Laboratornaya i klinicheskaya meditsina. Farmatsiya最新文献

{"title":"MARKETING ANALYSIS OF MEDICINES FOR THE TREATMENT OF WOUNDS AND ULCERS","authors":"A. I. Ovod, M. D. Novikova, V. A. Solyanina","doi":"10.14489/lcmp.2022.03.pp.004-012","DOIUrl":"https://doi.org/10.14489/lcmp.2022.03.pp.004-012","url":null,"abstract":"The availability of medical care to the population at the inpatient and outpatient stages is one of the priorities of the activities of the domestic healthcare system. Treatment of patients with wounds, ulcers, wound infection requires the availability of a drug resource, including both auxiliary and specific drug therapy. A significant number of patients with skin pathologies turn to medical and pharmaceutical organizations. Their treatment is a complex and differentiated process depending on the nature and origin of the wound, as well as the individual characteristics of the victim's body. Currently, there are drugs for the treatment of this pathology on the domestic pharmaceutical market, but the most important for drug therapy for skin injuries is group D – dermatological drugs, which is represented by 21 international nonproprietary names and 283 trade names.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116764193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CASE OF PAROXYSMAL NOCTURNAL HEMOGLOBINURIA. PROBLEMS OF LABORATORY DIAGNOSTICS","authors":"D. Sosnin, A. Shchekotova, L. S. Pavlenina, P. D. Piniagina","doi":"10.14489/lcmp.2022.04.pp.037-050","DOIUrl":"https://doi.org/10.14489/lcmp.2022.04.pp.037-050","url":null,"abstract":"The presented case demonstrates the importance of a comprehensive analysis of all the examination data in a patient with paroxysmal nocturnal hemoglobinuria (PNH). A comparison was made of anamnesis data, the clinical picture of the disease, the results of biochemical blood analysis and hematological analyzer data with morphological assessment of cells in a stained blood smear. The interest of this case for hematologists and laboratory specialists is due to the rarity of this disease, difficulties in diagnostics, the presence of 2 types of hemolysis, features of morphological changes in peripheral blood erythrocytes, as well as analysis of the dynamics of manifestations, including against the background of targeted therapy of PNH.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121254716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APPLICATION OF NEURAL NETWORKS IN MEDICAL DIAGNOSTICS","authors":"V. A. Sapozhkov, O. Budadin, A. S. Churilova, B. F. Falkov, Z. Sapozhkova","doi":"10.14489/lcmp.2021.01.pp.040-051","DOIUrl":"https://doi.org/10.14489/lcmp.2021.01.pp.040-051","url":null,"abstract":"This article discusses the possibilities of application of artificial neural networks to solve problems of increasing the diagnostic outcomes in clinical laboratory examination. High diagnostic sensitivity (96 %) and diagnostic accuracy (89.5 %) of the results were shown on a large amount of cellular material digitized by artificial intelligence microscopy automation system like the Vision Cyto Pap.\u0000The high resolution and sharpness of digital slides, the mode of viewing objects (cells) in the gallery, quick access to the results of preclassification, all of these factors together allow to reduce turnearound time in more than 2.5 times reducing disadvantages of the microscopy.Application of artificial neural networks does not substitute a doctor’s skills. The role in validation of reports eligible only for cytopathologist. This concept indicates a carefully approach for staff working with a microscope, respectful attitude to them professional skills, and highlights a personalized approach to patients.