022-028","DOIUrl":"https://doi.org/10.14489/lcmp.2021.01.pp.022-028","url":null,"abstract":"Studies have been conducted to optimize the processes of personnel management in pharmacies, to determine the relationship between the type of a pharmacist’s personality and the availability of organizational and career competencies. In order to determine the competencies necessary for the work performed, the analysis of the specifics of the pharmacists’ work (at the examples of a manager, a pharmacist with higher education, and a pharmacist with secondary professional education) has been carried out. An expert assessment of the questionnaire has been made to assess the significance of five clusters of competencies (will and leadership, interpersonal communication, organizational and strategic skills, self-positioning) in relation to the specifics of the activities at the pharmacy. The relationship between the positions of specialists and the level of development of their competencies has been determined and expressed quantitatively (from 1 to 5 points). There is a low correlation between the indicators of the competencies of the managers and pharmacists. It confirms the need for differentiation in the formation of sets of competencies for training the specialists.\u0000Based on the Lominger competencies model and the results of expert research, the ‘Personality type – position’ matrix for a pharmacy’s manager has been constructed. A personality type was determined by means of the questionnaire of Myers & Briggs test. An algorithm for determining the optimal personality type of a candidate for the position of manager has been presented. Similar calculations can be made for pharmacists with higher education and pharmacists with secondary professional education. The data obtained make it possible to use them in the determination of a working place, hiring, and rotation of specialists.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129356379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MENINGOANGIOMATOSIS AND ANGIOMATOUS MENINGIOMA: QUIZ IN DIFFERENTIAL DIAGNOSIS. A SERIES OF CASES","authors":"A. V. Nikanorova, O. Patsap, I. Grigoriev, S. A. Melchenko, I. Senko, R. A. Balgishieva, M. A. Smirnova","doi":"10.14489/lcmp.2022.02.pp.067-076","DOIUrl":"https://doi.org/10.14489/lcmp.2022.02.pp.067-076","url":null,"abstract":"Meningoangiomatosis is a rare benign lesion characterized by proliferation of blood vessels and meningothelial cells, often fusiform, which is sometimes accompanied by cortical dysplasia. Proliferation of vascular cells, pronounced cellularity and reactive gliosis may be misdiagnosed by a pathologist as invasive angiomatous meninigioma, which may lead to the postoperative radiation therapy. Immunohistochemical examination can help in the differentiation and exclusion of other diseases, such as meningioma and schwannoma. There are certain histopathological and clinical features of angiomatous meningioma, i.e. meningiomas whose vascular component exceeds 50 % of the total tumor area. Accurate differential diagnosis of meningoangiomatosis and angiomatous meningioma is important, since the correct recognition of pathology will not lead to overly aggressive postoperative treatment, as radiation therapy or chemotherapeutic treatment as in the case of meningioma.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122138200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE CONCENTRATION OF INTERLEUKIN-6 AND INTERLEUKIN-8 IN HUMAN SEMEN WITH HIGH VISCOSITY","authors":"D. Sosnin, K. R. Gal’kovich, A. Krivtsov","doi":"10.14489/lcmp.2021.01.pp.029-039","DOIUrl":"https://doi.org/10.14489/lcmp.2021.01.pp.029-039","url":null,"abstract":"Objective: to estimate the effect of ejaculate consistency on the levels of interleukin-6 and interleukin-8 in human seminal plasma.\u0000Material and methods. The concentration of interleukin-6 and interleukin-8 was determined by ELISA using the kit manufactured by «Vector-Best» (Russia). The study included 64 men: the main group (n = 30) presents patients with high semen viscosity, the comparison group (n = 34) presents men with normal semen viscosity.\u0000Results. In average, interleukin-6 level in the semen was 13.45 pg/ml, the median was 13.79 pg/ml; the data ranged from 8.24 pg/ml to 19.34 pg/ml. In average, level of interleukin-8 was 28.9 pg/ml, the median – 13.96 pg/ml; there is a large range of values from 0.202 pg/ml to 174.5 pg/ml. There are no significant differences in the values of interleukin-6 and interleukin-8 of the main group from the comparison group: for interleukin-6, U = 377.0 (p = 0.074655); for interleukin-8, U = 407.0 (p = 0.863852). The data obtained did not correlate neither between groups nor with the fertility markers of the human semen.Conclusion. Interleukin-6 and interleukin-8 levels in the human seminal plasma do not depend on semen viscosity.