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127127377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MANAGERIAL PROFESSIONALISM OF A PHARMACY’S MANAGER: THE CRITERIA OF EFFECTIVENESS","authors":"Inna Razdorskaya","doi":"10.14489/lcmp.2022.04.pp.013-019","DOIUrl":"https://doi.org/10.14489/lcmp.2022.04.pp.013-019","url":null,"abstract":"The methodological and practical foundations of forming a pharmacy’s manager managerial professionalism are presented. The relevance of research is substantiated, and the historical aspects of the origin of the institute of management in the context of managerial revolutions are analyzed. \u0000The activity of a pharmacy’s manager as a significant participant in the socially significant activity of an organization providing pharmaceutical care is analyzed. The degree of the manager’s compliance with the requirements imposed on him is different. The qualitatively determined degrees of the manager’s compliance with the requirements of professional activity are the levels of his professionalism. Two levels of professionalism are defined: the level of solving managerial tasks, which are algorithmized activities, which a manager can solve using management technologies known to him; and the level of solving managerial, non-programmed problems, and the possibility of solving them using standard technologies is difficult.\u0000The author emphasizes the need for a rational approach based on the allocation of meaningful parameters of the manager’s activity. It is proved that the competence approach is a methodological basis for designing the models of professionalism. The structure of the competence library for assessing the level of professionalism of the pharmacy’s manager is presented, including the clusters of competencies, the levels of competence development, and the indicators of behavior from ‘outstanding’ to ‘unsatisfactory’.\u0000The author proposes the criteria for the level of a manager’s effectiveness, suitable for their expression in quantitative indicators, and the tools for organizational development, that is managerial and career competencies.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123528056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VIRAL EFFECT OF SARS-COV-2 ON MALE INFERTILITY","authors":"Z. Sapozhkova","doi":"10.14489/lcmp.2021.01.pp.008-013","DOIUrl":"https://doi.org/10.14489/lcmp.2021.01.pp.008-013","url":null,"abstract":"Clinical laboratory diagnostics of male infertility is an integral part of the diagnostic process in the field of reproductive medicine. Studies conducted during the pandemia of novel coronavirus infection (COVID-19) indicate that RNA of SARS-CoV-2 can be detected in the testes, and that the possibility of disrupting spermatogenesis and the viral impact on male reproductive health has been predicted.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"192 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124282561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MULTIFACTOR ESTIMATION OF SEROPREVALENCE TO SARS-CОV-2 AMONG RESIDENTS OF THE MOSCOW REGION IN PODOLSK DISTRICT DURING THE COVID-19 EPIDEMIC PERIOD","authors":"Z. Sapozhkova, G. Milovanova, M. Smerdova, O. Patsap","doi":"10.14489/lcmp.2022.04.pp.020-032","DOIUrl":"https://doi.org/10.14489/lcmp.2022.04.pp.020-032","url":null,"abstract":"Objectives was to measure the level and to identify the structure of SARS-CоV-2 seroprevalence among residents of Podolsk district in Moscow Region during the COVID-19 epidemic period.\u0000Methods. The study was conducted between February and July 2022. Study participants were 312 volunteers from Podolsk city Moscow Region aged 6 – 80 years. A total of 312 volunteers were ranged in to five groups depending on a COVID-status (CoV-ST): group 1 – non-vaccinated-recovered, nV-Recover, 106 people; group 2 – vaccinated-non-contact, V-nC, 32 people; group 3 – recovered-then vaccinated (hybrid), R-V (hybrid), 104 people; group 4 – vaccinated-then contacted-recovered, V-R, 68 people; group 5 – non-vaccinated-non-contact, V-nC, 2 people. The amount of antibodies to SARS-CoV-2 was measured with an quantitative enzyme-linked immunosorbent assay (ELISA) kit for human blood serum tests for detecting a specific immunoglobulin G (IgG) antibodies to RBD S-protein SARS-CoV-2 (anti-IgG RBD S-protein SARS-CoV-2) with resulting a BAU “binding antibody units”. The statistical processed an GraphPad Prism software (version 8.0.1) and Excel statistical package Microsoft® Excel® for Microsoft 365 MSO (verse 2201 16.0.14827.20158). The Pearson’s rank correlation coefficient of parametric measure, descriptive statistics, Wilcoxon test for non-parametric statistics have been performed.\u0000Results. While two (2/312 = 1 %) serum samples from volunteers of group 5 were negative in anti-IgG to RBD S-protein SARS-CoV-2, the three hundred ten (310/312 = 99 %) serum samples from volunteers of group 1 – 4 were positive demonstrating the level of anti-IgG to RBD S-protein SARS-CoV-2 from 19.3 to 42.510 BAU/ml (in average 3.827 BAU/ml, median 1.990 BAU/ml, р < 0.0001). The proportion of total structure of seroprevalence which presented by 89.7 % (278/310 = 89.7 %), 65.8 % (204/310 = 65.8 %) and 55.5 % (172/310 = 55.5 %) cases respectively was introduced by ill-induced, vaccine-induced, and mixed seroprevalences from groups 1, 3 and 4; 2, 3 and 4; 3 and 4 respectively. Blood serum from 87 (87/104 = 28.1 %) seropositive volunteers of group 3 presented by level of IgG antibodies to RBD S-protein SARS-CoV-2 ranged from 500 to 42.510 BAU/ml as well as a direct-moderate correlation between COVID-19-confirmation and volunteer’s history was noted (r = 0.4, р < 0.0001).\u0000Conclusion. A high, a highest and hyperimmune response to SARS-CoV-2 which was prevailed in volunteers with CoV-ST R-V (hybrid) and was detected from 500 to > 26.000 BAU/ml reaching a maximum of 42.510 BAU/ml. These data can reflect a high level of seroconversion among a particular district of Moscow region. In groups 1 – 4 were not diagnostic significance between factors such as gender, age, severity of disease, fact and vaccination shots, vaccine manufacturing technology, time elapsed since disease and level of protective antibodies due to poorly direct or inverse correlation (r = – 0.3, p < 0.0001; r = – 0.2, p < 0.0001; r = 0.3, p","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128617987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KAZAKHSTAN INTERNATIONAL HEALTH CARE EXPO. May 18 – 20, 2022, Almaty, KAZAKHSTAN","authors":"","doi":"10.14489/lcmp.2022.02.pp.004-006","DOIUrl":"https://doi.org/10.14489/lcmp.2022.02.pp.004-006","url":null,"abstract":"In the supporting frame of the capital's business in Medicine and Pharmacy LLC Life and Quality Company/ International Cytology School&Innovatory Medical School™ (ICS&IMS™ ) which was a part of delegation of the Moscow Export Center and its collective stand MADE in MOSCOW presented innovative developments in the international arena KIHE 2022. \u0000ICS/IMS™ shared with their experience both in the field of R&D and postgraduate education of medical specialists.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123134865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCR “CHECKUP” IN MALE IN THE SEXUALLY TRANSMITTED INFECTIONS. TREND WATCHING","authors":"D. Pochernikov, Z. Sapozhkova","doi":"10.14489/lcmp.2022.03.pp.018-026","DOIUrl":"https://doi.org/10.14489/lcmp.2022.03.pp.018-026","url":null,"abstract":"Most sexually transmitted infections (STIs) do not cause significant problems in the carrier, as a result the infection is transmitted easily to a sexual partner and may remain unrecognized with serious consequences for male reproductive health. Currently, the rapidest and most accurate method of STIs diagnosis is a molecular genetic analyses (MGA) for detection of DNA pathogens. The results of MGA do not depend on the cultural properties and morphological characteristics of the microorganism. Non-cultural methods based on a multiplex polymerase chain reaction in real time (PCR-RT) are becoming as a trend in modern laboratory medicine for identifying genes associated with antibiotic resistance. \u0000his approach can reduce turnaround time (ТАТ) for the male health benefit in time.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"266 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125814056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE ESTIMATION OF NATURAL AND VACCINE-INDUCED IMMUNITY FOR PROTECTION OF NEW CORONAVIRUS INFECTION IN PERSONS WHO HAVE CONTACT WITH COVID-19. CLINICAL OBSERVATIONS","authors":"Z. Sapozhkova, G. Milovanova, O. Patsap","doi":"10.14489/lcmp.2022.01.pp.004-016","DOIUrl":"https://doi.org/10.14489/lcmp.2022.01.pp.004-016","url":null,"abstract":"Objectives. The aim of the study was to estimate the protection level by anti-IgG to RBD S-protein of the SARS-CoV-2 delivered from natural and vaccine-induced immunity in persons who have contact with COVID-19.