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122186058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GENETIC CHIMERISM IN MYELOPROLIFERATIVE NEOPLASMS: CLINICAL CASE OF COEXISTENCE OF JAK2V617F MUTATION AND BCR-ABL TRANSLOCATION","authors":"V. Bazarnyi, E. Partylova","doi":"10.14489/lcmp.2022.04.pp.033-036","DOIUrl":"https://doi.org/10.14489/lcmp.2022.04.pp.033-036","url":null,"abstract":"Essentialy, the diagnostics feature of myeloproliferative neoplasms (MPNs) is a genetic mutations. While bcr-abl translocation belongs to CML, Jak2V617F mutation is commonly associated with other MPNs. The recent decade has been clarified a data when a human blood presents bcr-abl translocation and Jak2V617F mutation both. This clinical case reports of bcr-abl translocation and Jak2V617F mutation when they were detected simultaneously by polymerase chain reaction (PCR) in a patient with myeloproliferative neoplasm, unclassifiable (MPN-U).","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123319076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BALTIC FINANCIAL AND ECONOMIC CONFERENCE 'STRATEGY OF THE DEVELOPMENT OF A MEDICAL INSTITUTION'. May 25 – 27, 2022, Svetlogorsk, RUSSIA","authors":"","doi":"10.14489/lcmp.2022.02.pp.007-010","DOIUrl":"https://doi.org/10.14489/lcmp.2022.02.pp.007-010","url":null,"abstract":"Representatives of Federal Centerof Brain Research and Neurotechnologies of FMBA of Russia took part in the Baltic Financial and Economic Conference 'Strategy for the Clinic development', which was held at the end of May 2022 in Svetlogorsk, Kaliningrad region. The event was held for health care organizers. The main changes in the current legislation regulating labor, control, supervisory and organizational issues in the field of healthcare were considered.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130863224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TERRITORIAL CLINICAL AND EPIDEMIOLOGICAL MANIFESTATIONS OF MENINGOCOCCAL INFECTION","authors":"E. V. Gorelikova","doi":"10.14489/lcmp.2022.03.pp.013-017","DOIUrl":"https://doi.org/10.14489/lcmp.2022.03.pp.013-017","url":null,"abstract":"The results of an epidemiological analysis of the incidence of meningococcal infection in the population of the Perm Region are presented. The analysis of the incidence of meningococcal infection taking into account the gender trait was carried out. The socio-age risk groups, the structure of clinical forms of meningococcal infection, serogroup characteristics of laboratory-confirmed cases, mortality from meningococcal infection were determined.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"3 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130277577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHARMACEUTICAL AND LEGAL ISSUES OF PRODUCTION AND THE USE OF INFUSIONS IN ANESTHESIOLOGY (FOR EXAMPLE, CARDIOPLEGIC SOLUTIONS)","authors":"A. I. Ovod, M. Novikova, R. Z. Simonyan","doi":"10.14489/lcmp.2022.01.pp.069-076","DOIUrl":"https://doi.org/10.14489/lcmp.2022.01.pp.069-076","url":null,"abstract":"The use of various infusion solutions in anesthesiology and intensive care involves certain risks due to the complexity of the supervised cases and the uniqueness of the interventions. Nevertheless, today reliable principles and approaches have been developed at the state level to minimize the threats associated with the quality of infusion solutions. These include the requirements for mandatory registration of all new combined solutions, including those made from already known components, as well as the rules for the production of sterile infusions according to the standard of good manufacturing practice (GMP – good manufacturing practice). Mandatory compliance with these requirements is a guarantee of the safety of infusion therapy from the standpoint of the quality of the infusion solution that is injected into the patient՚s body. \u0000On the example of cardioplegic solutions, the safety problems and contradictions in the existing system of providing high-tech field of medicine – cardiac surgery are shown.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114354632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLINICAL AND LABORATORY EXAMINATION OF SYNOVIAL FLUID IN THE JOINT DISEASES DIAGNOSTICS. PART 1","authors":"L. R. Yuldasheva, Y. A. Polevaya, Z. Sapozhkova","doi":"10.14489/lcmp.2021.02.pp.023-033","DOIUrl":"https://doi.org/10.14489/lcmp.2021.02.pp.023-033","url":null,"abstract":"Synovial fluid examination plays the important role in diagnosis and treatment of joint diseases. Nowadays it presents a substantial challenge in clinical and lab settings because of lack of manuals for the examination and processing of synovial fluid. It needed cross-disciplinary approach between orthopedic and trauma surgeons, rheumatologists, general practitioners, and clinical lab specialists to achieve the diagnostic and treatment spot-on goals in time. It is become incredibly important to develop the standard procedures for assessment of synovial fluid and its implementation into the practice for clinical diagnostic laboratories.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131834606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}