\u0000Methods. Fourteen (14) persons presented a lab medical stuff and their relatives aged 19-72 years with several COVID-status (CoV-ST) who had contact with COVID-19 the last 10 days before blood collection was underwent to clinical trials.\u0000The CoV-ST presented by 1) non-vaccinated, recovered, nV-Recover; 2) unvaccinated, non-contact, V-nC; 3) recovered and then vaccinated (hybrid), R-V (hybrid); 4) vaccinated and then infected and recovered, V-R; 5) vaccinated, now ill, |V-Ill; 6) non-vaccinated, now ill, nV-Ill. Quantitative ELISA was conducted by anti-IgG RBD S-protein SARS-CoV-2 kit in blood serum with resulting in BAU “binding antibody units” (First WHO International Standart for anti-SARS-CoV immunoglobulin (human), NIBSC code: 20/136). The estimation was performed according manual for kit and immunoassay analyser in optical density of solutions at one main wavelength of 405 nm. The Spearman’s rank correlation coefficient of nonparametric measure was used due to small numerous of cohort. Statistical analyses was calculated with GraphPad Prism 8.0.1 and Microsoft® Excel® for Microsoft 365 MSO (verse 2201 16.0.14827.20158) programs.\u0000Results. All fourteen (14) persons who had contact with COVID-19 was immunized. Three (3) female persons aged less than 30 years had anti-IgG RBD S-protein SARS-CoV-2 in diapason from 19.2 till 230.1 BAU/mL which was infected and got ill with might-to-moderate symptoms of COVID-19 (f,34 nV-Recover; f,33 nV-Ill; f,22 V-Ill). Eleven (11) persons which was not infected and got ill 1) six (6) with R-V (hybrid) and vector technology vaccine had anti-IgG < 500 BAU/ml; 2) eight (8) persons, seven (7) of them with R-V (hybrid), vector and mRNA-technology vaccines had anti-IgG >500 BAU/ml; 3) four (4) persons with R-V (hybrid), one (1) of them had a vector technology vaccine, three (3) of them had an mRNA technology vaccine had anti-IgG > 5000 BAU/ml; 4) the highest concentration of specific anti-IgG 50,000 BAU/ml was detected in 19-year-old men with R-V (hybrid) and mRNA technology vaccine.\u0000Conclusion. Direct moderate and moderate inverse correlation was noted between many factors which can determine an adaptive human immunity. The observe study showed a rapid immune response to neutralization of viruses in medical stuff and their relatives who had closest contact with COVID-19, aged 19 to 50 years and who previously was ill and subsequently was vaccinated triply by vector vaccine or previously was ill and then was vaccinated twice by mRNA vaccine.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"156 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122611690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE STATUS OF HPV TESTING IN THE DIAGNOSIS OF CERVICAL CANCER AND HEAD AND NECK TUMORS: EXCHANGE OF FOREIGN EXPERIENCE","authors":"G. Milovanova, K. Kasoyan, O. Patsap","doi":"10.14489/lcmp.2022.01.pp.040-055","DOIUrl":"https://doi.org/10.14489/lcmp.2022.01.pp.040-055","url":null,"abstract":"Eastern Europe and Russia belong to regions with a high level of HPV infection – about 22 % of the population are infected, while there is an uneven distribution of virus species by region and social groups. The most common oncological diseases diagnosed together with HPV are anal cancer – 80 %, vaginal cancer – 70 %, oral and pharyngeal cancer – 30 %, penile cancer – 50 %. Using the example of foreign experience in the diagnosis of cervical cancer and squamous cell carcinoma of the head and neck, the role of various laboratory methods and their combinations in the diagnostic search for human papillomavirus of high carcinogenic risk is shown. The review presents diagnostic capabilities of genotyping, immunocytochemical staining of p16/Ki-67 markers, DNA methylation methods, in situ hybridization on cell blocks, molecular methods of nucleic acid amplification, as well as an express method for determining oncoprotein E6.","PeriodicalId":287737,"journal":{"name":"Laboratornaya i klinicheskaya meditsina. Farmatsiya","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127919063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}